Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Isolation and Quantitative Analysis of Tricin from Ears of Phragmites communis

갈대이삭으로부터 Tricin의 분리 및 함량 분석

  • Woo, Hyun Sim (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Seung-Young (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Hwang, Buyng Su (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Jeong, Sang-Chul (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources) ;
  • Kim, Dae Wook (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
  • 우현심 (국립경상대학교 응용생명과학부) ;
  • 이승영 (국립낙동강생물자원관 담수생물특성연구실) ;
  • 황병수 (국립낙동강생물자원관 담수생물특성연구실) ;
  • 정상철 (국립낙동강생물자원관 담수생물특성연구실) ;
  • 김대욱 (국립낙동강생물자원관 담수생물특성연구실)
  • Received : 2016.12.22
  • Accepted : 2017.03.20
  • Published : 2017.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to investigate a validation method for determination of tricin in Phragmites communis ears. Tricin was isolated with chromatographic methods and used as the standard substances for quantitative analysis. The structural determination was characterized by comparing their NMR spectral data with values reported in the literature. For validation, the specificity, linearity, precision, accuracy, detection limits, and quantification limits of tricin was measured by HPLC. The results show that the specificity was satisfied with retention time and diode array detector (DAD) spectrum by analysis of tricin using HPLC. The limits of detection (LOD) for tricin was 0.84 mg/ml. Recovery of tricin was 98.80~108.22% with R.S.D values less than 2%. Intra-day and inter-day precisions of tricin in P. communis ears were 99.96~100.96% and 99.01~100.40%, respectively. Therefore, application of tricin was validated by an analytical method as major compound in P. communis ears.

Keywords

References

  1. Baxter, H. and Harborne, J. B. (1999) The Handbook of Natural Flavonoids. John wiley & Sons, New York.
  2. Martens, S. and Mithofer, A. (2005) Flavones and flavone synthases. Phytochemistry 66: 2399-2407. https://doi.org/10.1016/j.phytochem.2005.07.013
  3. Lu, B., Wu, X., Tie, X. and Zhang, Y. (2005) Toxicology and safety of anti-oxidant of bamboo leaves. Part 1: Acute and subchronic toxicity studies on anti-oxidant of bamboo leaves. Food Chem. Toxicol. 43: 783-792. https://doi.org/10.1016/j.fct.2005.01.019
  4. Verschoyle, R. D., Greaves, P., Cai, H., Borkhardt, A., Broggini, M., D'lncalci, M., Riccio, E., Doppalapudi, R., Kapetanovic, I. M., Steward, W. P. and Gescher, A. J. (2006) Preliminary safety evaluation of the putative cancer chemopreventive agent tricin, a naturally occurring flavone. Cancer Chemother. Pharmacol. 57: 1-6. https://doi.org/10.1007/s00280-005-0039-y
  5. Chang, C. L., Zhang, L. J., Wu, C. C., Huang, H. C., Roy, M. C., Huang, J. P., Wu, Y. C. and Kuo, Y. H. (2010) Quiquelignan A-H, eight new lignoids from the rattan palm Calamus quiquesetinervius and their antiradical, anti-inflammatory and antiplatelet aggregation activities. Bioorg. Med. Chem. 18: 518-525. https://doi.org/10.1016/j.bmc.2009.12.016
  6. Ju, Y., Sacalis, J. N. and Still, C. C. (1998) Bioactive flavonoids from endophyte-infected blue grass (Poa ampla). J. Agric. Food Chem. 46: 3785-3788. https://doi.org/10.1021/jf980189m
  7. Harborne, J. B. and Hall, E. (1964) Plant polyphenols-XII. : The occurrence of tricin and of glycoflavones in grasses. Phytochemistry 3: 421-428. https://doi.org/10.1016/S0031-9422(00)83627-4
  8. Li, M., Pu, Y., Yoo, C. G. and Ragauskas, A. J. (2016) The occurrence of tricin and its derivatives in plants. Green Chem. 18: 1439-1454. https://doi.org/10.1039/C5GC03062E
  9. Jung, G. E. and Lee, J. S. (2011) Dyeability and functionality of bamboo extracts (Part I)-Characteristics of bamboo extracts and dyeing properties of cotton. J. Korean Soc. Cloth. Text. 35: 206-217. https://doi.org/10.5850/JKSCT.2011.35.2.206
  10. Lee, D., Park, H. Y., Kim, S., Park, Y., Bang, M. H. and Imm, J. Y. (2015) Anti-adipogenic effect of oat hull extract containing tricin on 3T3-L1 adipocytes. Process Biochem. 50: 2314-2321. https://doi.org/10.1016/j.procbio.2015.09.019
  11. Kim, C. M., Shin, M. K., Ahn, D. K. and Lee, K. S. (1998) A compete translation dictionary of China medicine. Jeongdam Publisher. Seoul Korea.
  12. Shin, K. Y., Lee, G. H., Park, C. H., Kim, H. J., Park, S. H., Kim, S., Kim, H. S., Lee, K. S., Won, B. Y., Lee, H. G., Choi, J. H. and Suh, Y. H. (2007) A novel compound, maltolyl pcoumarate, attenuates cognitive deficits and shows neuroprotective effects in vitro and in vivo dementia models. J. Neurosci. Res. 85: 2500-2511. https://doi.org/10.1002/jnr.21397
  13. Choi, S. E., Yoon, J. H., Park, K. H., Kim, K. Y., Song, Y. J., Jin, H. Y. and Lee, M. W. (2014) Whitening activity of phenolic compounds from rhizome of Phragmites communis. Nat. Prod. Sci. 20: 269-273.
  14. Bhattacharyya, J., Stagg, D., Mody, N. V. and Miles, D. H. (1978) Constituents of Spartina cynosuroides: isolation and $^{13}C$-NMR analysis of tricin. J. pharm. sci. 67: 1325-1326. https://doi.org/10.1002/jps.2600670938