• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.4
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• pp.341-351
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• 2019

In this study, we investigated anti-oxidative and anti-bacterial constituents from Rhododendron weyrichii leaves. DPPH and ABTS⁺ radical scavenging activities were screened for the ethanol extract and solvent fractions. Potent scavenging activities were appeared from the extract, ethyl acetate (EtOAc) and n-butanol (BuOH) fractions. Upon the anti-bacterial tests using Staphylococcus epidermidis and Propionibacterium acnes, extract, n-hexane (Hex) and EtOAc fractions showed strong activities. To isolate the active constituents, the EtOAc fraction was further purified to afford five phytochemicals; ursolic acid (1), corosolic acid (2), asiatic acid (3), astragalin (4) and isoquercetin (5). All of the compounds 1-5 were isolated for the first time from this plant. Among the isolates, the compound 4 and 5 showed strong DPPH and ABTS⁺ radical scavenging activities. Also, compound 3 exhibited the most potent anti-bacterial activity. In addition, the content of astragalin isolated from this plant was determined by UPLC and the quantity was about 8.1 mg/g for the 70% ethanol extract and 34.8 mg/g for the EtOAc fraction. Based on these results, it is concluded that R. weyrichii extract could be potentially applicable as anti-oxidative and anti-bacterial ingredients in cosmetic formulations.

• Journal of Plant Biology
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• v.2 no.2
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• pp.1-21
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• 1959

1. The plant communities on Mt. Sulak, a typical alpine mountain in central Korea was researched from the standpoint of ecological survey. 2. The plant on this mountain can largely be classified into 2 categories, the warm zone (below 1100m) and the frigid zone. The former is again classified into a broad-leaved tree formation and a needle-leved tree formation, and the latter is calssified into a broad-leaved tree formation and needle-leaved tree formation, and shrub formation. 3. The broad-leaved tree formation in the warm zone can largely be classified into a Carpinus laxiflora-syringa Palibiniana var. kanibayashi association(1), a Carpinus erosa-Acer Pseudo-sieboldianun association(2), and a Quercus mongolica- Tripterygium Regelii association(3). 4. The needle-leaved tree formation in the warm zone can also largely ben classified into a Pinus densiflora- Miscanthus sinensis association(4), Abies holophylla- Tripterygium regelii association(5), Pinus kordiensis- Rhododendron schlippenbachii association(6) In the association(4), the canopy of the Pinus densiflora is gradually being occupied by the Quercus mongolica which is one kind of broad-leaved trees. 5. We can fully see a DryoPteris type of the flour layer plant type in the association (3) and also see a Sasamorpha type in the association (5). 6. The broad-leaved tree formation in the frigid zone can largely be classified into Quercus mongolica- Rhododendron schlippenbachii association (8). The constituents of tree and subtree layers in (7) and (8) associations are similar to those association (3), but the constituents of the shrub layer are different from those of associtation (3) due to the difference in height. 7. The needle-leaved formation in the frigid zone can largely be classified into Thuja koraiensisRodgersia podophylla var. viritis association (9) and Abies nephrolepis-Acer tschonoskii var. rubripes asscciation (10). The species of alpine plants or subalpine plants are gradually increased in this formation. 8. The shrub formation can also largely be classified into a Rhododendron mucronulatum- Patrinia saniculaefolia association (11) and Pinus pumila associatio (12). Association (12) has largely developed on the windy place. Association (12) was burned due to the bombing during the Korean war, but now we recoginized there occurred an invasion of Tripterygium Regelii in such a place. 9. The herb layer species which constitute the shrub formation are mostly alpine or subalpine plants of small size, and their kinds are also very few. 10. The growth of the Moss layer is especially good because of the varied conditions of the habitat. The kinds which can easily be seen are the genus of Thunidium, Haplocladium, Brachythecium, Macromitrium, Holonitrium, Atrichum, Schwstchkeopsis, Grimmia, Hedwigia, Rhynchostegium and Mnium. 11. The genus of the Sphagnum densly grows like a mat at the acid and moisturous place above 1100m. The authors should express their thanks to father. Yang Kisup, Dean of Catholic Medical College, Prof. Yoon Doksuen, from their valuable advice. Thanks arealso due to Prof. Numata, Chiba University, Prof. Takagi, Nagoya University and Prof. Ando, Hiroshima University in Japan for his kind help and identification of Bryophytes.

