• IMMUNE NETWORK
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• 제12권3호
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• pp.118-125
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• 2012

CD40-CD40L-mediated help from CD4 T cells is essential to induce primary CD8 T cell responses specific to the non-inflammatory cell-based antigen H60. In this study, using H60 as a model antigen, we generated recombinant vaccinia viruses (rVVs) expressing the H60 CD8 epitope and investigated whether CD4 help was required to activate the CD8 T cell response specific to the virally expressed H60. The immune response after infection with rVVs expressing H60 was similar to that after immunization with H60 congenic splenocytes, with a peak frequency of H60-specific CD8 T cells detected in the blood on day 10 post-infection. A CD8 T cell response specific for virally derived H60 was not induced in CD4-depleted mice, but was in CD40-deficient mice. These results provide insights into the characterization of the CD8 T cell response specifically for antigens originating from cellular sources compared to viral sources.

• Advances in Computational Design
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• 제8권2호
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• pp.97-112
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• 2023

The Finite Element (FE) modeling of Reinforced Concrete (RC) under seismic loading has a sensitive impact in terms of getting good contribution compared to experimental results. Several idealized model types for simulating the nonlinear response have been developed based on the plasticity distribution alone the model. The Continuum Models are the most used category of modeling, to understand the seismic behavior of structural elements in terms of their components, cracking patterns, hysteretic response, and failure mechanisms. However, the material modeling, contact and nonlinear analysis strategy are highly complex due to the joint operation of concrete and steel. This paper presents a numerical simulation of a chosen RC column under monotonic and cyclic loading using the FE Abaqus, to assessthe hysteretic response and failure mechanisms in the RC columns, where the perfect bonding option is used for the contact between concrete and steel. While results of the numerical study under cyclic loading compared to experimental tests might be unsuccessful due to the lack of bond-slip modeling. The monotonic loading shows a good estimation of the envelope response and deformation components. In addition, this work further demonstrates the advantage and efficiency of the damage distributions since the obtained damage distributions fit the expected results.

• Advances in aircraft and spacecraft science
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• 제5권2호
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• pp.187-204
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• 2018

The sine sweep base excitation test campaign is a major milestone in the process of mechanical qualification of space structures. The objectives of these vibration tests are to qualify the specimen with respect to the dynamic environment induced by the launcher and to demonstrate that the spacecraft FE model is sufficiently well correlated with the test specimen. Dynamic qualification constraints lead to performing base excitation sine tests using a sine sweep over a prescribed frequency range such that at each frequency the response levels at all accelerometers, load cells and strain gages is the same as the steady state response. However, in practice steady state conditions are not always satisfied. If the sweep rate is too high the response levels will be affected by the presence of transients which in turn will have a direct effect on the estimation of modal parameters. A study funded by ESA and AIRBUS D&S was recently carried out in order to investigate the influence of sine sweep rates in actual test conditions. This paper presents the results of this study along with recommendations concerning the choice of methods.

• 센서학회지
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• 제21권6호
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• pp.425-428
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• 2012

This paper presents the fabrication and characterization of graphene based hydrogen sensors. Graphene was synthesized by annealing process of Ni/3C-SiC thin films. Graphene was transferred onto oxidized Si substrates for fabrication of chemiresistive type hydrogen sensors. Au electrode on the graphene shows ohmic contact and the resistance is changed with hydrogen concentration. Nanoparticle catalysts of Pd and Pt were decorated. Response factor and response (recovery) time of hydrogen sensors based on the graphene are improved with catalysts. The response factors of pure graphene, Pt and Pd doped graphenes are 0.28, 0.6 and 1.26, respectively, at 50 ppm hydrogen concentration.

• Journal of Information Display
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• 제11권2호
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• pp.57-62
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• 2010

A method of characterizing time-sequential stereoscopic 3D displays based on the measurement of the temporal behavior of the systems vs. the grey levels is proposed. An Nvidia 3D vision kit with a 3D-ready SAMSUNG 2233RZ LCD display is characterized in the paper. OPTISCOPE SA especially designed for the precise measurements of the luminance and temporal behavior of LCD displays was used. The transmittance and response time of the shutter glasses was first evaluated. Then the grey-to-grey response times of the display were measured. The 2D and 3D behaviors of the display were then compared. Finally, the temporal behavior of the complete system was modeled, and the grey-level variations on one view were deduced as a function of the synchronization and level of the other eye. The main sources of imperfection were identified and quantified, and a full computation of the system performances was done.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2005년도 추계종합학술대회
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• pp.891-894
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• 2005

SU-8은 부드러운 벽면을 가지는 두꺼운 패턴을 제작하는 데 사용되는 음성 감광제(negative photoresist)이다 .이것은 처리 후에 강성이 높고 화학적으로 강인한 장점을 가지고 있으며 최근 MEMS 디바이스의 구조체로 쓰이고 있다. 그러나 SU-8은 공정 처리요소들에 대하여 매우 민감하고 사용하기 어려운 것으로 알려져 있다. 본 연구에서는 공정 처리요소로 exposure energy, post exposure bake (PEB) temperature, PEB time을 조절하여 실험을 하였다. Response Surface Methodology (RSM)를 이용해 각 인자가 delamination에 미치는 영향에 대해 분석하였고 이를 바탕으로 SU-8의 delamination을 최소화하기 위한 처리요소들의 최적화 방안을 제시하였다.

