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http://dx.doi.org/10.5658/WOOD.2013.41.6.551

Inhibitory Effect of Extracts from Rhododendron Brachycarpum and Abies Koreana E.H. Wilson on Degranulation and Cytokine Expression in RBL-2H3 Cells  

Jung, Eui-Man (College of Veterinary Medicine, Chungbuk National University)
Kim, Jae-Woo (Department of Forest Products, Korea Forest Research Institute)
Park, Mi-Jin (Department of Forest Products, Korea Forest Research Institute)
Lee, Sung-Suk (Department of Forest Products, Korea Forest Research Institute)
Choi, Don-Ha (Department of Forest Products, Korea Forest Research Institute)
Lee, Jungbok (Sonimedi co., Ltd.)
Jeung, Eui-Bae (College of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of the Korean Wood Science and Technology / v.41, no.6, 2013 , pp. 551-558 More about this Journal
Abstract
Ethanol extracts from Rhododendrom brachycarpum and Abies koreana were investigated for their anti-allergic activities using RBL-2H3 cell line. After treatment with ethanol extracts of various concentrations on the immune response induced mast cell by concanavalin A (Con A), the expressions of cytokine interleukin-4 (IL-4), interleukin-13 (IL-13) were determined by using RT-PCR and the degranulation of mast cells was determined by measuring ${\beta}$-hexosaminidase release. Expression level of IL-4 was decreased by the extract from Rhododendrom brachycarpum in $10^{-7}$, $10^{-5}$ and $10^{-3}%$ concentrations. Expression level of IL-13 was also decreased by both extracts. ${\beta}$-Hexosaminidase release by RBL-2H3 cells was inhibited at the $10^{-5}$ and $10^{-3}%$ concentration of extracts from Rhododendrom brachycarpum and Abies koreana, respectively. These results demonstrate that ethanol extracts of Rhododendron brachycarpum and Abies koreana exert anti-allergic effects by regulating the reduction of IL-4 and IL-13 genes expression and also the secretion of ${\beta}$-hexosaminidase.
Keywords
Rhododendron brachycarpum; Abies koreana E.H. Wilson; IL-4; IL-13; ${\beta}$-hexosaminidase; extractives; anti-allergic activity;
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Times Cited By KSCI : 10  (Citation Analysis)
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