• Korean Journal of Plant Resources
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• v.33 no.2
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• pp.106-115
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• 2020

In order to find out the relationship between spring phytophenological index and temperature index for the past nine years (2010~2018), this study identified the relationship between temperature changes and trends in spring phytophenological index of sprouting, flowering and leaf unfolding of Pinus densiflora Siebold & Zucc., Larix kaempferi (Lamb.) Carrière, Quercus mongolica Fisch. ex Ledeb., Rhododendron mucronulatum Turcz., Lindera obtusiloba Blume and Acer pseudosieboldianum (Pax) Kom. in Daegu Arboretum, Palgong mt., Juwang mt. and Gaya mt. The change in temperature was caused by an increase in the monthly average temperature between March and April compared to February for nine years, and the average temperature of Daegu Arboretum and Palgong mt. were higher by region. The sprouting, flowering and leaf unfolding were the fastest of the Lindera obtusiloba and the slowest of the Pinus densiflora for each species, and the fastest plant season in Daegu arboretum came. SPI (Spring Phytophenological Index) tends to advance by -1.267~-6.151/9 years, with the largest Pinus densiflora (-6.151/9 years), with the lowest rate of change for Rhododendron mucronulatum (-1.267 days/9 years). Species which shows significant values in sprouting, flowering and leaf unfolding is Rhododendron mucronulatum and Pinus densiflora, which correlate with the mean temperature of January to March. As a result of checking the time series change of SPI, the change rate in the four regions was all negative and the phenolocal index was all accelerating. Among them, the rate of change was greater in inland areas such as Daegu arboretum, Palgong Mt. and Gaya mt. and the rate of change was slightly lower in the case of Juwang mt., which is somewhat distant.

• Korean Journal of Environment and Ecology
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• v.28 no.6
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• pp.725-740
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• 2014

To figure out the vegetation situation in the ridge of the Naknam-Jeongmaek, six intensive survey sites were selected considering environmental factors and human interferences, 132 quadrats (area $100m^2$) were installed to survey appearing species of trees and major environmental factors, and the characteristics of vegetation structures were analyzed. The surveyed plots were divided into six groups according to the analysis of classification by TWINSPAN; Quercus mongolica-Q. variabilis community, Pinus densiflora-Q. dentata community, Chamaecyparis obtusa community, Q. mongolica-P. densiflora community, P. densiflora-P. thunbergii community, P. koraiensis community, Rhododendron spp.-Lespedeza cyrtobotrya community. P. densiflora-Q. dentata community, Q. mongolica-P. densiflora community, and P. densiflora-P. thunbergii community are expected to be succeeded by deciduous oaks because the power of deciduous oaks is strong in their lower layer. C. obtusa community, P. densiflora community, and Rhododendron spp.-L.cyrtobotrya community are artificial forests that were artificially formed and are expected to be maintained in the current state for some time because the dominance value of planted species of trees is high. Most vegetations in Naknam-Jeongmaek were secondary forests or artificial forests formed for forest tree production and forestation for erosion control. In particular the top regions and hilly sections of the mountain were mostly dominated by deciduous oaks such as Q. mongolica, Q. variabilis showed some P. densiflora community competing with deciduous oaks. On the other hand, low sections and regions adjacent to the city showed severe artificial interference since exotic species such as P. thunbergiil, C. obtusa, P. koraiensis, and Rhododendron spp. were planted.

