• Title/Summary/Keyword: Rot

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Bacillus subtilis YB-70 as a Biocontrol Agent of Fusarium solani causing Plant Root-Rot

  • KIM, YONG-SU;HO-SEONG LIM;SANG-DAL KIM
    • Journal of Microbiology and Biotechnology
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    • v.4 no.1
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    • pp.68-74
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    • 1994
  • A bacterial strain YB-70 which has powerful biocontrol activity against Fusarium solani causing plant root-rot resulting in considerable losses of many economical crops was isolated and selected from over 500 isolates from a ginseng rhizosphere in suppressive soil, and identified as a strain of Bacillus subtilis. In several biochemical and in vitro antibiosis tests on F. solani with culture filterates from B. subtilis YB-70, our data strongly indicated metabolites which mediated inhibition of the fungal growth were presumed to be heat-stable, micromolecular, and ethyl alcohol solutable antifungal substances. Suppression of root-rot by B. subtilis YB-70 was demonstrated in pot trials with eggplant (Solanum melongena L) seedlings. Treatment of the seedling with the bacterial suspension (1.7~1.9$\times$$10^5$ CFU/g) in F. solani-infested soil significantly reduced disease incidences by 68 to 76% after 25 to 30 days. The results supported that B. subtilis YB-70 have excellent potentials as a biocontrol agent.

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the mechanism of Biological Control of Pseudomonas spp.Against Fusarium solani Causing Plant Root-Rot Disease (식물 뿌리썩음병을 유발하는 Fusarium solani에 대한 Pseudomonas 속의 생물학적 방제기작)

  • Lee, Eun-Ju;Kim, Kyung-Seok;Hong, Su-Hyung;Ha, Ji-Hong
    • Microbiology and Biotechnology Letters
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    • v.23 no.1
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    • pp.91-97
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    • 1995
  • The 543 microbial strains were isolated from the rhizosphere in ginseng root rot-suppressive soil. From these, isolate KGM-100 was finally selected for the screening of powerful antagonistic bacterium for the soil-born Fusarium solani causing root rot of many important crops. The isolate KGM-100 was identified as Pseudomonas aeruginosa. Antibiotics produced by the P. aeruginosa KGM-100 were partially characterized as heat-stable and low-molecular weight antibiotics. The strain also produced siderophore, which was assumed to be pseudobactin. Pot test showed approximately 30-50% biological control effects when Fusarium solani-infected soil was treated with the P. aeruginosa KGM-100.

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분리균 Erwinia carotovora subsp. carotovora LY34의 병원성 및 CMCase Isozymes 생성

  • Lim, Sun-Tech;Park, Yong-Yoo;Cho, Soo-Jeong;Yun, Han-Dae
    • Microbiology and Biotechnology Letters
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    • v.25 no.5
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    • pp.468-476
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    • 1997
  • Soft-rot bacterial pathogen, Erwinia sp., was isolated from chinese cabbage tissue showing soft-rot symptom. This bacterial strain caused soft-rot to chinese cabbage and potato, and it was identified as Erwinia carotovora subsp. carotovora LY34 (E. c. subsp. carotovora LY34). Erwinia carotovora subsp. carotovora LY34 did not have hemicellulase but extracellular cellulase, pectinase, polygalacturonase, protease activity. The results of the microscopy showed that chinese cabbage tissue and potato tissue were macerated by infection of E. c. subsp. carotovora LY34. In analysis of the cellulases activity of the isolated cellulose-degradation enzymes from E. c. subsp. carotovora LY34 total protein, three cellulase activity bands were detected by non-denaturation gel electrophoresis method and five cellulase activity bands were detected by CMC-SDS-PAGE direct stain method.

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Identification of Calonectria colhounii Associated with Basal Stem Rot on Blueberry Seedlings Imported from the United States of America

  • Jeon, Nak-Beom;Kim, Wan-Gyu;Park, Myung-Soo;Hyun, Ik-Hwa;Heo, Noh-Youl;Hong, Sung-Kee
    • Mycobiology
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    • v.38 no.4
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    • pp.339-342
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    • 2010
  • Basal stem rot symptoms were found on blueberry seedlings imported from the United States of America in 2008. The fungus obtained from the diseased seedlings was identified as Calonectria colhounii based on morphological and molecular characteristics. The consignments of the blueberry seedlings infected with C. colhounii were destroyed to prevent introduction of the fungus to Korea.

Environmental Factors and Bioremediation of Xenobiotics Using White Rot Fungi

  • Magan, Naresh;Fragoeiro, Silvia;Bastos, Catarina
    • Mycobiology
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    • v.38 no.4
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    • pp.238-248
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    • 2010
  • This review provides background information on the importance of bioremediation approaches. It describes the roles of fungi, specifically white rot fungi, and their extracellular enzymes, laccases, ligninases, and peroxidises, in the degradation of xenobiotic compounds such as single and mixtures of pesticides. We discuss the importance of abiotic factors such as water potential, temperature, and pH stress when considering an environmental screening approach, and examples are provided of the differential effect of white rot fungi on the degradation of single and mixtures of pesticides using fungi such as Trametes versicolor and Phanerochaete chrysosporium. We also explore the formulation and delivery of fungal bioremedial inoculants to terrestrial ecosystems as well as the use of spent mushroom compost as an approach. Future areas for research and potential exploitation of new techniques are also considered.

