• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.143.2-143
• /
• 2003

A rapid and sensitive assay for the specific detection of plant viroids using reverse transcription-polymerase chain reaction(RT-PCR) has been developed already. The nested RT-PCR assay cloud be applied for the detection of apple scar skin viroid(ASSVd) from young leaves and other tissues. ASSVd has central conserved region(CCR), terminal left(T$\sub$L/) and terminal right(T$\sub$R/) domain. Primers were designed from these regions. Primer sets were successfully applicable for the amplification of full length or partial region of ASSVd by nested RT-PCR. Nested RT-PCR assay was more sensitive and accurate method to detect ASSVd from young trees during the early time of apple cultivation.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.150.1-150
• /
• 2003

A single-step multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for the simultaneous detection of five cucurbit-infecting viruses： cucumber mosaic virus (CMV), watermelon mosaic virus 2 (WMV2), zucchini yellow mosaic virus (ZYMV), cucumber green mottle mosaic virus (CGMMV), and kyuri green mottle mosaic virus (KGMMV). The multiplex RT-PCR provides a simple and rapid method for detecting various viruses in cucurbit plants, which will help diagnose many cucurbit plants at a time.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.7 no.4
• /
• pp.212-215
• /
• 2002

Tyrosinase transcript In the blood Is known as the marker of malignant melanoma and it has been often determined by using reverse transcription-polymerase chain reaction (RT-PCA) . However, after the PCR process, the quantification of amplified CDMA by the gel electrophoresis is not reliable and time-consuming. for this reason, we tried to quantify the PCR product using a cuvette-type biosensor, where the oligonucleotide probe was immobilized on the cuvette surface and the single strand CDMA, the denatured PCH product, was then hybridized onto the immobilized probe to give a response signal. The response was Immediate and takes 15 min to obtain a stable signal. The biosensor was much more sensitive comparing to the gel electrophoresis method. The quantification of PCR product using a cuvette-type biosensor was feasible and rapid.

• Proceedings of the Materials Research Society of Korea Conference
• /
• 2003.03a
• /
• pp.118-118
• /
• 2003

전력반도체 소자로 사용되는 p$^{-}$-n$^{-}$ 접합 다이오드의 스위칭 속도를 향상시키고 그에 따른 에너지 손실을 감소시키기 위해 전자 조사를 실시하였다. Reverse recovery time이 현저히 감소한 반면, 전자 조사에 의한 누설전류와 on-state 전압 강하와 같은 그 외의 전기적 특성 저하는 무시할 수 있는 정도였다. 그밖에 시료의 deep level transient spectroscpy(DLTS) 분석 결과와 secondary ion mass spectrometry(SIMS) depth profile을 근거로 결함 분포와 전자조사 유도결함의 유형을 논하였다.

• International Journal of Precision Engineering and Manufacturing
• /
• v.3 no.4
• /
• pp.15-23
• /
• 2002

With the development of a laser scanner of high precision and increased speed, reverse engineering becomes a key approach to reduce the time for the development of new products. But the modeling process is not so automated enough until now. Modeling in real workshops is usually performed by the experienced operators and it requires a skillful technique to get the resultant surface of high quality and precision. In this paper, a systematic solution is proposed to automate the free-form surface generation from the measured point data. Compatibility is imposed to the measured point data during input curve generation. And the compatibility of cross-sectional curve is also considered for the loft surface generation. The data in each step is produced in IGES file format to make an easy interface to other CAD/CAM software without any further data manipulation.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.63 no.2
• /
• pp.291-296
• /
• 2014

Most of the statistical signal analysis processed in the time domain and the frequency domain are based on the assumption that the signal is weakly stationary(wide sense stationary). Therefore, it is necessary to know whether the surface EMG signals processed in the statistical basis satisfy the condition of weak stationarity. The purpose of this study is to analyze the accuracy of the Run-test, modified Run-test, RA(reverse arrangement)-test, and modified RA-test for assessing surface EMG signal stationarity. Six stationary and three non-stationary signals were simulated by using sine wave, AR(autoregressive) modeling, and real surface EMG. The simulated signals were tested for stationarity using nine different methods of Run-test and RA-test. The results showed that the modified Run-test method2 (mRT2) classified exactly the surface EMG signals by stationarity with 100% accuracy. This finding indicates that the mRT2 may be the best way for assessing stationarity in surface EMG signals.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2001.07a
• /
• pp.33-36
• /
• 2001

Chemical mechanical polishing(CMP) process has been widely used to planarize dielectric layers, which can be applied to the integrated circuits for deep sub-micron technology. The rise throughput and the stability in the device fabrication can be obtained by applying of CMP process to STI structure in 0.18$\mu\textrm{m}$ m semiconductor device. The reverse moat process has been added to employ in of each thin films in STI-CMP was not equal, hence the devices must to be effected, that is, the damage was occurred in the device area for the case of excessive CMP process and the nitride film was remained on the device area for the case of insufficient CMP process, and than, these defects affect the device characteristics. Also, we studied the High Selectivity Slurry(HSS) to perform global planarization without reverse moat step.

• Korean Journal of Materials Research
• /
• v.9 no.4
• /
• pp.419-427
• /
• 1999

TiNi shape memory alloy was shape memory heat-treated and investigated its mechanical properties with the variation of prestrain. Also 6061 Al matrix composites with TiNi shape memory alloy fiber as reinforcement have been fabricated by Permanent Mold Casting to investigate the microstructures and interface properties. Yield stress of TiNi wire was the most high in the case of before heat-treatment and then decreased as increasing heat-treatment time. In each heat-treatment condition, the yield stress of TiNi wire was not changed with increasing the amount of prestrain. The interface bonding of TiNi/6061Al composite was fine. There was a 2$\mu\textrm{m}$ thickness of diffusion reaction layer at the interface. We could find out that this diffusion reaction layer was made by the mutual diffusion. The diffusion rate from Al base to TiNi wire was faster than that of reverse diffusion and the amount of the diffusion was also a little more than that of reverse.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2001.04a
• /
• pp.895-898
• /
• 2001

This paper presents an optimization cutting speed(OCS) program developed to improve the machining precision and tool life in high speed machining using ball end milling. This program optimized the cutting speed that is changing at any time in free surface machining of an automobile part like a connecting load die. The technique of optimization cutting speed makes the CAD/CAM-generated NC code go through a reverse post process, conducts cutting simulation, and obtain the effective tool diameter of the ball end milling. Then it changes the spindle revolution to within the range of critical cutting speed fit for the material of the workpieces depending upon the effective tool diameter. In this study, the machining precision and tool life were compared for the two connecting load dies processed using the general cutting method and the proposed optimization cutting speed technique, respectively.

• Journal of Advanced Marine Engineering and Technology
• /
• v.30 no.7
• /
• pp.800-807
• /
• 2006

This paper introduces the hull deflection analysis method by using the direct measurements. Accordingly, this paper demonstrates how the hull deflection data is obtained by the reverse calculations using the bending moments from the stain gauge and bearing reactions from jack-up method. Where the hull deflection data provided by this research is used for the shafting alignment calculations for identical or similar vessels, shafting failures due to hull deflections can be minimized. It will also save time and expenses associated finite element method to predict hull deflections.