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Screening of melon genotypes identifies gummy stem blight resistance associated with Gsb1 resistant loci

  • Hassan, Md Zahid;Robin, Arif Hasan Khan;Rahim, Md Abdur;Natarajan, Sathishkumar;Kim, Hoy-Taek;Park, Jong-In;Nou, Ill-Sup
    • Journal of Plant Biotechnology
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    • v.45 no.3
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    • pp.217-227
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    • 2018
  • Gummy stem blight (GSB) is one of the most destructive and economically important, soil borne diseases of melon caused by the ascomycete fungus, Didymella bryoniae throughout the world. In Korea, however, no GSB resistant genotype has been reported yet. The study aimed to identify GSB resistant melon germplasm. We screened a total of 60 genotypes including 16 lines and 44 melon cultivars collected from USA and Korea. Among the 16 melon lines, four lines including 'PI482399', 'PI140471', 'PI136170' and 'PI420145', and two Korean cultivars viz. 'Asia Papaya' and 'Supra' showed complete resistance. We were aware that both genotypic and environmental variations could influence the phenotypic screening of resistance and susceptibility. We therefore, further assessed all genotypes using 20 SSR markers. The SSR marker 'CMCT505' linked to Gsb1 in chromosome 1 perfectly grouped resistant and susceptible lines indicating that resistance is probably due to the presence of Gsb1 gene. Cloning and sequencing of resistant and susceptible Gsb1 amplicons showed that there were 32-bp deletions in resistant line and 39-bp deletions in resistant cultivar compared to susceptible one. Thus, the resistant melon lines and cultivars identified in this study could be recommended for the melon breeding program. Furthermore, the SSR marker 'CMCT505' which is tightly linked with Gsb1 could be used for molecular screening of melon germplasm.

Motivating Underserved Vietnamese Americans to Obtain Colorectal Cancer Screening: Evaluation of a Culturally Tailored DVD Intervention

  • Lee, Hee Yun;Tran, Marie;Jin, Seok Won;Bliss, Robin;Yeazel, Mark
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.4
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    • pp.1791-1796
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    • 2014
  • Background: Colorectal cancer (CRC) is a leading cause of cancer death among Vietnamese Americans, yet screening remains underutilized. We investigated the effectiveness of a culturally tailored DVD intervention in promoting CRC screening among unscreened Vietnamese Americans age 50 and over. Materials and Methods: Using a community-based participatory research approach, we conducted a trial comparing twenty-eight subjects who received a mailed DVD in Vietnamese, with twenty-eight subjects who received a mailed brochure in Vietnamese. Subjects completed telephone surveys at baseline, One-month, and one-year. The primary outcome was receipt of screening. Secondary measures were participants' knowledge, attitudes, and beliefs about CRC screening. Two focus groups explored the intervention's acceptability and effectiveness. Results: At one year, CRC screening rates of 57.1% and 42.9% were observed in experimental and control group respectively (p=0.42), Subjects in both groups showed increased knowledge about CRC after one month. Focus group findings revealed that the DVD was an effective method of communicating information and would help promote screening. Conclusions: The findings suggest that culturally tailored, linguistically appropriate content is more important than the type of media used. This relatively low intensity, low cost intervention utilizing a DVD can be another useful method for outreach to the often hard-to-reach unscreened population.

A Proposal for Improving Techniques of GTS Utilization Based on WBAN (WBAN 기반의 GTS 채널 이용률 향상기법 제안)

  • Park, Joo-Hee;Jung, Won-Soo
    • Journal of the Korea Society of Computer and Information
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    • v.16 no.10
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    • pp.73-81
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    • 2011
  • The WBAN(Wireless Body Area Network) technology is a short distance wireless network which provides each device's interactive communication by connecting devices inside and outside of body located within 3 meters. Standardization on the physical layer, data link layer, network layer and application layer is in progress by IEEE 802.15.6 TG BAN. The The WBAN servides consists of both medical and non-medical applications. The medical application service uses the sensor that transfer the periodic traffic and have different data rates. It uses GTS method to guarantee QoS. In this paper, a new method is proposed, which are suitable design for MAC Protocol. Firstly, MAC frame structure and a primitive based on the WBAN are proposed. Secondly, we proposed the GTS algorithm improved the channel utilization based on the WFQ(Weighted Fair Queuing). The proposed scheduling method is improved channel utilization compared with i-Game(Round Robin scheduling method).

