• The Plant Pathology Journal
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• v.22 no.1
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• pp.21-27
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• 2006

We immunized BALB/c mice with purified Cucumber green mottle mosaic virus isolate HY1 (CGMMV-HY1). Through the selection of positive clones that were grown on the HAT medium, four sensitive monoclonal clones (CG99-01, CG99-02, CG99-03, and CG99-04) were selected from 500 Hypoxanthine-guanine phosphoribosyltransferase positive hybridoma cells. Four sensitive clones of CGMMV-HYI were determined as IgM type of the subclass of mouse immunoglobulins Ig group. The titer of monoclonal antiserum against CGMMVHY1 was estimated 1:12,800 by the indirect ELISA. Although monoclonal antibodies (MAbs) from CG99-01 and from CG99-04 cross-reacted with Zucchini green mottle mosaic virus and Kyuri green mottle mosaic virus, MAb from the cell line CG99-03 was highly specific to CGMMV. No MAbs cross-reacted with Cucumber mosaic virus-Fny. Only CG99-04 reacted with Pepper mild mottle virus weakly and CG99-02 reacted with both CGMMV and KGMMV. CGMMV was detected from the rind of watermelon fruit by DAS-ELISA of CGMMV-HY1, but not from the flesh of watermelon. Average seed transmission rate of CGMMV in watermelon was $24\%$ from symptomatic watermelon collected from 5 regions of Gyeongnam province. CGMMV was detected by DAS-ELISA with specific MAb of CGMMVHY1 periodically from root stock, during the sequential process for nursery seedling in Haman. Necrotic spots on cotyledons of root stock seedling progressed to reveal the typical symptomatology on the primary leaves of scion upon grafting. Here, we have established MAb based ELISA system, which could accurately detect CGMMV from watermelon seeds, nursery seedlings, transplants and field samples from greenhouse or open out door field as well.

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1644-1649
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• 2009

This study investigates the buffering effects of calcium salts in kimchi on the total acidity, microbial population, and dextransucrase activity. Calcium chloride or calcium carbonate was added to dongchimi-kimchi, a watery radish kimchi, and the effects on various biochemical attributes were analyzed. The addition of 0.1% calcium chloride produced a milder decrease in the pH after 24 days of incubation, which allowed the lactic acid bacteria to survive longer than in the control. In particular, the heterofermentative Leuconostoc genus population was 10-fold higher than that in the control. When sucrose and maltose were also added along with the calcium salts, the dextransucrase activity in the kimchi was elevated and a higher concentration of isomaltooligosaccharides was synthesized when compared with the control. Calcium chloride was determined as a better activator compound of dextransucrase than calcium carbonate, probably because of its higher solubility. Therefore, the results of this study confirm the ability of the proposed approach to modulate the kimchi fermentation process and possibly enhance the quality of kimchi based on the addition of dietary calcium salts.

• The korean journal of orthodontics
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• v.23 no.1 s.40
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• pp.17-35
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• 1993

We tried to evaluate frequencies and severities of malocclusion of various socioeconomic areas according to growth and development. To obtain objective validity, we used Handicapping Labiolingual Deviation (HLD) suggested by Draker, Treatment Priority Index (TPI) by Grainger, Handicapped Malocclusion Assessing Record (HMAR) by American Dental Association and American Association of Orthodontist. Seoul and Kwangju were selected as an urban group, Ahnyang as an middle socioeconomic group and Hwasun near the Kwangju area as an rural group. 140 (male 63, female 73) of 5th grade in 'E' elementary school students in Seoul, 202 (male 101, female 101) of 'S' elementary school students in Kwangju, 207(male 105, female 102) of 'H' elementary school students in Hwasun, and 100(male 49, female 51) of 'M' elementary school students of in Ahnyang of all the same grade were analyzed and we obtained the results as follows; 1. TPI, HLD, and HMAR showed high coincidence and reproducibility between different observer. (p<0.01) 2. In comparison of TPI, HLD, and HMAR according to clinical decision, there was statistically difference between each other. (p<0.01) 3. As the severity of malocclusion increases there was high correlation between TPI and HLD, and between HLD and HMAR (p<0.05) 4. With respect to differences between areas in TPI and HLD, there was statistically difference between Ahnyang and other areas. 5. In Seoul as an urban group there was a high incidence of periodic checkup and history of orthodontic treatment than other area. 6. Mode of feeding and posture during sleeping did not affect the severity of malocclusion. As a conclusion, TPI, HLD and HMAR are so reproducible and coincident that they can be used as a guide on evaluating the frequency and severity of malocclusion and determining the priority of orthodontic treatment to determine the need and supply of orthodontic treatment. Furthermore these indices can provide objectively valid data for establishing public health problem solution.

• Journal of The Korean Association of Information Education
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• v.12 no.2
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• pp.163-171
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• 2008

To cope actively in the knowledge based society, we deepen a specialty territory and magnify this by applying Information Communications Technology(ICT) effectively. It needs the ability that it will be able to solve the problem from daily life effectively. Accordingly Ministry of Education & Resource Development of Korea make an effort for ICT application education. In spite of this effort, digital divide problem is serious socially and the computer subject is not adopted with formal subject from school. In this study, we analyzed the computer application ability gap and recognized the importance of computer education by analyzing the difference in computer application ability between the students who take a computer class and who do not take a class. Also by comparing the difference of computer application ability between the city and rural student, we tried to find a solution to reduce the regional disparity.