• Journal of Plant Biotechnology
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• 제4권2호
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• pp.53-58
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• 2002

Adverse environmental conditions such as drought, high salt and cold/freezing are major factors that reduces crop productivity worldwide. According to a survey, 50-80% of the maximum potential yield is lost by these "environmental or abiotic stresses", which is approximately ten times higher than the loss by biotic stresses. Thus, improving stress-tolerance of crop plants is an important way to improve agricultural productivity, In order to develop such stress-tolerant crop plants, we set out to identify key stress signaling components that can be used to develop commercially viable crop varieties with enhanced stress tolerance. Our primary focus so far has been on the identification of transcription factors that regulate stress responsive gene expression, especially those involved in ABA-mediated stress response. Be sessile, plants have the unique capability to adapt themselves to the abiotic stresses. This adaptive capability is largely dependent on the plant hormone abscisic acid (ABA), whose level increases under various stress conditions, triggering adaptive response. Central to the response is ABA-regulated gene expression, which ultimately leads to physiological changes at the whole plant level. Thus, once identified, it would be possible to enhance stress tolerance of crop plants by manipulating the expression of the factors that mediate ABA-dependent stress response. Here, we present our work on the isolation and functional characterization of the transcription factors.n factors.

• Structural Engineering and Mechanics
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• 제58권5호
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• pp.887-903
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• 2016

This study analyses the seismic response of a three-dimensional (3-D) rigid massless square foundation resting or embedded in a viscoelastic soil limited by rigid bedrock. The foundation is subjected to harmonic oblique seismic waves P, SV, SH and R. The key step is the characterization of the soil-foundation interaction by computing the impedance matrix and the input motion matrix. A 3-D frequency boundary element method (BEM) in conjunction with the thin layer method (TLM) is adapted for the seismic analysis of the foundation. The dynamic response of the rigid foundation is solved from the wave equations by taking into account the soil-foundation interaction. The solution is formulated using the frequency BEM with the Green's function obtained from the TLM. This approach has been applied to analyze the effect of soilstructure interaction on the seismic response of the foundation as a function of the kind of incident waves, the angles of incident waves, the wave's frequencies and the embedding of foundation. The parametric results show that the non-vertical incident waves, the embedment of foundation, and the wave's frequencies have important impact on the dynamic response of rigid foundations.

• 한국해양생명과학회지
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• 제8권2호
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• pp.186-189
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• 2023

Uncoupling protein 1 (UCP1) is a unique mitochondrial membranous protein expressed in brown adipose tissue (BAT) in mammals. While its expression in response to cold temperatures and adipogenic inducers is well-characterized in mammals and human infants, the molecular characterization and expression of UCP1 in fish remain unexplored. To address this gap, we analyzed UCP1 expression in response to adipogenic inducers in a fish cell line, rainbow trout gonadal cells (RTG-2), and compared it with UCP1 expression in three mammalian preadipocytes, 3T3-L1, T37i, and WT1 exposed to the Peroxisome proliferator-activated receptor gamma (PPARγ) agonists, rosiglitazone (Rosi). In mammalian preadipocytes, UCP1 protein was highly expressed by Rosi, with an induction of adipogenesis observed in a time-dependent manner. This suggests that UCP1 plays a significant role in adipogenesis in mammals. However, RTG-2 cells showed no response to adipogenic inducers and exhibited only marginal expressions of UCP1. These results imply that RTG-2 cells may lack crucial responsive mechanisms to adipogenic signals or that the adipogenic response is regulated by other mechanisms. Further studies are needed to confirm these phenomena in fish preadipocytes when an appropriate cell line is established in future research.

• Carbon letters
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• 제17권1호
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• pp.39-44
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• 2016

The work presented in this report was a detailed comparative study of the electrochemical response exhibited by graphite anodes in Li-ion batteries having different physical features. A comprehensive morphological and physical characterization was carried out for these graphite samples via X-ray diffraction and scanning electron microscopy. Later, the electrochemical performance was analyzed using galvanostatic charge/discharge testing and the galvanostatic intermittent titration technique for these graphite samples as negative electrode materials in battery operation. The results demonstrated that a material having a higher crystalline order exhibits enhanced electrochemical properties when evaluated in terms of rate-capability performance. All these materials were investigated at high C-rates ranging from 0.1C up to 10C. Such improved response was attributed to the crystalline morphology providing short layers, which facilitate rapid Li⁺ ions diffusivity and electron transport during the course of battery operation. The values obtained for the electrical conductivity of these graphite anodes support this possible explanation.