• Molecules and Cells
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• v.24 no.2
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• pp.194-199
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• 2007

GCN4 is a typical eukaryotic transcriptional activator that is implicated in the expression of many genes involved in amino acids and purine biosyntheses under stress conditions. It is degraded by 26S proteasomes following ubiquitination. However, the immediate receptor for ubiquitinated Gcn4p has not yet been identified. We investigated whether ubiquitinated Gcn4p binds directly to Rpn10p as the ubiquitinated substrate receptor of the 26S proteasome. We found that the level of Gcn4p increased in cells deleted for Rpn10p but not in cells deleted for RAD23 and DSK2, the other ubiquitinated substrate receptors and, unlike Rpn10p, neither of these proteins recognized ubiquitinated Gcn4p. These results suggest that Rpn10p is the receptor that binds the polyubiquitin chain during ubiquitin-dependent proteolysis of Gcn4p.

• Journal of the Korean Dietetic Association
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• v.21 no.1
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• pp.25-36
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• 2015

In this study, we attempted to improve hospital food delivery service quality and customer satisfaction by using FMEA (Failure Mode and Effect Analysis), which is applied to the quality control of products in manufacturing plants. Subjective food delivery service quality improvement was judged based on a 5-point likert scale. Traditional FMEA uses an RPN (Risk priority number) to evaluate the risk level of a component or process. The RPN index was determined by calculating the product of severity, occurrence, and detection indexes. In our results, total RPN value (P<0.01) significantly decreased after FMEA introduction, whereas customer satisfaction (P<0.001) and food delivery service quality (P<0.001) significantly increased. Specifically, foodservice errors (P<0.01) and loss cost (P<0.01) were significantly improved by FMEA introduction. Taken together, we suggest that FMEA reduces critical activities and errors in foodservice delivery caused by simple priority selection.

• KSBB Journal
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• v.8 no.3
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• pp.217-223
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• 1993

Methods for production of ${\gamma}$-Polyglutamic acid( ${\gamma}$-PGA ) by an alkalophilic Alcaligenes sP. were investigated for batch and continuous culture processes. Both quantity and productivity of ${\gamma}$-PGA by Alcaligenes sp. in batch culture were gradually increased with the increase of glucose concentration up to 50g/l , but the maximal production yield of 63% was obtained at 10g/l of glucose concentration. The highest specific growth rate was about $0.25hr^{-1}$ at 50un of glucose concentration, and substrate inhibition was observed at above 50g1f of glucose concentration. The highest ${\gamma}$-PGA formation about 11g/l in a batch system was obtained at 31'C, pH 10.0 and 87rpn Productivity of 2.80g/l/hr for continuous cultivation was 9 times higher than the productivity for batch cultivation.

• Journal of the Korea Institute of Building Construction
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• v.16 no.6
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• pp.519-527
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• 2016

The demand of construction risk analysis is rapidly increased to improve the competitiveness of construction companies and the sound management of the construction project. However, estimating the amount and uncertainty of the risk is difficult due to the wide range of risks in the construction industry. Moreover, most of the research on risk management of construction risk is only focused on the causes of risk without separate the internal and external risk. This study statistically analysis the internal risk and external risk based on the accidents cases which are caused at construction sites to define the difference and importances of the risk. An accident cause analysis and T-test analysis are carried out to reach the goal of study. The results of the study are expected to be used as a guideline of construction project risk analysis.

• Journal of the Korea Safety Management & Science
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• v.25 no.3
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• pp.73-81
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• 2023

This study uses FMECA (Failure Modes, Effects, and Criticality Analysis) and HAZOP (Hazard and Operability), which are widely applied in industrial areas, among risk assessment methods, and applies them to the same system. While FMECA evaluates system failure conditions and analyzes risks, HAZOP evaluates the system comprehensively by evaluating operational risks that may occur based on system parameters. According to data released by the Ministry of Land, Infrastructure and Transport, as of December 2021, the length of roads in Korea is 113,405 km, and the repair of guardrails that have expired must be fixed urgently in terms of traffic safety. Replacing all of these guardrails with new ones requires a very large cost, but if the guardrails are repaired with a vehicle equipped with the G-Save method, carbon emissions are reduced, the repair period is shortened, and great economic benefits can be obtained. However, risk assessment for guardrail coating vehicles has not been done so far. Focusing on this point, this study aims to evaluate the risk of these coating vehicles and describe the results. Finally, we found that the Risk Priority Numbers(RPN) in the FMECA risk assessment were greatly reduced, and 6 risk factors from HAZOP risk assessment and actions were taken.