• 동의생리병리학회지
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• 제17권4호
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• pp.962-968
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• 2003

The purpose of this research was to investigate inhibitory activity of Sasang medicines on diabetes. Reduce 36 kind of medicines to powder, abstract add 100 times methanol. Examine inhibitory activity on α-amylase and α-glucosidase in vitro. Thirty six oriental herbs were screened for inhibitory activities against carbohydrate digesting enzymes in vitro. Methanol extract of Pleuropterus multiflorus Turez, Ephedra sinica, Moutan cortex Radicis, Magnolia obovata, Alpinia officinarum, Amomi Semen, Rubus coreanus, and Cinnamomi cortex inhibited yeast α-glucosidase activity and extract of Moutan cortex Radicis and Rubus coreanus inhibited porcine α-amylase activity by more 50%. Rubus coreanus showed the strongest inhibition against both α-glucosidase and α-amylase. Therefore, Rubus coreanus was the most effective medicice of 36 kind of medicines.

• Food Science and Biotechnology
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• 제17권2호
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• pp.251-256
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• 2008

Changes in antioxidant activity of Rubus coreanus fruit of 3 clones (S13, S114, and S16), which were selected from different sites, were studied at different ripening stages. Antioxidant activities (tree radical scavenging activity and reducing power) were determined and their relationships to total phenolic contents and ascorbic acid were analyzed. The highest tree radical scavenging activities of 3 clones (S13, S14, and S16) were 79.39, 75.80, and 81.16% at $125\;{\mu}g/mL$, respectively. In general, the antioxidant activity and the related parameters, including total phenolic content and vitamin C content decreased during fruit ripening. Total phenolic contents of the R. coreanus fruits (S13, S14, and S16) were correlated with tree radical scavenging activity ($R^2=0.8114$, 0.9186, and 0.9714). These results improve knowledge of the effect of ripening on the antioxidant activity and related compounds contents that could help to establish the optimum R. coreanus fruit harvest data for various usages.

• 한국자원식물학회지
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• 제27권6호
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• pp.593-600
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• 2014

Osteoporosis is a progressive bone disease characterized by low bone mass which is caused by disturbance in the balance between the activities of osteoblasts and osteoclasts. Postmenopausal osteoporosis is one of the most common disorders in women after menopause, which is linked to an estrogen deficiency and characterized by an excessive loss of trabecular bone. Rubus coreanus has been used for their various pharmacological properties in Asia as a traditional medicine. To investigate the effect of unripe fruits of R. coreanus 30% ethanol extract (RCE) on osteoblast-like cells (MG63) differentiation, we examined the effects of RCE on in vitro osteoblastic differentiation markers, alkaline phosphatase (ALP) activity and receptor activator of nuclear factor ${\kappa}$-B ligand (RANKL) and osteoprotegerin (OPG) expression. The high concentration (50 and $100{\mu}g/mL$) of RCE markedly increased ALP activity, whereas decreased the RANKL/OPG. We also investigated the effect of RCE on M-CSF plus RANKL-induced differentiation of pre-osteoclast cells (RAW 264.7). RCE treatment remarkably inhibited M-CSF/RANKL-induced formation of osteoclast-like multinuclear cells from RAW 264.7 cells. Moreover, the inhibitory effect of RCE was reduced by selective estrogen receptor-${\alpha}$ antagonist. Our research suggests that suggested that unripe fruits of R. coreanus may act beneficial effects on bone mass by regulating both osteoblast and osteoclast.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.39-44
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• 2008

Korean cultivated bramble, which is known as Bokbunja-ddal-gi is regarded to be originated from Korea native Rubus coreanus. However, little scientific evidence and significant morphological differences between Korean cultivated bramble(KCB) and R. coreanus throw doubt on the ancestry of KCB. This study was carried out to obtain phylogenetic information on KCB by comparing its nuclear genomic background with those of R. coreanus, black(R. occidentalis) and red(R. idaeus) raspberry, blackberry(R. lanciniatus) and R. crataegifolius. A total of 99 random amplified polymorphic DNA(RAPD) markers were generated and used for phylogenetic analysis of 76 Rubus accessions. Accessions of each species were grouped into each distinct subclade by the RAPD markers at a similarity coefficient of about 0.59. The KCB subclade formed a clade with R. occidentalis and R. crataegifolius subclades at a similarity coefficient of 0.47. The R. coreanus subclade formed a clade with R. idaeus, R. lanciniatus and R. crataegifolius subclades at a similar similarity coefficient. Only one KCB accession from Hoengsung was included in R. coreanus subclade. The accession shows leaf and flower characteristics different from the rest of the KCB accessions. The phylogenetic relationship inferred from the RAPD markers suggests that the nuclear genomic background of KCB accessions which show morphological similarity to black raspberry is more closely related to black raspberry than to R. coreanus. This brings about the need for close scientific evaluations on the ancestry of KCB at both morphological and molecular levels.

• 생약학회지
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• 제18권3호
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• pp.188-190
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• 1987

A triterpene glycoside was first isolated from berries of Rubus coreanus Miquel in the family of Rosaceae, which have been used as a tonic in chinese drug. The compound was identified as $28-{\beta}-D-glycopyranosyl$ ester of $2{\alpha},3{\beta},19{\alpha},23-tetrahydroxyurs-12-en-28-oic$ acid by IR, MS, $^{1}H-NMR$, $^{13}C-NMR$ spectra.

