• Title/Summary/Keyword: Rooting

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Promoted rooting of softwood cuttings of highbush blueberry 'Duke', 'Elizabeth', 'Elliot' (Vaccinium corymbosum L.) as affected by indole-3-butyric acid(IBA) dipping treatment (Indole-3-butyric acid(IBA) 침지처리에 의한 highbush blueberry 'Duke', 'Elizabeth', 'Elliot' (Vaccinium corymbosum L.)의 녹지삽목 발근 촉진)

  • Kang, S.K.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.17 no.1
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    • pp.145-155
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    • 2015
  • To investigate the practicality of a small scale softwood propagation plastic film tent and the rooting promoting effect of indole-3-butyric acid(IBA) for northern highbush blueberry, softwood cuttings of 3 varieties (Duke, Elizabeth, Elliot) were made after dipping in the IBA solutions of 100ppm, 300ppm, 500ppm, 1,000ppm for 2 hour, and 3,000ppm for 20 seconds, respectively. Two-way ANOVA confirmed the significant differences in the percentage of rooting of cutting, longest root length and rooting volume index (RVI) induced by IBA treatment, as well as the percentage of rooting of cutting and RVI among examined varieties. In particular, the highly significant difference was observed in th RVI for IBA treatment. However, there was no interactions between the auxin treatments and varieties. Spontaneous rooting ability (control) depends on the varieties, showing that the lowest rooting ability was observed in the 'Duke' (45%) followed by 'Elliot'(85%) and 'Elizabeth' (90%). In addition, the rooting of the examined 3 varieties were enhanced by both of short time (20sec) dipping treatment of low level solutions, and long time (2hr) dipping treatment of high level solution, with the remarkably promoted rooting of the 'Duke' cuttings.

In vitro Propagation and Ex vitro Rooting of Tectona grandis (L.f ), APNBV-1 Clone

  • Ramesh, Kommalapati;Chandra, Mouli Kalla;Vijaya, Tartte
    • Journal of Forest and Environmental Science
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    • v.25 no.2
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    • pp.119-126
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    • 2009
  • An efficient in vitro plant regeneration system was developed through shoot proliferation from axillary buds of Tectona grandis (L.f), APNBV-1 (Andhra Pradesh North Badrachalam Venkatapuram-1) clone. Multiple shoots of high quality were produced in vitro from axillary bud explants. An average of 4.39 shoots/explant were obtained on Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) benzyl amino purine (BA), kinetin (KN), indole acetic acid (IAA), gibberillic acid ($GA_3$), growth adjuvants casein hydrolysate (CH), adenine sulphate (Ads) and antioxidants ascorbic acid, polyvinyl pyrrollidine (PVP). Eighty five percent of rooting was observed in ex vitro rooting media containing IBA and vermiculite. In ex vitro rooting, single shoots with 2 to 3 nodes were subjected to IBA of different concentrations at different periods of time intervals. Direct rooting in vermiculite at 500 ppm concentration of IBA resulted in 4.3 number of roots with 2 cm length. Minimum response of rooting and length of roots were recorded at 100 ppm concentration of IBA. Planlets were transferred to plastic bags for short acclimatization stage in green house where they survived at 95%.

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Effects of Clones, Ortet Age, Crown Position, and Rooting Substance upon the Rooting of Cuttings of Japanese Larch (Larix leptolepis S. et Z. Gordon) (삽수(揷穗)의 클론, 모수령(母樹齡), 채취부위(採取部位) 및 발근촉진제(發根促進劑)가 낙엽송(落葉松)(Larix leptolepis S. et Z. Gordon)의 삽목발근(揷木發根)에 미치는 영향(影響))

  • Chung, Durk Young;Lee, Kyung Joon
    • Journal of Korean Society of Forest Science
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    • v.83 no.2
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    • pp.205-210
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    • 1994
  • This study was conducted to develop a method of mass production by cutting of superior genotypes of Larix leptolepis and to examine the effects of ortet age, clone, crown position, and an applied plant growth substance upon the rooting of cuttings. Four different ortet ages, 2, 8, 16 and 30 years and three levels of root-promoting substances, indole butyric acid(IBA), at 1000, 2000, and 5000ppm were employed. Greenwood cuttings were taken from mother trees in late July and rooted for three months in rooting medium containing peatmoss : vermiculite : perlite(1 : 1 : 1 by volume). with temperature controlled at $20-25^{\circ}C$, humidity by intermittent misting and light by partial shading in the greenhouse. Cuttings treated with 1000ppm IBA showed highest rooting, with 80%, 71%, 52%, 25% for 2-, 8-, 16-. 30-year-old ortet, respectively. Untreated cuttings showed rooting of 52%, 48%, 36%, 20% for the ortet age of 2, 8, 16, 30 years, respectively. The average number of adventitious roots decreased with increasing ortet age from 2 to 30 years, whereas IBA treatment increased the number of roots in all ages. A variation in rooting among 20 clones tested was observed. The 4 clones, Chungnam 6, and 7 and Chonbuk 1, and 9 showed good rooting of about 67%, while Kangwon 49 showed boor rooting of 40% at 1000ppm IBA treatment. When crown position was compared, cuttings taken from middle crown showed highest rooting of 60%, while cuttings from upper crown showed lowest rooting of 53%.

