• Bulletin of the Korean Chemical Society
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• 제14권4호
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• pp.510-514
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• 1993

The retention behavior of proteins was investigated by using reversed-phase chromatography (RPC), comparing to the retention behavior of small molecules in RPC. The evaluation was carried out on a SynChropak RP-P($C_{18}$) column with 0.1% aq. TFA-organic solvent modifier such as acetonitrile, isopropanol, and ethanol. The Z value (the number of solvent molecules required to displace the solute from the surface) was a general index for the characterization of protein retention as a function of organic concentration over a range of temperature between 5 and 70$^{\circ}C$. Van't Hoff plots provided the basis for evaluating the enthalpic and entropic changes associated with the interaction between protein and the stationary phase. Z values did not change significantly at the range of temperature showing the consistent ${\Delta}H^{\circ}$ and ${\Delta}S^{\circ}$ values. From these investigation, it was concluded that the retention behavior of proteins in RPC was able to be predicted by the retention parameters applied to small molecules. Furthermore, myoglobin and hemoglobin in RPC as stated above showed a similar retention behavior regardless of their molecular weights.

• 대한환경공학회지
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• 제29권2호
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• pp.229-234
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• 2007

본 연구에서는 고형물 체류시간, 수리학적 체류시간, 생흡착시간 및 온도 변화가 이단슬러지 유형의 연속회분식반응기 공정 운영에 어떻게 영향을 주는지를 조사함으로서 본 공정을 최적화 하고자 하였다. T-N 제거에 있어서 고형물 체류시간이 증가할수록 T-N 제거효율이 증가하는 경향을 관찰할 수 있었는데 이는 SRT가 증가할수록 SCOD 생흡착효율의 증가와 관련이 있을 것으로 판단된다. HRT 영향에 있어서 HRT 8시간, 10시간 및 15시간에서 암모니아성 제거효율 및 T-N 제거효율은 각각 HRT 영향에 관계없이 거의 같았다. 생흡착시간을 20분 이상 증가시켜도 T-N 제거효율 향상에는 도움이 되지 않는 것으로 관찰되었다. 서로 다른 온도 조건에서 공정 제거 효율 비교에 관해 조사한 결과 온도의 감소가 공정 성능에 영향을 주지는 않았으나 인 제거 효율에 있어서 높은 온도에서보다 낮은 온도에서 인 제거효율이 다소 높게 관찰되었다. 결과적으로 본 연구에서 개발된 공정은 낮은 온도 조건 및 높은 유입 부하를 가진 폐수처리에 적합한 것으로 나타났다.

• Membrane and Water Treatment
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• 제10권2호
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• pp.179-189
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• 2019

This study aimed to investigate the effect of temperature and solid retention time (SRT) on membrane fouling in a membrane bioreactors (MBRs). For this purpose, a lab-scale submerged MBR system was used. This system operated at two SRTs of 15 and 5 days, three various temperatures (20, 25 and $30^{\circ}C$) and hydraulic retention time (HRT) of 8 h. The results indicated that decreased the cake layer resistance and increased particles size of foulant due to increasing temperature and SRT. Fourier transform infrared (FTIR) analysis show that the cake layer formed on the membrane surface, contained high levels of proteins and especially polysaccharides in extracellular polymeric substances (EPS) but absorbance intensity of EPS functional groups decreased with temperature and SRT. EEM analysis showed that the peak on the range of Ex/Em=220-240/350-400 in SRT of 15 and temperature of $30^{\circ}C$ indicates the presence of fulvic acid in the cake. In addition, as the temperature rise from 20 to $30^{\circ}C$, concentration of soluble microbial products (SMP) increased and COD removal reached 89%. Furthermore, the rate of membrane fouling was found to increase with decreasing temperature and SRT.

• Bulletin of the Korean Chemical Society
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• 제14권4호
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• pp.515-519
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• 1993

The retention behavior of proteins was investigated by using hydrophobic interaction chromatography (HIC), comparing to the results obtained in reversed-phase chromatography (RPC) described in the previous paper. A SynChropak propyl column was employed with 0.05 M phosphate buffer (pH 7.0) containing sodium sulfate. Conformational changes were recognized by examining Z values as a function of sodium sulfate concentration over a range of temperature between 5 and 65$^{\circ}C$. Z values did not change significantly at the range of the temperature showing the consistent ${\Delta}H^{\circ}$ and ${\Delta}S^{\circ}$ values. The sign and the magnitude of ${\Delta}H^{\circ}$ and ${\Delta}S^{\circ}$ of proteins in HIC were compared with those obtained in RPC. The signs of ${\Delta}H^{\circ}$ and ${\Delta}S^{\circ}$ of proteins in HIC were all positive, while those of proteins in RPC were all negative. These results suggested that the retention of proteins in HIC and in RPC were entropy-driven and enthalpy-driven process, respectively. From the two different investigations, it was concluded that the retention mechanism of RPC and HIC was based on the same fundamental principle in which separation is dependent on hydrophobicity, but the retention behavior of the proteins in HIC is clearly different from that observed in RPC.

• 환경위생공학
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• 제11권3호
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• pp.13-19
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• 1996

Packed bed reactor containing immobilized microorganisms which degraded phenol without growth was used to remove phenol from the synthetic wastewater. The effects of temperature, retention time(reactor volume/flow rate) and phenol concentration on the removal efficiency of phenol were investigated. The effect of temperature in the range of 20-30$\circ $C was negligible while retention time and phenol concentration influenced the removal of phenol significantly. When retention time was in the range of 1-1.5 hour, the removal efficiency of phenol was affected not by phenol concentration but by retention time itself while it was influenced by phenol concentration above 1.5 hour of retention time. The beads after 720 hours operation were swelled by 40 % in diameter which could be prevented by crosslinking with glutaraldehyde at the expense of cell activity.

