• Title/Summary/Keyword: Rooting

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Effect of Plant Growth Regulators on Callus Induction and Plant Regeration of Farfugium japonica (털머위 (Farfugium japonica)의 캘러스 유도 및 식물체 분화에 미치는 생장조절제의 영향)

  • Lee, Seung-Yeob;Yoo, Sung-Oh;Bae, Jong-Hyang;Lee, Joong-Ho
    • Journal of Plant Biotechnology
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    • v.29 no.1
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    • pp.45-49
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    • 2002
  • The leaf and petiole segments of Farfugium japonica were cultured to investigate the influence of growth regulators on their callus induction and plant regeneration. The callus induction and growth showed a good response both leaf and petiole on MS media supplemented with 1∼2 mg/L 2,4-D and 1∼2 mg/L BA. Callus induction and growth were more effective in petiole segments than leaf one. The highest percentage of plant regeneration was obtained from 60-day-old calli on MS medium supplemented with 1 mg/L NAA and 2 mg/L BA. When subcultured to the same medium for about 60 days, multiple shoots were developed from regenerating callus. The shoots produced roots after transferring to rooting medium containing 0.5 mg/L IAA. The plantlets over 50 mm in height were successfully acclimatized in vermiculite, and the survival rate was over 95%.

Studies on the Preservation of Korean Chestnnt by Gamma Irradiation -Part 1. Radiation Effect of Low Oxygen Atmosphere- (방사선조사(放射線照査)에 의한 밤저장연구(貯藏硏究) -제1보 저산소상태(低酸素狀態)에서 방사선조사효과(放射線調査効果)-)

  • Park, Nou-Poung;Kim, Yun-Jin;Kim, Sung-Kih;Rhee, Chung-Ouk
    • Korean Journal of Food Science and Technology
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    • v.9 no.1
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    • pp.36-40
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    • 1977
  • Experiments were carried out to determine the optimum dose for the preservability of each Korean chestnut varieties by means of gamma-radiation. They were associated with control of spots in the edible portion of chestnut due to irradiation at the final stage of storage. With this result the following summary can be made. 1. In consideration of those factors such as rooting, sprouting, rotting and undesirable spots on the flesh, it was shown that the optimum radiation dose was checked to be 25-35 Krad though there are some difference among the varieties of chestnut. 2. Rotting and the spots on the flesh of chestnut were remarkably inhibited by low oxygen atmosphere during irradiation. This condition greatly contributed to inhibit sprouting with the low radiation dose(15 Krad). 3. The nuts irradiated had a marked increase in carbon dioxide evolution immediately after the irradiation. However on the day succeding the treatment, these increased curves gradually normalized as same as that of untreated.

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Effect of Various Sources of Fertilizers and Their Application Methods on Seedling Vigor in Rice for Machine Transplanting (비료(肥料)의 종류(種類)와 시비법(施肥法)이 상자육묘(箱子育苗)에 있어서 묘생육(苗生育)에 미치는 영향(影響))

  • Jeh, Sang Yull;Pae, Suk Bok
    • Current Research on Agriculture and Life Sciences
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    • v.2
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    • pp.1-8
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    • 1984
  • This study was conducted to evaluate the effect of various fertilizers and their application methods on pH in seedbed soil and seedling vigor of rice cultivars, "Nagdong" and" Samgang", for machine transplanting. The application of amnonium sulfate as nitrogen sources showed higher plant height and dry weight of seedlings than those of the urea treated plot. Seedling rot was highly occured in the basal application of urea than that of split treatment of urea, while the lower seedling rot was observed in ammonium sulfate treated plot than that of treatment. Regardless of the application methods, the higher rooting ability was observed in anmonium sulfate treatment than that of urea. Ammonium sulfate and superphosphate as nitrogen and phosphate sources, respectively, showed lower pH level than that of urea and fused phosphate treated plots. The use of ammonium sulfate and super phosphate as nitrogen and phosphate sources, respectively, seems to be effective to maintain the optimum pH level and to rear the healthy seedling, than that of urea or fused phosphate application.

