• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.6
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• pp.757-767
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• 1995

This experiment was done to determine the optimum concentration of IAA for root development in plants regenerated from the callus culture of barley embryos. Two concentrations of 2,4-D, 3ppm and 5ppm selected as an optimum among five different concentrations in the previous experiment were used for callus induction and proliferation in this experiment. For callus induction, 3ppm of 2,4-D produced 35.6% in immature embryos and 4.4% in mature embryos, while 5ppm gave 33.8% in immature and 5.6% in mature embryos. Out of 320 immature embryos cultured, 111 embryos were induced to calli and 684 plants were produced from them, while only 16 embryos were induced to calli from 320 mature embryos and 92 plants were restored. The rates of callusing and plant regeneration were 34.7%, 214% in immature embryos and 5.0%, 28.7% in mature embryos, respectively. The average root lengths and root numbers of plants restored from callus at five different IAA concentrations of 0ppm, Ippm, 5ppm, l0ppm and 30ppm were 7.9mm, 3.6; 18.4mm, 5.2; 16.1mm, 3.9; 8.5mm, 3.5 and 6.4mm, 3.4, while plants directly obtained from mature embryos were 14.8mm, 4.9; 4.9mm, 3.6; 4.3mm, 3.1; 3.6mm, 2.6 and 3.2mm, 2.1, respectively. Therefore, 1ppm gave the best result for the root. promotion in callus, whle 0ppm, a control, gave the largest root developmemt in embryos. High concentration of lAA(30ppm) in callus and any exogeneous supplement of lAA in embryos negatively affected to the root lengths and root numbers. Genotypic effect was also observed in given four varieties, Bruce, Klages, Olbori and Albori. For chromosome doubling, when 0.1% colchicine was applied on 428 plants under three different conditions such as air circulation, temperatures and growth stages, 319 plants of doubled haploids were obtained so that the rate was 74.5%

• Journal of the korean academy of Pediatric Dentistry
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• v.8 no.1
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• pp.65-75
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• 1981

The author have studied on the pulp responses to the electric pulp stimulator (Pyo-Cure)(R) in the primary molar age from 4 to 11 of the 186 children (male 100, female 86). The total numbers of teeth used in this study were 1096. The results were as follows ; 1. The difference in responses between the teeth of the male and female was not statistically significant. 2. The difference in responses between the teeth of the right and left sides was not statistically significant. 3. The difference in responses between the teeth of the upper and lower jaws was not statistically significant. 4. The responses of the teeth in the older age is higher than its of the early age. 5. Inspite of advanced root resorption of the primary molars, except severe root resorption, the responses of the teeth was higher according to increasing the age. 6. The responses of the teeth were decreased severely in the primary molars as it becomes exfoliation time.

• Communications for Statistical Applications and Methods
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• v.16 no.6
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• pp.1037-1046
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• 2009

Copy number variations(CNVs) are known as one of the most important factors in susceptibility to genetic disorders because they affect expression levels of genes. In previous studies, pyrosequencing, mini-sequencing real-time polymerase chain reaction(PCR), invader assays and other techniques have been used to detect CNVs. However, the higher the copy number in a genome, the more difficult it is to resolve the copies, so a more accurate method for measuring CNVs and assigning genotype is needed. PCR followed by a quantitative oligonucleotide ligation assay(qOLA) was developed for quantifying CNVs. The aim of this study was to compare the two methods for detecting and quantifying the CNVs of duplicated gene: the published pyrosequencing assay(pyro_CNV) and the newly developed qOLA_CNV. The accuracy and precision of the assay were evaluated for porcine KIT, which was selected as a model locus. Overall, the root mean squares(RMSs) of bias and standard deviation of qOLA_CNV were 2.09 and 0.45, respectively. These values are less than half of those of pyro CNV.

• International Journal of Naval Architecture and Ocean Engineering
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• v.11 no.2
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• pp.952-969
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• 2019

This study develops new analytical solutions to water wave diffraction by vertical truncated cylinders in the context of linear potential theory. Three typical truncated surface-piercing cylinders, a submerged bottom-standing cylinder and a submerged floating cylinder are examined. The analytical solutions utilize the multi-term Galerkin method, which is able to model the cube-root singularity of fluid velocity near the edges of the truncated cylinders by expanding the fluid velocity into a set of basis function involving the Gegenbauer polynomials. The convergence of the present analytical solution is rapid, and a few truncated numbers in the series of the basis function can yield results of six-figure accuracy for wave forces and moments. The present solutions are in good agreement with those by a higher-order BEM (boundary element method) model. Comparisons between present results and experimental results in literature and results by Froude-Krylov theory are conducted. The variation of wave forces and moments with different parameters are presented. This study not only gives a new analytical approach to wave diffraction by truncated cylinders but also provides a reliable benchmark for numerical investigations of wave diffraction by structures.

