• Korean Journal of Environmental Agriculture
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• v.38 no.3
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• pp.185-196
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• 2019

BACKGROUND: This study investigated the effects of rice genetically modified to be resistant against rice blast and rice bacterial blight on the soil microbial community. A comparative analysis of the effects of rice genetically modified rice choline kinase (OsCK1) gene for disease resistance (GM rice) and the Nakdong parental cultivar (non-GM rice) on the soil microbial community at each stage was conducted using rhizosphere soil of the OsCK1 and Nakdong rice. METHODS AND RESULTS: The soil chemistry at each growth stage and the bacterial and fungal population densities were analyzed. Soil DNA was extracted from the samples, and the microbial community structures of the two soils were analyzed by pyrosequencing. No significant differences were observed in the soil chemistry and microbial population density between the two soils. The taxonomic analysis showed that Chloroflexi, Proteobacteria, Firmicutes, Actinobacteria, and Acidobacteria were present in all soils as the major phyla. Although the source tracking analysis per phylogenetic rank revealed that there were differences in the bacteria between the GM and non-GM soil as well as among the cultivation stages, the GM and non-GM soil were grouped according to the growth stages in the UPGMA dendrogram analysis. CONCLUSION: The difference in bacterial distributions between Nakdong and OsCK1 rice soils at each phylogenetic level detected in microbial community analysis by pyrosequencing may be due to the genetic modification done on GM rice or due to heterogeneity of the soil environment. In order to clarify this, it is necessary to analyze changes in root exudates along with the expression of transgene. A more detailed study involving additional multilateral soil analyses is required.

• Korean Journal of Weed Science
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• v.30 no.4
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• pp.412-420
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• 2010

Weeds are known to cause enormous losses due to their interference in agro ecosystems. Because of environmental and human health concerns, worldwide efforts are being made to reduce the heavy reliance on synthetic herbicides that are used to control weeds. In this regard phytotoxicity of allelochemical sorgoleone, which is a major component of the hydrophobic root exudates of Sorghum bicolor was evaluated in different weed species and also its crop selectivity in greenhouse and field conditions. Sorgoleone strongly inhibited the growth of different weeds by pre-emergence and post-emergence applications both in greenhouse and field conditions. Post-emergence application of sorgoleone on 21-day-old weed seedlings had a greater inhibitory effect than the pre-emergence application. Again, broadleaf weed species were more susceptible than grass species to the application of sorgoleone at both stages of growth. Growth of broadleaf weed species was suppressed by greater than 80% for most of the weed species except a few species and among them the species Rumex japonicus and Galium spurium were completely suppressed at $200{\mu}g\;ml^{-1}$ sorgoleone. Like greenhouse trial, sorgoleone was more effective for broadleaf weed species followed by sedge and grass weed species in the field condition. The growth inhibition of weeds was slightly lower in field condition compared to greenhouse condition. The crop species like rice, barley, wheat, corn, perilla, tomato, soybean and Chinese cabbage were tolerant to sorgoleone while lettuce and cucumber were slightly susceptible to sorgoleone. Consequently, sorgoleone may be applied to control weeds in organic farms without affecting the growth of crop.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.3
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• pp.257-264
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• 1997

To find out the influence of different rice varieties on the emission of methane in the soil and the exudation of carbohydrates from root, the experiment was conducted on a fine silty, mesic family of Aeric Fluventinc Haplaquepts (Jeonbug series). The varities of rise involved one early maturing variety of Japonica type(namweon) and three mid-to-late maturing varieties of Japonica type and one mid-to-late variety of Indica${\times}$Japonica crossed. The methane flux over the rice canopy was measured according to the closed chamber method modified by Shin and the methane concentration in the soil was measured using porous cup installed in the soil. The carbohydrate exudates from root were measured under nutrient solution culture. It was found that the methane flux tended to be lower in early maturing varieties than in the mid-to-late maturing varieties, lower in indica${\times}$Japonica crossed variety than in Japonica type varieties. There was positive correlation between the number of tillers and the weight of roots of rice plant and methane flux. The correlation, however, tended to be greater between the weight of roots and methane flux. There was no significant difference in the concentration of methane in the soil under different varieties of rice, excepting one variety, Kehwa under which methane concentration was highest. In case of carbohydrate exudation, early maturing variety tended to be higher than other varieties, although the opposite was the case in methane flux.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.4
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• pp.329-336
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• 1989

