• Journal of agriculture & life science
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• v.43 no.4
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• pp.33-36
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• 2009

Globose to clavate base-ball sized, pear shaped, fruiting bodies were found under the Himalayan cedar, Cedrus deodora at less fertile and poor sandy poor soil in the campus of Gyeongsang National University in Jinju, Korea. The fruiting body was at first, round to club-shaped, usually with a narrow, rooting base with yellowish rhizomorphs attached to it and lack a volva and a sterile base. The peridium of fruiting body was tough and crusty. The peridioles were white pea-like capsules in a blackish matrix. The color change to darker tints of brown at the top of the exterior peridium reflected the gradual ripening of the interior gleba and peridioles, which proceeded from the top downward to become a mass of spore dust, appearing as cinnamon brown at the apex of the vertical section. At around this stage, the peridium cracked open linearly, exposing the gleba with powdery spores mass released from overmatured peridioles. Spores were more or less round, warty or spiny, 10 to $12{\mu}m$; globose, cinnamon brown in powdery mass, with spines up to $2{\mu}m$ long. The thin peridium ruptured further in response to the disintegration of the peridioles, releasing the powdery spores, which proceeded until whole fruiting body disappeared leaving the dry spore dust coats in the vicinity. The absence of a capillitium is a distinctive characteristic that distinguishes the specimen from other puff-ball fungi and from most of earthballs. Based on the above characteristics, the specimen was identified as Poslithus tinctorius.

• Asian Journal of Turfgrass Science
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• v.7 no.1
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• pp.19-30
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• 1993

Experiments were conducted to investigate propagation, shade tolerance, cold resistance and growth rate of evergreen ground cover plants ; Vinca minor K , Ajuga reptans L., Ophiopogon japonicus ker. f nanus hort , and Hedera helix.. The results were as follows : 1.It is convenient to use the growing case for rice seed to grow and transplant Vinca minor seed-ling. The most proper density of transplanting Vinea was 180 plants per 1m$^2$. At the end of growing season, the coverage came up to 90% The rooting ability of Hedera helix was best to cut from April to May (temperature 15~23˚C). The seedling length of Parthenocissus quinguefohlia were irregular and ranged from 5 cm of 200cm. 2. Ajuga reptans L., Ophiopogon j. and Vinca minor L. grew better under 50% light intensity than full sunlight. Particularly, under full sunlight and aestival high temperature, there were a few withering plants in Ajuga reptans L. , and V Vilica minor L. 3. Of Vinca minor K., Ajuga reptans L. , Ophiopogon japonicus ker. f. nanus hort. and Hedera helix. acclimatized in open field, where temperature was from -7~8˚C. There were few plants had damage in low temperature incubator to -16˚C after field acclimatization. 4.Tendrils of Parthenocissus quinguefolia strongly clung to the wire netting stone fence but intruded into the gap and cranny of the precast concrete fence and so clung to, so that it needed to install the bending net on the precast concrete fence.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.5
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• pp.564-569
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• 2015

In highland crop fields, saprolite is piled up approximately every three years as deep much as 20 to 30 cm because farmers expect that adding new materials may improve productivity and mitigate hazards by continuous cultivation of a single crop. Piling saprolite, however, has been reported to induce poor soil drainage. Effects of deep ploughing with a spading machine and an excavator were studied in sites located in Daekwanryeong-myeon, Pyeongchang in which soil physical properties were deteriorated by piled saprolite. The soil made of parent material of Samgag series was piled up over surface soil of Haggog series naturally developed in the area. Carrot was cultivated in the field. Productivity and growth factors of carrot were compared among control and deep ploughing by a spading machine and an excavator. Effective soil depth extended to 60 cm or greater by 60 cm deep ploughing by an excavator or 50 cm deep ploughing by a spading machine. On the other hand, effective soil depth was within 50 cm at control plot. Productivity of carrot responded to amelioration of soil physical properties. The productivity was greater in deep ploughing treatments than that of control or 30 cm ploughing. It suggested that increased productivity by deep ploughing was mainly related to breaking plough pan which inhibited extension of rooting zone.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.6
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• pp.1010-1015
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• 2011

The objectives of this study were to investigate soil chemical characteristics for forest rehabilitation and suggest management in abandoned coal mine areas in Hwasun-gun, South Jeolla Province. Total study sites were 8 sites, and soil analysis particular were soil pH, TOC, total-N, C/N ratio, Avail. $P_2O_5$, and CEC. Average soil pH was 5.8 (4.7~6.4). Average contents of TOC, total-N and C/N ratio were 1.1% (0.2~2.0%), 0.08% (0.02~0.13%) and 15.0 (7.9~31.4), respectively. Average Avail. $P_2O_5$ was $8.3mg\;kg^{-1}$ (2.7~15.0) and Average CEC was $13.7cmol_c\;kg^{-1}$ ($9.9{\sim}18.5cmol_c\;kg^{-1}$). Soil pH was decreased according to elapsed time from forest rehabilitation, while TOC, total N and CEC were increased. Av. $P_2O_5$ did not show any relationship with elapsed time. Soil pH was stable comparing with general forest soil in South Jeolla Province (5.1), while contents of TOC and total N were lower than general forest soil in South Jeolla Province (4.9% and 0.18%, respectively). Therefore, sustainable managements such as fertilization for TOC and total N are necessary for good rooting and growth of vegetation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.3
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• pp.213-224
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• 2019