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Effect of Cutting Position, Media and Auxin on Rooting of Leaf Variegated Artemisia keiskeana Stem Cuttings (잎무늬종 맑은대쑥의 줄기삽목시 삽수부위, 용토 및 오옥신 처리가 발근에 미치는 영향)

  • Youn, Hee-Jung;Lee, Jong-Suk
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.1
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    • pp.29-32
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    • 2010
  • This study was conducted to determine the effects of cutting position, media, and auxin on rooting of Artemisia keiskeana cuttings. In the cutting positions, the cutting of middle and basal positions had a better growth in the both upper and lower part than the cutting of the top position. Especially, the cutting of the middle position showed the highest rooting rate, at 68.3%. In the media, peatmoss showed the latest date on the rooting initiation. Also, the growth, survival rate and rooting rate of the upper part and lower part were low. However, the cutting in vermiculate showed the fastest date on the rooting initiation, and had a significant effect on the growth of the upper part and lower part, showing the highest rooting rate and survival rate in all tested media. When cuttings were treated by auxin, the rooting rate and growth of cuttings were higher than the control. The control showed the lowest rooting rate, at 41%, while, the auxin treatment showed the highest, rate over 80%. Whereas when treated by NAA, the lower part of cuttings had a better growth than these treated by IBA. Also, the higher the concentration of auxin was, the higher the rooting rate and growth rate of Artemisia keiskeana cuttings were.

A Study on Propagation and Growth Characteristics of Tamarix chinensis for Development of Plant Using in Coast Environmental Forests (해안 환경림 조성용 식물개발을 위한 위성류의 증식과 생장특성에 관한 연구)

  • Park Chong-Min;Kim Yong-Kil
    • Journal of the Korean Institute of Landscape Architecture
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    • v.34 no.3 s.116
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    • pp.79-90
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    • 2006
  • Tamarix chinenis blooms twice a you and its flowers, branches and leaves make the adjustment of tree shape. Propagation methods and growth characteristics of T. chinensis were studied in order to ascertain its potential use as one of vegetation resources for coast forestation and landscaping. The study results indicated that 1 or 2 you old hard wood cuttings showed higher rooting ratio than greenwood or semi hard wood cuttings. One to one mixture between vermiculite and pearlite appeared to be the best for bed soil, and sea sand and silt(loess) mixture was the next. Sea sand and granitic soil followed after. In terms of seasonal differences, spring cuttings showed the best rooting ratio, root number, and root length. Fall cuttings followed after spring cutting, and summer cuttings showed worst results regarding rooting ratio, root number, and root length. The best rooting promotion effects of growth regulators were observed with sea sand bed soils. There was no significant difference among growth regulators in terms of rooting and shoot growth. Low concentration below 100 ppm of growth regulators was enough for rooting promotion effect. In general, the number and mean length of roots and shoots were showed the excellent records in the sites with high rooting ratio. The study result strongly showed that T. chinensis can be considered as a suitable tree for coast forestation and landscaping because of its easy cutting propagation and rapid growth on saline lands.

Micorpropagation of Corylopsis coreana by Thidiazuron Treatment (Thidiazuron 처리에 의한 히어리나무의 기내번식)

  • Kang, Hyo-Jin;Moon, Heung-Kyu;Yi, Jae-Seon
    • Journal of Plant Biotechnology
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    • v.30 no.3
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    • pp.263-267
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    • 2003
  • This study was conducted to evaluate the effect of thidazuron(TDZ) on shoot proliferation and growth from axillary buds of 20-years-old Corylopsis coreana. Shoots proliferation was effectively achieved on WPM(Woody Plant Medium) supplemented with 0.03∼0.1mg/L TDZ. The highest shoot number(6.5$\pm$0.7) was obtained on 0.1mg/L TDZ treatment. On the TDZ medium shoots formed as clusters less than 1cm in height and therefore needed to subculture on GA$_{3}$ containing medium to induce elongation. In consecutive cultures, phenolic compounds were excreted at the proximal part of the explants and inhibited growth of the explants. Growth inhibition by the compounds was overcome using liquid and paper bridge culture system. About 60% of the elongated shoots rooted on half- strength MS medium containing IBA. Generally, IBA was mire effective on in vitro rooting than NAA with optimal range of 0.5mg/L to 1.0mg/L. Rooted plantlets were transferred in an artificial soil(vermculite) and acclimatized in high humidity greenhouse condition. Survival rate differed greatly depending on rooting types of the explants. Two types of rooting were observed. The first type was direct rooting from the explants. The second type was callus formation followed by rooting from the callus. The explants showing the 1st type rooting survived can be multiplicated in vitro by TDZ treatment followed by elongation with GA$_{3}$ and rooting with IBA.