• Natural Product Sciences
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• 제15권3호
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• pp.139-143
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• 2009

The anaphylactic allergic reaction is involved in many allergic diseases such as asthma and allergic rhinitis. In this report, I investigated the effect of ripe fruits of Rubus coreanus Miq. (Rosaceae) (RFRC) on the anaphylactic allergic reaction and studied its possible mechanisms of action. RFRC inhibited compound 48/80-induced systemic anaphylactic reaction in mice. RFRC dose-dependently decreased the IgE-mediated passive cutaneous anaphylaxis. In addition, RFRC decreased the gene expression and production of tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and interleukin (IL)-6 in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187 (A23187)-stimulated human mast cells. These findings provide evidence that RFRC could be a candidate as an anti-allergic agent.

• 한국자원식물학회지
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• 제19권3호
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• pp.381-384
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• 2006

This study was conducted to selected Korean black raspberry (Rubus coreanus Miq.) for high productivity. The eight major agronomic traits were investigated from 198 clones of the clone bank established in Korea Forest Research Institute, Suwon, Korea. The selection levels based on number of fruit per fructify lateral (NFFL) over 20, and fruit weight (FW) over 1.3g, and yield of individual per fructify lateral (YIFL) over 25g, were applied on 198 clones, resulted in 17 clones selected. The selected superior trees, 17 clones, appeared regional differences for amount of fruiting among 4 different test sites. When number of fruit per fruit petiole (NRFP), fruit weight (FW), yield of individual (YI) and sugar content were satisfied over 20, 1.4g, 6kg and 9.5 brix, respectively, as a select condition, 5 clones were reselected as the superior trees among 17 clones. for 3 years.

• 약학회지
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• 제43권4호
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• pp.458-463
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• 1999

This study was undertaken to test for antiviral activity of the aqueous extracts prepared from 4 medicinal plants of Korea (Terminalia chebula, Sanguisorba officinalia, Rubus coreanus, Rheum palmatum). Aqueous extracts were assayed for the inhibition of hepatitis B virus (HBV) replication by measurement of HBV DNA and surface antigen (HBsAg) levels in the extracellular medium of HepG2 2.2.15 cells. All extracts decreased the levels of extracellular HBV virion DNA at concentrations ranging from 64 to $128{\;}\mu\textrm{g}/ml$ and inhibited the production of HBsAg dose-dependently. Among the 4 tested plants, Terminalia chebula exhibits the most prominent anti-HBV activities. Our findings suggest that these 4 medicinal plants may have potential to develop as specific anti-HBV drugs in the future.

• 한국식품영양과학회지
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• 제43권9호
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• pp.1357-1362
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• 2014

본 연구에서는 토종 복분자와 외래종 복분자의 항산화 활성과 장세포 산화스트레스 보호의 차이를 알아보기 위해 총 폴리페놀 및 플라보노이드 함량, DPPH 및 ABTS 라디칼소거능, FRAP assay를 이용한 환원력을 비교 조사하였고, 사람의 장내 세포인 Caco-2 cell에 과산화수소를 이용하여 자극시켜 장세포 산화스트레스 보호를 확인하였다. 총 폴리페놀 및 플라보노이드 함량은 외래종 복분자가 유의적으로 높은 것으로 나타났다. ABTS 라디칼 소거능과 FRAP assay을 이용한 환원력에서도 외래종 복분자가 유의적으로 높은 것으로 나타났으나 DPPH 라디칼 소거능은 토종과 외래종 복분자 모두 비슷하게 나타났다. 장세포 산화스트레스 보호 역시 외래종 복분자가 유의적으로 높게 나타났다. 항산화 성분과 항산화 활성의 상관관계는 모두 유의하게 양의 상관관계를 보이는 것으로 나타났으며, FRAP value가 가장 높은 상관관계를 보이는 것으로 확인되었다. 따라서 FRAP value가 높았던 외래종 복분자 100% EtOH 추출물이 폴리페놀 함량, 플라보노이드 함량, ABTS 라디칼 소거능, DPPH 라디칼 소거능, 장세포 산화스트레스 보호능 모두 높은 추출물로 확인되었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 춘계학술발표회
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• pp.95-95
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• 2018

Uric acid is the end product of purine metabolism in human body, originating from hypoxanthine after enzyme catalysis by Xanthine oxidase (XOD). Hyperuricemia results as a result of either over-generation of uric acid or a reduction in its excretion. In silico modelling methods such as Absorption, Distribution, Metabolism, Excretion and Toxicity (ADMET) prediction, Autodock 4.2.6 program were used to study the potential inhibitory compounds of XOD. Also we investigated the inhibition of XOD activity by using the extracts of Dendropanax morbifera and Rubus coreanus Miq spectrophotometrically. According to ADMET data, several compounds from D. morbifera and R. coreanus plants, were found to be more potent in inhibiting the XOD activity than allopurinol. XOD inhibitory activity is evaluated by quantifying the formation of uric acid by measuring the absorbance at 290 m ($A_{290}$).D. morbifera extract inhibited XOD activity at $250{\mu}g/ml$, however the extracts from R. coreanus has inhibited XOD activity at $25{\mu}g/ml$. The major compound of R. coreanus, ellagic acid significantly increased the inhibition rate from $9{\mu}g/ml$ and showed a 71% suppression rate at $15{\mu}g/ml$. Finally, these results suggested a potential inhibitory activities of the extracts from D. morbifera and R. coreanus Miq, but further research is needed to validate to ensure their safe usage as drug.