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Studies on the Dormancy of Liatris spicata Corm -I. Study on the Induction and Awakening of Dormancy and Effect of Gibberellin Treatment on the Breaking of Dormancy (Liatris Spicata 구경휴면(球莖休眠)에 관(關)한 연구(硏究) -I. 휴면도입기(休眠導入期) 및 각성기(覺醒期) 구명(究明)과 gibberellin 처리(処理)의 휴면타파효과(休眠打破效果))

  • Park, In Hwan;Suh, Young Kyo;Chun, Chae Ki;Choi, Sang Tai
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.18-21
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    • 1986
  • This study was conducted to clarify the period of induction and awakening of Liatris spicata corm dormancy, and investigated effect of gibberellin treatment on the dormancy break of corm. The results were obtained as follows ; 1. Ratio of sprouting, shooting and rooting were gradually declined after August 30. These ratio showed the lowest to trend on November 14 during experiment and gradually increased after this time. Ratio were excellent as 100% after January 29. 2. Also, average days for sprouting were the longest on the November 14, but it was rapidly declined after January 29. 3. The period of induction and awakening of corm dormancy were late in September and from late January to early February, respectively, in Daegu, Korea. 4. Effects of GA-treatment on the dormancy break of corm were excellent. Especially, its effects on the dormancy break were the deeper in degree of dormancy, the better.

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The Cuttings of the Genus Lespedeza (싸리류(類)의 삽목시험(揷木試驗))

  • Han, Young Chang
    • Journal of Korean Society of Forest Science
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    • v.20 no.1
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    • pp.45-50
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    • 1973
  • This study was investigated the methods of vegetative propagation for new Lespedeza varieties. Experimental matterials were used 6 Lespedeza species; L. maximowiczii, L. cyrtobotrya, L. angustifolioides, L. bicolor, L. maximowiczii var. tomentella, and L. japonica var. intermedia. In April a year branches were cut 15-20cm length and 3-4mm thickness and planted in sand bed (depth 150cm) to be sterilized by the 0.1% solution of Uspulun. And then the cutting beds were irrigated and shaded. The results are as follows. 1. The rooting rates of the cuttings could be found so variable among varieties from 69% of L. maximowiczii var. tomentella to 50.3% of L. japonica var. intermedia. 2. The growth performance showed statistically significant difference at 1% level among varieties. L. maximowiczii was the best, while L. japonica var. intermedia showed the wrost growth performance of them. 3. The average number of roots with more than 5 cm length per stock was revealed statistically significant difference at the 1% level among varieties from the greatest number of 6.4 of L. maximowiczii var. tomentella to the smallest number of 2.3 of L. japonica var. intermedia. 4. Total average length of the roots per stock was statistically significant so different at 1% level from the longest 279.8cm of L. maximowiczii var. tomentella to the shortest 41.1cm of L. japonica var. intermedia. 5. The average ndules with more than 1 mm in diameter per stock was statistically significant so different at 1% level among varieties from the greatest number of 34.4 of L. maximowcizii to the smallest numqer of 4.6 of L. japonica var. intermedia.

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In Vitro Plantlet Regeneration from Axillary Buds of Tilia amurensis Mature Trees and Clonal Variation in Tissue Culturability (피나무 성숙목(成熟木)의 액아배양(腋芽培養)에 의한 유식물체(幼植物體) 재생(再生)과 조직배양능력(組織培養能力)에 있어서의 클론간(間) 변이(變異))

  • Youn, Yang;Ohba, Kihachiro
    • Journal of Korean Society of Forest Science
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    • v.79 no.2
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    • pp.109-114
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    • 1990
  • The axillary buds of 15-year-old Tilia amurensis were cultured on Saito and Ide (IS), Murashige and Skoog (MS) media and woody plant medium (WPM) to establish an effective micropropagation method. Five levels of 6-benzylaminopurine (BAP) were tested. On IS medium and WPM addition of 1.0/l BAP enhanced shoot development and shoot elongation, whereas addition of 0.5/l BAP was effective on MS medium. A better results were obtained from WPM with 1.0/l BAP and MS with 0.1/l BAP. Developed shoots were subcultured on each basal media but with 0.2/l BAP, Multiple shoots were almost doubled in a month. Root formation could be enhanced at higher concentration of indole-3-butyric acid (IBA). Better rooting rate (83.3%) was achieved on a half-strength MS medium with 3.0 /l IBA. Regenerated plantlets were successfully transferred to soil. To investigate the clonal variation in shoot development and shoot elongation by axillary bud culturing, seven plus tree clones were tested, Clonal variation in tissue culturability among plus trees was recognized by the Duncan's multiple range test at the 5% level. Kang Won No. 12 showed the best response on WPM with 1.0/l BAP.

