• Journal of Nutrition and Health
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• 제38권8호
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• pp.656-662
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• 2005

Ginger (Zingiber of oficinale Roscoe, Zingiberaceae) is one of the most frequently and heavily consumed dietary condiments throughout the world. Besides its extensive use as a spice, the rhizome of ginger has also been used in traditional oriental herbal medicine for the management of symptoms such as common cold, digestive disorders, rheumatism, neurologia, colic, and motion-sickness. The oleoresin from rhizomes of ginger contains [6] -gingerol (1- [4'-hydroxy-3'-methoxyphenyl]-5-hydroxy-3-decanone) and its homologs as pungent ingredients that have been found to possess many interesting pharmacological and physiological activities, such as anti-inflammatory, analgesic, antipyretic, antiheatotoxic, and cardiotonic effects. However, the effect of [6]-gingerol on cell proliferation in breast cancer cell are not currently well known. Therefore, in this study, we examined effect of [6]-gingerol on protein and mRNA expression associated with cell proliferation in MDA-MB-231 human breast. cancer cell lines. We cultured MDA-MB-231 cells in presence of 0, 2.5, 5 and $10{\mu}M$ of [6] -gingerol. [6]-Gingerol inhibited breast cancer cell growth in a dose-depenent manner as determined by MTT assay. ErbB2 and ErbB3 protein and mRNA expression were decreased dose-dependently in cells treated with [6]-gingerol (p<0.05). In addition, phosphorylated Akt levels and total hぉ levels were markedly decreased in cells treated with $2.5{\mu}M$ [6]-gingerol (p<0.05). In conclusion, we have shown that [6]-gingerol inhibits cell proliferation through ErbB2 and ErbB3, reduction in MDA-MB-231 human breast cancer cell lines.

• 한국식품영양과학회지
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• 제12권4호
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• pp.357-363
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• 1983

인삼(人蔘)의 근부(根部)(뇌두(腦頭), 표피(表皮), 동체피층(胴體皮層), 동체내층(胴體內層), 지근(支根), 세미(細尾))와 지상부(地上部)(과육(果肉), 종자(種字), 경(莖), 엽(葉))의 부위별(部位別) 지방질의 함량과 그 구성지방산을 조사하였다. 9부위(部位)의 유리지질(遊離脂質)과 결합지질(結合脂質)을 합한 총 지방질 함량은 0.91~3.48%였으나 종자(種子)는 15.08%로 다른 부위(部位)에 비하여 그 함량이 대단히 높았다. gas liquid chromatography에 의하여 14종(種)의 짝수지방산과 4종(種)의 홀수지방산을 분리, 정량하였으며 주된 지방산은 linoleic acid, palmitic acid, oleic acid 및 linolenic acid였다. 부위별(部位別) 지방산 조성은 현저한 차이를 나타내고 있었으며 특히 종자(種子)는 부위(部位)들과는 상당한 차이가 있었으며 10종(種)의 지방산만이 동정되었고 oleic acid와 linoleic acid(51.21%와 37.46%)가 다른 부위(部位)들보다 상당히 많았다. 또한 종자(種子), 동체내층(胴體內層) 및 동체피층(胴體皮層)에 함유된 유리지질(遊離脂質)은 다른 부위(部位)의 지방질(脂肪質)에 비하여 불포화 지방산의 함량이 높았다.

• Journal of the Korean Wood Science and Technology
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• 제46권3호
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• pp.260-269
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• 2018

경산 임당 유적에서 출토된 2-4세기로 추정되는 목제유물의 표면에 존재하는 도구흔적을 분석하여 목제유물의 제작도구와 제작방법을 해석하고자 하였다. 97점의 목제유물의 표면에 존재하는 도구흔적을 분석한 결과, 이들 유물의 제작에 자귀, 끌, 송곳, 손칼, 도끼 등 다양한 도구가 이용되었음이 확인되었다. 특히 갈이틀과 갈이칼의 흔적이 확인됨으로써, 이 시기에 선삭가공 방법이 사용되었음을 알 수 있었다. 또한 선삭가공으로 용기류 목제유물 제작 시 눈질과 선질을 혼합하여 사용했음이 확인되었다. 이는 제작하고자 하는 용기류 목제유물의 형태 및 용도에 따라 적합한 가공방법을 적용한 것으로 판단된다.

