• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2010년도 추계학술대회
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• pp.657-659
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• 2010

관중의 근경, 감초의 뿌리, 고삼의 뿌리, 진피의 과피로 부터 에탄올로 추출물을 분리하였다. 이들 4종류의 에탄올 추출물을 이용하여 Streptococcus mutans에 대한 항균활성을 조사하였다. 그 결과 관중의 근경, 감초의 뿌리, 고삼의 뿌리는 Streptococcus mutans에 대하여 항균활성을 나타냈다. 그 중 관중과 고삼의 추출물 300ug/disk 농도는 Streptococcus mutans에 대한 뚜렷한 항균활성을 나타냈다.l 이 결과 관중의 추출물은 Streptococcus mutans에 대한 황균 활성물질로 잠재성이 있음을 알 수 있었다.

• 한국자원식물학회지
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• 제17권3호
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• pp.278-282
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• 2004

한방에서 당귀로 쓰여지고 있는 참당귀(Angelica gigas)와 일당귀 (A. acutiloba)의 추출물로 3종의 그람양성균, 2종의 그람음성균과 1종 효모에 대한 항균활성을 조사한 결과는 다음과 같다. 참당귀 지상부와 지하부 추출액의 항균활성은 ethyl acetate 분획물에서만 나타났는데 지상부 추출액에서는 그람양성균인 S. aureus에 대한 항균활성이 가장 강했고, 지하부 추출물에서는 그람양성균인 B. subtilis와 그람음성균인 E. coli에 대한 항균활성이 가장 강했다. 일당귀 추출액의 항균활성은 지상부 추출액은 n-hexane 분획물에서 지하부 추출액 은 water분획물에서 전혀 나타나지 않았으며 지상부와 지하부 추출액의 ether분획물에서 가장 강했다. 효모 S. cerevisiae에 대해서는 항균환성이 전혀 나타나지 않았다.

• Journal of Ecology and Environment
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• 제33권4호
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• pp.299-306
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• 2010

The use of local seed provenance is often recommended in forest restoration. Early vigor is a combination of the performance of seed germination and emergence after planting. The ability of young Brant's oak plants to grow and develop after emergence and its dependence on local habitat conditions was investigated in this study. The effects of seed source and shade on early growing seedlings of Brant's oak (Quercus brantii L.) were determined in field measurements. Seeds of Quercus brantii L. were collected from 4 forest areas (seed sources) in southern Zagros (Provinces of Kohkilouyeh-Bouyer Ahmad and Fars) at altitudes of 850, 1,100, 1,500, 2,100 m a.s.l., and planted in a nursery constructed in southwestern Iran. According to a split-plot design consisting of four blocks, each containing two main treatment plots (no shading, partial shading), each main plot was sub-divided into four sub-plots (for elevations of 850, 1,100, 1,500 and 2,100 m). Results showed that shade treatments had significant effects on emergence percentage and rate, shoot length, shoot dry weight (SDW), root dry weight (RDW), leaf area (LA), and chlorophyll content. Ecological factors also had an effect on seed performance. Altitude of seed source had a very significant effect on root length, LA, SDW, and RDW. The seeds collected from 850 m a.s.l. elevation showed the highest performance, especially in leaf area, root length, shoot dry weight, and root dry weight. Our results showed that the altitude of 850 m a.s.l. was the best for collecting Brant's oak seeds.

• 생약학회지
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• 제48권1호
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• pp.31-37
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• 2017

Glutamate-induced oxidative stress results in neuro-degenerative disorders in many central nervous system (CNS) such as Alzheimer's disease, ischemia, Huntington's disease, and Parkinson's disease. Our study was performed to investigate neuroprotective effects of Allium hookeri extracts (leaf, root, and whole) on glutamate-induced HT22 cells. In this study, ethanol extract of A. hookeri showed the outstanding neuroprotective effect in HT22 cells. In addition, we found that ethanol extract of A. hookeri root increased heme oxygenase (HO)-1 in HT22 cells. Moreover, ethanol extract of A. hookeri root also upregulated nuclear accumulation of nuclear factor E2-related factor 2 (Nrf2) in HT22 cells. These results demonstrate that ethanol extract of A. hookeri root contributes neuroprotective effects against glutamate-induced oxidative stress in HT22 cells, via Nrf2-mediated HO-1 expression. Our study suggests that ethanol extract of A. hookeri root could be the potential agent for the treatment of many neuro-degenerative diseases.

• KSBB Journal
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• 제4권3호
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• pp.288-292
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• 1989

인참 근 조직에 Agrobacterium rhizogenes strain $A_4$를 접종하여 hairy roots 유도와 유도된 hairy roots의 배양 조건을 조사하였다. 48시간 배양된 균($2{\times}A92$bacteria/ml)을 접종하여 암조건($26{pm}1^{\circ}C$)하에서 배양하였을때 6-7주후 tumor가 형성되었으며, 10-12주후 hairy roots가 유도되었다. 근 년별 hairy roots 유도율은 5년근이 4, 6년근에 비하여 높았으며, 배지에 IAA, 2, 4-D, IBA 및 tryptophan 을 각각 첨가시켰을때 15-30mg/l tryptophan에서 tumor 및 hairy roots 유도율이 증가되었음을 나타내었다. 또한 유도된 hairy roots hormone-free인 RCM배지(sucrose 3%, pH 4.5)에서 배양하였다.

