• Journal of Nutrition and Health
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• 제35권10호
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• pp.1053-1059
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• 2002

In post-genome period, the technique for identifying gene expression has been changed to high throughput screening. In the field of molecular nutrition, the need for this technique to clarify molecular function of the specific nutrient is essential. In this study, we have tested the zinc-regulated gene expression in zinc-deficient U937 cells, using cDNA microarray which is the cutting-edge technique to screen large numbers of gene expression simultaneously. The study result can be used for the preliminary gene screening data for clarifying, using monocyte U937 cell line, molecular Zn aspect in atherosclerosis. U937 cells were cultured in Zn-adequate (control, 12 $\mu$M Zn) or Zn-deficient (experimental, 0 $\mu$M Zn) ESMI media during 2 days, respectively. Cells were harvested and RNA was extracted. Total RNA was reverse-transcriptinized and synthesized cDNA probe labeled with Cy-3. fluorescent labeled cDNA probe was applied to microarray slide for hybridization slide, and after then, the slide was scanned using fluorescence scanner. ‘Highly expressed genes’ in Zn-deficient U937 cells, comparing to Zn-adequate group, are mainly about the genes for motility protein, immune system protein, oncogene and tumor suppressor and ‘Less highly expressed genes’ are about the genes for transcription, apoptosis associated protein, cell cycle, and several basic transcription factors. The results of this preliminary study imply the effectiveness of cDNA microarray for expression profiling of a singly nutrient deficiency, specially Zn. Furthur study, using tailored-cDNA array and capillary endothelial cell lines, would be beneficial to clarify molecular Zn function, more in detail.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.350-356
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• 2019

Objective: To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells. Results: IL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide ($H_2O_2$)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue. Conclusion: We confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.

• 대한본초학회지
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• 제36권5호
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• pp.59-67
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• 2021

Objectives : Hibiscus (Hibiscus sabdariffa L.) is rich in antioxidants such as flavonoids and anthocyanins and is known to have anti-inflammatory activity and anti-aging function of the skin, but there is no study on its effect on the skin barrier. This study aim to investigate the positive effect on the skin barrier by confirming the effect of water extracts of Hibiscus sabdariffa L. (WEHS) on the tight junction-related gene expression. Methods : The antioxidant efficacy of WEHS was investigated through ABTS and DPPH assays, and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium was performed to examine the effect on cell viability. quantitative Reverse transcription polymerase chain reaction and immunoblot analysis were performed to confirm the effect of WEHS on the expression of tight junction-related genes, and immunofluorescence microscopy was used to confirm the movement of Claudin 1 protein into tight junctions. Results : WEHS showed strong antioxidant activity and induced an increase in both mRNA and protein expression levels of Claudin 1 among tight junction-related genes. The strong localization of Claudine 1 protein increased by WEHS to the tight junction was confirmed by immunofluorescence microscopy. Conclusions : Hibiscus was confirmed through this study to show antioxidant activity and the function of promoting the expression of the tight junction Claudin 1 gene, suggesting that Hibiscus can be used as a material for the prevention and treatment of skin moisturizing and atopy, which have an important influence on tight junction.

• 대한본초학회지
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• 제33권5호
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• pp.1-8
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• 2018

Objectives : The aim of this study is to investigate the preventive effect of Puerariae Flos ethanol extract (PE) on methionin and choline deficient (MCD)-diet-induced Nonalcoholic fatty liver disease (NAFLD) in C57BL/6J mice. Methods : In the in vivo experiments, C57BL/6J mice were divided into 4 groups; Normal group, Control group, MCD+PE 100 group, and MCD+PE 300 group. After 4 weeks, body weight, liver weight, biochemical parameters for liver function test, histological changes, reverse transcription polymerase chain reaction (RT-PCR), and Western blot were assessed. Results : Mice lost body weight with the MCD-diet and the MCD+PE 100 group and MCD+PE 300 groups lost less than the control group, though showed no statistical significance. Liver weights were decreased by the MCD diet, but MCD+PE 300 groups were increased significantly. In the liver function test, all the values were decreased with the MCD-diet, MCD+PE 100 group and MCD+PE 300 groups were increased significance. In histological findings of the livers, MCD-diet induced severe fatty accumulation in the livers, but this fatty change was reduced in the MCD+PE 100 group and MCD+PE 300 groups was inhibited respectively. In lipid accumulation factors (such as SREBP-1c, $C/EBP{\alpha}$, PPAR-${\gamma}$), MCD+PE 100 group and MCD+PE 300 groups showed inhibitory effect on liver lipogenesis by reducing associated gene expressions caused by MCD diet. Conclusions : We were able to know that Puerariae Flos ethanol extract (PE) shown hepatoprotective effects via a decrease on the hepatic lipogenesis factors in the experimental NAFLD Models.

