• Journal of Pharmaceutical Investigation
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• 제34권1호
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• pp.1-14
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• 2004

A promising approach to the development of a new single-step vaccine, which would eliminate the requirement for multiple injections, involves the encapsulation of antigens into microspheres. Biodegradable poly(lactide-co-glycolide) (PLGA) microspheres gave us a bright insight for controling antigen release in a pulsatile fashion, thereby mimicking two or tree boosting injections. However, in spite of the above merits, the level of immunization induced by a single-shot vaccination is often lower tan two doses of alum-adsorbed antigen. Therefore, optima modification of the microsphere is essential for the development of single-step vaccines. In the review, we discuss the stability of antigen in microsphere, safety and non-toxic in human and encapsulation technology. Also, we attempted to outline relevant physicochemical properties on the immunogenicity of microsphere vaccine and attainment of pulsatile release pater by combination of different microsphere, as well as to analyze immunological data associated with antigen delivery by microsphere. Although a lot of variables are related to the optimized microsphere formulation, we could conclude that judicious choice of proper polymer type, adjustment of particles size, and appropriate immunization protocol along with a suitable adjuvant might be a crucial factor for the generation of long-lasting immune response from a single-step vaccine formulation employing PLGA microsphere.

• Earthquakes and Structures
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• 제12권2호
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• pp.251-262
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• 2017

The present research intends to study the effects of the seismic soil-foundation-structure interaction (SFSI) on the dynamic response of various buildings. Two methods including direct and Cone model were studied through 3D finite element method using ABAQUS software. Cone model as an approximate method to consider the SFSI phenomenon was developed and evaluated for both high and low rise buildings. Effect of soil nonlinearity, foundation rigidity and embedment as well as friction coefficient between soil-foundation interfaces during seismic excitation are investigated. Validity and performance of both approaches are evaluated as reference graphs for Cone model and infinite boundary condition, soil nonlinearity and amplification factor for direct method. A series of calculations by DeepSoil for inverse earthquake record modification was conducted. A comparison of the two methods was carried out by root-mean-square-deviation (RMSD) tool for maximum lateral displacement and story shear forces which verifies that Cone model results have good agreement with direct method. It was concluded that Cone method is a convenient, fast and rather accurate method as an approximate way to count for soil media.

• BMB Reports
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• 제45권10호
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• pp.554-559
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• 2012

Oligosaccharide modification by N-acetylglucosaminyltransferase-V (GnT-V), a glycosyltransferase encoded by the Mgat5 gene that catalyzes the formation of ${\beta}1$,6GlcNAc (N-acetylglucosamine) branches on N-glycans, is thought to be associated with cancer growth and metastasis. Overexpression of GnT-V in cancer cells enhances the signaling of growth factors such as epidermal growth factor by increasing galectin-3 binding to polylactosamine structures on receptor N-glycans. In contrast, GnT-V deficient mice are born healthy and lack ${\beta}1$,6GlcNAc branches on N-glycans, but develop immunological disorders due to T-cell dysfunction at 12-20 months of age. We have developed Mgat5 transgenic (Tg) mice (GnT-V Tg mice) using a ${\beta}$-actin promoter and found characteristic phenotypes in skin, liver, and T cells in the mice. Although the GnT-V Tg mice do not develop spontaneous cancers in any organs, there are differences in the response to external stimuli between wild-type and GnT-V Tg mice. These changes are similar to those seen in cancer progression but are unexpected in some aspects. In this review, we summarize what is known about GnT-V functions in skin and liver cells as a means to understand the physiological roles of GnT-V in mice.

• 한국정밀공학회지
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• 제26권4호
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• pp.120-127
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• 2009

Spindle units of machine tool are very important part in the manufacturing area. Recently high speed machining has become the main issue of metal cutting. To develop high speed machine tools, a lot of studies have been carried out for high speed spindle. Due to increase of the rotational speed of the spindle, there has been renewal of interest in vibration of spindle. This paper concerns the improvement of spindle design using design of experiments. To improve the design of critical speed and weight of spindle, the experiments using central composite method have been carried out. The targets are critical speed and weight of spindle. For optimization of critical speed and weight and optimization of only critical speed by operation of all area search through response optimizer, the result of analysis has improved design of each factor. Finite element analyses are performed by using the commercial codes ARMD, CATIA V5 and ANSYS workbench. From the results, it has been shown that the proposed method is effective for modification of spindle design to improve critical speed and weight.

• Steel and Composite Structures
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• 제37권5호
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• pp.571-587
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• 2020

In this article the seismic demand and performance of two recent braced steel frames named steel moment frames with the elliptic bracing (ELBRFs) are assessed through a laboratory program and numerical analyses of FEM. Here, one of the specimens is without connecting bracket from the corner of the frame to the elliptic brace (ELBRF-E), while the other is with the connecting brackets (ELBRF-B). In both the elliptic braced moment resisting frames (ELBRFs), in addition to not having any opening space problem in the bracing systems when installed in the surrounding frames, they improve structure's behavior. The experimental test is run on ½ scale single-story single-bay ELBRF specimens under cyclic quasi-static loading and compared with X-bracing and SMRF systems in one story base model. This system is of appropriate stiffness and a high ductility, with an increased response modification factor. Moreover, its energy dissipation is high. In the ELBRF bracing systems, there exists a great interval between relative deformation at the yield point and maximum relative deformation after entering the plastic region. In other words, the distance from the first plastic hinge to the collapse of the structure is fairly large. The experimental outcomes here, are in good agreement with the theoretical predictions.