• Safety and Health at Work
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• 제12권1호
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• pp.42-50
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• 2021

Introduction: Despite huge investments in new technology and transportation infrastructure, terrible accidents still remain a reality of traffic. Methods: Severe traffic accidents were analyzed from four prevailing modes of today's transportations: sea, air, railway, and road. Main root causes of all four accidents were defined with implementation of the approach, based on Flanagan's critical incident technique. In accordance with Molan's Availability Humanization model (AH model), possible preventive or humanization interventions were defined with the focus on technology, environment, organization, and human factors. Results: According to our analyses, there are significant similarities between accidents. Root causes of accidents, human behavioral patterns, and possible humanization measures were presented with rooted graphs. It is possible to create a generalized model graph, which is similar to rooted graphs, for identification of possible humanization measures, intended to prevent similar accidents in the future. Majority of proposed humanization interventions are focused on organization. Organizational interventions are effective in assurance of adequate and safe behavior. Conclusions: Formalization of root cause analysis with rooted graphs in a model offers possibility for implementation of presented methods in analysis of particular events. Implementation of proposed humanization measures in a particular analyzed situation is the basis for creation of safety culture.

• 한국작물학회지
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• 제29권1호
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• pp.39-45
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• 1984

본 실험은 수도의 유전연구에 전기영동법을 도입함에 있어 기초조사를 하기 위하여 수도 조직내의 몇가지 산소가 부위별로 차이가 있는지 또한 생장 조절제인 GA를 처이함으로 같은 산소의 동위효소 pattern이 변하는지 '아끼바레'와 '유신' 두 품종을 사용 조사하였다. 그 결과는 다음과 같았다. 1. Isoelectrofocusing에 의한 종자, Shoot, Root의 Esterase, Phosphatase, Amylase 동위효소 pattern은 두 품종 모두 조직부위별로 다른 pattern을 보였다. 2. 7％ polyacrglamide slab gel electrophoresis에 의한 Peroxidase 동위효소 pattern도 두 품종 모두 조직부위별로 다른 Pattern을 보였다. 3. GA(10/sup -5/)을 처리했을 경우 Esterase 동위효소 pattern은 '아끼바레'의 root와 '유신'의 shoot, root에서 대조구와 차이가 있었으며 Phosphatase의 동위효소 pattern은 '아끼바레'의 root에서 차이가 있었다. Amylase와 Peroxidase의 동위효소 pattern은 대조구와 GA 처리간에 차이가 없었다.

• Restorative Dentistry and Endodontics
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• 제21권1호
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• pp.267-279
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• 1996

Microorganisms are implicated the endodontic treatment failures. Persistent endodontic infection may be the result of retention of microorganisms in the dentin of the root canal walls. Dentinal tubules of the root canal walls have been shown to harbor microorganisms. The purpose of this study was to investigate the invasion of microorganism into the root dentin and dentinal tubules. The effects of irrigation solutions and smear layer on bacterial colonization of root canal were evaluated using a scanning electron microscopy. Canals of extracted human teeth with single and straight canals were stepback prepared using normal saline. Tooth samples were divided into four groups according to the irrigation solutions -5 % sodium hypochlorite and normal saline-and smear layer treatment. The smear layer was removed by 5% NaOCl and 20% EDTA for 10 min respectively. After sterilization, they were incubated with each strains of Streptococcus sanguis, Enterococcus faecalis, Staphylococcus aureus and Escherichia coli. Sodium hypochlorite solution reduced the adhesion of microorganisms effectively compared to normal saline. The smear layer inhibited colonization of E. faecalis, S. aureus and E. coli in the root canals due to their blocking of dentianl tubules. But S. sanguis invaded dentinal tubules in the root canals without smear layer. It was suggested that bacterial attachment might be different according to the strains. Sodium hypochlorite inhibited bacterial attachment in the dentinal tubules dramatically. The absence or presence of smear layer affected bacterial invasion of the dentinal tubules.

• 한국식품영양학회지
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• 제32권2호
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• pp.138-147
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• 2019

In this study, we examined antioxidative effects and the anti-adipogenesis effect of different parts of Cudrania tricuspidata (C), and Morus alba (M). Total polyphenol contents were highest in M-root ($34.56{\pm}0.045mg\;GAE/g$), and there was no significant difference, between C-root and M-leaf. Total flavonoid contents of C-root were highest ($23.07{\pm}0.004mg\;QE/g$). To examine antioxidant activities of C and M extracts, DPPH and ABTS radical scavenging activity, and FRAP assay, was used. Results show that antioxidant activities of C and M extracts increased, in a dose-dependent manner. Adipocytes are generated by preadipocyte differentiation, during adipogenesis. Matured adipocytes accumulate in abnormal and cause obesity. We investigated effects of leaf and root extracts of C and M, on lipid accumulation, in 3T3-L1 adipocytes. Changes in cell morphology, and degrees of lipid accumulation in adipocytes, were evaluated by Oil Red O staining. Root extracts of C and M, reduced lipid content in a dose-dependent manner. Therefore, root extracts of C and M, may be good candidates for managing obesity.

• 대한수학회논문집
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• 제24권3호
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• pp.451-458
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• 2009

The set of root classes plays a crucial role in the Nielsen root theory. Extending Brown et al.'s work on the set of root classes of iterates of maps, we rearrange it into the reduced orbit set and show that under suitable hypotheses, any reduced orbit has the full depth property as in the Nielsen type theory of periodic orbits.