Journal of Korean Dental Science

Korean Academy of Dental Science (대한치의학회)
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Odontogenic Ameloblast-Associated Protein (Odam) Plays Crucial Roles in Osteoclast Differentiation via Control of Actin Ring Formation

  • Lee, Hye-Kyung (Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Park, Joo-Cheol (Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University)
  • Received : 2015.09.24
  • Accepted : 2015.12.19
  • Published : 2015.12.30
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Abstract

Purpose: In osteoclast differentiation, actin-rich membrane protrusions play a crucial role in cell adhesion. Odontogenic ameloblast-associated protein (Odam) contributes to cell adhesion by inducing actin rearrangement. Odam-mediated RhoA activity may play a significant role in multinucleation of osteoclasts. However, the precise function of Odam in osteoclast cell adhesion and differentiation remains largely unknown. Here, we identify a critical role for Odam in inducing osteoclast adhesion and differentiation. Materials and Methods: The expression of Odam in osteoclasts was evaluated by immunohistochemistry. Primary mouse bone marrow and RAW264.7 cells were used to test the cell adhesion and actin ring formation induced by Odam. Result: Odam was expressed in osteoclasts around alveolar bone. Odam transfection induced actin filament rearrangement and cell adhesion compared with the control or collagen groups. Overexpression of Odam promoted actin stress fiber remodeling and cell adhesion, resulting in increased osteoclast fusion. Conclusion: These results suggest that Odam expression in primary mouse osteoclasts and RAW264.7 cells promotes their adhesion, resulting in the induction of osteoclast differentiation.

