• Biomedical Science Letters
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• v.9 no.3
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• pp.111-121
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• 2003

Recombinant single chain Fv (scFv) antibodies offer many advantages over mouse monoclonal antibodies such as faster clearance from blood, improved tumor localization, reduced human anti-mouse antibody (HAMA) response, and the availability to manipulate the scFv through genetic approaches. The recombinant phage display was constructed using lym-l hybridoma cells as a source of genetic starting material. mRNA was isolated from the corresponding antibodies hybridoma cells. VH and VL cDNA were amplified with RT-PCR and linked with ScFv by linker DNA to form ScFv DNA, which then were inserted into phagemid pCANTAB5E. The phage of positive clones selected with tube containing raji lymphoma cell and infected by competent E. coli HB2151 to express soluble scFv. The scFv lym-l was secreted into the cytosol and culture supernatant and shown to be of expected size (approximately 32 kDa) by western blot. An active scFv lym-l could be produced in E. coli with soluble form and high yield from hybridoma cell line, using phage display system. Immunoreactivity indicated that scFv lym1 showed a potential biding affinity against the raji lymphoma cell as its parental antibody (intact lym-l Ab).

• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.175-181
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• 2012

Drought and high salinity stresses often imposes adverse effects on crop yield. MYB transcription factors have been shown to be an important regulator in defense responses to these environmental stresses. In this study, we have cloned and characterized a soybean gene GmMYB184 (Glycine max MYB transcription factor 184). Deduced amino acid sequences of GmMYB184 show highest homology with that from Vitis vinifera legume plant (75%). Different expression patterns of GmMYB184 mRNA were observed subjected to drought, cold, high salinity stress and abscisic acid treatment, suggesting its role in the signaling events in the osmotic stress-related defense response. Subcellular localization studies demonstrated that the GFP-GmMYB184 fusion protein was localized in the nucleus. Using the yeast assay system, the C-terminal region of GmMYB184 was found to be essential for the transactivation activity. These results indicate that the GmMYB184 may play a role in abiotic stress tolerance in plant.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2014.10a
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• pp.110-115
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• 2014

The nuclear power plant was launched by Kori unit 1 in 1978 years. Currently, 23 nuclear power plants have been operating in Korea since 1978 years. The localization was completed for most of the reactor facility from Hanbit(Youngkwang) unit 3&4. However, RCP(Reactor Coolant Pump) and MMIS(Man Machine Interface System) is an important technology that has been excluded from the scope of the technical transfer has been dependent on a specific overseas vendor. Recent success in RCP development through co-operation with government and industries. Developed RCP will be applied to Shin-Hanul unit 1&2 nuclear power plants. The RCP operates in high speed and high pressure condition and only rotating component in the NSSS(Nuclear Steam Supply System). Therefore, the problem of vibration has arisen caused by the hydraulic forces of the working fluid. These forces can influence on the stability characteristics for entire RCS(Reactor Coolant System) loop, and can act as significant destabilizing forces. In this study, vibration evaluation of the pump shaft of development RCP estimated under normal operation and over speed conditions. In order to predict the vibration characteristics and dynamic behavior, modal analysis, critical speed analysis and unbalance response spectrum analysis were performed.

• Molecules and Cells
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• v.22 no.1
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• pp.58-64
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• 2006

WRKY family proteins are a class of plant-specific transcription factors involved in stress response signaling pathways. In this study a gene encoding a putative WRKY protein was isolated from a pepper EST database (http://genepool.kribb.re.kr). The cDNA, named Capsicum annuum WRKY2 (CaWRKY2), encodes a putative polypeptide of 548 amino acids, containing two WRKY domains with zinc finger motifs and two potential nuclear localization signals. Northern blot analyses showed that CaWRKY2 mRNA was preferentially induced during incompatible interactions of pepper plants with PMMoV, Pseudomonas syringae pv. syringae 61, and Xanthomonas axonopodis pv. vesicatoria race 3. Furthermore, CaWRKY2 transcripts were strongly induced by wounding and ethephon treatment, whereas only moderate expression was detected following treatment with salicylic acid and jasmonic acid. CaWRKY2 was translocated to the nucleus when a CaWRKY2-smGFP fusion construct was expressed in onion epidermal cells. CaWRKY2 also had transcriptional activation activity in yeast. Taken together our data suggest that CaWRKY2 is a pathogen-inducible transcription factor that may have a role in early defense responses to biotic and abiotic stresses.

• Molecules and Cells
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• v.19 no.3
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• pp.334-341
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• 2005

Plants are sessile and rely on a wide variety of growth hormones to adjust growth and development in response to internal and external stimuli. We have identified a gene, designated NAN, encoding a basic helix-loop-helix (bHLH) transcription factor that regulates cell elongation and seed germination in plants. NAN has an HLH motif in its C-terminal region but does not have any other discernible homologies to bHLH proteins. A bipartite nuclear localization signal is located close to the HLH motif. An Arabidopsis mutant, nan-1D, in which NAN is activated by the insertion of the 35S enhancer, exhibits growth retardation with short hypocotyls and curled leaves. It is also characterized by reduced seed germination and apical hook formation, symptomatic of GA deficiency or disrupted GA signaling. The phenotypic effects of nan-1D were increased by treatment with paclobutrazol (PAC), an inhibitor of gibberellic acid (GA) biosynthesis. NAN is constitutively expressed throughout the life cycle. Our observations indicate that NAN has a housekeeping role in plant growth and development, particularly in seed germination and cell elongation, and that it may modulate GA signaling.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.6
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• pp.1302-1309
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• 2014

