• Journal of Korean Neurosurgical Society
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• 제42권3호
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• pp.205-210
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• 2007

Objective : Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels mediate the hyperpolarization-activated currents (Ih) that participate in regulating neuronal membrane potential and contribute critically to pacemaker activity, promoting synchronization of neuronal networks. However, distinct regional and cellular localization of HCN channels in the brain have not been precisely defined. Aim of this study was to verify the precise cellular location of HCN1 channels in rat cerebellum to better understand the physiological role these channels play in synaptic transmission between CNS neurons. Methods : HCN1 expression in rat brain was analyzed using immunohistochemistry and electron-microscopic observations. Postsynaptic density-95 (PSD-95), otherwise known as locating and clustering protein, was also examined to clarify its role in the subcellular location of HCN1 channels. In addition, to presume the binding of HCN1 channels with PSD-95, putative binding motifs in these channels were investigated using software-searching method. Results : HCN1 channels were locally distributed at the presynaptic terminal of basket cell and exactly corresponded with the location of PSD-95. Moreover, nine putative SH3 domain of PSD-95 binding motifs were discovered in HCN1 channels from motif analysis. Conclusion : Distinct localization of HCN1 channels in rat cerebellum is possible, especially when analyzed in conjunction with the SH3 domain of PSD-95. Considering that HCN1 channels contribute to spontaneous rhythmic action potentials, it is suggested that HCN1 channels located at the presynaptic terminal of neurons may play an important role in synaptic plasticity.

• Mycobiology
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• 제42권2호
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• pp.152-157
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• 2014

The iron uptake and utilization pathways play a critical role in allowing human pathogens, including Cryptococcus neoformans, the causative agent of fatal meningoencephalitis, to survive within the mammalian body by competing with the host for iron. Here we show that the iron regulon is also required for diverse environmental stress responses and that in C. neoformans, it is regulated by the high-osmolarity glycerol response (HOG) pathway. Between CFO1 and CFO2, two ferroxidase genes in the iron regulon, CFO1 but not CFO2 was induced during oxidative and osmotic stress. Interestingly, we found that the HOG pathway repressed basal expression of both CFO1 and CFO2. Furthermore, when the HOG pathway was blocked, CFO2 also responded to oxidative and osmotic stress and the response of CFO1 was increased. We also established that CFO1 plays a major role in responding and adapting to diverse environmental stresses, including oxidative and genotoxic damage, osmotic fluctuations, heavy metal stress, and stress induced by cell membrane destabilizers. Therefore, our findings indicate that in C. neoformans, the iron uptake and utilization pathways are not only required for iron acquisition and survival, but also play a significant role in the environmental stress response through crosstalk with the HOG pathway.

• Journal of Korean Dental Science
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• 제8권2호
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• pp.74-81
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• 2015

Purpose: In osteoclast differentiation, actin-rich membrane protrusions play a crucial role in cell adhesion. Odontogenic ameloblast-associated protein (Odam) contributes to cell adhesion by inducing actin rearrangement. Odam-mediated RhoA activity may play a significant role in multinucleation of osteoclasts. However, the precise function of Odam in osteoclast cell adhesion and differentiation remains largely unknown. Here, we identify a critical role for Odam in inducing osteoclast adhesion and differentiation. Materials and Methods: The expression of Odam in osteoclasts was evaluated by immunohistochemistry. Primary mouse bone marrow and RAW264.7 cells were used to test the cell adhesion and actin ring formation induced by Odam. Result: Odam was expressed in osteoclasts around alveolar bone. Odam transfection induced actin filament rearrangement and cell adhesion compared with the control or collagen groups. Overexpression of Odam promoted actin stress fiber remodeling and cell adhesion, resulting in increased osteoclast fusion. Conclusion: These results suggest that Odam expression in primary mouse osteoclasts and RAW264.7 cells promotes their adhesion, resulting in the induction of osteoclast differentiation.

• 부모자녀건강학회지
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• 제19권1호
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• pp.25-32
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• 2016

Purpose: The purpose of this study was to use the social network analysis to verify the relationship between the interpersonal disposition and interactive characteristics of nursing college students. Five classes, a total of 117 nursing students were included in the study. Methods: The study interaction network centralization, density and in-degree & out-degree centrality were used as indicators for measuring students' study interaction. Also, the students' interpersonal disposition was measured using verified tool. Results: The results indicated that the ordinary type which gave and took the information generally showed the highest proportions in the study interaction network. Also, the empathic-acceptable tendency of social relation domain showed the highest proportions in the interpersonal disposition. Moreover, the students who play the isolate and the carrier role in the study interaction network had no relation with the expression tendency, and the transmitter role play students had no relation with the role tendency. Conclusion: Based on the results, the tutors should consider the students' interpersonal relationship disposition when implementing the instructional strategy in PBL curriculum.

• 아동학회지
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• 제32권2호
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• pp.105-121
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• 2011

The main purpose of this study was to examine the relationship between mothers' parenting, children's self-esteem, and stress. The participants were composed of 320 elementary school 5th graders (161 boys and 159 girls) from the Seoul area. They completed questionnaires on mothers' parenting, children's self-esteem, and stress. The data were analyzed by means of Pearson's correlation coefficients and regressions. It was observed that maternal warmth was negatively correlated with children's stress in both boys and girls. Children's self-esteem was also negatively correlated with children's stress in both boys and girls. in addition maternal warmth was positively correlated with self-esteem in both boys and girls. It was futher found that self-esteem tended to play a partially mediating role between maternal warmth and children's stress only in boys. Self-esteem tended to play a perfectly mediating role between maternal permissiveness/nonintervention and children's stress only in girls. These results indicated that children's self-esteem plays a crucial role in improving the levels of children's stress.

