• The Journal of the Korean Society for Microbiology
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• v.22 no.2
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• pp.163-166
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• 1987

A new method for rosette assay is described for the detection of antigen-specific lymphocytes from BCG-infected mice using sheep erythrocytes coated with BCG antigen. The optimal concentration of BCG antigen for preparation of indicator cells and the incubation time of antigen coated erythrocytes-lymphocytes mixture were $50\;{\mu}g/ml$ and 1 h, respectively. The number of rosette-forming cells (RFC) during the course of BCG infection showed gradual increase as infection progressed and RFC was reached maximum (about 5-7% of splenic lymphocytes formed rosette) at 3 or 4 weeks after infection.

• Journal of Ecology and Environment
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• v.36 no.3
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• pp.151-158
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• 2013

We estimated phenotypic selection on the height and number of flowering-stalks in a rosette annual Cardamine hirsuta by applying path analysis to the data collected at three natural populations located in central Japan. The path from rosette size was positively connected with the fruit production through the both height and number of flowering-stalks. In the all three populations, the paths from the number of stalks were more strongly connected with the fruit production than from the height of stalks. The paths from the rosette size showed similar magnitude with the number of stalks and the height of stalks. The direct path from rosette size to the fruit production was detected only at one site. These results suggest stronger phenotypic selection on the rosette size through the number of stalks than the height of stalks. The lateral branching rather than increment of individual inflorescence size is the major response to control the fruit production for C. hirsuta growing in a natural habitat.

• Korean Journal of Veterinary Research
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• v.29 no.4
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• pp.525-530
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• 1989

In order to enumerate the T-lymphocytes in bovine peripheral blood lymphocytes (PBL) by E rosette assay, KGRBC were treated with various concentrations of 2-aminoethylisothiouronium bromide(AET) and dextran(Dex), singly or in combination. To further standardize the assay, optimum concentration of AET- and/or Dex-treatment and incubation time for rosette forming cell(RFC) counts were determined. The levels of B-lymphocytes in the PBL were evaluated by erythzocyte-antibody($EA_{Fc}$)- and erythrocyte-antibody-complement (EAC)-rosetting techniques. The results obtained were as follows; The PBL from 20 clinically normal Korean cattle were formed as low percentage of spontaneous E-rosette ($6.7{\pm}2.4%$) in control group, whereas in KGRBC treated with 0.1M AET for 20 minutes and 8% Dex were formed as $37.3{\pm}2.7%$ and $45.1{\pm}2.1%$, respectively. And the synergistic effects were noted no less than $66.5{\pm}5.6%$ when the KGRBC treated with 0.1M AET and 8% Dex subsequently and rate of RFR did not change significantly between 3~24 hours incubation time at $4^{\circ}C$, EA-and EAC-RFR were $23.3{\pm}9.1%$ and $23.1{\pm}7.9%$, respectively. These results suggest that the KGRBC would be a useful agent for the enumeration of T-lymphocytes by E rosette assay and B-lymphocytes by EA- or EAC-rosette assay in cattle-PBL.

• Current Optics and Photonics
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• v.1 no.5
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• pp.491-499
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• 2017

Ghost imaging offers great potential, with respect to standard imaging, for imaging objects in optically harsh or noisy environments. It can solve the problems that are difficult to solve by conventional imaging techniques. Recently, it has become a hot topic in quantum optics. In this paper, we propose a scheme for ghost imaging based on rosette scanning, named rosette ghost imaging. Sampling a small area sampling instead of the whole object, the instantaneous field of view of rosette scanning is used as the modulation light field in ghost imaging. This scheme reduces energy loss, the number of samples, and the sampling time, while improving the quality of the reconstructed image.

• Korean Journal of Veterinary Research
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• v.32 no.2
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• pp.175-179
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• 1992

To develope the methods for isolation and enumeration of lymphocyte subpopulations in pigs, we carried out the rosette-assay using sheep erythrocytes(SRBC) and Korean native goat erythrocytes(GRBC) as a target cells. To enumerate T lymphocytes, E-rosette methods were applied with RBC treated with various concentration of polymers such as Aet and Dex, singly or in combination. And to enumerate B lymphocytes, EAand EAC-rosette assay was adopted. The results were as follows; 1. E-RFR with polymer-untreated SRBC and GRBC was $32.9{\pm}7.9%$ and $31.3{\pm}9.4%$, respectively. On the other hand, RFR with 0.1M Aet plus 8% Dex treated SRBC and GRBC was increased about two-fold($67.8{\pm}7.4%$ and $69.8{\pm}8.5%$), respectively. 2. EA-RFR with SRBC and GRBC were $ 39.1{\pm}10.2%$ and $32.6{\pm}6.1%$, respectively. 3. EAC-RFR with SRBC and GRBC were $27.6{\pm}7.0%$ arld $21.0{\pm}3.2%$, respectively. These results showed that both SRBC and GRBC could be recommanded as a binding cells for rosetteassay to isolate of lymphocyte-subpopulations in pigs.

