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http://dx.doi.org/10.4110/in.2012.12.3.118

Requirement of CD4 Help for Induction of CD8 T Cell Response Specific for Virally Derived H60  

Ryu, Su-Jeong (Department of Biomedical Sciences, Seoul National University College of Medicine)
Kang, Bo-Ra (Department of Biomedical Sciences, Seoul National University College of Medicine)
Kim, Seok-Ho (Therapeutic Antibody Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Tae-Woo (Graduate School of Medicine, Korea University)
Chang, Jun (Division of Life and Pharmaceutical Sciences, Ewha Woman's University)
Choi, Eun-Young (Department of Biomedical Sciences, Seoul National University College of Medicine)
Publication Information
IMMUNE NETWORK / v.12, no.3, 2012 , pp. 118-125 More about this Journal
Abstract
CD40-CD40L-mediated help from CD4 T cells is essential to induce primary CD8 T cell responses specific to the non-inflammatory cell-based antigen H60. In this study, using H60 as a model antigen, we generated recombinant vaccinia viruses (rVVs) expressing the H60 CD8 epitope and investigated whether CD4 help was required to activate the CD8 T cell response specific to the virally expressed H60. The immune response after infection with rVVs expressing H60 was similar to that after immunization with H60 congenic splenocytes, with a peak frequency of H60-specific CD8 T cells detected in the blood on day 10 post-infection. A CD8 T cell response specific for virally derived H60 was not induced in CD4-depleted mice, but was in CD40-deficient mice. These results provide insights into the characterization of the CD8 T cell response specifically for antigens originating from cellular sources compared to viral sources.
Keywords
Recombinant vaccinia virus; Viral epitope; CD4 help; CD8 T cell response;
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