• Title/Summary/Keyword: Rosetta

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3D Structure of STAM1 UIM-ubiquitin Complex Using RosettaDock

  • Lim, Jong-Soo;Yi, Jong-Jae;Ahn, Hee-Chul;Rhee, Jin-Kyu;Son, Woo-Sung
    • Journal of the Korean Magnetic Resonance Society
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    • v.15 no.1
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    • pp.80-89
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    • 2011
  • 3D structures of STAM1 UIM-ubiquitin complex were presented to predict and analyze the interaction between UIM and ubiquitin. To generate the protein-peptide complex structure, the RosettaDock method was used with and without NMR restraints. High resolution complex structure was acquired successfully and evaluated electrostatic interaction in the protein-peptide binding with several charged residues at the binding site. From docking results, the Rosettadock method could be useful to acquire essential information of protein-protein or protein-peptide interaction with minimal biological evidences.

Overview of Rosetta for Estimation of Soil Hydraulic Parameters using Support Vector Machines (보조벡터기로를 사용한 토양수리계수 추정을 위한 로제타 개관)

  • Chung, Doug-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.spc
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    • pp.8-13
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    • 2009
  • Mathematical models have become increasingly popular in both research and management problems involving flow and transport processes in the subsurface. Rosetta is a program to estimate unsaturated hydraulic properties from surrogate soil data such as soil texture data and bulk density. Models of this type are called pedotransfer functions (PTFs) as an alternative measurements since they translate basic soil data into hydraulic properties. These functions may be either measured directly or estimated indirectly through prediction from more easily measured data based using quasi-empirical models.

Adhesion between heat-pressed lithium disilicate veneer and zirconia framework: Shear bond strength evaluation (열가압 리튬 디실리케이트 전장도재와 지르코니아 하부구조의 전단결합강도 평가)

  • Kim, Jae-Hong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.3
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    • pp.335-341
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    • 2020
  • This study examined the shear bond strength between the zirconia core and pressed lithium disilicate veneering ceramics. The Schmitz-Schulmeyer test method was used to investigate the core-veneer shear bond strength of industrially manufactured zirconia core ceramic (Zirtooth, HASS, Gangneung, Korea) and pressed veneer ceramic (IPS e.max Zirpress, Vita PM9, GC Initial IQ, HASS Rosetta SM) (N=40). Data were statistically analyzed using one-way ANOVA and Tukey's test (a=0.05). The fractured surfaces of the specimens were examined to determine the failure pattern using a digital microscope. The mean ± SD shear bond strength in MPa were 16.69±3.11, 14.21±3.63, 11.17±2.92, and 27.90±5.71 for IPS e.max Zirpress, VITA PM9, GC Initial IQ, and HASS Rosetta SM, respectively. The average shear bond strength was largest for HASS Rosetta SM, followed by IPS e.max Zirpress, Vita PM9, and GC Initial IQ(p<0.05). The digital microscopy examination of the fracture surface showed adhesive and cohesive failure in pressed lithium disilicate veneering ceramics. The use of lithium disilicate veneer ceramic produced a significantly higher shear bond strength.

Enhanced Heterologous Expression of Aspergillus niger Epoxide Hydrolase and Its Application to Enantioselective Hydrolysis of Racemic Epoxides (Aspergillus niger의 Epoxide Hydrolase 고효율 발현 및 라세믹 에폭사이드의 입체선택적 가수분해)

  • Lee, Soo Jung;Kim, Hee Sook;Lee, Eun Yeol
    • Applied Chemistry for Engineering
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    • v.17 no.5
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    • pp.557-560
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    • 2006
  • The epoxide hydrolase (EH) of Aspergillus niger LK was expressed to high levels in Escherichia coli based on codon usage. E. coli, Rosetta (DE3)PLysS, containing a large number of tRNAs for rare-codons was employed as a host strain. The recombinant E. coli expressing A. niger EH showed an enhanced enantioselective hydrolysis activity toward racemic styrene oxide. Enantiopure (S)-styrene oxide with a high enantiopurity of 99% ee was obtained from racemic substrates.

A Method of XML Mapping Canonicalization for E-Business Integration (전자상거래 통합을 위한 XML 매핑 정형화 기법)

  • 안우영;홍창범
    • Journal of the Korea Society of Computer and Information
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    • v.9 no.1
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    • pp.1-8
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    • 2004
  • XML is becoming the standard of the new document exchanging. Due to the ablility expressing various types of document structure through XML, RosettaNet and BizTalk are using XML as a core technology in the part of e-Business. Framework is running Business process each other different standard. Internal documents in each company should be transformed differently without any loss to work with other companies. In this paper, transforming Processor based on XML mapping information from XML document information.

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E-Transformation from EDI to Web-based B2B Frameworks

  • Kim, Minsoo;Ock, YoungSeok;Kim, Dongsoo
    • Industrial Engineering and Management Systems
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    • v.7 no.2
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    • pp.150-159
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    • 2008
  • Lots of EDI-VAN companies are in need of transforming their business transaction systems into Web-based e-Business frameworks because of high cost and closed structure of EDI systems. This research proposes several e-Transformation strategies for EDI-VAN companies to adopt Web-based e-Business frameworks such as ebXML and RosettaNet. Four transformation alternatives are presented and transformation procedure for a medium-sized company is described. The result of this work can be used as a practical guideline for EDI companies to develop their own transformation strategy suitable to its scale and capability, while minimizing the impacts on the pre-existing business processes and information systems.

Development of Recombinant Escherichia coli Expressing Rhodotorula glutinis Epoxide Hydrolase (Rhodotorula glutinis의 epoxide hydrolase 고효율 발현 유전자 재조합 Escherichia coli 생촉매 개발)

  • Lee Soo-Jung;Kim Hee-Sook
    • Journal of Life Science
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    • v.16 no.3 s.76
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    • pp.415-419
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    • 2006
  • The epoxide hydrolase (EH) of Rhodotorula glutinis which has a high enantioselectivity against aromatic epoxide substrates was expressed to high levels in Escherichia coli based on codon usage. We analysed the Preference of codon usage between the yeast, R. glutinis, and bacteria, E. coli. E. coli, Rosetta(DE3)pLysS, harbors pRARE plasmid with tRNA genes for rare-codons was employed as a host strain. The recombinant E. coli expressing R. glutinis EH showed an enhanced enantioselective hydrolysis activity toward racemic styrene oxide. Enantiopure (S)-styrene oxide with a high enantiopurity of 99% ee (enantiomeric excess) was obtained from racemic substrates.

RosettaNet based Business Integration Model for Enhancing Manufacturing Firm's Responsiveness (제조 기업의 대응성 향상을 위한 로제타넷 기반 비즈니스 통합 모델)

  • Park, Mok-Min;Park, Jeong-Ho;Shin, Ki-Tae;Park, Jin-Woo
    • The Journal of Society for e-Business Studies
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    • v.15 no.1
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    • pp.89-101
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    • 2010
  • In some industries, manufacturing firms' distinctive competences include not only product superiority but also service excellence. For example, in a highly volatile and dynamic market, flexibility and responsiveness are more valued than just the product cost. And sales order processing, in that respect is a very important process. However, previous studies on sale order processing have considered only due-date assignments or dealt with production scheduling to minimize production cost or delivery lead time. Due to recent advances in information technology, a new approach is possible in dealing with sales order processing. For example, RFID(Radio Frequency IDentification) and ubiquitous computing technology adds real time visibility and traceability to supply chain. In this study, we develop a new due-date re-negotiation process for sales order management and propose a new business integration model to support the re-negotiation process based on RFID, ubiquitous computing technology and RosettaNet's PIPs.