• Applied Biological Chemistry
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• v.39 no.6
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• pp.506-511
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• 1996

Six phenolic acids were isolated from Jindalrae flowers (Rhododendron mucronulatum Turcz.), an edible plant in Korea. These compounds were identified as chlorogenic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, caffeic acid, ferulic acid, and p-coumaric acid on the basis of IR, UV, $^{1}H$ and $^{13}C$ NMR, FAB-MS, ES-MS and/or El-MS data. Chlorogenic acid (0.2 g) present in both ethyl acetate and ethyl ether fractions comprised up to 38.5% of the total phenolic acid amount (0.52 g) finally recovered by means of polyamide C-200 column chromatography, preparative TLC, recrystallization, and Sephadex LH-20 column chromatography The antioxidant activities were measured in an ethanol solution of linoleic acid in the presence of ferric thiocyanate. The antioxidant efficiency increased in the order of p-coumaric acid<${\alpha}-tocopherol$

• Korean Journal of Agricultural Science
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• v.44 no.1
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• pp.104-113
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• 2017

Anthocyanins contained in Rhododendron schlippenbachii (royal azalea) are expressed in a variety of colors and affect flower colors. R. schlippenbachii flowers of seven colors (white, red group: pink, deep pink, red, purple group: light purple, purple, deep purple) were collected from the garden around KT&G building in the college of agriculture and life science. Seven types of anthocyanins [cyanidin 3-O-diglucoside, cyanidin 3-O-arabinoside-5-O-glucoside, cyanidin 3-O-galactoside, peonidin 3-O-arabinoside-5-O-glucoside, cyanidin 3-O-glucoside, cyanidin 3-O-(6"-O-malonyl) arabinoside, cyanidin 3-O-(6"-O-coumaroyl) glucoside] turned out to be from the cyanidin and peonidin series in R. schlippenbachii flowers. Also, seven types of flavonols [azaleatin 3-O-glucoside, azaleatin 3-O-arabinoside, azaleatin 3-O-rhamnoside, quercetin 3-O-galacatoside, quercetin 3-O-glucoside, quercetin 3-O-arabinoside, quercetin 3-O-rhamnoside] were identified in R. schlippenbachii flowers. Total anthocyanin amounts decreased in R. schlippenbachii flowers in the following order: 'deep pink' (8.07) > 'red' (6.37) > 'pink' (5.35) > 'deep purple' (0.78) > 'purple' (0.43) > 'light purple' ($0.22mg{\cdot}g^{-1}$ dry weight, DW) > 'white' (not detected). Total flavonol amounts decreased in the following order: 'pink' (97.78) > 'deep pink' (63.79) > 'deep purple' (61.98) > 'white' (57.58) > 'light purple' (47.06) > 'purple' (46.76) > 'red' ($7.60mg{\cdot}g^{-1}$ dry weight, DW). This study provided the quantitative and qualitative information for the variation of anthocyanin and flavonol compounds in R. schlippenbachii flowers. Furthermore, this information can contribute to the identification of anthocyanin and flavonol compounds in other Rhododendron flowers.

• Korean Journal of Environment and Ecology
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• v.15 no.4
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• pp.354-360
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• 2002

To investigate the structure and the conservation strategy of Natural forest at the ridge from Gittaebong to Cheongoksan area 34 plots(10m$\times$10m) were set up with random sampling method. Three groups(Quercus mongolica community, Quercus mongolica-Tilia amurensis community. Quercus mongolica-Fraxinus rhynchophylla community) were classified by cluster analysis. High positive correlations were proved between Quercus mongolica and Pinus densiflora; Pyrus pyrifolia and Viburnum sargentii, Euonymus sachalinensis; Fraxinus sieboldiana and Rhododendron schlippenbachii, Pinus densiflora; Symplocos chinensis for. Pilosa and corylus sieboldiana var. mandshurica, Rhus trichocarpa Lespedeza bicolor; Rhododendron schlippenbachii and Rhododendron mucronulatum; Weigela subsessilis and Euonymus sachalinensis, Rhus trichocarpa, Lespedeza bicolor; Rhus trichocarpa and Lespedeza bicolor and high negative corrlations were proved between Quercus mongolica and Tilia amurensis, Acer Pseudo-sieboldianum. Species diversity(H') of investigated area was 0.5981~0.8150.