• 한국산업정보학회논문지
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• 제29권3호
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• pp.23-40
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• 2024

하드웨어 공격은 암호화 키 혹은 회로 설계와 같은 민감한 정보를 훔치기 위해 물리적인 역공학 작업을 수반한다. 암호화와 난독화는 대표적인 대응책이지만, 공격자가 키를 알아내면 무력화된다. 이 문제를 해결하기 위해 본 연구에서는 물리적으로 복제할 수 없는 기능 (Physically Unclonable Function)을 위변조 방지 방패로 활용하는 e-Cryptex를 제안한다. PUF는 난수 생성기 역할을 하며 복제나 복원할 수 없는 고유한 물리적 변형을 사용해 변조 방지 메커니즘을 강화한다. e-Cryptex는 시스템 구조를 보호하고 키를 생성하기 위해 PUF를 실드로 사용한다. 실드를 변조하면 키가 파괴된다. 본 논문은 e-Cryptex가 PUF 보안 요구 사항을 충족하며 실드를 뚫거나 완전히 파괴하는 변조 시도를 탐지하는 데 효과적임을 입증한다. 각 보드는 정상적인 조건에서 일관되게 같은 키를 생성하는 동시에 여러 보드에서 키 고유성을 보여준다.

• Korean Circulation Journal
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• 제54권4호
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• pp.172-186
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• 2024

Background and Objectives: Long-term pathological myocardial hypertrophy (MH) seriously affects the normal function of the heart. Dronedarone was reported to attenuate left ventricular hypertrophy of mice. However, the molecular regulatory mechanism of dronedarone in MH is unclear. Methods: Angiotensin II (Ang II) was used to induce cell hypertrophy of H9C2 cells. Transverse aortic constriction (TAC) surgery was performed to establish a rat model of MH. Cell size was evaluated using crystal violet staining and rhodamine phalloidin staining. Reverse transcription quantitative polymerase chain reaction and western blot were performed to detect the mRNA and protein expressions of genes. JASPAR and luciferase activity were conducted to predict and validate interaction between forkhead box O3 (FOXO3) and protein kinase inhibitor alpha (PKIA) promoter. Results: Ang II treatment induced cell hypertrophy and inhibited sirtuin 1 (SIRT1) expression, which were reversed by dronedarone. SIRT1 overexpression or PKIA overexpression enhanced dronedarone-mediated suppression of cell hypertrophy in Ang II-induced H9C2 cells. Mechanistically, SIRT1 elevated FOXO3 expression through SIRT1- mediated deacetylation of FOXO3 and FOXO3 upregulated PKIA expression through interacting with PKIA promoter. Moreover, SIRT1 silencing compromised dronedarone-mediated suppression of cell hypertrophy, while PKIA upregulation abolished the influences of SIRT1 silencing. More importantly, dronedarone improved TAC surgery-induced MH and impairment of cardiac function of rats via affecting SIRT1/FOXO3/PKIA axis. Conclusions: Dronedarone alleviated MH through mediating SIRT1/FOXO3/PKIA axis, which provide more evidences for dronedarone against MH.

• 지능정보연구
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• 제19권4호
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• pp.55-79
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• 2013