• Wind and Structures
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• 제21권5호
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• pp.537-564
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• 2015

Excessive motions in buildings cause occupants to become uncomfortable and nervous. This is particularly detrimental to the tenants and ultimately the owner of the building, with respect to financial considerations. Serviceability issues, such as excessive accelerations and inter-story drifts, are more prevalent today due to advancements in the structural systems, strength of materials, and design practices. These factors allow buildings to be taller, lighter, and more flexible, thereby exacerbating the impact of dynamic responses. There is a growing need for innovative and effective techniques to reduce the serviceability responses of these tall buildings. The current study considers a case study of a real building to show the effectiveness and robustness of the TLD in reducing the coupled lateral-torsional motion of this high-rise building under wind loading. Three unique multi-modal TLD systems are designed specifically to mitigate the torsional response of the building. A procedure is developed to analyze a structure-TLD system using High Frequency Force Balance (HFFB) test data from the Boundary Layer Wind Tunnel Laboratory (BLWTL) at the University of Western Ontario. The effectiveness of the unique TLD systems is investigated. In addition, a parametric study is conducted to determine the robustness of the systems in reducing the serviceability responses. Three practical parameters are varied to investigate the robustness of the TLD system: the height of water inside the tanks, the amplitude modification factor, and the structural modal frequencies.

• Wind and Structures
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• 제18권1호
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• pp.83-101
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• 2014

Lamps installed on stay cables of cable-stayed bridges may alter the configuration of circular cross section of the cables and therefore result in aerodynamically unstable cable vibrations. The background of this study is a preliminary design of lamp installation on the cable-stayed He-dong Bridge in Guangzhou, China. Force measurements and dynamic response measurements wind tunnel tests were carried out to validate the possibility of cable galloping vibrations. It is observed that galloping will occur and the critical wind velocity is far less than the design wind velocity at Guangzhou City stipulated in Chinese Code. Numerical simulations utilizing software ANSYS CFX were subsequently performed and almost the same results as the wind tunnel tests were obtained. Moreover, the pressure and velocity contours around cable-lamp model obtained from numerical simulations indicated that the upstream steel wire in the preliminary design is the key factor for the onset of the galloping vibrations. A modification for the preliminary design of lamp installation, which suggests to remove the two parallel steel wires, is proposed, and it effectiveness is validated in further wind tunnel tests.

• 한국항공운항학회지
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• 제30권4호
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• pp.132-144
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• 2022

In response to the recent surge in aviation demand, major airport and aviation authorities continue to make efforts to formulate arrival procedures that take into account efficient aircraft separation, noise and environmental issues related to carbon (CO₂) emissions. In order to ensure efficient traffic control and environmental issues, as a result, a new concept Trombone, Point Merge, etc. have been introduced and widely used. However, these new concept incisions are becoming a factor that hinders operational efficiency and stability due to the restricted domestic airspace such as military airspace and excessive constraints of altitude, speed, etc. which do not reflect the concept of continuous descent operation and eventually needs to be modified to make continuous descent operation as feasible as possible. We herewith analyze and propose the way of improving flight safety and efficiency in the arrival operation procedure by supplementary modification which consequently contribute to the aviation industry international competitiveness.

• 콘크리트학회논문집
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• 제24권5호
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• pp.517-524
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• 2012

이 논문에서는 푸쉬오버해석을 통해 보-기둥 접합부 비탄성 전단거동과 고차모드를 고려한 횡하중 수직분포 형태가 구조물 거동에 미치는 영향을 알아보기 위해 지반조건 $S_B$ 내진설계범주 C에 대해서 5층 철근콘크리트 보통모멘트골조를 KBC2009에 맞게 구조설계 하였다. 보 및 기둥 부재의 휨모멘트-곡률 관계는 섬유모델(fiber model)로 확인하였으며 보-기둥 접합부 모멘트-회전각 관계는 simple and unified joint shear behavior model과 보-기둥 접합부 모멘트 평형관계를 이용하여 확인하였다. 푸쉬오버해석 결과 보-기둥 접합부를 강체로 고려하는 경우 구조물의 강성도 및 강도가 과대평가 되었으나 반응수정계수는 접합부 비탄성거동과 관계없이 KBC2009 보통모멘트골조 계수를 만족하여 구조 설계 과정에서 보-기둥 접합부의 비탄성 전단거동을 고려하지 않아도 문제가 없을 것으로 판단된다.

• Molecules and Cells
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• 제41권2호
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• pp.127-133
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• 2018

Chromatin remodeling factors are involved in many cellular processes such as transcription, replication, and DNA damage response by regulating chromatin structure. As one of chromatin remodeling factors, remodeling and spacing factor 1 (RSF1) is recruited at double strand break (DSB) sites and regulates ataxia telangiectasia mutated (ATM) -dependent checkpoint pathway upon DNA damage for the efficient repair. RSF1 is overexpressed in a variety of cancers, but regulation of RSF1 levels remains largely unknown. Here, we showed that protein levels of RSF1 chromatin remodeler are temporally upregulated in response to different DNA damage agents without changing the RSF1 mRNA level. In the absence of SNF2h, a binding partner of RSF1, the RSF1 protein level was significantly diminished. Intriguingly, the level of RSF1-3SA mutant lacking ATM-mediated phosphorylation sites significantly increased, and upregulation of RSF1 levels under DNA damage was not observed in cells overexpressing ATM kinase. Furthermore, failure in the regulation of RSF1 level caused a significant reduction in DNA repair, whereas reconstitution of RSF1, but not of RSF1-3SA mutants, restored DSB repair. Our findings reveal that temporal regulation of RSF1 levels at its post-translational modification by SNF2h and ATM is essential for efficient DNA repair.