Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)

The Korean Society of Plant Pathology (한국식물병리학회)
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Sensitive method for the detection of Apple scar skin viroid(ASSVd) by nested reverse transcription-polymerase chain reaction

  • Lee, Sung-Joon (Department of Microbiology, Kyungpook National University) ;
  • Kim, Chung (Department of Microbiology, Kyungpook National University) ;
  • Sim, Sang-Mi (Department of Microbiology, Kyungpook National University) ;
  • Lee, Dong-Hyuk (Daegu Apple Research Institute, National Horticultural Research Institute) ;
  • Lee, Jai-Youl (Department of Microbiology, Kyungpook National University)
  • Published : 2003.10.01
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Abstract

A rapid and sensitive assay for the specific detection of plant viroids using reverse transcription-polymerase chain reaction(RT-PCR) has been developed already. The nested RT-PCR assay cloud be applied for the detection of apple scar skin viroid(ASSVd) from young leaves and other tissues. ASSVd has central conserved region(CCR), terminal left(T$\sub$L/) and terminal right(T$\sub$R/) domain. Primers were designed from these regions. Primer sets were successfully applicable for the amplification of full length or partial region of ASSVd by nested RT-PCR. Nested RT-PCR assay was more sensitive and accurate method to detect ASSVd from young trees during the early time of apple cultivation.

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