• Proceedings of the KSRS Conference
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• v.1
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• pp.278-281
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• 2006

Korea Meteorological Administration(KMA) has issued the tropical storm(typhoon) warning or advisories when it was developed to tropical storm from tropical depression and a typhoon is expected to influence the Korean peninsula and adjacent seas. Typhoon information includes current typhoon position and intensity. KMA has used the Dvorak Technique to analyze the center of typhoon and it's intensity by using available geostationary satellites' images such as GMS, GOES-9 and MTSAT-1R since 2001. The Dvorak technique is so subjective that the analysis results could be variable according to analysts. To reduce the subjective errors, QuikSCAT seawind data have been used with various analysis data including sea surface temperature from geostationary meteorological satellites, polar orbit satellites, and other observation data. On the other hand, there is an advantage of using the Subjective Dvorak Technique(SDT). SDT can get information about intensity and center of typhoon by using only infrared images of geostationary meteorology satellites. However, there has been a limitation to use the SDT on operational purpose because of lack of observation and information from polar orbit satellites such as SSM/I. Therefore, KMA has established Advanced Objective Dvorak Technique(AODT) system developed by UW/CIMSS(University of Wisconsin-Madison/Cooperative Institude for Meteorological Satellite Studies) to improve current typhoon analysis technique, and the performance has been tested since 2005. We have developed statistical relationships to correct AODT CI numbers according to the SDT CI numbers that have been presumed as truths of typhoons occurred in northwestern pacific ocean by using linear, nonlinear regressions, and neural network principal component analysis. In conclusion, the neural network nonlinear principal component analysis has fitted best to the SDT, and shown Root Mean Square Error(RMSE) 0.42 and coefficient of determination($R^2$) 0.91 by using MTSAT-1R satellite images of 2005. KMA has operated typhoon intensity analysis using SDT and AODT since 2006 and keep trying to correct CI numbers.

• Journal of Embryo Transfer
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• v.30 no.1
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• pp.23-31
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• 2015

Catalpol, an iridoid glucoside, isolated from the root of Rehmannia glutinosa Libosch. It possesses a broad range of biological and pharmacological activity including anti-tumor, anti-inflammation and anti-oxidant by acting as a free radical scavenger. Therefore, in this study, the effects of catalpol on blastocyst development, expression levels of reactive oxygen species (ROS) and apoptotic index were investigated in porcine embryos. After in vitro maturation and fertilization, porcine embryos were cultured for 6 days in porcine zygote medium 3 (PZM-3) supplemented with catalpol (0, 100, 200 and $400{\mu}M$, respectively). Blastocyst development not significantly improved in the catalpol treated group when compared with control group. Otherwise, the intracelluar levels of ROS were decreased and the numbers of apoptotic nuclei were reduced in the catalpol ($100{\mu}M$) treated porcine blastocysts (P<0.05). On the other hand, blastocyst development was significantly improved in the catalpol ($100{\mu}M$) treated group when compared with the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Otherwise, the intracellular levels of ROS in catalpol ($100{\mu}M$) treated group were significantly decreased in the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Furthermore, the total cell numbers of blastocysts were significantly increased (P<0.05) in the catalpol ($100{\mu}M$) treated group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress, whereas numbers of apoptoic nuclei were significantly reduced (P<0.05). In conclusion, our results indicate that treatment of catalpol may have important implications for improving developmental competence and preimplantation quality of porcine embryos through its anti-oxidant and anti-apoptotic effect.

• Journal of Embryo Transfer
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• v.28 no.3
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• pp.243-250
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• 2013

Pyracantha is a genus of thorny evergreen large shrubs in the family of Rosaceae, with common names Firethorn or Pyracantha. It's extract has also been used in cosmetics as a skin-whitening agent and functioning through tyrosinase inhibition. Recent studies have shown that pyracantha extract possesses antioxidant activities and may significantly improve lipoprotein metabolism in rats. Although the mode of action of Pyracantha extract is not fully understood, a strong relationship was observed between antioxidant and apoptosis in some types of cells. Thus, the aim of this study was to evaluated the effect of pyracantha extract on blastocysts formation and their quality of the porcine parthenogenetic embryos. After parthenogenetic activation by chemicals, presumptive porcine parthenogenetic embryos were cultured in PZM-3 medium supplemented with extracts of pyracantha leaf, stalk and root for 6 day (1, 5 and $10{\mu}g/ml$, respectively). In our results, the frequency of blastocyst formation in pyracantha root extract ($5{\mu}g/ml$) treated group had increased that of other groups. Furthermore, blastocysts derived from pyracantha root extract ($5{\mu}g/ml$) treated group had increased the total cell numbers and reduced apoptotic index. Blastocyst development was significantly improved in the pyracantha root extract ($5{\mu}g/ml$) treated group when compared with the $H_2O_2$ treated group (p<0.05). Subsequent evaluation of the intracellular levels of ROS in pyracantha root extract ($5{\mu}g/ml$) treated groups under $H_2O_2$ induced oxidative stress were decreased (p<0.05). In conclusion, our results indicate that treatment of pyracantha root extract may improve in vitro development of porcine parthenogenetic embryos through its antioxidative and antiapoptotic effects.