• Korean Journal of Environment and Ecology
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• v.22 no.2
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• pp.106-112
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• 2008

To investigate the vegetation structure of the mountain ridges ranging from Songchu to Dobong, this research set up 22 plots$(400m^2)$ as survey target areas. As a result of the analysis of woody plant cluster, it was classified as two groups-Quercus mongolica community and Quercus mongolica-Pinus densiflora community. Quercus mongolica was found as a mostly dominant woody plant species in the ridge areas from Songchu to Dobong, while Pinus densiflora, Quercus serrata and Quercus acutissima were mixed up partly in low altitudes. High negative correlations were shown between Quercus mongolica and Quercus serrata, Quercus serrata and Acer pseudosieboldianum, Pinus densiflora and Acer pseudosieboldianum, and relatively high positive correlations were found to exist between Quercus acutissima and Quercus serrata; Quercus mongolica and Rhododendron mucronulatum; Pinus rigid a and Rhododendron schlippenbachii; Sorbus alnifolia and Rhododendron mucronulatum; Pinus rigida and Pinus densiflora; Pinus densiflora and Robinia pseudoacacia; Acer pseudosieboldianum and Styrax obassia; Magnolia sieboldii and Symplocos chinensis. Species diversity index(H') by community at the surveyed areas stayed in the scope of $0.997\sim1.160$, which indexes showed nothing different from the vegetation structure of other national parks.

• The Korean Journal of Ecology
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• v.28 no.2
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• pp.69-77
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• 2005

The forest vegetation of Namhae-gun was investigated using the methodology of the Z.-M. school of phytosociology from March, 1998 to September, 2003. The forest vegetation was classified into 12 communities, 11 subcommunities, 5 afforestations as follows : Quercus mongolica community(typical subcommunity, Rhododendron schlippenbachii subcommunity), Ouercus serrata community(typical subcommunity, Acer pseudo-sieboldianum subcommunity), Ouercus variablis community(typical subcommunity, Platycarya strobilacea subcommunity), Quercus acutissima community, Carpinus laxiflora community, Carpinus coreans community, Styrax japonica community, Zelkova serrata community, Corylopsis coreana community, Pinus densiflora community(typical subcommunity, Rhododendron mucronulatum subcommunity, Carpinus laxiflora subcommunity), Pinus thunbergii community(typical subcommunity, Eurya Japonica subcommunity), Rhododendron yedoense var. poukhanense community, Chamaecyparis obtusa afforestation, Alnus firma afforestation, Pinus rigida afforestation, Robinia pseudo-acacia afforestation, Cryptomeria japonica afforestation. The soil pH of the study area was ranged from 4.2 to 5.4, and soil moisture content was strongly related to organic matter content. The Corylopsis coreana community contained the highest soil moisture content, whereas Pinus densiflora community contained the lowest one. The content of organic matter were $15.8\sim19.1%$ in the Quercus mongolica, Carpinus coreans and Ouercus serrata communities, $3.3\sim5.4%$ in the Quercus acutissima, Corylopsis coreana and Pinus thunbergii communities, and $2.8\sim5.6%$ in the Pinus rigida and Cryptomeria japonica afforestation. There is significant correlation among the contents of cations($K^+$, $Ca^{2+}$ and $Mg^{2+}$).

• Journal of Korean Society of Forest Science
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• v.103 no.1
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• pp.1-11
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• 2014

