• Bulletin of the Korean Chemical Society
• /
• 제32권10호
• /
• pp.3756-3759
• /
• 2011

• Bulletin of the Korean Chemical Society
• /
• 제30권3호
• /
• pp.739-741
• /
• 2009

• Journal of Ginseng Research
• /
• 제11권1호
• /
• pp.77-83
• /
• 1987

인삼사포닌이 벼(Oryza sativa L.)의 초기생장 및 발아에 미치는 영향을 조사하기 위하여, 볍씨를 $10^{-4}$% 사포닌용액에 침종시킨 후, ${\circ}C$에서 성장시켰다. 인삼사포닌용액($10^{-4}$%)에 60시간 동안 침종한 볍씨(시험군)는 증류수에 침종한 대조군에 비해 시간에 따른 생장이 촉진(약 10%)되었으며, 잎의 출현시기도 통계적으로 빨랐다. 또한, 볍씨 발아시의 주요효소인 amylase의 활성을 12, 24, 48, 72시간 침종한 볍씨파쇄액에서 조사한 결과, 시험군이 대조군에 비해 각각 1.27, 1.58, 1.69, 1.49배 촉진되었으며, in viro 실험의 결과도 $10^{-5}$%사포닌 존재하에서 대조군에 비해 활성이 약 30% 증가하였다. 이상의 결과로 부터 인삼사포닌의 벼의 발아에 기능하는 amylase를 활성화하여, 벼의 발아 및 초기생장을 촉진하는 것으로 해석된다.

• Journal of Microbiology and Biotechnology
• /
• 제17권2호
• /
• pp.271-279
• /
• 2007

Black rice (Oryza sativa L. var. japonica) has been used in folk medicine in Asia. To understand the effects of black rice hydrolyzed peptides (BRP) from germinated black rice, we assessed the expression levels of about 20,000 transcripts in BRP-treated HaCaT keratinocytes using human 1A oligo microarray analysis. As a result, the BRP treatment showed a differential expression ratio of more than 2-fold: 745 were activated and 1,011 were repressed. One of the most interesting findings was a 2-fold increase in hyaluronan synthase 2 (HAS2) gene expression by BRP. Semiquantitative RT-PCR showed that BRP increased HAS2 mRNA in dose-dependent manners. ELISA showed that BRP effectively increased hyaluronan (HA) production in HaCaT keratinocytes.

• 대한화학회지
• /
• 제54권1호
• /
• pp.65-70
• /
• 2010

유색미인 흑남벼(Oryza sativa cv. Heugnambyeo) 미강의 ethylacetate 가용성 용매분획물로부터 NO 생성을 저해시키는 단일화합물을 순수 분리한 후, HREI-MS 및 2D-NMR 분석을 통해 화학구조를 4-carboethoxy-6-methoxy-2-quinolone (1)과 4-carboethoxy-6-hydroxy-2-quinolone (2)으로 명명하였다.

• Molecules and Cells
• /
• 제20권1호
• /
• pp.119-126
• /
• 2005

${\alpha}$-Expansins are bound to the cell wall of plants and can be solubilized with an extraction buffer containing 1 M NaCl. Localization of ${\alpha}$-expansins in the cell wall was confirmed by immunogold labeling and electron microscopy. The subcellular localization of vegetative ${\beta}$-expansins has not yet been studied. Using antibodies specific for OsEXPB3, a vegetative ${\beta}$-expansin of rice (Oryza sativa L.), we found that OsEXPB3 is tightly bound to the cell wall and, unlike ${\alpha}$-expansins, cannot be solubilized with extraction buffer containing 1 M NaCl. OsEXPB3 protein could only be extracted with buffer containing SDS. The subcellular localization of the OsEXPB3 protein was confirmed by immunogold labeling and electron microscopy. Gold particles were mainly distributed over the primary cell walls. Immunohistochemistry showed that OsEXPB3 is present in all regions of the coleoptile and root tissues tested.