• Journal of Korean society of Dental Hygiene
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• v.16 no.4
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• pp.635-641
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• 2016

Objectives: The purpose of this study is to assess the characteristics of tooth surface after using Gracy curet and Ultrasonic scaler Methods: In this study, 12 teeth extracted were used. 12 specimens were divided into three groups with the same numbers, which were classified into Control group, Gracy curet use group, and Ultrasonic scaler use group, and after performing instrument operation, we measured the roughness and the loss degree of tooth surface by using SEM. Results: In groups using Gracy curet and Ultrasonic scaler, the roughness and the loss of tooth surface increased significantly(p<0.05). In the roughness of groups using Gracy curet and Ultrasonic scaler, Ultrasonic scaler group was higher in crown, but Gracy curet group was higher in root. As a result of observation through SEM, the roughness and the loss degree increased in order of Control group, Ultrasonic scaler use group, and Gracy curet use group. Conclusions: Taken together above results, both hand instrument and ultrasound equipment create roughness and loss in crown and in root, and hand instruments makes rougher than ultrasonic instruments in root, so it is thought to require thorough and accurate technical application not to damage tooth surface when removing plaque.

• Korean Journal of Weed Science
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• v.32 no.3
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• pp.230-239
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• 2012

Coconut (Cocos nucifera L) is one of the predominant plantation crops in Sri Lankan economy which is known to have existed for over thousands of years. During the past decades coconut production had been reduced by a significant quantity. The usage of poor quality planting materials is a major reason for the low coconut production. Thus much attention needs to be paid in coconut nurseries. Weed management is a critical management practice in the nursery. Though glyphosate application is becoming popular in nurseries it can affect weeds as well as coconut seedlings growth. Therefore the effects of glyphosate were evaluated by determining the growth of shoot and root of coconut seedlings. Poly bag nursery was prepared and three treatments were used. Treatments were no glyphosate and manual weeding ($T_1$), application of glyphosate 1.08 ai kg $ha^{-1}$ at 2 monthly interval ($T_2$) and application of glyphosate 1.44 ai kg $ha^{-1}$ at 2 monthly interval ($T_3$). Application of glyphosate at early stage of seedling growth had a no significant effect on growth parameters tested. However, the concentrations of glyphosate negatively affected numbers, volumes and dry weights of secondary, tertiary and quaternary roots at the latter stage of seedling growth. The leaf area and the height of seedling were significantly reduced by the highest concentration of glyphosate. Among the growth parameters tested, seedling girth and shoot dry weight were not affected by the application of glyphosate. These results revealed that the usage of glyphosate at both concentrations negatively affected root growth of coconut seedlings. Based on these results, the both concentration levels of glyphosate should be applied to coconut nurseries before sprouting the seed nuts.

• Restorative Dentistry and Endodontics
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• v.20 no.1
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• pp.71-95
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• 1995

Bacteria have been regarded as a one of major etiologic factors in root canal infections. In endodontic treatment the effective removal of pathogenic microorganisms in the root canal is the key to successful outcome. Bacterial cell wall components may play an important role in the development of pulpal and periapical disease. The purpose of this study was to evaluate the effect of sonic extracts of Streptococcus spp. on cultured L929 cells and to characterize cell wall protein profiles of Streptococcus spp. Streptococcus spp. were isolated from infected root canals and identified with Vitek Systems(Biomeriux, USA). Five streptococci, namely S. sanguis, S. mitis, S uberis, S. mutans (ATCC 10449) and S. faecalis (ATCC 19433) weere enriched in brain heart infusion broth. Cell pellets were sonicated and cell wall extracts were dialyzed and membrane filtered. Prepared cell wall proteins were applied to cultured L929 cell. The cell reaction were evaluated by monitoring DNA synthesis, cell numbers and the change of cell morphology. The total cell wall protein profiles of microorganisms were characterized by sodium dodecyl sulfate polyacrylamide-gel eledruphoresis(SDS-PAGE). DNA synthesis of L929 cells were reduced by the increasing concentration of sonic extracts. DNA synthesis was significantly suppressed in more than $50{\mu}g$/ml of sonic extract conentration in five streptococci. S. nutans (ATCC 10449) showed stronger suppression on DNA synthesis than remaining four streptococci, which had the similar effect on DNA synthesis. Analysis of DNA synthesis measured by [$^3H$]-thymidine uptake was more sensitvie method than cell counting. Sonic extracts affected the microscopic findings of L929 cells. The protein profiles indicated that all five strains shared two major proteins with molecular masses of 70.8 and 57.5 kD respectively. S. uberis and S. mutans shared common minor proteins of which molecular weights were 147.9 and 112.2 kD respectively. However some minor proteins were unique for S. mitis, S. uberis and S. faecalis.