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References

  1. Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature. 2003; 423: 337-42. https://doi.org/10.1038/nature01658
  2. Teitelbaum SL, Ross FP. Genetic regulation of osteoclast development and function. Nat Rev Genet. 2003; 4: 638-49. https://doi.org/10.1038/nrg1122
  3. Väänänen HK, Zhao H, Mulari M, Halleen JM. The cell biology of osteoclast function. J Cell Sci. 2000; 113: 377-81.
  4. Teitelbaum SL. Bone resorption by osteoclasts. Science. 2000; 289: 1504-8. https://doi.org/10.1126/science.289.5484.1504
  5. Chellaiah MA, Soga N, Swanson S, McAllister S, Alvarez U, Wang D, Dowdy SF, Hruska KA. Rho-A is critical for osteoclast podosome organization, motility, and bone resorption. J Biol Chem. 2000; 275: 11993-2002. https://doi.org/10.1074/jbc.275.16.11993
  6. Chellaiah MA. Regulation of actin ring formation by rho GTPases in osteoclasts. J Biol Chem. 2005; 280: 32930-43. https://doi.org/10.1074/jbc.M500154200
  7. Teti A, Marchisio PC, Zallone AZ. Clear zone in osteoclast function: role of podosomes in regulation of bone-resorbing activity. Am J Physiol. 1991; 261: C1-7. https://doi.org/10.1152/ajpcell.1991.261.1.C1
  8. Lee HK, Ji S, Park SJ, Choung HW, Choi Y, Lee HJ, Park SY, Park JC. Odontogenic ameloblastassociated protein (ODAM) mediates junctional epithelium attachment to teeth via integrin- ODAM-Rho guanine nucleotide exchange factor 5 (ARHGEF5)-RhoA signaling. J Biol Chem. 2015; 290: 14740-53. https://doi.org/10.1074/jbc.M115.648022
  9. Berdeaux RL, Díaz B, Kim L, Martin GS. Active Rho is localized to podosomes induced by oncogenic Src and is required for their assembly and function. J Cell Biol. 2004; 166: 317-23. https://doi.org/10.1083/jcb.200312168
  10. Hall A. Rho GTPases and the actin cytoskeleton. Science. 1998; 279: 509-14. https://doi.org/10.1126/science.279.5350.509
  11. Lee HK, Lee DS, Ryoo HM, Park JT, Park SJ, Bae HS, Cho MI, Park JC. The odontogenic ameloblastassociated protein (ODAM) cooperates with RUNX2 and modulates enamel mineralization via regulation of MMP-20. J Cell Biochem. 2010; 111: 755-67. https://doi.org/10.1002/jcb.22766
  12. Kestler DP, Foster JS, Bruker CT, Prenshaw JW, Kennel SJ, Wall JS, Weiss DT, Solomon A. ODAM expression inhibits human breast cancer tumorigenesis. Breast Cancer (Auckl). 2011; 5: 73-85.
  13. Moffatt P, Smith CE, St-Arnaud R, Nanci A. Characterization of Apin, a secreted protein highly expressed in tooth-associated epithelia. J Cell Biochem. 2008; 103: 941-56. https://doi.org/10.1002/jcb.21465
  14. Lee HK, Park SJ, Oh HJ, Kim JW, Bae HS, Park JC. Expression pattern, subcellular localization, and functional implications of ODAM in ameloblasts, odontoblasts, osteoblasts, and various cancer cells. Gene Expr Patterns. 2012; 12: 102-8. https://doi.org/10.1016/j.gep.2012.02.002
  15. Park JC, Park JT, Son HH, Kim HJ, Jeong MJ, Lee CS, Dey R, Cho MI. The amyloid protein APin is highly expressed during enamel mineralization and maturation in rat incisors. Eur J Oral Sci. 2007; 115: 153-60. https://doi.org/10.1111/j.1600-0722.2007.00435.x
  16. Lee HK, Park JT, Cho YS, Bae HS, Cho MI, Park JC. Odontogenic ameloblasts-associated protein (ODAM), via phosphorylation by bone morphogenetic protein receptor type IB (BMPR-IB), is implicated in ameloblast differentiation. J Cell Biochem. 2012; 113: 1754-65.
  17. Wang Q, Xie Y, Du QS, Wu XJ, Feng X, Mei L, McDonald JM, Xiong WC. Regulation of the formation of osteoclastic actin rings by proline-rich tyrosine kinase 2 interacting with gelsolin. J Cell Biol. 2003; 160: 565-75. https://doi.org/10.1083/jcb.200207036
  18. Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol. 2014; 15: 178-96. https://doi.org/10.1038/nrm3758
  19. Ne RF, Witherspoon DE, Gutmann JL. Tooth resorption. Quintessence Int. 1999; 30: 9-25.
  20. Kamat M, Puranik R, Vanaki S, Kamat S. An insight into the regulatory mechanisms of cells involved in resorption of dental hard tissues. J Oral Maxillofac Pathol. 2013; 17: 228-33. https://doi.org/10.4103/0973-029X.119736
  21. Teitelbaum SL. Osteoclasts: what do they do and how do they do it? Am J Pathol. 2007; 170: 427-35. https://doi.org/10.2353/ajpath.2007.060834
  22. Davidovitch Z. Tooth movement. Crit Rev Oral Biol Med. 1991; 2: 411-50. https://doi.org/10.1177/10454411910020040101
  23. Lekic P, McCulloch CA. Periodontal ligament cell population: the central role of fibroblasts in creating a unique tissue. Anat Rec. 1996; 245: 327-41. https://doi.org/10.1002/(SICI)1097-0185(199606)245:2<327::AID-AR15>3.0.CO;2-R
  24. Roberts WE, Goodwin WC Jr, Heiner SR. Cellular response to orthodontic force. Dent Clin North Am. 1981; 25: 3-17.
  25. Harokopakis-Hajishengallis E. Physiologic root resorption in primary teeth: molecular and histological events. J Oral Sci. 2007; 49: 1-12. https://doi.org/10.2334/josnusd.49.1
  26. Hasegawa T, Kikuiri T, Takeyama S, Yoshimura Y, Mitome M, Oguchi H, Shirakawa T. Human periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitro. Tissue Cell. 2002; 34: 44-51. https://doi.org/10.1054/tice.2002.0223
  27. Becktor KB, Nolting D, Becktor JP, Kjaer I. Immunohistochemical localization of epithelial rests of Malassez in human periodontal membrane. Eur J Orthod. 2007; 29: 350-3. https://doi.org/10.1093/ejo/cjm023
  28. Rincon JC, Young WG, Bartold PM. The epithelial cell rests of Malassez: a role in periodontal regeneration? J Periodontal Res. 2006; 41: 245-52. https://doi.org/10.1111/j.1600-0765.2006.00880.x
  29. Nishio C, Wazen R, Kuroda S, Moffatt P, Nanci A. Expression pattern of odontogenic ameloblastassociated and amelotin during formation and regeneration of the junctional epithelium. Eur Cell Mater. 2010; 20: 393-402. https://doi.org/10.22203/eCM.v020a32
  30. Nishio C, Wazen R, Kuroda S, Moffatt P, Nanci A. Disruption of periodontal integrity induces expression of apin by epithelial cell rests of Malassez. J Periodontal Res. 2010; 45: 709-13. https://doi.org/10.1111/j.1600-0765.2010.01288.x
  31. Sabag N, Mery C, García M, Vasquez V, Cueto V. Epithelial reattachment after gingivectomy in the rat. J Periodontol. 1984; 55: 135-41. https://doi.org/10.1902/jop.1984.55.3.135
  32. Nakaya H, Kamoi K. Immunohistological study of wound healing in periodontal tissue of rats. Distribution of fibronectin and laminin after flap operation. Nihon Shishubyo Gakkai Kaishi. 1989; 31: 462-90. https://doi.org/10.2329/perio.31.462
  33. Caffesse RG, Quinones CR. Polypeptide growth factors and attachment proteins in periodontal wound healing and regeneration. Periodontol 2000. 1993; 1: 69-79. https://doi.org/10.1111/j.1600-0757.1993.tb00208.x
  34. Dean JW 3rd, Blankenship JA. Migration of gingival fibroblasts on fibronectin and laminin. J Periodontol. 1997; 68: 750-7. https://doi.org/10.1902/jop.1997.68.8.750
  35. Kim JY, Kim BI, Jue SS, Park JH, Shin JW. Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats. Angle Orthod. 2012; 82: 107-14. https://doi.org/10.2319/030911-173.1