본 연구에서는 집중형 센터를 가진 역물류네트워크(Reverse logistics network with centralized centers : RLNCC)를 효율적을 해결하기 위한 혼합형 유전알고리즘(Hybrid genetic algorithm : HGA) 접근법을 제안한다. 제안된 HGA에서는 유전알고리즘(Genetic algorithm : GA)이 주요한 알고리즘으로 사용되며, GA 실행을 위해 0 혹은 1의 값을 가질 수 있는 새로운 비트스트링 표현구조(Bit-string representation scheme), Gen and Chang(1997)이 제안한 확장샘플링공간에서의 우수해 선택전략(Elitist strategy in enlarged sampling space) 2점 교차변이 연산자(Two-point crossover operator), 랜덤 돌연변이 연산자(Random mutation operator)가 사용된다. 또한 HGA에서는 혼합형 개념 적용을 위해 Michalewicz(1994)가 제안한 반복적언덕오르기법(Iterative hill climbing method : IHCM)이 사용된다. IHCM은 지역적 탐색기법(Local search technique) 중의 하나로서 GA탐색과정에 의해 수렴된 탐색공간에 대해 정밀하게 탐색을 실시한다. RLNCC는 역물류 네트워크에서 수집센터(Collection center), 재제조센터(Remanufacturing center), 재분배센터(Redistribution center), 2차 시장(Secondary market)으로 구성되며, 이들 각 센터 및 2차 시장들 중에서 하나의 센터 및 2차 시장만 개설되는 형태를 가지고 있다. 이러한 형태의 RLNCC는 혼합정수계획법(Mixed integer programming : MIP)모델로 표현되며, MIP 모델은 수송비용, 고정비용, 제품처리비용의 총합을 최소화하는 목적함수를 가지고 있다. 수송비용은 각 센터와 2차 시장 간에 제품수송에서 발생하는 비용을 의미하며, 고정비용은 각 센터 및 2차 시장의 개설여부에 따라 결정된다. 예를 들어 만일 세 개의 수집센터(수집센터 1, 2, 3의 개설비용이 각각 10.5, 12.1, 8.9)가 고려되고, 이 중에서 수집센터 1이 개설되고, 나머지 수집센터 2, 3은 개설되지 않을 경우, 전체고정비용은 10.5가 된다. 제품처리비용은 고객으로부터 회수된 제품을 각 센터 및 2차 시장에서 처리할 경우에 발생되는 비용을 의미한다. 수치실험에서는 본 연구에서 제안된 HGA접근법과 Yun(2013)의 연구에서 제안한 GA접근법이 다양한 수행도 평가 척도에 의해 서로 비교, 분석된다. Yun(2013)이 제안한 GA는 HGA에서 사용되는 IHCM과 같은 지역적탐색기법을 가지지 않는 접근법이다. 이들 두 접근법에서 동일한 조건의 실험을 위해 총세대수 : 10,000, 집단의 크기 : 20, 교차변이 확률 : 0.5, 돌연변이 확률 : 0.1, IHCM을 위한 탐색범위 : 2.0이 사용되며, 탐색의 랜덤성을 제거하기 위해 총 20번의 반복실행이 이루어 졌다. 사례로 제시된 두 가지 형태의 RLNCC에 대해 GA와 HGA가 각각 실행되었으며, 그 실험결과는 본 연구에서 제안된 HGA가 기존의 접근법인 GA보다 더 우수하다는 것이 증명되었다. 다만 본 연구에서는 비교적 규모가 작은 RLNCC만을 고려하였기에 추후 연구에서는 보다 규모가 큰 RLNCC에 대해 비교분석이 이루어 져야 할 것이다.

• 한국항행학회논문지
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• 제1권1호
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• pp.70-80
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• 1997

본 논문에서는 CDMA 이동전파 환경에서 두 가지 비동기식 동기 추적 회로 즉, TCTL과 MCTL의 성능을 비교, 분석한다. 먼저, 두 가지 방식에 대해 안정 상태에서의 지터 분산을 펄스 성형 필터, 타이밍 오프셋, 신호 대 잡음 비 및 루우프 대역폭의 함수로 이론적으로 유도한다. 또한, 루우프 필터의 설계 방법에 대해 2차 동기 추적회로를 중심으로 고찰한다. 끝으로, 동기 추적 오차에 의한 BER 성능 저하를 ETRI 에 의해 IMT-2000용으로 설계된 CDMA 시스템의 역방향 링크에 대해 분석한다.

• 한국의류학회지
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• 제17권2호
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• pp.301-306
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• 1993

Since the underquilt has an important role of supporting the human body in sleeping, it needs to sustain ample degrees of hardness, elasticity, humidity absorption, and warmth retention property and also to have the two ergonimical requirements : It should not be too soft to allow human bodies to sink in, and that it should be comfortable for humans to tum over in sleeping. This study aims to investigate the effect of the thermal insulation of the variation in weight applied to the underquilt. For this purpose, six items were selected as filling materials for the underquilt : cotton, wool, silk, down, polyester, cotton/ployester. Various weights were applied to each of the underquilts to survey the reduction tendency of its thermal insulation effect. The results are as follow : 1. The Thermal insulation effect of each underquilt decreased in an exponetial function as the weight on the underquilt was increased. 2. The thermal reduction curves according to the load weight insrease were shown to be constant in shape regardless of the weight increase. 3. At the weight of more than $25kg/m^2$ the degree of the thermal insulation effect of each underquilt was found to be in order of down>cotton>silk>polyester>wool>cotton/ployester. 4. The variation in load weight applied to each underquilt was shown to be in reverse correlation with the thermal insulation effect. An estimated regression formula can be made on the data.

• Bulletin of the Korean Chemical Society
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• 제33권2호
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• pp.489-491
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• 2012

An $^{125}I$-labeled resveratrol derivative 1 was synthesized. It was purified by reverse phase HPLC. Radiochemical purity of the product was more than 98%, and the yield was 35% (decay-corrected). Its biodistribution in tumorbearing mice was studied. The results showed that the highest radioactivity was located in intestine and stomach. The biodistribution profile suggests that compound 1 can be effectively used as a promising imaging probe for intestine and stomach.