This study was conducted to investigate the changes understory vegetation composition (shrub and herb layers) in Mt. Gyebang as a northern-temperate deciduous broadleaf forest. Tracheophytes were 146 taxa, consisting 56 families, 93 genera, 124 species, 17 varieties, 3 forma, 2 sub-species and 1 unknown taxa in research subject area. As species area curve analysis, herbaceous layer and shrub species have been decreased over time. As a result of Mantel-test, basal area of upper layer affects to understory vegetation change (p<0.0001). Mean importance value was dominated Lindera obtusiloba (21.585%), Rhododendron schlippenbachii (19.774%) in the shrub layer, identified Sasa borealis (14.082%) and Lindera obtusiloba (7.921%) in the herb layer. According to NMS analysis of shrub layer, Species characterized by strong correlation have been reduced as time goes by. And it reports different species as an increasing in basal area of the upper layer. Herb layer plots of the NMS analysis, Rhododendron schlippenbachii and Rhododendron mucronulatum consistently were affected in shrub layer. In consequence of MRPP-test for changes in vegetation composition, It was analyzed that there are no significant differences for vegetation composition changes on shrub layer in 5-10 years. As a changes of vegetation composition on herb layer were analyzed significantly, composition change of herb layer species was larger than shrub layer species in understory vegetation.

• Journal of the Korean Wood Science and Technology
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• v.41 no.6
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• pp.551-558
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• 2013

Ethanol extracts from Rhododendrom brachycarpum and Abies koreana were investigated for their anti-allergic activities using RBL-2H3 cell line. After treatment with ethanol extracts of various concentrations on the immune response induced mast cell by concanavalin A (Con A), the expressions of cytokine interleukin-4 (IL-4), interleukin-13 (IL-13) were determined by using RT-PCR and the degranulation of mast cells was determined by measuring ${\beta}$-hexosaminidase release. Expression level of IL-4 was decreased by the extract from Rhododendrom brachycarpum in $10^{-7}$, $10^{-5}$ and $10^{-3}%$ concentrations. Expression level of IL-13 was also decreased by both extracts. ${\beta}$-Hexosaminidase release by RBL-2H3 cells was inhibited at the $10^{-5}$ and $10^{-3}%$ concentration of extracts from Rhododendrom brachycarpum and Abies koreana, respectively. These results demonstrate that ethanol extracts of Rhododendron brachycarpum and Abies koreana exert anti-allergic effects by regulating the reduction of IL-4 and IL-13 genes expression and also the secretion of ${\beta}$-hexosaminidase.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.1
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• pp.75-87
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• 2018

This study was carried out to investigate the effects of planting time on the survival rate and growth. Ilex cornuta Lindl., I. aquifolium 'Silver Queen', Ardisia pusilla 'Variegata', and Rhododendron indicum(Satsuki azalea) were planted in pottery pot six times such as April 1, June 1, July 1, August 1, October 1, and December 1 in 2017. The survival rate of I. cornuta Lindl. planted in April, October, and December was 100%, whereas the survival rates were 90% in June, 50% in July, and 60% in August, respectively. The survival rates of I. aquifolium 'Silver Queen' and R. schlippenbachii were also shown about 60% in July and 70% in August, which were lower than in the other planting times at 100%. However, the survival rate of A. pusilla 'Variegata' was shown 100% in the all planting times. The growth rates of plant length, plant height or leaf length or any others of I. cornuta Lindl. were shown 20.0%, 15.5% and 16.5% planted in June, July, and August, respectively, while those planted in April, October and December were 3.2%, 12.3% and 10.7% respectively. Similarly, the growth rates of leaf numbers and plant length of I. cornuta Lindl., A. pusilla 'Variegata', and R. indicum(Satsuki azalea) planted in summer season from June to August for all plat (not only leaf numbers and plant length in was facilitated, while the growth was restrained in planting for spring or autumn. Therefore, the plantation during summer is better for increasing the survival rate and promoting the growth.

• The Korean Journal of Ecology
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• v.14 no.3
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• pp.243-255
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• 1991

The vegetation of haenam peninsula(126 30e.l. and 34 30n.l ) was investigated from July, 1990 to 1991. the units of vegetatin were classified by the phytosociological method of broun- blanquet's. the forest vegetation was classified into 7 communities, sasa borealis-quercus acuta, cinnamomum japonicum, camellia japonica, rhododendron mucronulatum-pinus thunbergii, pinus densiflora community, torreya nucifera, chamaecyparis obtusa afforestatio, and the actual vegetation map was drawn in 1 : 50,00 scaie.