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Growth Characteristics of Angelica gigas Nakai on Transplantation Season (April and Autumn) (정식계절(봄, 가을)에 따른 참당귀(Angelica gigas Nakai)의 생장특성)

  • Jeong, Dae Hui;Kim, Nam Soo;Kim, Ki Yoon;Park, Hong Woo;Jung, Chung Ryul;Kim, Hyun-Jun;Jeon, Kwon Seok;Kim, Mahn Jo
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.72-72
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    • 2019
  • This study compared the growth characteristics of A. gigas according to the transplanting season, in the cultivation located in Yeongju, Gyeongsangbuk-do, transplanted in the autumn and spring. As a result of that the plant survival rate were observed the highest autumn transplanting (9.4%; autumn-92.2%, spring-82.8%) and bolting rate were observed lowest spring transplanting (7.1%; autumn-37.3%, spring-30.2%). Growth characteristics (height, leaf length and width, stem diameter) were observed the highest autumn transplanting in June and highest spring transplanting in August. The early growth is high growth due to long rooting time in autumn transplanting, but the difference in the ground growth between the two experiments was insignificant when the main growth period was from June to August. Further analysis of the growth characteristics and marker components of roots of A. gigas can be used to determine the optimal planting time and the establishment of high quality cultivation technology.

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Interpreting Soil Tests for Turfgrass (잔디 토양 분석의 해석)

  • Christians, Nick;Joo, Young-Kyoo;Lee, Jeong-Ho
    • Asian Journal of Turfgrass Science
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    • v.20 no.2
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    • pp.223-235
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    • 2006
  • Soil testing laboratories unfamiliar with turfgrasses will often overestimate the plant's need for phosphorus and underestimate the need for potassium. This is partly due to differences in rooting between grasses and many garden plants and crops. The grasses are generally more efficient in extracting phosphorus from the soil, reducing their need for phosphorus fertilizer. The fact that crop yield is often the primary objective in field crop production, and is usually of little interest in turfgrass management, may affect soil test interpretation for potassium. Potassium levels above those required for maximum tissue yield of grasses may improve stress tolerance and turfgrasses will usually benefit from higher applications of this element. There are also diffrrences in soil testing philosophies. Some laboratories use the sufficiency level of available nutrients(SLAN) approach, whereas others prefer the basic cation saturation ratio(BCSR) approach. Some will use a combination of the two methods. The use of the BCSR theory easily lends itself to abuse and questionable fertilizer applications and products are sometimes recommended citing imbalances in cation ratios. The usefulness of the BCSR ratio theory of soil testing varies with soil texture and interpretations on tests performed on sand-based media are particularly a problem. Other soil testing problems occur when sand-based media used on sports fields and golf greens contain free calcium carbonate. The ammonium acetate extractant at pH 7.0 dissolves excessive amounts of calcium that can bias cation exchange capacity measurements and measurements of cation ratios. Adjusting the pH of the extractant to 8.1 can improve the accuracy of the testing procedure for calcareous media.

