• Journal of the Korean Society of Food Science and Nutrition
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• 제24권6호
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• pp.1016-1019
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• 1995

This study was conducted to investigate the nutrient composition of hydroponic water dropwort and the effect of blanching condition on ascorbic acid content. Nutrient composition of hydroponic water dropwort of mid harvest on the 30th day was measured in three portions of leaves, petioles and stems, and was compared with that of late harvest on the 40th day. Hydroponic water dropwort was obtained from the Department of Horticultural Science, Seoul National University. The results were as follows. The nutrients content of leaves were significantly higher in ash and ascorbic acid and lower in moisture, crude fat and crude fiber than those of stems and petioles. There was no significant difference in total vitamin A and thiamin contents between three portions. Ascorbic acid content of leaves harvested on the 30th day was the highest, 57mg/100g, and decreased to 18mg/100g in the leaves harvested on the 40th day. Marked increase in crude fiber and vitamin A content of leaves was observed in late harvest may be due to the maturation. The results of nutrient composition analysis suggest that the leaves of hydroponic water dropwort is important in ascorbic acid and ash. It is recommended that shorter blanching time and addition of 0.5% NaCl to the blanching water are better for higher ascorbic acid retention of hydroponic water dropwort. In conclusion, as hydroponic water dropwort has high content in ash, calcium, vitamin and free sugar with alkalinity, leaves as well as stems and petioles can be recommonded as a vegetable of high nutritional quality.

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.349-354
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• 2004

The effects of probiotic-vitacogen and galacto-oligosaccharides (GOS) supplementation on methanogesis, energy and nitrogen utilization in replacement dairy cows were evaluated. A basal diet comprising orchardgrass hay, lucerne hay cube and concentrate (2:2:1, DM basis) were fed with or without supplements to four cows at $80g\;DM/kgBW^{0.75}$per day in a $4{\times}4$ Latin square arrangement. The four treatments were; 1) basal diet, 2) basal diet plus 100 g probiotic-vitacogen, 3) basal diet plus 50 g GOS, 4) basal diet plus 50 g GOS and 100 g probiotic-vitacogen. Nutrient apparent digestibility was not altered by the effect of supplementation. Nitrogen intake was significantly (p<0.001) higher for the two vitacogen-supplemented diets compared to control and GOS supplemented diets. However, vitacogen supplemented diets had numerically higher fecal and urinary nitrogen losses, thereby, having lower nitrogen retention compared to control and GOS supplemented diets. Gross energy intake was also significantly (p<0.05) higher for vitacogen-supplemented diets compared to control and GOS diets, however, due to higher losses in feces, urine, methane and heat, GOS supplemented diet had numerically higher energy retention. There was an 11% reduction in methane emission (liters/day) in GOS supplemented diet compared to control diet. However, the combination of GOS with vitacogen resulted in an increased methane emission. When expressed per unit of animal production (g/kg live-weight gain), methane production tended to be lower in vitacogensupplemented diets compared to control and GOS diets. The supplementation of replacement dairy cows with GOS reduced methane emission (liters/day), while, vitacogen supplementation reduced methane emission per unit animal production. The two feed supplements may contribute to the abatement of methane as a greenhouse gas.

• Journal of Physiology & Pathology in Korean Medicine
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• 제25권2호
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• pp.300-305
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• 2011

The present study was investigated the neuroprotective effects of acupuncture at ST36 on learning and memory deficits after transient cerebral ischemia in a gerbil model. The animals were randomly divided into three groups (n=7 in each group): the sham operation group (SHAM), ischemia-induced and ST36 acupuncture group (ISC + ST36), and the ischemia-induced and Tail-acupuncture group (ISC + TAIL). For the acupuncture stimulation, stainless steel needles, 0.3 mm in diameter, were inserted bilaterally into the ST36 locus or the tail and stimulated for 1 min/day for 14 days. Using the Morris water maze test, the animals were tested on spatial learning and memory. In addition, the effects of acupuncture on memory storage and the choline acetyltransferase (ChAT) activity, in the hippocampal CA1 area, were investigated by ChAT immunohistochemistry. Transient cerebral ischemia produced impaired performance on the MWM test (DAY 5: p<0.01 and retention test: p<0.05) and severely decreased ChAT immunoreactivity in the CA1 hippocampal area compared to the SHAM group (p<0.05). However, improved learning and memory were observed (DAY 5: p<0.05 and retention test: p<0.01) as well as a significantly reduced loss of ChAT immunoactivity in the hippocampal CA1 region (p<0.001) after acupuncture stimulation at ST36 were observed. These results show that acupuncture at ST36 ameliorated the learning and memory deficits at least in part through the cholinergic system. The findings of this study provide potential data that acupuncture is useful for the treatment of some of the behavioral impairs of transient cerebral ischemia.