• Korean Journal of Environment and Ecology
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• v.3 no.1
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• pp.1-15
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• 1989

To investigate the actual vegetation and the degree of green naturality(DGN) in Gaya Mountain National Park, forest communities were classified by phytosocialogical analysis according to physiognomy and Braun-Blanquet methods. Major forest communities were as follows: 1. Pinus densiflora community 1) P. densiflora-Rhododendron mucronulatum subcommunity 2) P. densiflora-Lespedeza maximowiczii subcommunity 3) P. densiflora-Sasa borealis subcommunity 4) P. densiflora-Carex siderosticta subcommunity 2. Quercus mongolica community group. 1) Q. mongolica-Rhododendron schlippenbachii community a) Typical subcommunity b) Vaccinium koreanum subcommunity 2) Q. mongolica-Sasa borealis community 3) Q. mongolica-Lespedeza maximowiczii community 3. Quercus serrata community 4. Carpinus laxiflora-Quercus serrata community 5. Rhododendron tschonoskii community 6. Lespedeza crytobotrya-Miscanthus sinensis var. purpurascens community The map of DGN was drawn according to the actual vegetaton map. The ratio of DGN 7 and 8 was 93.9% in Gaya Mountain National Park.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.2
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• pp.87-95
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• 2004

For the slope stability and revegetation of retaining wall, Eco-Stone was built beside a newly constructed road in August, 2002. Eco-Stone blocks were constructed in 4 different combinations of irrigation and soil types. Within the Eco-Stone, planted were 6 species such as Forsythia koreana, Rhododendron mucronulatum, Spiraea prunifolia var. simpliciflora, Rhododendron sp. Euonymus japonica, and Aster koraiensis. Shoot growth was greater on common soils than at a better soil treatment for Forsythia koreana(P<0.01) and Spiraea prunifolia var. simpliciflora(P<0.05), while there were no significant differences for the other species. Biomass increment of the planted species also did not show significant differences between irrigation types, except Rhododendron sp. and Aster koraiensis of which biomass was higher under irrigation than at no irrigation for common soils. Most of the planted individuals were alive, showing survival ratio of 90~97% with no significant differences among treatment blocks. These results imply that the Eco-Stone can be used economically for slope stability and revegetation instead of concrete blocks, without a specific soil and irrigation requirement.

• Microbiology and Biotechnology Letters
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• v.48 no.4
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• pp.471-479
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• 2020

The aim of this study was to investigate the anti-oxidant and anti-inflammatory activities of the Rhododendron weyrichii flower extract fermented using Shindari, a traditional Jeju barley Nuruk-based fermentation. In this study, we examined the antioxidant potential of R. weyrichii flower extracts (RF) and R. weyrichii flower extracts fermented with Nuruk or Shindari (RFFN or RFFS, respectively) using various in vitro antioxidant assays including DPPH and ABTS radical scavenging assays, total phenol content and FRAP assays. We also evaluated the anti-inflammatory activity of the RF and RFFS on murine RAW 264.7 cells. The anti-inflammatory activity was evaluated by treating the RAW 264.7 cells with various concentrations (6.25, 12.5, 25, and 50 ㎍/ml) of RF or RFFS. As a result, we observed that the ABTS radical scavenging activity and total phenol content of RFFS was higher than that of RF and RFFN. Additionally, lipopolysaccharide-induced nitric oxide (NO) production was significantly lower in RFFS-treated cells when compared to the LPS-treated control. In addition, RFFS-treated cells exhibited decreased expression of inducible NO synthase (iNOS) proteins and high-performance liquid chromatography (HPLC) fingerprinting showed that both the quercetin and quercetin glucoside (quercitrin and isoquercitrin) levels were affected by the fermentation process. In conclusion, our data suggests that traditional fermentation could be an important strategy in improving the biological properties of raw materials including their antioxidant and anti-inflammatory activities. Finally, RFFS may be a candidate for developing topical antioxidant and anti-inflammatory agents.

• Journal of Forest and Environmental Science
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• v.38 no.2
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• pp.110-121
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• 2022

Leaf functional traits widely have been used to understand the environmental controls of resource utilization strategy of plants along the environmental gradients. By using key leaf functional traits, we quantified the relationships between leaf traits and local climate throughout the distributional range of Rhododendron caucasicum Pall. in eastern and western Georgian mountains (the South Caucasus). Our results revealed, that all traits showed high levels of intraspecific variability across study locations and confirmed a strong phenotypic differentiation of leaf functional variation along the east-west longitudinal gradient in response to the local climate; out of the explored climatic variables, the moisture factors related to precipitation and number of precipitation and dry days for winter and growth seasons were more strongly related to leaf trait variation than the elevation and air temperature. Among studied leaf traits, the leaf specific area (SLA) showed the highest level of variability indicating the different resource utilization strategies of eastern and western-central Rh. caucasicum individuals. High SLA leaves for western-central Caucasian individuals work in relatively resource-rich environments (more humid in terms of precipitation amount and the number of precipitation days in winter) and could be explained by preferential allocation to photosynthesis and growth, while eastern Caucasian samples work in resource-poor environments (less humid in terms of precipitation amount and the number of precipitation days in winter) and the retention of captured resources is a higher priority appearing in a low SLA leaves. However, more evidence from a broader study of the species throughout its distribution range by including additional environmental factors and molecular markers are needed for firmer conclusions of intraspecific variability of Rh. caucasicum.

