• Molecules and Cells
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• 제24권2호
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• pp.194-199
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• 2007

GCN4 is a typical eukaryotic transcriptional activator that is implicated in the expression of many genes involved in amino acids and purine biosyntheses under stress conditions. It is degraded by 26S proteasomes following ubiquitination. However, the immediate receptor for ubiquitinated Gcn4p has not yet been identified. We investigated whether ubiquitinated Gcn4p binds directly to Rpn10p as the ubiquitinated substrate receptor of the 26S proteasome. We found that the level of Gcn4p increased in cells deleted for Rpn10p but not in cells deleted for RAD23 and DSK2, the other ubiquitinated substrate receptors and, unlike Rpn10p, neither of these proteins recognized ubiquitinated Gcn4p. These results suggest that Rpn10p is the receptor that binds the polyubiquitin chain during ubiquitin-dependent proteolysis of Gcn4p.

• 대한영양사협회학술지
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• 제21권1호
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• pp.25-36
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• 2015

In this study, we attempted to improve hospital food delivery service quality and customer satisfaction by using FMEA (Failure Mode and Effect Analysis), which is applied to the quality control of products in manufacturing plants. Subjective food delivery service quality improvement was judged based on a 5-point likert scale. Traditional FMEA uses an RPN (Risk priority number) to evaluate the risk level of a component or process. The RPN index was determined by calculating the product of severity, occurrence, and detection indexes. In our results, total RPN value (P<0.01) significantly decreased after FMEA introduction, whereas customer satisfaction (P<0.001) and food delivery service quality (P<0.001) significantly increased. Specifically, foodservice errors (P<0.01) and loss cost (P<0.01) were significantly improved by FMEA introduction. Taken together, we suggest that FMEA reduces critical activities and errors in foodservice delivery caused by simple priority selection.

• 대한방사선기술학회지:방사선기술과학
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• 제47권4호
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• pp.287-294
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• 2024

Radiation oncology departments are at high risk for potential radiation safety incidents. This study aimed to identify risk factors for these incidents using the P-mSHEL (Patient, Management, Software, Hardware, Environment, and Liveware) model and to evaluate potential accident types through Failure Mode and Effects Analysis (FMEA). FMEA identified seven accident types with high Risk Priority Number (RPN). A total of 56 detailed risk factors were classified using the P-mSHEL model, and measures to prevent radiation safety incidents were implemented. The effect of these preventive measures on workers' safety perception was confirmed through two indicators (FMEA and safety perception). After implementing the preventive measures, the FMEA analysis showed that the highest reduction in RPN was for A-6 (radiation exposure while other patients/guardians are present) with a reduction rate of 33.3%, followed by B-3 (radiation exposure while staff are present) with a reduction rate of 33.3%. Overall safety perception significantly improved after the preventive measures (4.17±0.35) compared to before (2.76±0.33) (p<0.05), with notable increases in both employee safety culture (3.93±0.51) and patient safety culture (3.73±0.62) (p<0.05). This study identified risk factors in radiation oncology departments. Continuous management, maintenance, and fostering a strong safety culture are crucial for preventing incidents. Regular problem identification and collaboration with relevant departments are essential for maintaining safety standards.

• KSBB Journal
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• 제8권3호
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• pp.217-223
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• 1993

호알카리성 Alcaligenes속의 한 균종응 이용하여 peptide계 생분해성 고분자인 ${\gamma}$-PGA( p이yglutarnic a acid}의 회분 맺 연속배양어l 의한 대량 생산성을 조사하였다. 화분배양시 ${\gamma}$-PGA의 생생량 벚 생산생은 포도당 농도 50g/P 까지 포도당의 농도증가에 따라 증가하였으나 최대 생산수율은 10gjP 의 포도당 농도 에서 얻어졌으며, 이때의 수율은 63% 이였다. 또, 비 증식속도는 포도당 농도 50g/l 에셔 약 $0.25hr^{-1}$로 최대값을 나타냈으나, 이 이상의 농도에서는 고농도 기섣에 의한 균체생육의 저해륜 나타내였다. 온도, pH 및 교반속도(rpm)를 독랩변수로 한 반응표면 분 석에 의한 ${\gamma}$-PGA 생산의 최적 희분배양 조건은 배 양온도 $31^{\circ}C$, pH 10 및 회전속도 87rpm의 정상점에 서 얻어졌으며, 이 조건에서 ${\gamma}$-PGA의 생산량은 약 11g/l 로 추정되었다. 연속배양의 경우 wash out은 희석률 0.5hr-1에서 일어났으며, 희석률 $0.40hr^{-1}$일 때 최대 생산성을 나타내였고, 최대 생산생은 2.80g/l/h로 회분배양보다 약 9배 높았다.

• 한국건축시공학회지
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• 제16권6호
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• pp.519-527
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• 2016

최근 건설 기업들이 질적 성장을 필요로 하면서 리스크 분석 요구가 증가하고 있다. 하지만 건설 현장의 정량적인 데이터를 활용하여 손해 범위로 리스크 분류를 진행하는 연구는 미비한 실정이다. 따라서 본 연구에서는 건설 현장의 사고원인에 따른 내 외부 리스크 핵심 요인을 분석하는 것을 목표로 한다. 이를 위해 본 연구에서는 A보험사에서 실제 12년간 발생된 사고 데이터를 329개 수집하였다. 우선 수집한 데이터를 토대로 내 외부 발생 위험도에 따른 사고 원인별 중요도 차이를 분석하기 위해 RPN 기법을 수행하였다. 또한, 이러한 차이가 유의미한지를 분석하기 위해 T-test분석을 활용하여 내 외부 발생 위험도 간의 피해율 차이를 검증하였다. 그 결과, 내 외부 발생 위험도 간의 차이는 유의미한 것으로 나타났으며, 특히 480억 미만의 건설 현장의 사고에서는 시공결함과 진동 소음 먼지가, 480억 이상의 대규모 건설 프로젝트의 현장에서는 호우, 태풍이 주요사고 원인으로 이에 대한 대처 방안이 필요한 것으로 나타났다. 본 연구의 결과는 향후 건설 공사 현장에서 리스크 관리를 수행할 때, 내 외부에 따른 체계적인 리스크 식별 데이터로 활용이 가능할 것으로 기대된다.

• 대한안전경영과학회지
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• 제25권3호
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• pp.73-81
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• 2023

This study uses FMECA (Failure Modes, Effects, and Criticality Analysis) and HAZOP (Hazard and Operability), which are widely applied in industrial areas, among risk assessment methods, and applies them to the same system. While FMECA evaluates system failure conditions and analyzes risks, HAZOP evaluates the system comprehensively by evaluating operational risks that may occur based on system parameters. According to data released by the Ministry of Land, Infrastructure and Transport, as of December 2021, the length of roads in Korea is 113,405 km, and the repair of guardrails that have expired must be fixed urgently in terms of traffic safety. Replacing all of these guardrails with new ones requires a very large cost, but if the guardrails are repaired with a vehicle equipped with the G-Save method, carbon emissions are reduced, the repair period is shortened, and great economic benefits can be obtained. However, risk assessment for guardrail coating vehicles has not been done so far. Focusing on this point, this study aims to evaluate the risk of these coating vehicles and describe the results. Finally, we found that the Risk Priority Numbers(RPN) in the FMECA risk assessment were greatly reduced, and 6 risk factors from HAZOP risk assessment and actions were taken.