• 상하수도학회지
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• 제33권5호
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• pp.395-404
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• 2019

The point-of-use water dispenser systems are widely used because of convenience in handling and demand for high-quality drinking water. The application has been increased recently in the public places such as department stores, universities and the rest areas in express ways. Improvement of water qualities by the dispenser systems was compared with tap water in this study. The tap water is supplied to the dispenser as the influent of the dispenser system. The twelve dispensers in the public places were used. The five dispensers used reverse osmosis as the main filter and other dispensers used various filters such as ultrafiltration, nanofiltration, and alumina filter. The water quality indicators for sanitation safety, i.e., turbidity and total coliforms, were evaluated. Other water qualities such as pH, residual chlorine, heterotrophic plate count (HPC), and total cell counts were also analyzed. By the point-of-use water dispenser, the turbidity, residual chlorine and pH were decreased and the HPC and total cell counts were increased. The t-test results revealed that the HPC of the tap waters were not significantly different from the treated waters but the total cell counts of the two groups were significantly different. The low pH of the RO filter treatment was also significantly different from the tap waters. This study will contribute to understand the role of the point-of-use water dispenser in improving water quality and to identify key water quality for the proper maintenance of the dispenser systems.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.67-67
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• 2021

The genus Glycyrrhiza (Licorice) has been used as an oriental herbal medicine for a long time in Asian countries. Wongam (WG), which is Glycyrrhiza new variety, have been developed to improve limitation of licorice including low productivity, environmental restriction and insufficient components by Korea Rural Development Administration. To using WG as a herbal medicine, it is important to reveal the adverse effects in health. In this study, we evaluated the genotoxicity test of WG extract through in vitro bacterial reverse mutation (AMES) assay, in vitro chromosomal aberration assay and in vivo mouse bone marrow micronucleus assay. When compared with the control, WG extract with or without the S9 mix showed no genotoxicity in the AMES assay up to 5000 ㎍/plate and in the chromosomal aberration assay up to 1100 ㎍/ml. In micronucleus assay, no significant increase in the number of micronucleated polychromatic erythrocytes or in the mean ratio of polychromatic to total erythrocytes up to 5000 mg/kg/day for 2 days. The present study demonstrated that WG extract is safe and reliable herbal medicine since no detectable genotoxic effects at least under the conditions of this study.

• 지구물리
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• 제3권3호
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• pp.141-152
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• 2000

남극반도 엘리펀트섬 북부의 남극-스코시아판 경계부에서 획득한 지구물리 자료(탄성파, 중력자료)는 셰클턴 파쇄대를 따라 지각구조의 급격한 변화를 보여준다. 이들 자료에 의하면 셰클턴 파쇄대의 해저산맥은 남극반도 대륙주변부 앞에서 중단되지만, 파쇄대의 변환단층대는 엘리펀트섬 부근의 주변부까지 계속 연장되며 그 넓이도 확장되고 있음을 보여준다. 즉 셰클턴 파쇄대의 변환단층은 남동쪽으로 내려오면서 (1) 드레이크 해협에서는 지구(graben) 구조의 함몰대, (2) 파쇄대 산맥의 남쪽 끝, 삼중점 바로 남동쪽에서는 해양지각에 대규모 반지구(half-graben) 구조, (3) 남극반도 엘리펀트섬 북쪽 대륙사면을 심하게 변형시키는 단층군으로 그 형태를 변화한다. 셰클턴 파쇄대의 단층대를 따라 두 단계의 판구조 환경 변화가 진행되었다. 첫 번째 단계는 대규모 정단층운동에 의해 반지구구조와 같은 확장구조를 형성시킨 확장력 환경이다. 이 시기는 드레이크 해협의 확장이 진행되었던 중기 마이오신세(약 $10Ma{\sim}20Ma$ 사이)에 해당된 것으로 추정된다. 두 번째 단계는 셰클턴 단층을 역단층운동으로 재활성화 시킨 최근의 압축력 환경으로, 이는 최근 약 6Ma이후 진행된 스코시아판의 서향운동으로 인한 남극판과의 수렴작용에 의한 것이다.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1114-1119
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• 2004

