• 한국분무공학회지
• /
• 제25권2호
• /
• pp.60-67
• /
• 2020

Despite its benefit in engine thermal efficiency, gasoline-direct-injection (GDI) engines generate substantial particulate matter (PM) emissions compared to conventional port-fuel-injection (PFI) engines. One of the reasons for this is that the spray collapse caused by the spray-to-spray interaction forms the locally rich fuel-air mixture and increases the fuel wall film. Previous studies have investigated the spray collapse phenomenon through the macroscopic observation of spray behavior using laser optical techniques, but it is somewhat difficult to understand the interaction between sprays that is initiated in the near-nozzle region within 10 mm from the nozzle exit. In this study, the spray structure, droplet size and velocity data were obtained using an X-ray imaging technique from the near-nozzle to the downstream of the spray to investigate the spray-to-spray interaction and discuss the effects of spray collapse on local droplet size and velocity distribution. It was found that as the ambient density increases, the spray collapse was promoted due to the intensified spray-to-spray interaction, thereby increasing the local droplet size and velocity from the near-nozzle region as a result of droplet collision/coalescence.

• 한국자기공명학회논문지
• /
• 제22권3호
• /
• pp.64-70
• /
• 2018

Human Tid1, belonging to the family of the Hsp40/DnaJ, functions as a co-chaperone of cytosolic and mitochondrial Hsp70 proteins. In addition, the conserved J-domain and G/F-rich region of Tid1 has been suggested to interact with the p53 tumor suppressor protein, to translocate it to the mitochondria. Here, backbone NMR assignments were achieved for the putative p53-binding domain of Tid1. The obtained chemical shift information identified five ${\alpha}$-helices including four helices characteristic of J-domain, which are connected to a short ${\alpha}$-helix in the G/F-rich region via a flexible loop region. We expect that this structural information would contribute to our progressing studies to elucidate atomic structure and molecular interaction of the domain with p53.

• 한국생물정보학회:학술대회논문집
• /
• 한국생물정보시스템생물학회 2004년도 The 3rd Annual Conference for The Korean Society for Bioinformatics Association of Asian Societies for Bioinformatics 2004 Symposium
• /
• pp.77-85
• /
• 2004

A significant portion (about 8% in human genome) of mammalian mRNA sequences contains AU(Adenine and Uracil) rich elements or AREs at their 3' untranslated regions (UTR). These mRNA sequences are usually stable. ARE motifs are assorted into three classes. The importance of AREs in biology is that they make certain mRNA unstable. We analyzed the occurrences of AREs and Alu, and propose a possible mechanism on how human mRNA could acquire and keep A REs at its 3' UTR originated from Alu repeats. Interspersed in the human genome, Alu repeats occupy 5% of the 3' UTR of mRNA sequences. Alu has poly-adenine (poly-A) regions at the end that lead to poly -thymine (poly-T) regions at the end of its complementary Alu. It has been discovered that AREs are present at the poly -T regions. In the all ARE's classes, 27-40% of ARE repeats were found in the poly -T region of Alu with mismatch allowed within 10% of ARE's length from the 3' UTRs of the NCBI's reference m RNA sequence database. We report that Alu, which has been reported as a junk DNA element, is a source of AREs. We found that one third of AREs were derived from the poly -T regions of the complementary Alu.

• BMB Reports
• /
• 제43권3호
• /
• pp.199-204
• /
• 2010

The protein p104 was first isolated as a binding partner of the Src homology domain of phospholipase C$\gamma$1, and has been shown to associate with p85$\alpha$, Grb2. The ectopic expression of p104 reduced cellular growth rate, which was also achieved with the overexpression of only the proline-rich region of p104. The proline-rich region of p104 has been found to inhibit the colony formation of platelet-derived growth factor BB-stimulated NIH3T3 cells and MCF7 cancer cells on soft agar. Mutagenesis analysis showed that the second and third proline-rich regions are essential for growth control, as well as for interaction with p85$\alpha$. Overexpression of p104 increased the level of the cyclin-dependent kinase inhibitor, $p27^{Kip1}$, and inhibited the activity of phosphoinositide 3-kinase (PI3K). In summary, p104 interacts with p85$\alpha$ and is involved in the regulation of $p27^{Kip1}$ expression for the reduction of cellular proliferation.

• Journal of Magnetics
• /
• 제22권2호
• /
• pp.244-249
• /
• 2017

To get deeper insight into the effect of intergranular additives in sintered Nd-Fe-B magnet and consequently improve the properties better, the interaction between additives (oxide, nitride, and carbide) and Nd-rich phase in the temperature range of 298.15-1400 K was analyzed thermodynamically. It can be found that the oxide additives became less stable than nitrides and carbides. Except for calcium oxide, almost all oxides could react with Nd from Nd-rich phase. To be different from oxide additives, the mechanism of nitrides and carbides was defined with various elements, either reaction with Nd from Nd-rich phase or not. The two different mechanisms would show different effects on the microstructure and hence properties of magnet. The thermodynamic analysis had a better agreement with the experimental information.

