• Preventive Nutrition and Food Science
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• 제22권2호
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• pp.81-89
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• 2017

Healthy aging has become a major goal of public health. Many studies have provided evidence and theories to explain molecular mechanisms of the aging process. Recent studies suggest that epigenetic mechanisms are responsible for life span and the progression of aging. Epigenetics is a fascinating field of molecular biology, which studies heritable modifications of DNA and histones that regulate gene expression without altering the DNA sequence. DNA methylation is a major epigenetic mark that shows progressive changes during aging. Recent studies have investigated aging-related DNA methylation as a biomarker that predicts cellular age. Interestingly, growing evidence proposes that nutrients play a crucial role in the regulation of epigenetic modifiers. Because various nutrients and their metabolites function as substrates or cofactors for epigenetic modifiers, nutrition can modulate or reverse epigenetic marks in the genome as well as expression patterns. Here, we will review the results on aging-associated epigenetic modifications and the possible mechanisms by which nutrition, including nutrient availability and bioactive compounds, regulate epigenetic changes and affect aging physiology.

• BMB Reports
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• 제42권5호
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• pp.271-276
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• 2009

Sweetpotato (Ipomoea batatas (L). Lam.) is relatively tolerant to unfavorable growth conditions such as drought, yet has not been exploited to provide a better understanding of the molecular basis of drought stress tolerance. We obtained 983 high-quality expressed sequence tags of 100 bp or longer (average length of 700 bp) from cDNA libraries of detached white fibrous root tissues by subjecting them to dehydration for 6 h. The 431 cDNAs were each assigned a function by alignment using the BLASTX algorithm. Among them, three genes associated with various abiotic stresses and nine genes not previously associated with drought stress were selected for expression pattern analysis through detailed reverse transcription-polymerase chain reaction. The direct and indirect relationships of the 12 genes with drought tolerance mechanisms were ascertained at different developmental stages and under various stress conditions.

• Wind and Structures
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• 제29권6호
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• pp.441-455
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• 2019

In this study, vortex induced vibrations of a cylinder mounted on a flexible rod are analyzed. This simple configuration represents the key element of new conception bladeless wind turbine (Whitlock 2015). In this study the structure oscillations equation coupled to the wake oscillation equation for this configuration are solved using analytical perturbation method, for the first time. An analytical expression that predicts the lock-in phenomena range of wind speed is derived. The discretized equations of motion are also solved using RKF45 numerical method. The equations of motion are discretized by Galerkin method. Free vibration mode shape of the structure taking into account the discontinuity of the cross section are used as comparison function. Numerical results are compared to the analytical results, and they show a satisfying agreement. The effect of system parameters on the oscillations of structure and wake as well as on the lock-in domain are presented. Moreover, it is shown that the values of wind speed triggering the start and the stop of the lock-in phenomenon, for increasing wind speed are different from those values obtained during the reverse process, i.e., when the wind speed decreases.

• BMB Reports
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• 제44권12호
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• pp.805-810
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• 2011

Methionine sulfoxide reductase A (MSRA) is a ubiquitous enzyme that has been demonstrated to reduce the S enantiomer of methionine sulfoxide (MetSO) to methionine (Met) and can protect cells against oxidative damage. In this study, we isolated a novel MSRA (SlMSRA2) from Micro-Tom (Solanum lycopersicum L. cv. Micro-Tom) and characterized it by subcloning the coding sequence into a pET expression system. Purified recombinant protein was assayed by HPLC after expression and refolding. This analysis revealed the absolute specificity for methionine-S-sulfoxide and the enzyme was able to convert both free and protein-bound MetSO to Met in the presence of DTT. In addition, the optimal pH, appropriate temperature, and $K_m$ and $K_{cat}$ values for MSRA2 were observed as 8.5, $25^{\circ}C$, $352{\pm}25\;{\mu}M$, and $0.066{\pm}0.009\;S^{-1}$, respectively. Disk inhibition and growth rate assays indicated that SlMSRA2 may play an essential function in protecting E. coli against oxidative damage.

• The Journal of Asian Finance, Economics and Business
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• 제1권3호
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• pp.5-16
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• 2014

This study aims to examine the dynamics of price changes and trading volume of Kuala Lumpur Options and Financial Futures Exchange (KLOFFE) from 2000 to 2008. With augmented analysis, our results support two hypotheses. First, under information spillover, our findings support noise traders' hypothesis as the time span for variance of past trading volume to cause variance of current return is found to be asymmetric under bull and bear markets. Second, looking at the dynamic relation between volume and volatility of price changes, our findings support Liquidity-Driven Trade hypothesis as past trading volume and subsequent volatility of return exhibit positive correlation. In terms of investors' behavior in response to the news, we find that investors are more risk taking in bull market and more risk reverse in bear market. Our study suggests that investors should adjust their strategy in the futures market in a dynamic manner as the time span of new information arrival is not consistent. Also, uninformed investors with information asymmetry should expect noninformational trading from informed investors to establish their desired positions for better liquid position.