• Korean Journal of Poultry Science
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• 제34권2호
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• pp.143-149
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• 2007

The experiment was conducted to examine the effects of dietary organic selenium and vitamin E on weight gain, feed intake, feed conversion, and selenium retention in meat of broiler chickens. For each growth phase, the basal diet was supplemented with 0 (control), vitamin E 150 IU/kg and the combination of 1.2 ppm Se from selenium yeast (SY) and vitamin E 100, 150, 200 and 300 IU/kg. Weight gain was significantly higher in supplemental control and vitamin E 150 compared to the combination of 1.2 ppm SY and vitamin E 150 IU during day 1 to 21. Feed intake significantly(P<0.05) increased in supplemental vitamin E 150 compared to the combination of 1.2 ppm SY and vitamin E 150 and 200 IU during day 1 to 21. Feed intake was significantly(P<0.05) higher in control compared to that of combination of 1.2 ppm SY and vitamin E 200 IU during day 21 to 35. However feed conversion was not affected in supplemental vitamin E and SY during day 1 to 35. Selenium concentrations of breast muscle and liver tissue significantly increased (P<0.05) in supplemental combination of 1.2 ppm SY and vitamin E compared to the control and vitamin E 150 IU. TBARS of control and vitamin E 150 IU were significantly (P<0.05) higher in day 3 than day 1, but the combination of Se 1.2 ppm and vitamin E of TBARS had no difference during day 1 to 3. TBARS in day 3 was significantly (P<0.05) lower in supplemental combination of Se 1.2 ppm and vitamin E than control and vitamin E 150 IU.

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1580-1587
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• 2006

Several researchers have demonstrated that the rumen microbial community rapidly adapts to saponins and proposed interval feeding to prevent this rapid adaptation. An in vivo experiment was carried out to examine the effect of daily versus application every third day (interval feeding) of Sapindus rarak saponins (SE) on rumen fermentation end products, protozoal counts and nutrient digestibility. Thirty sheep were allocated into 5 groups. Sheep were fed daily or every third day with two levels of SE (0.48 and 0.72 g/kg body mass). One group received no saponin and served as control. All sheep received the same diet, a mixture of elephant grass and wheat pollard (65:35 w/w). Independent of the feeding regime and the level of inclusion, the addition of SE decreased protozoal counts and rumen ammonia concentrations (p<0.01). Microbial N supply and N retention were not affected by the high feeding regime. Daily feeding negatively influenced rumen xylanase and cellulase activity, but only when the high level of saponins was fed. However, these negative effects on rumen cell wall degradation were not reflected in decreasing total tract digestibility of the organic matter or the plant cell walls. Our results show that rumen microorganisms do not rapidly adapt to S. rarak saponins.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.537-540
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• 2001

A parallel TBF system that is consisted of two TBFs was developed f ‘ or the long-term treatment of gas-phase trichloroethylene (TCE). Each TBF was operated for TCE degradation or reactivation in a parallel mode, and the effect of switching time and operation variables between the two reactors was investigated. Within 12 hr after switching from TCE degradation to reactivation mode, the MO activity increased up to the initial level. More than 50 % of TCE was degraded for feed concentrations ranging from 5 to 17 ppmv, and completely 100 % removed at concentration of less than 5 ppmv, while TCE removal decreased severely over 28 ppmv. In various empty bed retention times (EBRTs), ranging 상 om 5.2 to 10.7 min, the optimal EBRT was 10.7 min that TCE conversion achieved more than 50 %. For the inlet loading below 23.4 mg TCE/L/day, TCE was entirely removed. The maximal TCE elimination capacity in this system was about 66.63 mg TCE/L/day. During the continuous treatment of TCE over 3 months, TCE removal efficiency was maintained at the range of about 50 %. In these results, the parallel TBF system can be available for the continuously TCE biodegrading operation.

• Korean Journal of Pharmacognosy
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• 제48권1호
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• pp.77-81
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• 2017

The aim of this study was to investigate a validation method for determination of tricin in Phragmites communis ears. Tricin was isolated with chromatographic methods and used as the standard substances for quantitative analysis. The structural determination was characterized by comparing their NMR spectral data with values reported in the literature. For validation, the specificity, linearity, precision, accuracy, detection limits, and quantification limits of tricin was measured by HPLC. The results show that the specificity was satisfied with retention time and diode array detector (DAD) spectrum by analysis of tricin using HPLC. The limits of detection (LOD) for tricin was 0.84 mg/ml. Recovery of tricin was 98.80~108.22% with R.S.D values less than 2%. Intra-day and inter-day precisions of tricin in P. communis ears were 99.96~100.96% and 99.01~100.40%, respectively. Therefore, application of tricin was validated by an analytical method as major compound in P. communis ears.