Postharvest Handling and Marketing Management for Making High Salability of Sweetpotatoes (상품성 제고를 위한 고구마 수확 후 관리 및 출하기술)

  • Jeong, Byeong-Choon
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2001.06a
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    • pp.51-64
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    • 2001
  • The qualities including taste of sweetpotato stored during the winter which can display in the spring market in Korea are affected by availability of storage for the roots. In order to make high storage availability of sweetpotato, the postharvest handlings should be done thoroughly from the moment of harvest until shipping them to the market. A lot of procedures that must be handled carefully for improving postharvest management are as follows; digging, trimming, gathering, putting in storage containers, carrying them from field to house, curing, storing, washing, drying, selecting marketable roots, packing and shipping to the market, etc.. Sweetpotatoes have a high moisture content, and a relatively thin and delicate skin, and are sensitive to chilling, so careless postharvest handling can lead to both quantitative and qualitative losses which may be extremely high in some circumstances. From now on research has concentrated on the improvement of postharvest conditions to increase yield and lower disease rates. Storage, which makes sweetpotatoes available through out the year, benefits both the producer and the consumer. Seven very important points must be needed in order to get the best quality marketable roots in the storing of sweetpotatos : $\circled1$The storage house must be clean and sanitary, $\circled2$The crop must be harvested before the first frost to avoid low-temperature injury, $\circled3$Particular care must be taken to avoid cutting, bruising, or other injuries of the sweetpotatoes during digging, picking up, grading, placing in containers, and moving to the storage house, $\circled4$Select sound, disease-free roots for storage $\circled5$Sweetpotatoes should be stored in properly stacked containers $\circled6$Cure immediately after harvest, preferably at 32∼33$^{\circ}C$ and 90 to 95 percent relative humidity for 4 to 7 days, After curing the temperature should be reduced to 13$^{\circ}C$ to 16$^{\circ}C$ by ventilating the storage with outside air. $\circled7$Store at 12$^{\circ}C$ to 14$^{\circ}C$ and a relative humidity of 80 to 85 percent. Storage houses should be located on suitable sites and should be tightly constructed and insulated so that temperature and humidity will be uniform. Sweetpotatoes are usually not washed and graded, and lately sometimes washed, graded, waxed, before being shipped to market. Consumer packaging of sweetpotatoes in paper boxes(10-15kg) or film bags is done mainly to aid marketing. The shelf life of washed roots in consumer packs in only 1 to 2 weeks. Weight loss of roots during marketing is much less in perforated film bags than in mesh and paper bags. Perforation of 0.8 to 1kg polyethylene bags with about six 6mm holes is essential ; to lower the internal relative humidity and avoid excessive sprouting, rooting, and dampness. Development and use of better postharvest handling with good storage facilities or marketing methods can minimize sweetpotate losses and has an effect of indirectly increasing productivity and farmer’s income.

Effect of growth regulators on In Vitro mass propagation of Haworthia maughanii (생장조절제가 하월시아 만상(Haworthia maughanii)의 기내 대량증식에 미치는 영향)

  • Kim, Youn Hee;Kim, Hye Hyeong;Lee, Gee Young;Lee, Jae Hong;Jung, Jae Hong;Delgado-Sanchez, Pablo;Lee, Sang Deok
    • Journal of Plant Biotechnology
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    • v.45 no.4
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    • pp.369-374
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    • 2018
  • The purpose of this study was to investigate the suitable parts for callus induction and optimal concentrations of growth regulators contained in the medium affecting shoot and rooting for the in vitro mass production of Haworthia maughanii. To determine suitable parts of the plant for callus induction, the leaves, flower bloom and flower stalks were cultured in MS medium at different concentrations of $0{\sim}2mgL^{-1}$ NAA and $0{\sim}2mgL^{-1}$ TDZ, respectively. All of the parts showed 100% callus formation rate at $NAA\;1mgL^{-1}$ and $TDZ\;1mgL^{-1}$ treatment, $NAA\;2mgL^{-1}$ and $TDZ\;2mgL^{-1}$ treatment and NAA 1 to $2mgL^{-1}$, respectively. While the rate of callus formation was high in all parts, the leaves were the most efficient to obtain most culture parts. $NAA\;0.1mg\;L^{-1}$ and $BA\;0.1mg\;L^{-1}$ treatments were the most effective in shoot formation with 22.0 shoots. In addition, multiple shoot propagation showed 16.3 shoots, the highest, with $NAA\;0.1mg\;L^{-1}$ and $BA\;0.1mg\;L^{-1}$ treatments. These results led us to speculate that the optimization of culture conditions was responsible for the mass propagation for in vitro cultures of Haworthia maughanii.