• Archives of Pharmacal Research
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• 제22권2호
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• pp.219-224
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• 1999

In our search for the anticonvulsant consitutent of Gastrodia elata repeated column chromatographies guided by activity assay led to isolation of an active compound, which was identified as gastrodin on the basis of spectral data. Brain succinic semialdehyde dehydrogenase (SSADH) was inactivated by preincubation with gastrodin in a time-dependent manner and the reaction was monitored by absorption and fluorescene spectroscopic methods. The inactivation followed pseudo-first-order kinetics with the second-rate order constant of $1.2{\times}10^{3} M^{-1} min^{-1}$. The time course of the reaction was significantly affected by the coenzyme NAD^{+}$, which affected complete protection against the loss of the catalytic activity, whereas substrate succinic semialdehyde failed to prevent the inactivation of the enzyme. It is postulated that the gastrodin is able to elevate the neurotransmitter GABA levels in central nervous system by inhibitory action on one of the GABA degradative enzymes, SSADH.

• 식물분류학회지
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• 제34권4호
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• pp.297-319
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• 2004

한국산 사초속 사초아속 (Carex subgen. Carex)의 염주사초절 (sect. Ischnostachyae), 참삿갓 사초절 (sect. Anomalae), 바늘사초절 (sect. Capitellatae), 길뚝사초절 (sect. Debiles), 묏풀사초절 (sect. Capillares), 애기흰사초절 (sect. Molliculae) 등 총 6절 14분류군을 대상으로 외부형태학적 형질을 재검토하고, 광학현미경과 주사전자현미경을 사용하여 과낭, 수과, 잎의 표피형을 비교 분석하였다. 그 결과 얻어진 정량적 형질(줄기, 잎, 이삭, 비늘조각, 포, 과낭, 수과의 길이와 너비, 잎의 기공복합체의 크기와 빈도, 기본세포줄, 기공줄의 수)과 정성적 형질[암/수꽃이삭, 비늘조각, 과낭, 수과, 과낭, 수과의 표피세포, 잎의 표피 구성요소(기본표피세포의 모양, 세포벽의 굴곡, 규소체의 모양, 부세포의 모양), 잎의 앞 뒷면의 유두돌기, 가시돌기 및 털의 유무] 등이 종을 동정하고 식별하는데 유용하였다. 본 연구 결과 이삭의 수, 암/수꽃이삭의 배열, 포의 유무로 바늘사초절과 나머지절로 2개의 그룹으로 크게 나눌 수 있었다.

• 동의생리병리학회지
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• 제33권2호
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• pp.116-122
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• 2019

The aim of this study is to evaluate the anti-inflammatory effect of Hataedock treatment using Coptidis Rhizome and Glycyrrhiza Uralensis (CG) mixed extract in allergic rhinitis induced NC/Nga mice. We divided NC/Nga mice into 3 groups as follows; allergic rhinitis-induced group after CG Hataedock treatment (CGT, n=10), no treatment group (Ctrl), allergic rhinitis elicited group (ARE). To induce allergic rhinitis, NC/Nga mice of 3 weeks age were sensitized on 7, 8 and 9week by Ovalbumin (OVA) antigen in intranasal space. Hataedock using CG extract was administered on week 3 in allergic rhinitis-induced group (CGT) after Hataedock treatment. To identify distribution of Interlukin (IL)-4, Cluster of differentiation 40 (CD40), high-affinity IgE receptor ($Fc{\varepsilon}RI$), substance P, Matrix metallopeptidase 9 (MMP-9), Nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) p65, Inducible nitric oxide synthase (iNOS) and Cycloxygenase-2 (COX-2), we used histological examination. CGT significantly inhibited IL-4 and CD40 response compared with ARE. The reduction of Th2 cytokine expression decreased inflammatory mediators such as $Fc{\varepsilon}RI$, substance P, MMP-9, $NF-{\kappa}B$ p65, iNOS and COX-2. Such immunological improvement induced reduction of respiratory epithelial damage and mucin secretion in goblet cell. These results indicate that Hataedock treatment suppresses allergic rhinitis through modulating of Th2 responses and diminishing various inflammatory mediators in nasal mucosal tissue. It might have potential applications for prevention and treatment of allergic rhinitis.

• 대한한의학방제학회지
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• 제27권3호
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• pp.189-197
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• 2019

Objectives : The purpose of this study was to confirm whether Codonopsis Radix(CR) could be used in the same way for expected indications or diseases of adaptation instead of Ginseng Radix(GR), which acts as a principal herb in Sagunja-tang. Methods : The Traditional Chinese Medicine Systems pharmacology(TCMSP), a database for the study of systems biology related to Chinese medicine, screened potential active compounds in each quartet. By searching for all the proteins that each compound provides, the target of Sagunja-tang with GR(GRST) and the target of Sagunja-tang with CR(CRST) were compared using the network analysis method, and the top ranked target of each serving was selected. Results : Through TCMSP, a Chinese medicine database, the potential effective ingredients of GRST or CRST screened, and the target proteins related to these substances were found to be the most affected by Glycyrrhizae Radix et Rhizome, an herbal medicine mixed in Sagunja-tang, and the target diseases were the same. And the same were found for the target protein, gene and target diseases of GRST and CRST. Conclusions : The prescription with similar composition is likely to have similar network pharmacology analysis results, and the analysis result may be controlled by the herbal medicines which are assumed to be the main function. Therefore, rich and reproducible basic studies is more important because network pharmacological studies can be dominated by data that has been done a lot of previous studies.