• Nutritional Sciences
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• 제2권2호
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• pp.76-81
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• 1999

Sodium restricted diets are known to lower blood pressure in salt sensitive, hypertensive patients. Thare is increasing evidence that potassium plays an important role as a protective factor in the regulation of blood pressure. The objective of the current study was to measure parameters of sodium, potassium, and chloride utilization as affected by feeding of substantial quantities of bread made from whole ground white torn meal, whole ground yellow corn meal, and whole ground wheat flour. The breads provided 40 percent of a caloric content of the constant, measured laboratory diet. The 28-day study was divided into an introductory period of 7-days and three experimental periods of 7-days each. Order of assignment to specific treatments for 12 healthy subjects were according to a complete randomized block design. Yellow corn bread diets resulted in the highest potassium retention (243 mg/day) and the lowest urinary sodium and potassium ratio (1.53 $\pm$ 0.26) numerically in comparison to the other test breads. The excretions of sodium and chloride were higher during controlled feeding periods than during the self-selected diet period(p < 0.05). This indicates a response to the higher intake of these electrolytes from the experimental diets than from self-selected diets. There was no significant difference in the effect of white corn bread, yellow corn bread, or whole wheat bread diet on electrolyte status in humans. However, the yellow corn bread diet resulted in a somewhat more favorable urina교 sodium to potassium ratio than that from white corn bread or whole wheat bread diet.

• Journal of Nutrition and Health
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• 제31권1호
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• pp.5-12
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• 1998

This study was designed to examine how dietary protein levels affect organ growth and protein metabolism in early and normally weaned rats. Early and normally weaned rats separated fro the dam on the 15th and 121st day postpartum, respectively. were fed diets containing three levels of protein : low(10%) , normal (20%),and high(40%) . On the 35th day, the weight and DNA, RNA and protein contents in brain , liver, and kidney were determined to ascertain organ and cellular growth. Furthermore, serum total protein , albumin , $\alpha$-amino N and creatine and urinary urea N, and creatinine were determined in order to ascertain protein metabolism and renal functions. Dietary protein levels were not observed to significantly affect total DNA content, which may represent an index of cell number in the liver, brain and kidney. Fresh weight and protein/DNA ratio, which may represent indices of cell size, significantly increased in proportion to dietary protein in the kidney. As for the early weaned rats , the liver cell size significantly decreased. Dietary protein levels and weaning periods did not affect serum total protein and albumin . However, serum urea-N significantly increased in proportion to dietary protein levels whereas serum $\alpha$-amino N was decreased by early weaning . Nitrogen retention was lower in early weaned rats fed low or high levels of protein than in normally weaned rats. The results demonstrate that low or high levels of dietary protein have less desirable effects on protein metabolism in prematurely weaned rats.

• Asian-Australasian Journal of Animal Sciences
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• 제8권5호
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• pp.443-447
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• 1995

Fifteen Karan-Swiss male calves of 9-12 months of age were divided into three groups of five each in a randomised block design. Animals in group I were fed wheat straw ad lib. and concentrate mixture according to their requirements, while in group II and III the animals were fed with 1/3 of the required concentrate mixture replaced by UMM licks 'Ex' and 'F', respectively. The DMI (kg/day as well as per 100 kg b.wt.) were similar (p > 0.05) among different groups of animals, however, the digestibility of DM as well as OM enhanced from $52.85{\pm}1.48$ to $58.36{\pm}1.89$ and $55.33{\pm}1.48$ to $60.12{\pm}1.75$, respectively. Growth rates of the calves were $533.8{\pm}27.25$, $532.3{\pm}42.24$ and $538.4{\pm}18.68$ g/d in groups I, II and ill (p > 0.05), respectively. Body composition and N balances of the animals were not affected by supplementation of UMM licks, however, protein retention efficiency was higher in group III ($82.57{\pm}2.54$) though nonsignificant. Feed cost/day was reduced from Rs. 7.92 (group I) to Rs. 4.62 (group II) and Rs. 3.44 (group III). Hence, partial replacement of concentrates by UMM licks reduced the cost of feeding of growing calves by 41.7 to 56.6% without affecting the growth performance.