• Journal of The Korean Astronomical Society
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• v.3 no.1
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• pp.7-11
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• 1970

Optical thickness of Rosette Nebula for Lyman Continuum is examined with W. Gebel's data. And the effective temperatures of the six exciting 0 stars of the nebula are obtained on the assumption that O stars emit black body radiation. The results are found to be generally in between Spitzers old and revised values.

• Korean Journal of Veterinary Research
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• v.26 no.1
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• pp.117-124
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• 1986

The present study was undertaken to evaluate rosette formation and NK activity of splenic lymphocytes in 3-methylcholanthrene (MCA) treated mice. Mice were sensitized iv with 0.1ml of 1% sheep red blood cell (SRBC) suspension were treated with a single ip injection of olive oil alone or with different doses of MCA in oil at various time before or after sensitization, and were challenged at 4 days after SRBC. Rosette formation and NK activity of splenic lymphocytes were measured at 24 hours after challenge. Erythrocyte(E) rosette formation of splenic lymphocytes was significantly depressed in mouse treated with large dose of MCA (5~50mg) regardless of injecting time. But, there was no difference in the response between the treated with small dose of MCA (0.5mg). Whereas erythrocyte-antibody(EA) rosette or erythrocyte-antibody-complement(EAC) rosette forming cells were significantly depressed by MCA. Under small dose of MCA (0.5mg), any difference of NK activity was not observed in all course of injecting time. But, under large dose of MCA, the activity was markedly inhibited to about half the values seen in control and this suppression was transient, resulting that the normal level was reached again 19 days after MCA. These results, which conform with the predictions of immunosuppression hypothesis, suggest that MCA inhibits immunological responses including NK activity and thereby allows the outgrowth of antigenic neoplastic cells.

• Steel and Composite Structures
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• v.1 no.1
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• pp.17-32
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• 2001

The Rosette-joining system is a completely new press-joining method for cold-formed steel structures. One Rosette-joint has a shear capacity equal to that of approximately four screws or rivets. The Rosette thin-walled steel truss system presents a new fully integrated prefabricated alternative to light-weight roof truss structures. The trusses are built up on special industrial production lines from modified top hat sections used as top and bottom chords and channel sections used as webs which are joined together with the Rosette press-joining technique to form a completed structure easy to transport and install. A single web section is used when sufficient but can be strengthened by double-nesting two separate sections or by using two lateral profiles where greater compressive axial forces are met. An individual joint in the truss can be strengthened by introducing a hollow bolt into the joint hole. The bolt gives the connection capacity a boost of approximately 20%. A series of laboratory tests have been carried out in order to verify the Rosette truss system in practice. In addition to compression tests on individual sections of different lengths, tests have also been done on small structural assemblies and on actual full-scale trusses of a span of 10 metres. Design calculations have been performed on selected roof truss geometries based on the test results, FE-analysis and on the Eurocode 3 and U.S.(AISI) design codes.

• Journal of Korean Society of Water and Wastewater
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• v.22 no.3
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• pp.389-396
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• 2008

The hybrid model can be used to predict the initial near field mixing and the far field transport of the buoyant jets, which are discharged from the submerged wastewater ocean outfall. In the near field, the jet integral model can be used for single port diffusers while the ${\sigma}$ transformed particle tracking model was used in the far field. In this study, the experimental study was performed to verify the developed hybrid model in the previous research. The developed hybrid model properly predict the surface and vertical concentration distribution of the single buoyant jets with various effluent and ambient conditions. The hybrid model can also simulate the surface concentration distribution of the rosette diffuser except for the parallel diffuser with the higher densimetric Froude number due to the assumption that dynamic effects of the effluent plumes are negligible in the far field. The application of the hybrid model to rosette diffusers can predict the concentration near the diffuser more accurately when the line-plume approximation is used.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.35S no.7
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• pp.86-94
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• 1998

The rosette-scan seeker is a device mounted on the infrared guided missile. It offers the positions and iamges of target to missiles servo system by scanning a space about target in rosette pattern with a single detector. An instantaneous field of view (IFOV), which is a diameter of a detector moving along the path of the rosette pattern, has the property that its smaller size provide the less interference of background signals and detector noise. If its size is too small to voer the total field of view (TFOV), however, it produces the invisible regions in the TFOV. In this case, the invisible regions cause the performance of the seeker to deteriorate. For full scan-coverage, it is necessary to design the small IFOV without the invisible regions in the TFOV, as possible. In this paper, we propose the new method of designing the smaller IFOV than the conventional method and verify full coverage of the scanned region. By comparing the nose equivalent flux density (NEFD) of the proposed method with the that of the conventional one, we confirm that the former is better than the latter in terms of performance.