Blood and Body Fluid Exposure Related Knowledge, Attitude and Practices of Hospital Based Health Care Providers in United Arab Emirates

  • Zaidi, Moazzam Ali;Grifftths, Robin;Beshyah, Salem A.;Myers, Julie;Zaidi, Mukarram A.
    • Safety and Health at Work
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    • v.3 no.3
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    • pp.209-215
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    • 2012
  • Objectives: Knowledge, attitudes, and practices of healthcare providers related to occupational exposure to bloodborne pathogens were assessed in a tertiary-care hospital in Middle East. Methods: A cross-sectional study was undertaken using a self-administered questionnaire based on 3 paired (infectivity known vs. not known-suspected) case studies. Only 17 out of 230 respondents had an exposure in the 12 months prior to the survey and of these, only 2 had complied fully with the hospital's exposure reporting policy. Results: In the paired case studies, the theoretical responses of participating health professionals showed a greater preference for initiating self-directed treatment with antivirals or immunisation rather than complying with the hospital protocol, when the patient was known to be infected. The differences in practice when exposed to a patient with suspected blood pathogens compared to patient known to be infected was statistically significant (p < 0.001) in all 3 paired cases. Failure to test an infected patient's blood meant that an adequate risk assessment and appropriate secondary prevention could not be performed, and reflected the unwillingness to report the occupational exposure. Conclusion: Therefore, the study demonstrated that healthcare providers opted to treat themselves when exposed to patient with infectious disease, rather than comply with the hospital reporting and assessment protocol.

Synchronized sensing for wireless monitoring of large structures

  • Kim, Robin E.;Li, Jian;Spencer, Billie F. Jr;Nagayama, Tomonori;Mechitov, Kirill A.
    • Smart Structures and Systems
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    • v.18 no.5
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    • pp.885-909
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    • 2016
  • Advances in low-cost wireless sensing have made instrumentation of large civil infrastructure systems with dense arrays of wireless sensors possible. A critical issue with regard to effective use of the information harvested from these sensors is synchronized sensing. Although a number of synchronization methods have been developed, most provide only clock synchronization. Synchronized sensing requires not only clock synchronization among wireless nodes, but also synchronization of the data. Existing synchronization protocols are generally limited to networks of modest size in which all sensor nodes are within a limited distance from a central base station. The scale of civil infrastructure is often too large to be covered by a single wireless sensor network. Multiple independent networks have been installed, and post-facto synchronization schemes have been developed and applied with some success. In this paper, we present a new approach to achieving synchronized sensing among multiple networks using the Pulse-Per-Second signals from low-cost GPS receivers. The method is implemented and verified on the Imote2 sensor platform using TinyOS to achieve $50{\mu}s$ synchronization accuracy of the measured data for multiple networks. These results demonstrate that the proposed approach is highly-scalable, realizing precise synchronized sensing that is necessary for effective structural health monitoring.

A Striping Technique for Multi-Resolution of the MPEG-1 Video Stream (MPEG-1 비디오 스트림의 다중 해상도를 위한 스트라이핑 기법)

  • 김진환
    • Journal of Korea Multimedia Society
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    • v.6 no.5
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    • pp.769-777
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    • 2003
  • We present a striping technique that MPEG-1 video streams ell a disk array can be efficiently played back at different resolution levels. For the MPEG-1 compression algorithm, the proposed multi-level encoding technique first partitions the parent video stream in the temporal dimension. Each frame in the sub-stream is then Partitioned in the chroma dimension yielding a low resolution and a residual component. The multimedia server stores blocks of different components on consecutive disks in a round robin manner. As a result, the lower the resolution level being maintained, the smaller is the number of disks accessed by each client. To effectively utilize a disk array and to maximize the number of clients that can be serviced simultaneously, the proposed technique interleaves the storage of the component of sub-streams among the disks in the array We empirically validate and evaluate this striping technique through simulation in order to show the improvement of its performance on the server.

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A Hidden-Node-Aware Grouping Algorithm for Improving Throughput of IEEE 802.15.4 (IEEE 802.15.4의 성능 향상을 위한 은닉 노드 인식 그룹핑 알고리즘)

  • Um, Jin-Yeong;Ahn, Jong-Suk;Lee, Kang-Woo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.8A
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    • pp.702-711
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    • 2011
  • This paper proposes a HAG(Hidden-Node-Aware Grouping) algorithm for IEEE 802.15.4 networks to enhance the performance by eliminating collisions resulted from the hidden node problem without adopting the RTS/CTS packet exchanges. To solve the hidden node problem, the HAG algorithm organizes nodes into disjoint transmission groups by dynamically allocating hidden nodes into separate groups which take turns in a round robin way for their transmission. For dynamic group adjustment, it periodically evaluates the presence of hidden nodes based on subordinate nodes' receipt reports. To accurately measure its behavior, this paper also builds an analytical model to estimate its throughput fluctuation over various network topologies. The mathematical model along with simulation results confirmed that the HAG technique gracefully degraded the throughput of IEEE 802.15.4 networks whereas the standard IEEE 802.15.4 networks suffer severe throughput fallout as hidden nodes become populated.