The biofilms on pipe walls in water distribution systems are of interest since they can lead to chlorine demand, coliform growth, pipe corrosion, and water taste and odor problems. As such, the study described in this paper is part of an AWWARF and Tampa Bay Water tailored collaboration project to determine the effect of blending different source waters on the water quality in various distribution systems. The project was based on 18 independent pilot distribution systems (PDS), each being fed by a different water blend (7 finished waters blended in different proportions). The source waters compared were groundwater, surface water, and brackish water, which were treated in a variety of pilot distribution systems, including reverse osmosis (RO) (desalination), both membrane and chemical softening, and ozonation-biological activated carbon (BAC), resulting in a total of 7 different finished waters. The observations from this study consistently demonstrated that unlined ductile iron was more heavily colonized by a biomass than galvanized steel, lined ductile iron, and PVC (in that order) and that the fixed biomass accumulation was more influenced by the nature of the supporting material than by the water quality (including the secondary residual levels). However, although the bulk liquid water cultivable bacterial counts (i.e. heterotrophic plate counts or HPCs) did not increase with a greater biofilm accumulation, the results also suggested that high HPCs corresponded to a low disinfectant residual more than a high biofilm inventory. Furthermore, temperature was found to affect the biofilms, plus the AOC was important when the residual was between 0.6 and 2.0 mg $Cl_2/l$. An additional aspect of the current study was that the potential of the exoproteolytic activity (PEPA) technique was used along with a traditional so-called destructive technique in which the biofilm was scrapped off the coupon surface, resuspended, and cultivated on an R2A agar. Both techniques indicated similar trends and relative comparisons among the PDSs, yet the culturable biofilm values for the traditional method were several orders of magnitude lower than the PEPA values.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2002년도 하계학술대회 논문집
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• pp.350-353
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• 2002

High voltage SiC Schottky barrier diodes with field plate structure have been fabricated and characterized. N-type 4H-SiC wafer with an epilayer of ∼10$\^$15/㎤ doping level was used as a starting material. Various Schottky metals such as Ni, Pt, Ta, Ti were sputtered and thermally-evaporated on the low-doped epilayer. Ohmic contact was formed at the backside of the SiC wafer by annealing at 950$^{\circ}C$ for 90 sec in argon using rapid thermal annealer. Field oxide of 550${\AA}$ in thickness was formed by a wet oxidation process at l150$^{\circ}C$ for 3h and subsequently heat-treated at l150$^{\circ}C$ for 30 min in argon for improving oxide quality. The turn-on voltages of the Ni/4H-SiC Schottky diode was 1.6V which was much higher than those of Pt(1.0V), Ta(0.7V) and Ti(0.7). The voltage drop was measured at the current density of 100A/$\textrm{cm}^2$ showing 2.1V for Ni Schottky diode, 1.45V for Pt 1.35V, for Ta, and 1.25V for Ti, respectively. The maximum reverse breakdown voltage was measured 1100V in the file plated Schottky diodes with 101an thick epilayer.

• Journal of Welding and Joining
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• 제27권6호
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• pp.31-35
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• 2009

When conducting CTOD test, especially in thick welded steel plate, fatigue pre-cracking occasionally failed to satisfy the requirements of standards thus making the test result invalid. Internally accumulated residual stress of test piece has been thought as one of the main reasons. The propagation of fatigue crack, started from the tip of machined notch, which might have propagated irregularly due to residual stress field. To overcome this kind of difficulty three methods to modify the residual stress are suggested in standard i.e. local compression, reverse bending and stepwise high-R ratio method. In this paper not only multi pass welding but also local pre-compressing process of thick steel plate has been simulated using finite element method for clarifying variation of internal welding residual stress. The simulated results show that welding residual stress is compressive in the middle section of the model and it is predominantly increased after machining the specimen. Comparing as-welded state all component of the welding residual stress changing to compressive in the tip of machine notch whereas residual stress of the outer area remain as tensile condition relatively. Analysis results also show that this irregular residual stress distribution is improved to be more uniformly by applying local compression.

• 대한토목학회논문집
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• 제26권3D호
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• pp.435-444
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• 2006

본 논문은 AREA법을 사용하는 줄눈 콘크리트포장의 평가에 3차원 유한요소모델을 사용하는 경우 나타나는 특성을 조사하는데 목적이 있다. 이를 위해 실제 콘크리트포장을 거동을 반영할 수 있는 3차원 유한요소모델을 구축하였고 2차원 모델과 비교한 후 민감도 분석을 수행하였다. ILLISLAB을 사용한 2차원 모델과 비교하였고 하중형태의 영향 보다 하중재하 지점의 자체수축과 기층모델에 따른 영향을 더 많이 받는 것으로 나타났다. 3차원 모델에서 비선형 온도구배를 선형 온도구배로 변화시키는 경우 발생하는 영향을 조사하였고 큰 차이를 보이지는 않았으나 지반 탄성계수가 작아질수록 더 많은 차이를 보였다. 동적하중에 의한 처짐을 구하였고 정적하중에 의한 처짐과 비교한 결과 낮은 지반 탄성계수에서는 동적하중에 의한 처짐이 작게 나타났으나 높은 지반 탄성계수에서는 동적하중에 의한 처짐이 크게 나타났다. 동적하중에 의한 처짐 이력을 구하고 AREA법을 이용하여 동적지지력과 탄성계수를 역산하였으며 이를 정적 처짐에 의한 결과와 비교하였다. 그 결과 동적지지력의 경우 정적 해석에 의한 값보다 동적해석에 의한 값이 낮게 나타났고 탄성계수의 경우 반대의 경향을 나타내어 현장에서 AREA법을 사용하는 경우 나타나는 평가결과의 특성을 설명하는 것으로 판단되었다.

