• Journal of Nutrition and Health
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• v.26 no.6
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• pp.680-691
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• 1993

Rats were fed for an 8-week period a low riboflavin diet(5ug riboflavin/day) or a control diet(30ug/day) supplied either ad libitum or by pair feeding in order to study the effect of riboflavin on the metabolism of lipids and neurotransmitters. Erythrocyte glutathione reductase (EGR) and monomine oxidase(MAO) activity in the liver and brain were assayed. EGR activity coefficient in riboflavin deficient rats was significantly higher than in ad libitum controls whereas MAO activity was decreased in the deficient rats. Fatty acid composition showed a different trend in the serum, liver and brain. In the serum, the concentrations of essential fatty acids and $\omega$-3 fatty acids(eicosapentaenoic acid, docosahexaenoic acid)were decreased about 20-40% in the deficient and pair-fed than in the ad libitum controls. Brain serotonin and 5-HIAA(5-hydroxyindole acetic acid) concentrations were decreased in the riboflavin deficient rats. Learning ability measured by a water maze and exploratory activity using the open field test were not impaired in the deficient rats. These results indicate that brain lipid metabolism was protected in subclinical riboflavin deficiency, however, riboflavin deficiency affected brain serotonin content.

• Journal of Nutrition and Health
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• v.25 no.2
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• pp.150-161
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• 1992

The purpose of this study was to evaluate the riboflavin status of primary school children. Fiftyone subjects were selected as obese group and fiftyfive subjects were selected as control group according to Body Mass Index(BMI) of fifth-graders at a primary school in Taegu. For each subject information on nutrient intake and daily activity pattern were obtained by questionnaire. The riboflavin status was evaluated by urinary riboflavin exvretion The daily energy expenditure per kilogram of body weight was significantly lower in obese group(=47kcal/day) than in control group(=58kcal/day) (p<0.001) However the entire energy consumption was siginificantly greater in the obese children(=2005kcal/day) than their nono-baser peers(=1837kcal/day)(p<0.001). Riboflavin intake was 0.67mg/100kcal in the control group and 0.61mg/1000kcal in the obese group. Thus intakes for both groups met the current group and control group were 86.9$\mu\textrm{g}$/day and 98.7$\mu\textrm{g}$/day. repectively. There was no significnat Assesment of clinical signs of riboflavin deficiency indicated that angular lesion was 4.7% and glossitis was 6.6% of all subjects. Thirty one percent of subjects excrete riboflavin below 78$\mu\textrm{g}$/g creatinine which is defined as deficient. Therefore this group would be considered at high risk for developing riboflavin deficiency. From this study current recommendation of 0.6mg/1000kcal of riboflavin intake may not be adequate during growth and associated stress.

• The Korean Journal of Zoology
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• v.38 no.4
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• pp.498-504
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• 1995

The effeds of riboflavin defidency on the biosynthesis of flavin pepddes and levels of flavoenzymes and catecholamines have been investigated. The percentage of 14C. riboflavin radioactivity formed in mitochondria appeared to increase up to 2 weeks but started to decline at 3 weeks. A significant increase of radioactivity incorporation into mitochondria and into trypsin-digestable plus trypsin-non-digestibie flavin peptides was detected in riboflavin-deficient animals. More than 35% of incorporation was observed at the end of the first week and 160% higher incorporation was observed in fiavin peptide after the second week. Activities of MAO and succinate dehydrogenase were affected markedly by riboflavin status whereas those of acetyichoilnesterase were not affected. Riboflavin defidency also brought about marked reductions in levels of epineplrrine and norepinephrine. it is concluded that the levels of flavin peptides, MAO and succinate dehydrogenase, and catecholamines were affected significanily by the availability of riboflavin and in particular the duration of its depiction.

• BMB Reports
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• v.42 no.9
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• pp.568-573
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• 2009

Fatty acid oxidation (FAO) defects cause abnormal lipid accumulation in various tissues, which provides an opportunity to uncover novel genes that are involved in lipid metabolism. During a gene expression study in the riboflavin deficient induced FAO disorder in the chicken, we discovered the dramatic increase in mRNA levels of an uncharacterized gene, ANKRD9. No functions have been ascribed to ANKRD9 and its orthologs, although their sequences are well conserved among vertebrates. To provide insight into the function of ANKRD9, the expression of ANKRD9 mRNA in lipidperturbed paradigms was examined. The hepatic mRNA level of ANKRD9 was repressed by thyroid hormone ($T_3$) and fasting, elevated by re-feeding upon fasting. However, ANKRD9 mRNA level is reduced in response to apoptosis. Transient transfection assay with green fluorescent protein tagged- ANKRD9 showed that this protein is localized within the cytoplasm. These findings point to the possibility that ANKRD9 is involved in intracellular lipid accumulation.

• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.665-672
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• 2004

D-Ribose is a five-carbon sugar used for the commercial synthesis of riboflavin, antiviral agents, and flavor enhancers. Batch fermentations with transketolase-deficient B. subtilis JY1 were carried out to optimize the production of D-ribose from xylose. The best results for the fermentation were obtained with a temperature of $37^{\circ}C$ and an initial pH of 7.0. Among various sugars and sugar alcohols tested, glucose and sucrose were found to be the most effective for both cell growth and D-ribose production. The addition of 15 g/l xylose and 15 g/l glucose improved the fermentation performance, presumably due to the adequate supply of ATP in the xylose metabolism from D-xylulose to D-xylulose-5-phosphate. A batch culture in a 3.7-1 jar fermentor with 14.9 g/l xylose and 13.1 g/l glucose resulted in 10.1 g/l D-ribose concentration with a yield of 0.62 g D-ribose/g sugar consumed, and 0.25 g/l-h of productivity. Furthermore, the sugar utilization profile, indicating the simultaneous consumption of xylose and glucose, and respiratory parameters for the glucose and sucrose media suggested that the transketolase-deficient B. subtilis JY1 lost the glucose-specific enzyme II of the phosphoenolpyruvate transferase system.

• Journal of Community Nutrition
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• v.6 no.2
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• pp.67-77
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• 2004

In order to investigate the differences of nutrient intakes by the economic status and different age groups and to identify the nutritional risk group and its specific nutrition problem, 2001 Korean National Health and Nutrition Survey were analyzed. The subject's numbers of 9,391 were classified into four classes such as low (14.2%), medium (37.2%), high (26.0%), and high above (22.6%) on the basis of the family monthly income and the 2001 Korean minimum cost of living according to the family size. Mean intakes of energy and all nutrients assessed by the RDAs, lipid-energy %, and MAR were increased as the economic status were going up. Na intake expressed per 1,000kcal was in reverse. Nearly a half(45.5%) of the low-income people seemed to take nutritionally inadequate diet in consideration with MAR values. Deficiencies of iron and even energy in the toddlers (1 to 2 years) of low-income class were of great concern. Adolescent age group has been observed that their calcium and iron intakes, and possibly energy, were appeared to be the most deficient among all the age groups regardless of the economic status. For the elderly in all the economic status except high-above class, calcium, vitamin A, and riboflavin were commonly deficient nutrients. Calcium deficiency was appeared throughout nearly all the ages except toddlers and all the economic classes. Even in the high-above class 57.3% took insufficient amount of calcium.

• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.560-564
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• 2013

${\small{D}}$-Ribose is a value-added five-carbon sugar used for riboflavin production. To investigate the effects of oxygen supply and mixed sugar concentration on microbial production of ${\small{D}}$-ribose, a transketolase-deficient Bacillus subtilis SPK1 was cultured batch-wise using xylose and glucose. A change of agitation speed from 300 rpm to 600 rpm at 1 vvm of air supply increased both the xylose consumption rate and ${\small{D}}$-ribose production rate. Because the sum of the specific consumption rates for xylose and glucose was similar at all agitation speeds, metabolic preferences between xylose and glucose might depend on oxygen supply. Although B. subtilis SPK1 can take up xylose and glucose by the active transport mechanism, a high initial concentration of xylose and glucose was not beneficial for high ${\small{D}}$-ribose production.

• Korean Journal of Community Nutrition
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• v.11 no.1
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• pp.39-51
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• 2006

