YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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Inhibitory Effects of Terminalia chebula, Sanguisorba officinalis, Rubus coreanus and Rheum palmatum on Hepatitis B Virus Replication in HepG2 2.2.15 Cells

HepG2 2.2.15 세포주를 이용한 가자, 지유, 복분자, 대황의 B형 간염바이러스 증식 억제 효과

  • Published : 1999.08.01
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Abstract

This study was undertaken to test for antiviral activity of the aqueous extracts prepared from 4 medicinal plants of Korea (Terminalia chebula, Sanguisorba officinalia, Rubus coreanus, Rheum palmatum). Aqueous extracts were assayed for the inhibition of hepatitis B virus (HBV) replication by measurement of HBV DNA and surface antigen (HBsAg) levels in the extracellular medium of HepG2 2.2.15 cells. All extracts decreased the levels of extracellular HBV virion DNA at concentrations ranging from 64 to $128{\;}\mu\textrm{g}/ml$ and inhibited the production of HBsAg dose-dependently. Among the 4 tested plants, Terminalia chebula exhibits the most prominent anti-HBV activities. Our findings suggest that these 4 medicinal plants may have potential to develop as specific anti-HBV drugs in the future.

Keywords

References

  1. Microbiol. Immunol v.40 no.2 Colorimetric assay system for screening antiviral compounds against hepatitis B virus Sudo, K.;Konno, K.;Shigeta, S.;Yokota, T.
  2. Nature v.317 The hepatitis B virus Tiollas, P.;Pourcel, C.;Dejean, A.
  3. Lancet v.342 Clinical aspects of hepatitis B virus infection Wright, T. L.;Lau, J. Y. N.
  4. Prog. Med. Virol v.40 Progress and per-spectives in human heptitis B cirus research Yoffe, B.;Noonan, C. A.
  5. Annu. Rev. Med v.45 Molecular events in the patho-genesis of hepanavirus-associated hepatocellular carcinoma Robinson, W. S.
  6. Hepatology v.14 Antiviral agents for hepatitis B Gerin,J. L.
  7. Proc. Natl. Acad. Sic. USA v.84 Hepato-carcinogenicity of the woodchuck hepatitis virus Popper, H.;Roth, L.;Purcell, R. H.;Tennant, B. C.;Cerin, J. L.
  8. Antiviral Res v.15 A cell culture assay for compounds which inhibit hepatitis B virus replication Korba, B. E.;Milman, G.
  9. Antiviral Res v.19 Use of a standardized cell culture assay to assess activity of nucleoside analogs against hepatitis B virus replication Korba, B. E.;Gerin, J. L.
  10. Antimicrob. Agents Chemother v.37 High-capacity in vitro assessment of anti-hepatitis B virus compound selectivity by a virion-specific polymerase chain reaction assay Jansen, R. W.;Johoson, L. C.;Averett, D. R.
  11. J. of Hepatology v.22 Antiviral agents in hepatitis B cirus transfected cell lines: inhibitory and cytotoxic effect related to time of treatment Kruining, J.;Heijtink, R. A.;Schalm, S. W.
  12. 본초약재도감 전통의학연구소편
  13. 도해향약대사전(식물편) 정보섭;신민교
  14. Phytotherapy Res v.9 Investigation of Korean plant extracts for potential phyotherapeutic agents against B-virus hepatitis Chung, T. H.;Kim, J. C.;Kim, M. K.;Choi, S. C.;Kim, S. L.;Chung, J. M.;Lee, I, S.;Kim, S. H.;Hahn, K. S.;Lee, I. P.
  15. Phytotherapy Res v.11 Pothential antiviral effects of Terminalis chebula, Sanguisorba officinalis, Rubus coreanus and Reum Palmatum agains duck hepatitis B virus (DHBV) Chung, T. H.;Kim, J. C.;Lee, C. Y.;Moon, M. K.;Choi, S. C.;Lee, I. P.
  16. Kor. Biochem.J v.17 Cloning and expression of hepatitis B virus surface antigen gene Kim, Y.;Kang, H. S.
  17. The Molecular Medicine of Viral Hepatitis Harrison, T. J.;Zuckerman, A. J.
  18. Antiviral Res v.27 Effecacy of traditional herbal medicines in combination with acycovir agaist herpes simplex virus type 1 infection in vitro and in vivo Kurokawa, M.;Nagasaka, K.;Hirbayashi, T.;Uyama, S.;Sato, H.;Kageyama, T.;Kadota, S.;Ohyama, H.;Hozumi, T.;Namaba, T.
  19. Antiviral Res v.32 Prophylactic treatment of cytomegalocirus infection with traditional herbs Yukawa, T. A.;Kurokawa, M.;Sato, H.;Yoshida, Y.;Kageyama, S.;Hasegawa, T.;Namba, T.;Imakita, M.;Hozumi, T.;Shirake, K.
  20. Chem. Pharm. Bull. (Tokyo) v.43 Inhibitory effects of Egyptian folk medicines on human immunodficiency virus (HIV) reverse transcriptase el-Mekkaway, S.;Meselhy, M. R.;Kusumoto, I. T.;Kadota, S.;Hattori, M.;Namba, T.