• 한국산학기술학회논문지
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• 제17권12호
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• pp.120-127
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• 2016

본 연구에서는 수문의 설치방향에 따른 최적의 설치방향을 규명하기 위하여, 모형 제작에 의한 수문 진동실험을 수행하고, 진동특성이 연구되어진다. 47.5m 원형게이트가 아크릴을 사용하여 1:31 축척으로 제작되고, 납으로 질량이 보완된다. 먼저 모형을 검증하기 위하여 모형의 고유진동수가 측정되고, 원형 수문의 유한요소 해석 값으로 부터 환산된 고유진동수와 비교된다. 모형은 1.6m 폭을 갖는 콘크리트 수로에서 정방향과 역방향 설치에 따른 각각의 실험이 이루어 졌다. 각 방향 실험에서는 수문 개방고와 상하류 수위에 따른 여러 조건에서 수직진동이 측정되어 졌다. 실험결과를 바탕으로 정뱡향과 역방향 설치에 대한 진동현상을 서로 비교 분석하였다. 정방향 모형은 수문이 많이 열렸을 때 하단부의 수평트러스 부재를 치는 뒤돌아 치는 와류에 의하여 진동이 크게 발생하였고, 작은 개방고에서 suction force에 의한 진동이 발생하였다. 그러나 역방향 모형에서는 이러한 현상이 발생하지 않는다. 마지막으로 95m 수문의 진동실험결과와 본 47.m의 실험결과를 같이 비교하여 평가하였다. 실험결과 여러 실험 조건에서 진동이 작게 측정되는 역방향 배치 모형이 더 합리적인 것으로 분석되었다.

• The Plant Pathology Journal
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• 제26권1호
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• pp.25-31
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• 2010

Papaya ringspot virus (PRSV) causes the most widespread and devastating disease in papaya. Isolates of PRSV originating from different geographical regions in south India were collected and maintained on natural host papaya. The entire coat protein (CP) gene of Papaya ringspot virus-P biotype (PRSV-P) was amplified by RTPCR. The amplicon was inserted into pGEM-T vector, sequenced and sub cloned into a bacterial expression vector pRSET-A using a directional cloning strategy. The PRSV coat protein was over-expressed as a fusion protein in Escherichia coli. SDS-PAGE gel revealed that CP expressed as a ~40 kDa protein. The recombinant coat protein (rCP) fused with 6x His-tag was purified from E.coli using Ni-NTA resin. The antigenicity of the fusion protein was determined by western blot analysis using antibodies raised against purified PRSV. The purified rCP was used as an antigen to produce high titer PRSV specific polyclonal antiserum. The resulting antiserum was used to develop an immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) assay and compared its sensitivity levels with ELISA based assays for detection of PRSV isolates. IC-RT-PCR was shown to be the most sensitive test followed by dot-blot immunobinding assay (DBIA) and plate trapped ELISA.

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1258-1266
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• 2005

The present study was carried out in order to assess the protective effects of calycosin-7-O-$\beta$-D-glucopyranoside, isolated from Astragali radix (AR), on hyaluronidase (HAase) and the recombinant human interleukin-$1\beta$ (IL-$1\beta$)-induced matrix degradation in human articular cartilage and chondrocytes. We isolated the active component from the n-butanol soluble fraction of AR (ARBu) as the HAase inhibitor and structurally identified as calycosin-7-O-$\beta$-D-glucopyranoside by LC-MS, IR, ${1}^H$ NMR, and ${13}^C$ NMR analyses. The $IC_{50}$ of this component on HAase was found to be 3.7 mg/ml by in vitro agarose plate assay. The protective effect of ARBu on the matrix gene expression of immortalized chondrocyte cell line C28/I2 treated with HAase was investigated using a reverse transcription polymerase chain reaction (RT-PCR), and its effect on HAase and IL-$1\beta$-induced matrix degradation in human articular cartilage was determined by a staining method and calculating the amount of degraded glycosaminoglycan (GAG) from the cultured media. Pretreatment with calycosin-7-O-$\beta$-D-glucopyranoside effectively protected human chondrocytes and articular cartilage from matrix degradation. Therefore, calycosin-7-O-$\beta$-D-glucopyranoside from AR appears to be a potential natural ant-inflammatory or antii-osteoarthritis agent and can be effectively used to protect from proteoglycan (PG) degradation.