This study was conducted to investigate the differences in daily nutrient intakes, dietary habits and nutrition knowledge between male and female college students. Male undergraduate students 004 subjects) and female undergraduate students (229 subjects) , enrolled at H University in Daejeon, were recruited for this study. The mean age of the subjects was 22.9 year in male and 20.4 year in female students. Daily intakes of energy and nutrients were calculated from the records of one day of dietary food intakes by 24-hour recall method, and general information, dietary habits and attitudes, food intake frequency and food preferences and knowledge for food and nutrition were surveyed through a questionnaire. About $70.1\%$ of male and $66.1\%$ of female students were in the normal range of BMI (18.5 - 23), and $25.2\%$ of male students were over-weighted in contrast to $27.7\%$ of female students were underweight. Males and females were taking $77.2\%$ and $77.9\%$ of RDA for energy, respectively, and $54.8\%$ of male and $48.0\%$ of female students were taking energy under $75\%$ of RDA. Many of them showed deficient intakes of calcium, iron, vitamin A and riboflavin. Average of MAR was 0.75 in male and 0.72 in female students. NAR for calcium, iron, vitamin A and riboflavin in male students were 0.55, 0.69,0.75 and 0.61, respectively, compared to 0.53, 0.51, 0.70 and 0.67 in female students. The nutrients, which have INQ less than 1, were calcium and riboflavin in male, compared to calcium, iron, and riboflavin in female students. There were no gender differences in meal regularity and meal skipping rates, but female students showed higher rates of skipping dinner than males (p < 0.001) About $50.8\%$ of female students were ingesting snacks 1 - 2 time/day, compared to $27.1\%$ of male students. For the food intake frequency, fruit group was significantly eating more for female than male students (p< 0.001) Though female students got higher scores for nutritional knowledge test (p < 0.001) than male students, they did not show better dietary habits or dietary attitudes than male students actually. Therefore, a more active and actual education program accustomed to the different genders and ages with focus on real changing of dietary behaviors needs to be developed and run in schools and local departments.

• Journal of Nutrition and Health
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• v.15 no.1
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• pp.70-79
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• 1982

Using dietary, anthropometric, and biochemical methods of evaluation, a nutritional survey was made of 81 seven-and eight-year-old children from two schools with different socioeconomic backgrounds in Daejon city, during the October of 1980. All the children were tall and heavy in comparison with 1976 Korean standard. School A children with relatively high socioeconomic levels showed higher value in length and lower value in weight than school B children with relatively low socioeconomic levels. Mean hemoglobin values were 13.3 (school A) and 12.8 (school B) g/100 ml and hematocrits were 39.7 and 37.9%, respectively. Anemic children were not many in both schools ( A ; 0-25%, B ; 12.5-15.0% ). Urea nitrogen/creatinine ratios were 9.2 (A) and 7.8 (B). The intake of animal foods was higher in school A owing to the higher intake of milks. The intakes of all nutrients except energy and calcium were comparable to or higher than Korean recommend dietary allowances (KRDA). Riboflavin intake wae deficient in school B only. Low socioeconomic school B children had significantly lower intakes of fats, calcium, and riboflavin than school A children. Energy intakes were low as 75.9 (A) and 83.2 (B) % of KRDA but their composition of carbohydrates, fats, and proteins were satisfactory in both schools. More than one-third of protein intake was animalorigin in both schools (A ; 1.40/3, B ; 1.10/3) and calcium intake was very low as 59.0%(A) and 45.8%(B). Education levels of parents, esp. mothers, were positively correlated with Kaup indice (in school A & B) and some nutrients intakes (in school A). In school B, number of siblings and birth order showed negative correlations with some nutrient intakes.

• Journal of the Korean Dietetic Association
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• v.7 no.4
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• pp.349-360
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• 2001

The purpose of this study was to investigate the nutritional status of school lunch supported students and to provide fundamental data for improving their health. In order to do that, we collected the data for 59 school lunch supported students and 71 not supported students in an elementary school in the Taegu Metropolitan city area. The survey was conducted from February 10 to April 30, 2000. The results are as follows; In relation to their body composition(BMI, Fatmass, Percent Body Fat, Mid-upper Arm Muscle Circumference, Mid-upper Arm Circumference), the school lunch supported students' value was lower than that of the not supported students. In relation to food intake according to the food groups, the school lunch supported students' intake of animal food(p<0.05) and total food(p<0.01)was significantly lower than that of the other students. The average intake of energy for both school lunch supported students and not supported students was below the RDA. Also, it was significantly lower than that of the school lunch supported students in relation to the other students(p<0.05). For protein, calcium, phosphorous, and riboflavin, the school lunch supported students' intake was significantly lower than that of the students who were not supported(p<0.05, p<0.05, p<0.05, p<0.05). There are the classification in relation to the nutrient intake in relation to the RDA standards for that of the school lunch supported students. The criteria used for this study was calorie intake, protein, and niacin. The classifications are for groups below 75%, between 75%～100%, and 100%. The nutrients for which the students were deficient( below 75%) of the RDA were iron, calcium, riboflavin, vitamin C, thiamin, calorie, niacin, vitamin A, protein, and phosphorous in the above order.