First Report of Post-Harvest Fruit Rot of Aronia melanocarpa Caused by Fusarium tricinctum in Korea

  • Lee, Hye Won;Nguyen, Thi Thuong Thuong;Lee, Hyang Burm
    • The Korean Journal of Mycology
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    • v.44 no.1
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    • pp.68-71
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    • 2016
  • Black chokeberry, Aronia melanocarpa (Michx.) Elliott, is commonly used as a source of jam and jelly in Korea and worldwide. A fungal isolate EML-CCB6 was isolated from the decaying fruit of black chokeberry. Based on the morphological characteristics and rDNA internal transcribed spacer sequence analysis, the isolate was identified as Fusarium tricinctum (Corda) Sacc. This is the first report of post-harvest fruit rot of black chokeberry caused by F. tricinctum in Korea.

Effect of Soil Fumigation on Growth, Root Rot, and Red Discoloration of Panax ginseng in Replanted Soils (연작지 토양에서 토양연증제 처리가 인삼의 근부, 적변 및 생육에 미치는 영향)

  • 안용준;김홍진
    • Journal of Ginseng Research
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    • v.6 no.1
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    • pp.46-55
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    • 1982
  • The effect of soil fumigation on the growth, yield, root-rot and red discoloration of 2 year-old ginseng, Panax ginseng C. A. Meyer was investigated in the ginseng replanted fie14 Six soil fumigants, Cylone, Basamid, D-D, Dowfume MC-2, Telone C-17, and Vapam were applied in March, 1980, and 2 year-old ginseng plants were transplanted in April, 1981, and sampled in August, 1981. Growth an yields in Cylone and Basamid treated plots in the replanted soil were better than those in control. Especially, growth and yields of ginseng in Cyclone treated Plots were comparable to these in untreated virgin soil. Control effect of soil fumigants used on root-rot was in the order of Cylone, Basamid, Telone C-17, and the lest of them showed neglect fur effect. Frequency of red discoloration was 8% in Cyclone treated plots, 9% in Basamid treated plots 83.3% in the untreated ginseng replanted soil, and 2.5% in the untreated virgin soil.

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Biochemical Study on the Mechanism and Protection of Ginseng Root-Rot Disease (인삼근부병의 기구와 방제에 대한 생화학적 연구)

  • 김병묵;김용환
    • Journal of Ginseng Research
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    • v.6 no.1
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    • pp.75-83
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    • 1982
  • In this study it was found that the enzyme activities of diseased ginseng contributed greatly on the development of its root-rot disease. The 4i sensed ginseng showed higher activities of $\alpha$-amylase, p amylase, invertase, catalase, and cellulase than those of fresh one. The increased enzyme activities of diseased ginseng were originated in those from infected pathogens, which showed a proportional relationship between enzyme activities and root-rot power of them. The increases of enzyme activities during incubation of inoculated ginseng could be considerably depressed by controlling culture environments as to temperature below 4$^{\circ}C$, pH 8-9, and relative humidity about 60%, Some metal ions and organic reagents also inhibited the enzyme activities of diseased ginseng. But their inhibitory effects were not so great that they might be used to protect the disease.

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Effects of Composts and Soil Amendments on Physicochemical Properties of Soils in Relation to Phytophthora Root and Crown Rot of Bell Pepper

  • Kim, Ki-Deok;Nemec, Stan;Musson, George
    • The Plant Pathology Journal
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    • v.16 no.5
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    • pp.283-285
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    • 2000
  • Two field tests were conducted in 1995 to examine the effects of composts and soil amendments on physicochemical properties of soil in relation to Phytophthora root and crown rot of bell pepper. Chitosan, crab shell waste, humate, sewage sludge-yard trimmings, and wood chips were applied to test plots, some of which affected the levels of P, K, Mg, pH, and H. Physicochemical properties were not related with disease incidence, but percent organic matter, estimated nitrogen release, K, and Mg were correlated with total microbial activity. The elements K and Mg were especially responsible for the increased soil microbial activity that could affect development of root and crown rot of pepper.

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A Simple Method for Assessing Severity of Common Root Rot on Barley

  • Arabi, Mohammad Imad Eddin;Jawhar, Mohammad
    • The Plant Pathology Journal
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    • v.29 no.4
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    • pp.451-453
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    • 2013
  • Common root rot caused by Cochliobolus sativus is a serious disease of barley. A simple and reliable method for assessing this disease would enhance our capacity in identifying resistance sources and developing resistant barley cultivars. In searching for such a method, a conidial suspension of C. sativus was dropped onto sterilized elongated subcrown internodes and incubated in sandwich filter paper using polyethylene transparent envelopes. Initial disease symptoms were easily detected after 48h of inoculation. Highly significant correlation coefficients were found in each experiment (A, B and C) between sandwich filter paper and seedling assays, indicating that this testing procedure was reliable. The method presented facilitates a rapid pre-selection under uniform conditions which is of importance from a breeder's point of view.