Change of Rooting Potential as Affected by Rooting Promoter Treatment in Cuttings of Corylopsis coreana (히어리(Corylopsis coreana)의 삽목번식시 발근촉진제의 처리에 따른 발근력 변화)

  • Kwon, Soon-Tae;Kim, Jae-Chang;Jeong, Jeong-Hag
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.4
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    • pp.202-205
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    • 2011
  • This study was conducted to determine the effects of several rooting promoter treatments on rooting of Corylopsis coreana cutttings for mass production. In first year experiment, no effect of rooting promoters was observed in hardwood cuttings taken on March 20. However, In softwood cuttings of June 20, rooting potential was greatly increased by IBA $100mg{\cdot}L^{-1}$ dipping treatment for 24 hours. Particularly, IBA $100mg{\cdot}L^{-1}$ 24 hour dipping treatment greatly shortened the days required for the rooting. In second year experiment, the result of first year was reconfirmed. Almost of all the cuttings (97.8% of rooting rate) were well rooted after only 30 days after cutting by IBA $200mg{\cdot}L^{-1}$ 24 hour dipping treatment. In this treatment, numbers and length of root were also prominent compared with any other treatment results.

Studies on the Conditions of Softwood Cutting for Production of Mulgerry Sapling (뽕나무 묘목생산을 위한 신소삽목 조건 구명)

  • 구태원;성규병
    • Journal of Sericultural and Entomological Science
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    • v.39 no.2
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    • pp.101-105
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    • 1997
  • Most of the mulberry saplings in Korea have been produced by grafting. But the production of mulberry sapling by this method is very complex process and requires high level of technique and two years to produce saplings. Therefore, to develop the mulberry sapling production system by softwood cutting method which is suitable for mass production, several conditions on rooting and growth of cuttings were investigated. The rooting ability of cuttings varied according to mulberry varieties, showing the highest rooting ratio of 93.3% in cultivar of Shingwangppong. Of different soil texture as for rooting media, the clay was found to be the best; other media decreased in order as follows; sand, sand loam and vermiculite. The shading ratio by 70% with polyethylene film showed the highest rooting ratio of the cuttings. Optimum day of cutting was around 50th day after sprouting, whereas the ratio of stem thicker than 7mm at the base of saplings in diameter was highest at 44th day.

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Promotion of Rootability of the Cuttings of Dendropanax morbifera Lev (황칠나무의 揷木發根力 增進)

  • 김세현
    • Korean Journal of Plant Resources
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    • v.11 no.2
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    • pp.157-162
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    • 1998
  • This study was conducted to examine the effects of media, crown position, clone ,a nd plant growth substances upon the rootability of cuttings for the establishment of the method of mass production by cutting of Dendropanax morbifera Lev. the artifical soil mixtureof vermiculite, perlite and peatmoss (1 : 1 : 1 v/v/v) with IBA 100mg /l treatment was most effective on the rooting rate (85%) was obtained with semihardwood cuttings, which were collected early-August after the first growing seasons. The effect of crown position on rooting of cuttings were obsreved. Average percentage rooting of cuttings on crown position showed 78% , 72% and 65% in middle , upper and lower part with IBA 100mg/l treatment , respectively. Among the four treatment levels of IBA, the highest rooting rate was observed in 100mg/l IBA treatment.

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The Study of the Root Cutting Propagation of Kalopanax pictus Nakai (음나무 삽목 번석에 관한 연구)

  • Jung Sang Duk;Sung Jung Sook;Huh Man Kyu
    • Journal of Life Science
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    • v.15 no.1 s.68
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    • pp.21-26
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    • 2005
  • This study officially selected Kalopanax pictus. The root cutting and investigation conducted how the production process of the rooting ratio according to its age and its length gives some influence to the rooting ratio and what is the usage value in the effect of rooting ratio of an accelerant of plant growth and IBA treatment. The ages and ratio of rooting are one year $92.0\%$, two years $80.0\%$, and three years $67.0\%$. As shown above, one year cutting showed the highest growth. The rooting ratio by the length of the cutting-tree is $75.0\%$ in 5cm, $92.0\%$ in 10cm and $89.0\%$ in 15cm. There is a little differences according to the result of each analysis in the rooting ratio by the length of the cutting tree. Two groups having the length 10cm and 15cm showed the similar rooting ratio, while the group having the length 5cm showed that the rooting ratio is very low. The rooting ratio treated by the hormone, IBA showed that the treated group was $94\%$ and the untreated group was $92\%$. Although the treated group showed a little high rooting growth ratio, there was no significant difference. Thus, it is said that the treatment of IBA in the cutting tree is insignificant.