In this paper, we propose a method to improve the performance of the direction-of-arrival (DOA) estimation of a speech source using a multiple signal classification (MUSIC)-based algorithm. Basically, the proposed algorithm utilizes a complex coefficient band pass filter to generate the narrow band signals for signal analysis. Also, reverberation component reduction and quadratic function-based response approximation in MUSIC spatial spectrum are utilized to improve the accuracy of DOA estimation. Experimental results show that the proposed method outperforms the well-known generalized cross-correlation (GCC)-based DOA estimation algorithm in the aspect of the estimation error and success rate, respectively.Abstract should be placed here. These instructions give you guidelines for preparing papers for JICCE.

• BMB Reports
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• v.50 no.5
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• pp.257-262
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• 2017

The subcellular localization of Bax plays a crucial role during apoptosis. In response to apoptotic stimuli, Bax translocates from the cytoplasm to the mitochondria, where it promotes the release of cytochrome c to the cytoplasm. In cells infected with HSV-1, apoptosis is triggered or blocked by diverse mechanisms. In this study, we demonstrate how HSV-1 ICP27 induces apoptosis and modulates mitochondrial membrane potential in HEK 293T cells. We found that ICP27 interacts with $14-3-3{\theta}$ which sequesters Bax to the cytoplasm. In addition, ICP27 promotes the translocation of Bax to the mitochondria by inhibiting the interaction between $14-3-3{\theta}$ and Bax. Our findings may provide a novel apoptotic regulatory pathway induced by ICP27 during HSV-1 infection.

• Molecules and Cells
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• v.22 no.3
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• pp.262-268
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• 2006

During endometrial differentiation the extracellular matrix (ECM) changes dramatically to prepare for implantation of the embryo. However, the genes regulating the ECM build-up in the uterine endometrium during early pregnancy are not well known. Using the PCR-select cDNA subtraction method, dermatopontin was identified in the uterus of a pregnant mouse on day 4 of gestation. Dermatopontin mRNA increased dramatically on day 3, and was at its highest level at the time of implantation. Administration of RU 486 significantly inhibited mRNA expression by day 4 of gestation, but ICI 182,780 did not. Progesterone markedly induced dermatopontin expression in ovariectomized uteri within 4 h of administration, whereas estrogen had little effect. In silico analysis revealed progesterone receptor binding sites in the dermatopontin promoter region. Decidualization did not induce expression of dermatopontin; instead dermatopontin mRNA became strongly localized at the interimplantation site. In situ hybridization revealed that expression gradually decreased in the luminal epithelial cells as pregnancy progressed, whereas it increased in the stromal cells. The pattern of localization and the changes of intensity of dermatopontin mRNA coincided with those of collagen. Collectively, these results strongly suggest that dermatopontin expression is steroid-dependent. They also suggest that, at the time of implantation, dermatopontin expression is primarily regulated spatio-temporally by progesterone via progesterone receptors, and is modulated by the decidual response during implantation. Dermatopontin may be one of the regulators used to remodel the uterine ECM for pregnancy.

• Journal of the Korea Institute of Military Science and Technology
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• v.5 no.2
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• pp.169-184
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• 2002

In order to extract bearing information from the directional sensors of DIFAR(directional frequency analysis and recording) that is a kind of passive sonobuoy, the cardioid beamforming algorithm applicable to DIFAR system was studied in the frequency domain. the algorithm uses narrow-band signals propagated though the media from the acoustic sources such as ship machineries. The proposed algorithm is expected to give signal to noise ratio of 6dB when it uses the maximum response axis(MRA) among the Cardioid beams. The estimated bearings agree very well with those from GPS data. Assuming the bearings from GPS data to be real values, the estimation errors are analyzed statistically. The histogram of estimation errors in each frequency have Gaussian shape, the mean and standard deviation dropping in the ranges -1.1~$6.7^{\circ}$ and 13.3~$43.6^{\circ}$, respectively. Estimation errors are caused by SMR degradation due to propagation loss between the source and receiver, daily fluctuating geo-magnetic fields, and non-stationary background noises. If multiple DIFAR systems are employed, in addition to bearing, range information could be estimated and finally localization or tracking of a target is possible.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.32 no.5
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• pp.14-25
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• 2000

In this investigation, a wavelet transform analysis was used to decompose beta-radiographic formation images into spectral and spatial components. Conventional formation analysis may use spectral analysis, based on Fourier transformation or variance vs. zone size, to describe the grammage distribution of features such as flocs, streaks and mean fiber orientation. However, these methods have limited utility for the analysis of statistically stationary data sets where variance is not uniform with position, e.g. paper machine CD profiles (especially those that contain streaks). A continuous wavelet transform was used to analyze formation data arrays obtained from radiographic imaging of handsheets and cross machine paper samples. The response of the analytical method to grammage, floc size distribution, mean fiber orientation an sensitivity to feature localization were assessed. From wavelet analysis, the change in scale of grammage variation as a function of position was used to demonstrate regular and isolated differences in the formed structure.