There is an increasing frequency in the occurrence of abnormal weather phenomena such as sharp increases and decreases in temperature. Under these weather conditions, the heading time of rice changes unexpectedly, which poses problems in agriculture. Therefore, we investigated the effect of temperature on the heading response at different growth stages in rice. During the period from transplanting to heading, the plants were subjected to different temperature treatments, each for a 9-day period, to observe the heading response. For the heading date analysis, "heading date" was defined as the number of days from transplanting to the appearance of the first spikelet. We found that the influence of temperature increased in the order of rooting stage, followed by meiosis, early tillering, spikelet differentiation, and panicle initiation stage in all ecological types and cultivars. In particular, unlike the results reported previously, the effect of temperature on heading during the photo-sensitive period was very small. Meanwhile, the influence of temperature on vegetative growth response at different growth stages was not consistent with heading response. These results can be used as basic data for predicting the variation in heading date owing to temperature variation at each growth stage. In addition, we propose that the concept of day length should be included in determining the influence of temperature on the photo-sensitive period.

• Journal of Animal Science and Technology
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• v.63 no.2
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• pp.248-261
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• 2021

Attributable to their major function in pathogen recognition, the use of bovine leukocyte antigens (BoLA) as disease markers in immunological traits in cattle is well established. However, limited report exists on polymorphism of the BoLA gene in zebu cattle breeds by high resolution typing methods. Thus, we used a polymerase chain reaction sequence-based typing (PCR-SBT) method to sequence exon 2 of the BoLA class II DRB3 gene from 100 animals (Boran, n = 13; Sheko, n = 20; Fogera, n = 16; Horro, n = 19), Hanwoo cattle (n = 18) and Bangladesh Red Chittagong zebu (n = 14). Out of the 59 detected alleles, 43 were already deposited under the Immuno Polymorphism Database for major histocompatibility complex (IPD-MHC) while 16 were unique to this study. Assessment of the level of genetic variability at the population and sequence levels with genetic distance in the breeds considered in this study showed that Zebu breeds had a gene diversity score greater than 0.752, nucleotide diversity score greater than 0.152, and mean number of pairwise differences higher than 14, being very comparable to those investigated for other cattle breeds. Regarding neutrality tests analyzed, we investigated that all the breeds except Hanwoo had an excess number of alleles and could be expected from a recent population expansion or genetic hitchhiking. Howbeit, the observed heterozygosity was not significantly (p < 0.05) higher than the expected heterozygosity. The Hardy Weinberg equilibrium (HWE) analysis revealed non-significant excess of heterozygote animals, indicative of plausible over-dominant selection. The pairwise FST values suggested a low genetic variation among all the breeds (FST = 0.056; p < 0.05), besides the rooting from the evolutionary or domestication history of the cattle. No detached clade was observed in the evolutionary divergence study of the BoLA-DRB3 gene, inferred from the phylogenetic tree based on the maximum likelihood model. The investigation herein indicated the clear differences in BoLA-DRB3 gene variability between African and Asian cattle breeds.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.873-879
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• 2020

The importance of data as a core resource of the 4th industrial revolution is emerging, and companies illegally collect and use personal data. In the financial sector, active research is conducted to safely manage personal data and provide better services using blockchain, big data, and AI technology. In this paper, we propose a system that can safely manage personal data by using blockchain technology, which can be used without changing the existing system. The composition of this system consists of a blockchain, blockchain linkages, a service provider, and a user (i.e., an app). Blockchain can be used regardless of its type and form, and services are provided by classifying blockchains and services in the blockchain linkages. Service providers can access personal data only after requesting and receiving delegated permission from users. Existent MyData services store all data in a user's mobile phone, so information may get leaked due to jailbreaks or rooting. But in the proposed system, personal data are stored in blockchain so information leakage can be prevented. In the future, we will study ways to provide customized services using personal data stored in blockchain.

• Journal of Forest and Environmental Science
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• v.37 no.4
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• pp.304-308
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• 2021

Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.213-221
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• 2022

Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.

• Journal of Ginseng Research
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• v.46 no.5
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• pp.621-627
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• 2022

Background: Panax ginseng (ginseng) is a traditional medicine that is reported to have cardioprotective effects; ginsenosides are the major bioactive compounds in the ginseng root. Methods: Magnetic molecularly imprinted polymer (MMIP) nanoparticles might be useful for both the extraction of the targeted (imprinted) molecules, and for the delivery of those molecules to cells. In this work, plant growth regulators were used to enhance the adventitious rooting of ginseng root callus; imprinted polymeric particles were synthesized for the extraction of ginsenoside Rb₁ from root extracts, and then employed for subsequent particle-mediated delivery to cardiomyocytes to mitigate hypoxia/reoxygenation injury. Results: These synthesized composite nanoparticles were first characterized by their specific surface area, adsorption capacity, and magnetization, and then used for the extraction of ginsenoside Rb₁ from a crude extract of ginseng roots. The ginsenoside-loaded MMIPs were then shown to have protective effects on mitochondrial membrane potential and cellular viability for H9c2 cells treated with CoCl₂ to mimic hypoxia injury. The protective effect of the ginsenosides was assessed by staining with JC-1 dye to monitor the mitochondrial membrane potential. Conclusion: MMIPs can play a dual role in both the extraction and cellular delivery of therapeutic ginsenosides.