• 한국해양바이오학회지
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• 제11권2호
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• pp.89-93
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• 2019

In this study, the stability of the extracted natural pigments against light, temperature, pH, metal ions, and antimicrobial activity were evaluated in marine bacteria Zooshikella sp. 17TA. The pigment of the strain used in the study was red with maximum absorption at a wavelength of 541 nm. The stability of the pigment was evaluated by measuring the absorbance while preserving for 15 days and examining the retention rate. After 15 days of irradiation, the pigment of this bacterium showed 98% retention in the dark and 91% retention in the temperature range of -20℃ ~ 30℃. When the pH was in the range 4-7, the retention was about 80%, and the retention rate was higher than 85% for all kinds of metal ions except for CuCl₂, ZnCl₂, and KCl. The bacterial pigments showed high stability under the given irradiated pH, temperature, and metal ion conditions and had shown activity against gram-positive strains. These results suggest that this highly conserved microbial pigment can be applied to the food industry.

• Journal of Animal Science and Technology
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• 제61권3호
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• pp.170-181
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• 2019

This study aimed to investigate the effects of temperature and retention time of the pressurized steam chamber on the ruminal fermentation characteristics and nutrient degradability of corn flakes in three Korean native Hanwoo cows and three Holstein cows implanted with a ruminal fistula. Corn kernels were categorized into 13 groups based on the chamber temperature (range, $100^{\circ}C-116^{\circ}C$) and retention time (range, 700-950 s). The pH value was lowest in T1 regardless of breed. Propionate concentration was the highest in T2 (p < 0.05). Total-volatile fatty acid (VFA) concentration was slightly but not significantly greater in T2 than in other conditions. Dry matter (p < 0.05), starch, and crude protein (p < 0.05) degradability were the highest in T1. At different incubation times and with different breeds, dry matter, starch, and crude protein degradability of corn flakes were the highest in T1. Thus, the present results indicate that the optimal temperature and retention time of the pressurized steam chamber should be $100^{\circ}C-105^{\circ}C$ and 700-720 s.

• Advances in materials Research
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• 제4권1호
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• pp.13-22
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• 2015

Concrete suffers strength loss when subjected to elevated temperatures during an accidental event such as fire. The loss in strength of concrete is mainly attributed to decomposition of C-S-H gel and release of chemically bound water, which begins when the temperature exceeds $500^{\circ}C$. But it is unclear about how much strength loss occurs in different stages of heating, retention and cooling regimes. This work is carried out to separate the total strength loss into losses during different stages of heating, retention and cooling. Tests were carried out on both Ordinary Portland Cement (OPC) based concrete and Ground Granulated Blast Furnace Slag (GGBFS) blended concrete for $200^{\circ}C$, $400^{\circ}C$, $600^{\circ}C$ and $800^{\circ}C$ with a retention period of 1 hour for each of these temperature levels. Furnace cooling was adopted throughout the experiment. This study reports strength loss contribution during heating, retention and cooling regimes for both OPC based and GGBFS based concretes.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1991년도 추계학술대회 논문집
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• pp.44-47
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• 1991

Electron injection and memory retention chracteristics of the MNOS devices with thin oxide layer of 23${\AA}$ thick and silicon nitride layer of 1000${\AA}$ thick which are fabricated for this experiment. As a result, pulse amplitude increase oxide current is dominated in linearly increasing region of $\Delta$V$\_$FB/the decreasing region after saturation was due to the increased silicon nirtide current. In low pulse ampiltude $\Delta$V$\_$FB/ is not variated on temperature, but as temperature and pulse amplitude increase. $\Delta$V$\_$FB/ is decreased after saturation. And the decay rate during 10$^4$sec after electron injection was ohiefly dominated by the back tunneling of emission from memory trap to silicon. Memory retention characteristics in V$\_$FB/ stage was better than that of OV retention regardless of injection conditions.

• Current Research on Agriculture and Life Sciences
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• 제11권
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• pp.1-9
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• 1993

신문 용지용(用) 지료(紙料)의 조성조건(調成條件)에 따른 보류(保留)의 변화를 연구(硏究)한 결과 다음과 같은 사실을 알수가 있었다. 1. 온도(溫度)의 상승(上昇)에 따라 보류(保留)는 지료(紙料)의 종류 및 배합비(配合比)에 관계없이 감소(減少)하였으며 GP보다 DIP가 온도(溫度)변화에 민감(敏感)하였다. 2. pH변화에 따른 보류(保留)의 변화는 GP와 DIP 및 GP/DIP=50/50 모두 pH5에서 보류(保留)가 가장 높았으며 pH 5보다 높거나 낮으면 감소(減少)하였고 DIP가 GP보다 감소(減少)가 적었다. 3. Alum첨가(添加)에 따른 보류(保留)의 변화는 GP와 GP/DIP=50/50의 경우 2% 첨가(添加)에서 보류(保留)가 가장 높았고 DIP는 3% 첨가(添加)에서 가장 높았다. 4. 보류(保留)는 지료(紙料)의 응집작용(凝集作用)과 밀접한 관련이 있었다.