• 대한화학회지
• /
• 제38권12호
• /
• pp.857-863
• /
• 1994

Sodium dodecyl sulfate (SDS) 존재하에서 유기색소인 thionine (TH)의 흡수 및 형광분광학적 성질을 조사 연구하였다. SDS 농도 증가에 따라 측정한 흡수 및 형광스펙트럼의 변화들로부터 i) TH-SDS 착화합물의 회합체 형성, ii)TH-SDS 회합체내의 색소분자의 stacking 과정, iii)dye-rich induced micelles의 형성에 의한 staking의 파괴 및 iv) SDS 미셀의 표면에 TH의 재분포과정 등으로 설명하였다.

• BMB Reports
• /
• 제32권4호
• /
• pp.405-408
• /
• 1999

The transactivation potential of HIV-1 Vpu was identified from the yeast two-hybrid screening process. The helix II domain of HIV-1 Vpu protein and mutant Vpu protein lacking the transmembrane domain exhibited transactivation of the LacZ and Leu2 reporter genes carrying LexA upstream activating sequences, but full-length HIV-1 Vpu and the helix I domain of HIV-1 Vpu did not. The helix II domain of HIV-1 Vpu consists of a number of acidic amino acids, and is especially rich in glutamic acid, a characteristic of many transcription factors. This result suggests that protein-protein interaction may occur through the acidic helix II domain of HIV-1 Vpu.

• Applied Microscopy
• /
• 제47권4호
• /
• pp.233-237
• /
• 2017

Chronic eosinophilic leukemia (CEL) is a myeloproliferative disease with an increased number of mature eosinophils and their precursors, which results in infiltration into organs and organ enlargement. The main cause of this disease is the overexpression of tyrosine kinase. However, there is a need for alternative medication, because some patients are resistant to imatinib, which is a tyrosine kinase inhibitor for leukemia. Many studies have indicated that S100A8 and splicing factor proline and glutamine-rich (SFPQ) function as initiation signals of apoptosis in CEL cells. We reviewed structural studies on CEL cells related to S100A8 and SFPQ. Particularly, this review highlighted microscopic results for the study of S100A8 and SFPQ in CEL cells.

• 한국공간정보시스템학회 논문지
• /
• 제11권4호
• /
• pp.56-64
• /
• 2009

최근 웹 2.0 기술의 활용이 증가하면서 RIA (Rich Internet Application) 기술에 대한 중요성도 증가하고 있다. RIA는 인터넷 서비스가 가능한 데스크탑 및 모바일 기기에서 다양한 정보를 제공할 수 있는 장점이 있다. 본 논문에서는 RIA 기술 중에서 플래시를 사용하여 공간 데이터를 모바일 환경에서 서비스하는 방법을 제안한다. 공간 데이터 서버는 사용자가 요구한 공간 데이터를 RIA 기술 중 하나인 플래시 기반의 웹 어플리케이션에 포함하여 제공하는 방법을 사용한다. 본 논문에서 제안하는 방법은 클라이언트에서 렌더링 및 사용자 인터페이스 처리를 담당하는 특징을 가진다. 또한, 서버로부터 웹 어플리케이션을 다운로드 받으면 추가로 공간 데이터 서버에 접속 없이도 클라이언트 자체에서 공간 데이터의 패닝, 확대/축소 등의 처리가 가능하므로 무선 언터넷 환경을 사용하는 모바일 장치에서 효과적이다. 그리고, 풀랫폼에 독립적으로 동작되어 모바일 환경에서도 데스크탑 환경과 동일하게 활용할 수 있는 장점이 있다. 본 논문에서 제안하는 공간 데이터 서버의 성능을 평가하기 위하여 WMS(Web Map Server)와 비교 분석한다.

• 대한공업교육학회지
• /
• 제33권1호
• /
• pp.213-232
• /
• 2008

이 연구의 목적은 멀티미디어 기술을 활용한 프로젝트 학습전략을 개발하고, 수업현장에 적용하여 그 효과에 대한 학습자들이 인식과 시사점을 제시하는 것이다. 이 연구는 J기계공업고등학교 환경에서 시행된 현장연구이다. 연구 대상은 3학년 100명을 대상으로 하였다. 자료는 관찰, 설문조사, 인터뷰를 통하여 수집되었고, 백분율로 처리 되었다. 결론적으로 멀티미디어 기술을 활용한 프로젝트 학습 효과로서 전공교과내용의 종합적 이해, 컴퓨터 멀티미디어 처리능력과 자료 탐색 및 조직 능력이 향상된 것으로 학습자들은 인식하였다. 또한, 학습 동기, 학습자들 간의 협동 활동, 학습 참여 정도, 학습의 만족감, 학습에 대한 자신감, 학습자들 간의 상호작용과 학습 흥미 등이 높아진 것으로 학습자들은 인식하고 있음을 알 수 있었다. 마지막으로 연구의 시사점과 제언을 제시하였다.