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http://dx.doi.org/10.5423/PPJ.OA.09.2013.0086

A Simple Method for Assessing Severity of Common Root Rot on Barley  

Arabi, Mohammad Imad Eddin (Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria)
Jawhar, Mohammad (Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria)
Publication Information
The Plant Pathology Journal / v.29, no.4, 2013 , pp. 451-453 More about this Journal
Abstract
Common root rot caused by Cochliobolus sativus is a serious disease of barley. A simple and reliable method for assessing this disease would enhance our capacity in identifying resistance sources and developing resistant barley cultivars. In searching for such a method, a conidial suspension of C. sativus was dropped onto sterilized elongated subcrown internodes and incubated in sandwich filter paper using polyethylene transparent envelopes. Initial disease symptoms were easily detected after 48h of inoculation. Highly significant correlation coefficients were found in each experiment (A, B and C) between sandwich filter paper and seedling assays, indicating that this testing procedure was reliable. The method presented facilitates a rapid pre-selection under uniform conditions which is of importance from a breeder's point of view.
Keywords
barley; Cochliobolus sativus; common root rot; resistance test;
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