Quantitative Surface Analysis of Co-Ni and Au-Cu alloys by XPS and SIMS (XPS와 SIMS에 의한 Co-Ni과 Au-Cu 합금표면 정량분석 연구)

  • 김경중;문대원;이광우
    • Journal of the Korean Vacuum Society
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    • v.1 no.1
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    • pp.106-114
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    • 1992
  • Abstract-Quantitative surface analysis of Co-Ni and Au-Cu alloys by XPS and SIMS was studied. For Co-Ni alloy, quantitative XPS analysis could be done within 1-2% relative error with pure element standards without any correction. For Au-Cu, quantitative XPS analysis was not possible without any correction. But it could be done with standard alloys of various composition within 1-2% relative error. Without standard alloys, Au-Cu alloys could be analyzed by XPS within 10% relative error with pure element standards. For SIMS analysis of Co-Ni alloys, the relative secondary ion yields of Co+/Nit has linear relation with ratio of each composition so that quantitative SIMS analysis was possible for Co-Ni alloys. Preliminary results of XPS round robin test of VAMAS-SCA Japan Project are given.

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Measurement of Traceability Error for Calibration Service Center Using Type S Thermocouples (S형 열전대를 이용한 교정검사기관의 소급성 오차측정)

  • Gam, Kee-Sool;Kim, Yong-Gyoo;Kim, Sun-Gon
    • Journal of Sensor Science and Technology
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    • v.4 no.1
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    • pp.43-50
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    • 1995
  • We measured the traceability error for nine high temperature calibration service centers including KRISS through the round-robin test. In this test the type S thermocouple, which used as a calibration standard thermometer commonly, was accommodated as a test thermocouple. Intercomparison data of three institutions were coincident with KRISS's data within ${\pm}0.5^{\circ}C$, which was the calibration uncertainty of the type S thermocouple, but the remaining six institution's data were deviated from the assigned uncertainty level. Deviation of the intercomparison data increased gradually according to the increase of the test temperature. and the maximum difference was so large as about $2.0^{\circ}C$ at the highest test temperature, gold point. In this study we found the traceability error of high temperature calibration service center for a high temperature standard was within $2.0^{\circ}C$.

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Protective Effect of Herbal Mixture Including Lycii Fructus on Hepatotoxicity Induced by Thioacetamide in Mice (구기자 복합물 약침액이 간기능 개선에 미치는 영향)

  • Kim, Yong-Min;Hwang, Dong-Suk;Kwak, Byeong-Mun;Kim, Ee-Hwa
    • Korean Journal of Acupuncture
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    • v.36 no.4
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    • pp.221-229
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    • 2019
  • Objectives : This study investigated the hepatoprotective effect of herbal mixture including Lycii fructus (HML) in thioacetamide (TAA)-induced hepatotoxicity in mice. Methods : To confirm the liver protective effect, induced by TAA for 3 days injection at 100 mg/kg mice, HML were treated for 8 weeks at 300 mg/kg/day, 1000 mg/kg/day. Positive control was treated silymarin 50 mg/kg/day after TAA injection. The changes of mortality rate, clinical signs, organ weight, relative liver, blood chemistry and histopathological findings were analyzed after experiment. Results : Body weight gain was observed in all groups, but TAA treated group at 4th week and all treated groups decreased weight compared to the untreated group. As a result of organ weight measurement, organ weight gain due to hepatic injury was observed statistically significantly in TAA-treated group and TAA+Silymarin treated group, and the herbal mixture-treated group showed a tendency to decrease compared to the TAA treated group. Blood biochemistry showed that total cholesterol and very low density lipoprotein cholesterol decreased statistically in TAA+low-dose and high dose herbal mixture treated group compared to the TAA-treated group. Histopathological examination showed that liver abnormalities were not observed in untreated group, liver fibrosis was observed in liver injury with TAA treated and herbal mixture treated group. And, TAA+high dose herbal mixture group showed relaxation tendency on liver calcification compared to the TAA treated group. Conclusions : According to the above results, HML provided hepatoprotective effects on the hepatic injury by reduction of inflammatory responses.