• Journal of the Korean Geographical Society
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• v.36 no.4
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• pp.444-457
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• 2001

Present work aims to analyse the spatio-temporal distribution of Korean bamboos. The northern limit of Korean bamboo species occurs at Baekryung Island(124°10'E, 37°55'N), Hoochang(41°22'N) and Myungchon(129°41\`E, 41°10'N). The presence of bamboo fossils in Korea dates back from Tertiary, but widespread use of bamboos can be noticed since 1454. Reconstructed ranges and temporal changes of bamboo, based upon the eight historical records from 15th to 20th centuries, indicates that both climatic and anthropogenic factors were responsible for the changes of distributional range. The spread of bamboos was balanced by its disappearance due to regional development, and the total bamboo area showing little change. Due to the tendency to spread extensively by means of underground stems, bamboos often invade adjacent forests and disturb natural vegetation. Proper management and conservation strategies, therefore, are required for the maintenance of rural landscape.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.12 no.6
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• pp.123-138
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• 2017

This study aims to identify the characteristic variables of businesses that would impact the choice of their type in the 6th industry and analyze how they work. To this end, this study analyzed data of 752 businesses certified as belonging to the 6th industry in 2015 through the classification and regression tree (CART) algorithm in decision tree analysis. The results of analysis showed that the type of agricultural product processing affected shaping the type of the 6th industry at the early stage of growth while the type of agricultural product processing, the type of service, region and sales volumes at the stage of growth and service strategy and the type of agricultural product processing at the stage of maturity. These findings empirically identified key business factors that could support businesses in the 6th industry at each stage of growth and presented a direction forward for support of the 6th industry.

• Journal of Korean Society for Geospatial Information Science
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• v.21 no.1
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• pp.3-9
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• 2013

Development of housing, industrial and public lands has been a cause to increase a temperature of a city higher than that of a rural area. Knowing that, the research analyzed temperature changes in a riverside district and a coastal area depending on the land usage and climate change in the areas. Add to this, the study examined a correlation between topographic characteristics and temperature changes. According to the results, the temperature was increased by $1.80^{\circ}C$ in 2007 comparing to 2003 while the wind velocity was reduced by 0.24m/s. In addition, a more number of areas were designated as build-up areas which, in return, decreased the forested land. The analysis on the correlation reported that the riverside district has more of grassland and bare land while the coastal area has more of wooded land and grassland. The study is expected to be used as fundamental data for research on heat island effect which would be different by each regional characteristic as it proposes measures to solve the heat island effect.

• Journal of the Korean Institute of Landscape Architecture
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• v.35 no.4
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• pp.81-89
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• 2007

Urban landscape represents a city's internal identity as well as external form. Therefore, urban landscape and image planning has to involve marketing strategies to deliver the economic growth and regional revitalization through recreating the environmental and cultural value of the city. This study intends to develop practical city image management programs as city marketing tools which consider opinions of local authorities, related experts, and residents. To achieve this purpose conjoint analysis was applied on three components which symbolically reflects features of assets and resources in Anseong. This study can be summarized as follows: 1. The first consideration for Lake Geumgwang is to develop farm experience Programs on waterside fields. 2. The rot consideration for Bauduki Festival is to make efforts for public relations(PR) to be able to merchandise festival-related goods and develop unique products. 3. The first consideration for Anseong Grapes is to build leisure-oriented theme parks for local farm products. This study is meaningful to develop the efficient and quantitative management method of city image for marketing. The integrated approach to the management programs to reflect opinions of local policies, experts and citizens is expected to become a useful foundation in studying city images and marketing.

• The Plant Pathology Journal
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• v.29 no.3
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• pp.234-241
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• 2013

Polygalacturonase (PG) gene is a typical gene family present in eukaryotes. Forty-nine PGs were mined from the genomes of Neurospora crassa and five Aspergillus species. The PGs were classified into 3 clades such as clade 1 for rhamno-PGs, clade 2 for exo-PGs and clade 3 for exo- and endo-PGs, which were further grouped into 13 sub-clades based on the polypeptide sequence similarity. In gene structure analysis, a total of 124 introns were present in 44 genes and five genes lacked introns to give an average of 2.5 introns per gene. Intron phase distribution was 64.5% for phase 0, 21.8% for phase 1, and 13.7% for phase 2, respectively. The introns varied in their sequences and their lengths ranged from 20 bp to 424 bp with an average of 65.9 bp, which is approximately half the size of introns in other fungal genes. There were 29 homologous intron blocks and 26 of those were sub-clade specific. Intron losses were counted in 18 introns in which no obvious phase preference for intron loss was observed. Eighteen introns were placed at novel positions, which is considerably higher than those of plant PGs. In an evolutionary sense both intron loss and gain must have taken place for shaping the current PGs in these fungi. Together with the small intron size, low conservation of homologous intron blocks and higher number of novel introns, PGs of fungal species seem to have recently undergone highly dynamic evolution.

• Korean Journal of Agricultural and Forest Meteorology
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• v.22 no.3
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• pp.128-134
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• 2020

The daily gridded meteorological information and climatology with high resolution (30m and 270m) was produced from 94 Automated Surface Observing System (ASOS) of Korea Meteorological Administration (KMA) for the past 50 years (1971-current) by different downscaling methods. In addition, the difference between daily meteorological data and the mean state of past 30 years (1981-2010) was calculated for the analysis of climate change. These datasets with GeoTiff format are available from the web interface (https://agecoclim. agmet.kr). The performance of the data is evaluated using 172 Automatic Weather S tation (AWS ) of Rural Development of Administration (RDA). The data have biases lower than 2.0, and root mean square errors (RMSE) lower than 3.8. This data may help to better understand the regional climatic change and its impact on agroecosystem in S outh Korea.