• 한국산업융합학회 논문집
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• 제22권1호
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• pp.61-69
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• 2019

All the parts of lotus, including the seed, rhizome, leaf, stalk, petal, anther, pericarp, and fruit receptacle, have been used in traditional medicine system as a health beneficial supplement. However the most usually used from lotus plant is only the root. Therefore in this study, it will be discussed more the utilization of other parts of the lotus, namely the flower of lotus. The petals and stamens of lotus actually are also rich in bioactive components such as flavonoids and alkaloids, are used in the treatment of tissue inflammation, cancer, skin disease, and also for us as antidotes. One of the biotechnological process that can be used to improve the nutritional content, sensory, and also antioxidant activities is fermentation process. The final product desired from the fermentation process in this study is vinegar. The microbial strain powder used is Uinkin fermented powder with three variations of fermentation. The variations given in this study were initial sugar 32%, 24%, and 14% with the same fermentation temperature, $35^{\circ}C$ for 3 months. The results obtained showed that the pH value and sugar content of products during the fermentation process were decreasing during the fermentation process, with total polyphenol content of $283.7{\pm}97.6mg/100g\;QAE$, and total flavonoid content of $3.3{\pm}0.0mg/100g\;QAE$. For the DPPH radical scavenging ability of the fermentation product also increased in a concentration dependent manner, with ORAC activity of the product showed a high activity of $20.7{\pm}0.41{\mu}M$ TE. Therefore, fermentation process can be the one of method for improving the product. The efficiency of lotus flower vinegar fermentation can be reached with an initial sugar condition of 25% (sample B).

• 한국전자통신학회논문지
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• 제16권3호
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• pp.493-502
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• 2021

외부 환경변화에 적응하여 MPP를 추적할 수 있는 포복경 영양 번식(VPR; Vegetative Propagation by Runner) 최적화 알고리즘 기반 MPPT 알고리즘을 제시하였다. VPR 알고리즘은 영양기관을 기반으로 군집 이동 번식하는 식물 생태를 모방한 알고리즘으로 식물의 노화 및 부근(Rhizome)에 대한 주변탐색 절차를 수행하여 최적점 인근의 주변을 지속적으로 탐색할 수 있다. 따라서 VPR 기반 MPPT 알고리즘의 경우, MPPT 알고리즘이 수행되는 시점에 발생하는 외부 환경변화에 적응하여 최적점을 탐색할 수 있다. 본 논문에서는 다수의 모의실험을 통해 VPR 기반 MPPT 알고리즘의 성능을 분석하였다. 더불어 PSO(Particle Swarm Optimization) 기반 MPPT 알고리즘과 동일한 환경에서 성능 비교를 통해 성능의 우수성을 비교하였다.

• 한국유기농업학회지
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• 제29권4호
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• pp.575-588
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• 2021

본 연구에서는 오이 지상부에 발생하는 두 가지 병원균 B. cinerea와 C. orbiculare에 항균활성을 가지는 유용한 방선균을 선발하고자 하였다. 토양에서 분리한 방선균 560 균주 중 두 병원균에 대해 동시 항균활성을 나타낸 7균주를 일차적으로 선발한 후, 배양여액의 항균활성 검정을 위한 대치배양과 오이 식물체 검정을 통해 최종적으로 S20-465 균주를 선발하였다. 이 균주는 염기서열 비교 분석을 통해 Streptomyces sp.로 동정하였다. S20-465 균주의 배양여액은 두 병원균의 균사 생장을 60%이상 억제하였다. 배양여액의 20배, 40배 희석액을 분무 처리할 경우 오이 잎에서의 잿빛곰팡이병에 의한 병반길이를 감소시켰을 뿐만 아니라 탄저병의 발병도도 대조구에 비해 50% 이상 감소시켜 생물검정에서도 병억제 효과가 있는 것을 확인하였다. 따라서 최종 선발한 S20-465는 앞으로 생물방제 미생물소재로써 활용할 수 있을 것으로 사료된다.