• 아동학회지
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• 제32권2호
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• pp.123-148
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• 2011

The purpose of this study was to explore children's experiences in a series of creative drama activities based around the use of picture books. The subjects were a total of 17 children 4 years of age. The research, implemented over the courses of 10 weeks, was integrated into their daily education curriculum. The children's drama activities were collected by participant observation, field notes, audio and videotape transcriptions, documentation of the young children's drama activities (eg. pantomimes, role plays & improvised expression, improvisations). The analysis reveals the following findings : 1) even shy and passive young children tried to participate in pantomime activities after reading books. 2) In role play & improvised expression activities, young children were immersed in the improvised situation and spontaneously expressed their emotions and thoughts as actors without the use of any scripts, in an impromptu manner. 3) In improvisation, young children collaborated with other children to create the improvisation by extending or transforming the content of the picture books through their own ideas.

• 한국게임학회 논문지
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• 제20권2호
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• pp.101-112
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• 2020

본 연구는 <오버워치>를 통해 고수준의 플레이어가 되는 과정에서 게임 내 협력을 위한 희생이 어떻게 수행되는가에 대해 살펴보았으며 주요 결과는 다음과 같다. 첫째, 낮은 경쟁전 점수대에서는 칭찬카드가 공격형-딜러와 지원형-힐러간에 차이가 크게 나타났으며 지원형에게 많은 칭찬카드가 부여되었다. 둘째, 플레이어의 점수가 높아질수록 힐러로부터 딜러에게로 칭찬카드가 더 많이 부여되었다. 셋째, 고점수대의 플레이어들이 힐러역할을 자발적으로 맡음으로써 '자애로운 역할'을 자임하며 팀의 규범을 선도했고 결과적으로 팀 내에서 발생할 수 있는 역할 갈등의 위험을 분산했다. 이로부터 점수대가 높을수록 협력이 강해짐을 확인하였다.

• 의학교육논단
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• 제13권1호
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• pp.35-43
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• 2011

Purpose: Communication skills are considered as one of the essential requirements in medical education, and the training often involves exercising medical interviews with standardized patients(SP) or role play. This study investigated the helpfulness of the communication skills training programs for students' performance on the clinical performance examination (CPX). Methods: Fourth-year students who have taken one of two communication skills programs(exercising communication skills with SP or elective communication skill course with role play) completed a questionnaire on their evaluation of the helpfulness of communication skills programs immediately after finishing the CPX. Then, the programs were objectively assessed by comparing all fourth-year students' CPX scores between program participants and non-participants. Results: About 70% of participants answered that the programs were helpful to perform clinical clerkship and CPX. However, there was no difference in either the total CPX score or 2 categorical scores(integrated clinical encounter, communication and interpersonal skill) between program participants and non-participants. Conclusion: Although the students felt that the communication skills programs were helpful to their clinical activities, this study failed to find objective evidence of any effect of the programs on the CPX results. Communication skills training should be continued during clinical clerkship to maintain or enhance the skills, and it is necessary to introduce more effective methods for precise evaluation of students' communication skills.

• 한국한의학연구원논문집
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• 제15권3호
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• pp.53-61
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• 2009

Oxytocin(OT), classically known to stimulate labour and milk ejection, contributes to play an important role in a wide range of behavioral effects including drug addiction. An increasing body of evidence suggests that OT ameliorates acute and long-term effects of commonly used drugs by means of interacting with the mesolimbic dopamine system. Mesolimbic dopamine system is thought to play a major role in the reinforcing properties of drug abuse. Oxytocin receptors in the nucleus accumbens(NAc) and ventral tegmental area(VTA) have been implicated in the regulation of reinforcing effects in abused drugs. In the same way acupuncture may attenuate the reinforcing effects of abused drugs in the NAc and VTA. We have an interest in similar liaison between the substrates of acupuncture and drug addiction that may involve OT. Here, we described the possibility that acupuncture modulates the reinforcing and sensitizing properties of abused drugs in the dopaminergic system via the regulation of activities in the oxytocinergic system. The elements in this paper are summarized as follows : neuroanatomical studies of oxytocinergic innervation and distribution of oxytocin receptors; experiments related to the methamphetamine, cocaine, morphine and ethanol; experiments related to the oxytocin and acupuncture.

• 유통과학연구
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• 제22권3호
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• pp.49-58
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• 2024

Purpose: Research on financial development plays a crucial role in guiding and implementing policies for both financial development and economic growth. This study aims to evaluate the impact of financial development on the economic growth of Southeast Asian countries. Research design, data and methodology: The research utilizes data from 11 Southeast Asian countries from 2015 to 2022. Financial development data is proxied by credit distribution in private sector. Results: Based on the analysis using the FGLS model, it indicates that financial development has a positive impact on the economic growth of Southeast Asian countries. In addition, the study also examines the impact of state investment costs and FDI investment on economic growth. The results also show that foreign direct investment flows still play an important role in Southeast Asian countries (FDI has a positive impact on economic growth). State investment costs also impact economic growth, showing that the development of public investment also brings good development to countries. Conclusions: These results suggest that credit policies for financial development in general, and the development of private credit in particular, play a significant role in these countries. Building a system to promote the activities of private sector economies will help stimulate the economic development of Southeast Asian countries.