• Fisheries and Aquatic Sciences
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• 제18권2호
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• pp.173-181
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• 2015

We identified the $Na^+-K^+-2Cl^-$ cotransporter 2 (NKCC2) cDNA isoform from starry flounder, Platichthys stellate. The NKCC2 cDNA encoded a polypeptide of 1,043 amino acids representing 12 putative transmembrane domains based on the bioinformatic topology prediction. In addition, starry flounder NKCC2 possessed highly conserved residues within transmembrane domain 4, known as an essential site for its function. End-point reverse transcription-polymerase chain reaction analysis revealed that the NKCC2 transcript was moderately expressed only in the anterior and posterior intestines and the rectum. The NKCC2 mRNA level in the rectum, but not in other segments, was significantly induced 3 days post Streptococcus parauberis challenge, indicating that excess salt may be transported into the rectum. Taken together, our data indicate that an S. parauberis infection could tip the intestinal fluid balance in favor of fluid accumulation, indicating that bacterial pathogens can interfere with intestinal osmotic balance and normal mucosal immune homeostasis.

• 한국연소학회지
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• 제3권2호
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• pp.13-27
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• 1998

The optimization of frontal device including fuel nozzle and swirler is required to secure the mixing of fuel and air and the combustion stability leading the reduction of pollutant emissions and the increase of combustion efficiency in gas turbine combustor. The effects of injection nozzle and swirler on the flow field, spray characteristics and consequently the combustion stability, were experimentally investigated by measuring the velocity field, droplet sizes of fuel spray, lean combustion limit and the temperature field in the main combustion region. Flow fields and spray characteristics were measured with APV(Adaptive Phase Doppler Velocimetry) under atmospheric condition using kerosine fuel. Temperatures were measured by Pt-Pt13%Rh, R-type thermocouple which was 0.2mm thick. Spray and flame was visualized by ICCD(Intensified Charge Coupled Device) camera. It was found that the dual swirler resulted in the biggest recirculation zone with the highest reverse flow velocity at the central region, which lead the most stable combustion. The various combustion characteristics were observed as a function of the geometries of injector and swirler, that gave a tip for the better design of gas turbine combustor.

• 대한기계학회논문집A
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• 제32권11호
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• pp.970-977
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• 2008

This paper deals with the dynamic characteristics of the shaft with impeller model which is the most important part in developing the resin mixing machine. Through reverse engineering, it is possible to make the shaft with impeller geometry model which is necessary vibration characteristic analysis by commercial impeller. The natural frequency analysis and structural analysis using finite element analysis software are performed on the imported commercial shaft with impeller model. The most important fundamental natural frequency of the shaft with impeller model is around 14.5 Hz, which well agrees with modal testing. The most effective design variables were extracted by ANOM(analysis of means) and pareto chart. This paper presents approximation 2nd order polynomial as design variables using RSM(response surface methodology). Generally, RSM take 2 or 3 design variables, but this method uses 5 design variables with table of mixed orthogonal array. Further more, the analyzed result of the commercial shaft with impeller is to be utilized for the structural design of resin chock mixing machine.

• Molecules and Cells
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• 제40권10호
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• pp.796-804
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• 2017

Endogenous retroviruses (ERVs) have been integrated into vertebrate genomes and have momentously affected host organisms. Horses (Equus caballus) have been domesticated and selected for elite racing ability over centuries. ERVs played an important role in the evolutionary diversification of the horse genome. In the present study, we identified six equine ERV families (EqERVs-E1, I1, M2, P1, S1, and Y4), their full-length viral open reading frames (ORFs), and elucidated their phylogenetic relationships. The divergence time of EqERV families assuming an evolutionary rate of 0.2%/Myr indicated that EqERV-S3 (75.4 million years ago; mya) on chromosome 10 is an old EqERV family and EqERV-P5 (1.2 Mya) on chromosome 12 is a young member. During the evolutionary diversification of horses, the EqERV-I family diverged 1.7 Mya to 38.7 Mya. Reverse transcription quantitative real-time PCR (RT-qPCR) amplification of EqERV pol genes showed greater expression in the cerebellum of the Jeju horse than the Thoroughbred horse. These results could contribute further dynamic studies for horse genome in relation to EqERV gene function.

• Journal of Photoscience
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• 제1권2호
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• pp.95-105
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• 1994

Experiments were carried out to investigate whether P, deficiency in detached 25 mM mannose-feeding led to a decline of the photosynthetic electron transport rates through acidification of the thylakoid lumen. With increasing mannose-feeding time, the maximal CO2 exchange rates and the maximal quantum yields of photosynthesis decreased rapidly up to 6 h by 73% then with little decrease up to 12 h. The ATP/ADP ratio declined by 54% 6 h after the treatment and then recovered to the control level at 12 h. However, the NADPH/NADP~ ratio was not significantly altered by mannose treatment. Electron transport rates of thylakoid membranes isolated from 6 h treated leaves did not change, but they decreased by 30% in 12 h treated leaves. The quenching analysis of Chl fluorescence in mannose-treated leaves revealed that both the fraction of reduced plastoquinone and the degree of acidification of thylakoid lumen remained higher than those of the control. The reduction of PSI in mannose fed leaves was inhibited due to acidification of thylakoid lumen (high qE). The reduction of primary quinone acceptor of PSII was inhibited by mannose feeding. Mannose treatment decreased the efficiency of excitation energy capture by PSII. Fo quenching was induced when treated with mannose more than 6 h, and had a reverse linear correlation with (Fv)m/Fm ratio. These results suggest that Pi deficiency in Chinese cabbage leaves reduce photosynthetic electron transport rates by diminishing both PSII function and electron transfer from PSII to PSI through acidification ofthylakoid lumen, which in turn induce the modification of photosynthetic apparatus probably through protein (de)phosphorylation.