A series of green house experiment was conducted to find but the effect of fertilizer application and inoculation of rhizobium on the changes of amide-N, ureide-N and $NH_4-N$ concentration in stem and root exudates of soybean plant growth. The results obtained were summarized as follows ; 1. Five strains of indigenous Rhizobium japonicum-nitrogen fixing activity($C_2H_2$-reducing activity) was more than 6.4 to 20.1 nmole/hr/tube-were identified from 37 soil samples in 22 areas of farmers field throughout country. 2. These identified 5 strains of rhizobium were obtained high nitrate reductase but low ammonium and nitrite oxidase activities. Among 5 strains of rhizobium the Rhizobium japonicum RjK-134 was applied for this green house experiment. 3. Dry matter yield was increased by the combination of inoculation of Rhizobium japonicum RjK-134 with no fertilizer and without nitrogen fertilizer application. However, dry matter yield was decreased with application of N and NPK with inoculation of rhizobium. 4. The concentrations of amide-N and ureide-N were increased in xylem sap than that of root exudate and higher concentration was obtained ar 30 days after planting than flowering stage (45 days after planting). 5. The combination of NPK application with inoculation of Rhrizobium japonicum RjK-134 enhanced the increase of amide-N and ureide-N concentration in xylem sap and root exudate. 6. High ammonium-N concentration in xylem sap and root exudate were obtained in combination with without-fertilizer under no inoculation of rhizobium and N and NPK application with inoculation of rhizobium.

• Microbiology and Biotechnology Letters
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• v.34 no.2
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• pp.83-93
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• 2006

In heavily industrialized areas, soil sites are contaminated with high concentrations of heavy metals. These pollutants are highly accumulated to the human body through the food web and cause serious diseases. To remove heavy metals from the soil, a potential strategy is the environmental friendly and cost effective phytoremediation. For the enhancement of remediation efficiency, the symbiotic interaction between the plant and plant growth-promoting rhizobacteria (PGPR) has been attended. In this review, the interaction of the plant and PGPR in the heavy metal-contaminated soil has been reviewed. The physicochemical and biological characteristics of the rhlzosphere can influence directly or indirectly on the biomass, activity and population structure of the rhizobacteria. The root exudates are offered to the soil microbes as useful carbon sources and growth factors, so the growth and metabolism of rhizobacteria can be promoted. PGPR have many roles to lower the level of growth-inhibiting stress ethylene within the plant, and also to provide iron and phosphorus from the soil to plant, and to produce phytohormone such as indole acetic acid. The plant with PGPR can grow better in the heavy metal contaminated soil. Therefore higher efficiency of the phytoremediation will be expected by the application of the PGPR.

• The Korean Journal of Pesticide Science
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• v.3 no.2
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• pp.29-36
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• 1999

The leaching behaviour of $^{14}C$-paraquat in soil was investigated using soil columns (5 cm I.D. ${\times}$ 30 cm H.) parked with two soils of different physicochemical properties. $^{14}C$-Activities leached from the soil A (loam) columns with and without rice plants for 117 days were 0.42 and 0.54% of the originally applied, whereas those from the soil B (sandy loam) were 0.21 and 0.31%, respectively. $^{14}C$-Activities absorbed by rice plants from soil A and B were 3.87 and 2.79%, respectively, most of which remained in the root. Irrespective of soil types, more than 96% of the total $^{14}C$ resided in soil, mostly in the depth of $0{\sim}5$ cm. The water-extractable $^{14}C$ in soil was in the range of $6.10{\sim}9.01%$ of the total $^{14}C$ applied. The rest of $^{14}C$, which corresponds to non-extractable soil residues of [$^{14}C$]paraquat, was distributed in humic substances in the decreasing order of humin>humic acid>fulvic acid. The soil pH of the columns without rice plants increased after the leaching experiment due to the flooded anaerobic condition resulting in the reduction of the $H^{+}$ concentration, whereas that of the columns with rice plants did not increase by the offsetting effect of the acidic exudates from the roots. Low mobility of paraquat in soil strongly indicates that no contamination of ground water would be caused by paraquat residues in paddy soils under normal precipitation.