• Preventive Nutrition and Food Science
• /
• 제7권2호
• /
• pp.119-122
• /
• 2002

Oryza sativa cv. Mihyangbyo is one of several recently developed varieties of rice; characterized by high levels of aromatic components, which may increase its sensory and nutritional properties. In conjunction with our continuing investigation of bioactive components of improved grain varieties, a quinolone alkaloid was isolated from the n-butanol soluble fraction of the aleurone layer of Oryza sativa cv. Mihyangbyo (Gramineae) through activity-guided fractionation and isolation. The compound exhibited moderate antineoplastic activity in a human leukemia cell line (U937) with an $IC_{50}$/ value of 118.1 ug/mL, based on the MTT(3-[4, 5]dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) cell proliferation assay. The chemical structure of the functional compound was determined, based on physical and spectroscopic characteristics.

• Preventive Nutrition and Food Science
• /
• 제3권2호
• /
• pp.198-202
• /
• 1998

The arbitrary primed polymerase chain reaction (AP-PCR) has been used to detect the genetic alternations in the related species. Simple and reproducible fingerprints of complex genomes can be generated using single arbitrary chosen primers and the PCR. The technique was applied to the Oryza species and characterized the relationship among three cultivars of rice species based on theresult of genomic DNA fingerprints. The results indicated that the polymorphism revealed in rice strains and the differences in the PCR product pattern could be represented for each strainis. There was many variationsin the PCR product pattern between cv. Dongin(japonica type)and cv.Hyangdo (indica type), and our chosen AP-primers can ge as markers for strain identification and verfication.

• Genomics & Informatics
• /
• 제5권4호
• /
• pp.194-195
• /
• 2007

We have constructed the Rice Genome Information Service System (RGISS), which is an information service system of the Oryza sativa L. ssp. japonica (rice) genome, using the released version of rice Build 3.0 pseudomolecules based on the Ensembl architecture. The nonredundant library, composed of 3,360 clones of BACs, PACs, and fosmids, was used to construct supercontigs. RGISS contains 50,717 annotated genes from GenBank, 56,161 predicted genes from FgeneSH, and information on 9,587 markers, which includes STS, SSR, and EST-based RFLP. The 20,180 ESTs sequenced by the Korea National Institute of Agricultural Biotechnology (NIAB) were aligned and mapped into 168,792 exons. By gene ontology analysis, the classified protein numbers in the rice genome were 6158, 4531, and 12,364 proteins, which were mapped to molecular function, cellular component, and biological process, respectively.

• Journal of Applied Biological Chemistry
• /
• 제58권4호
• /
• pp.355-361
• /
• 2015

The rice protein profiles of Oryza sativa L (Koshihikari) grown under organic and conventional cultivation regimes were compared on 2-D gels to develop diagnostic marker proteins for organic rice. The selected proteins, differentially expressed between organic and conventional rice, were compared with the differentially expressed proteins of another organic and conventional rice pairing, produced at a different location. In the first comparison among conventional, no-chemical, and organic rice grown in the same region, Korea, 13 proteins exhibiting differential expression in organic and conventionally grown plants were selected. Eight of the 13 proteins were down-regulated, and the 5 remaining proteins were up-regulated from conventional to organic rice. The second comparison pairing from Kyungju, revealed 12 differentially expressed proteins, with 8 down-regulated and 4 up-regulated proteins. Ten of the differentially expressed proteins that overlapped between the two comparison sets could not be clustered into any functional group using a functional annotation clustering tool. Further comparisons using another set of conventional and organic rice, belonging to a different variety of Oryza sativa L and produced in Sanchung, revealed 8 differentially expressed proteins, 5 of which were down-regulated and 3 of which were upregulated in the organic rice. Overall, 3 differentially expressed proteins were commonly found in all three organic rice crops. These 3 proteins, along with other overlapping differentially expressed proteins, can provide a good starting point for the development of signature proteins that can be used for the authentication of organic rice with a follow-up studies with more comparison sets.