• Journal of Life Science
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• v.32 no.8
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• pp.622-632
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• 2022

The purpose of this study was to investigate activities as functional cosmetic materials, focusing on Rhododendron brachycarpum (RB) and Rhododendron fortunei (RF). The tyrosinase inhibitory effect, related to skin-whitening, was 32.6% and 39.3% respectively at the concentration of 1,000 ㎍/ml. The elastase inhibitory effect, related to skin anti-wrinkling activity, was 30% and 36.2% at 1,000 ㎍/ml concentration. Collagenase inhibitory activity showed 77.7%, and 80.2% respectively at 1,000 ㎍/ml concentration, which demonstrated excellent inhibitory activity. For a cell viability test, that measured on fibroblast cells by RB and RF extracts. Furthermore, the cell viability test showed 100.9% and 98.9% with cell viability at 100 ㎍/ml concentration in CCD-986Sk. The protein expression inhibitory effect of RB and RF extracts was measured by western blot at 25, 50, and 100 ㎍/ml concentrations, and the β-actin was used as a positive control. As a result, western blot of RB and RF extracts was measured by the expression inhibition rate of the MMP-1, MMP-2, MMP-3 protein, and was decreased by 67.2%, 65.5%, 13.6%, and 89.1%, 85.0%, and 62.7% at 100 ㎍/ml concentration. The mRNA expression inhibitory effect of RB and RF extracts was measured by RT-PCR at 25, 50, and 100 ㎍/ml concentrations, and the GAPDH was used as a positive control. According to the results of RT-PCR of RB and RF extracts, the expression inhibition rate of the MMP-1, MMP-2, and MMP-3 mRNA was decreased by 70.1%, 9.1%, 37.9%, and 38.2%, 8.3%, 57.3% at 100 ㎍/ml concentrations. So, RB and RF extracts showed the anti-wrinkle effectiveness as a functional cosmetic material.

• Journal of Life Science
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• v.28 no.8
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• pp.938-944
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• 2018

This study evaluated tyrosinase, elastase inhibitory, and antioxidant activities of fermented Rhododendron micranthum Turcz. extract using a lactic acid bacterium, Lactobacillus rhamnosus. The optimum conditions for fermentation of R. micranthum Turcz. extract were $37^{\circ}C$ and 3% R. micranthum Turcz. extract for 3 days based on the bacterial cell number, total phenolic compounds, DPPH radical scavenging activity, and tyrosinase and elastase inhibitory activity. After culture for 3 days using 3% R. micranthum Turcz. extract, the cell mass of L. rhamnosus reached $5.7{\times}10^9CFU/ml$. The results indicated that R. micranthum Turcz. extract can be used for industrial lactic acid bacteria culture. After fermentation under optimum conditions, the total content of phenolic compounds of the fermented R. micranthum Turcz. extract was 157 GAE mg/ml, and the $IC_{50s}$ of DPPH radical scavenging activity, ABTS radical scavenging activity, and tyrosinase and elastase inhibitory effects were 78.8, 79.8, 329.1, and $449.5{\mu}g/ml$, respectively. The fermented R. micranthum Turcz. extract exhibited 1.2-, 1.3-, 1.5-, 2.4-, and 5.6-fold higher total content of phenolic compounds, DPPH radical scavenging activity, ABTS radical scavenging activity, and tyrosinase and elastase inhibitory effects than the nonfermented R. micranthum Turcz. extract. These results indicated that fermented R. micranthum Turcz. extract using L. rhamnosus can be used for developing new natural functional ingredients for the health food or cosmetic industry.

• Korean Journal of Environmental Agriculture
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• v.19 no.3
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• pp.213-217
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• 2000

In order to select more proper plants for phytoextraction at the heavy metal polluted areas, 11 species of non-edible plants were cultivated at the cadmium(Cd) and Lead(Pb) treated soils and analyzed the content of the absorbed Cd and Pb in each part of plants. Plants include three fibers(Linum usitatissimum, Cannabis sativa, Gossypium spp.), three flowers(Calendula officinalis, Rhododendron lateritium, Portulaca grandiflora), and five trees(Pinus thunbergii, Magnolia kobus, $Populus\;nigra\;{\times}\;P.$ maximowiczii, Euonymus japonica, Fraxinus rhynchophylla). Yield of tree species were higher than that of fiber and flower species. Cd and Pb were highly accumulated in root rather than leaves and stems. The Cd content of plants was in the order Portulaca grandiflora > Calendula officinalis > Gossypium spp. > Linum usitatissimum, Pb was Cannabis sativa > Linum usitatissimum > Fraxinus rhynchophylla. Total absorbed Cd by each plant was in the order $Populus\;nigra\;{\times}\;P.$ maximowiczii > Euonymus japonica > Rhododendron lateritium, but Pb was $Populus\;nigra\;{\times}\;P.$ maximowiczii > Rhododendron lateritium > Euonymus japonica. Total absorbed Cd and Pb contents in plants were negatively correlated with the residual Cd and Pb in the treated soils. It was estimated that $Populus\;nigra\;{\times}\;P.$ maximowiczii, Euonymus japonica, Fraxinus rhynchophylla, and Rhododendron lateritium were the most effective species for phytoextraction in the polluted area considering yield and heavy metal uptake.