• Journal of Ginseng Research
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• v.40 no.1
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• pp.28-37
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• 2016

Background: Panax ginseng cannot be cultivated on the same land consecutively for an extended period, and the underlying mechanism regarding microorganisms is still being explored. Methods: Polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) and BIO-LOG methods were used to evaluate the microbial genetic and functional diversity associated with the P. ginseng rhizosphere soil in various cultivation ages and modes. Results: The analysis of microbial diversity using PCR-DGGE showed that microbial communities were significantly variable in composition, of which six bacterial phyla and seven fungal classes were detected in P. ginseng soil. Among them, Proteobacteria and Hypocreales dominated. Fusarium oxysporum, a soilborne pathogen, was found in all P. ginseng soil samples except R0. The results from functional diversity suggested that the microbial metabolic diversity of fallow soil abandoned in 2003was the maximum and transplanted soil was higher than direct-seeding soil and the forest soil uncultivated P. ginseng, whereas the increase in cultivation ages in the same mode led to decreases in microbial diversity in P. ginseng soil. Carbohydrates, amino acids, and polymers were the main carbon sources utilized. Furthermore, the microbial diversity index and multivariate comparisons indicated that the augmentation of P. ginseng cultivation ages resulted in decreased bacterial diversity and increased fungal diversity, whereas microbial diversity was improved strikingly in transplanted soil and fallow soil abandoned for at least one decade. Conclusion: The key factors for discontinuous P. ginseng cultivation were the lack of balance in rhizosphere microbial communities and the outbreak of soilborne diseases caused by the accumulation of its root exudates.

• Journal of Bio-Environment Control
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• v.14 no.2
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• pp.89-94
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• 2005

This study was conducted to investigate the effect of nonwoven fabrics weight(Ounce) on growth, quality and yield of oriental melon (Cucumis melo L. var. makwa Mak.). Seedling of 'Gumssaragi-Eunchun' was grafted on 'Shinthozoa' root stock. In this study, The minimum air temperature in 6 ounce at night was $4.8^{\circ}C,\;6.9^{\circ}C$ in 9 ounce, $7.9^{\circ}C$ in 12 ounce and $8.0^{\circ}C$ in 15 ounce, respectively. Leaf length, stem diameter, leaf numbers, leaf area, fresh weight and dry weight for 30 days after planting were better in high minimum air temperature at night than in low minimum air temperature, particularly leaf areas of the plant in 9 ounce was $370cm^2$ at 30 days after planting, $116\%$ in 12 ounce, $129\%$ in 15 ounce. The xylem exudates amount in 9 ounce for 24 hours just after basal stem abscission was 10.1mg. It was 1.2 times much in 12 ounce and 1.9times much in 15 ounce than in 9 ounce at 30 days after planting. The blooming of female was faster by 6 days in 15 and 12 ounce but was delayed by 3 days in 6 ounce than 9 ounce, and the days of blooming to harvesting were shorter by 3 days in 15 ounce and 4 days in 12 ounce but was delayed by 3 days in 6 ounce than in 9 ounce. Fruit weight, fruit length, fruit diameter, flesh thickness, soluble solids, and total yield was the highest in 15 ounce followed by 12 ounce,9 ounce and 6 ounce. Fermented fruit rate was the highest in 6 ounce followed by 9 ounce, 12 ounce and 15 ounce, and marketable fruit rates were 15, 12, 9 and 6 ounce in order. Compared to 1,781kg yield per 10a of 9 ounce, $19\%\;and\;49\%$ was increased under 12 ounce and 15 ounce but $47\%$ were decreased 6 ounce.