• Title/Summary/Keyword: Respirometer

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Microbial Activity of Gravel Intertidal Zone for Purification of Polluted Near Shore Water

  • Song, Young-Chae;Gu, Ja-Hwan;Park, In-Seok;Yoo, Jong-Su
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2004.04a
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    • pp.233-239
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    • 2004
  • Microbial activity of biofilm formed on the surface of gravels from intertidal zone was estimated using an aerobic respirometer system, and compared with that of suspended marine microorganisms contained in a near shore water. The maximum oxygen uptake rate of the suspended marine microorganisms was 0.15mg O$_2$/L/hr, indicating the potential of purification of polluted near shore water. For the gravels from the intertidal zone, the maximum uptake rate of oxygen was affected by the vertical positions, but their gross value was 0.77mg O$_2$/L/hr, which was around 5.1 times higher than the purification potential of polluted near shore water by the microorganisms contained in the near shore water. The nitrogen removed by the gravels from the intertidal zone and the marine microorganisms was about 1/20-1/39 times of the total consumption of oxygen, which was similar to that of the phosphate. The gravel intertidal zone contained lots of particulate organics, over than that in the near shore water, and this was confirmed from the large difference between total oxygen consumption and the removed soluble COD in the microbial activity test. This indicates that the gravel intertidal zone plays an important role in controlling the non-point source pollutants from land, as well as self-purification of polluted near shore water by trapping and degrading the particulate organics.

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Effect of Water Temperature and Photoperiod on the Oxygen Consumption Rate of Juvenile Pacific Cod Gadus macrocephalus (대구 Gadus macrocephalus 치어의 산소 소비율에 미치는 수온과 광주기의 영향)

  • Oh, Sung-Yong;Park, Heung-Sik;Kim, Chong-Kwan
    • Ocean and Polar Research
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    • v.32 no.3
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    • pp.229-236
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    • 2010
  • A study was conducted to investigate the effect of water temperature and photoperiod on the oxygen consumption of the fasting juvenile Pacific cod Gadus macrocephalus (mean body weight 79.9${\pm}$2.0 g) in order to quantify metabolic response of the species under given conditions. The oxygen consumption rate (OCR) of G. macrocephalus was measured under a combination of four different water temperatures (7, 10, 13 and $16^{\circ}C$) and three different photoperiods (24L:0D, 12L:12D and 0L:24D) with an interval of 5 minutes over a 24-hour period using a closed recirculating respirometer. Three replicates were set up in each treatment. OCRs increased with increased water temperatures under all photoperiod conditions (P<0.001). Mean OCRs at 7, 10, 13 and 16oC ranged from 793.7~1108.4, 1145.7~1570.3, 1352.8~1742.5 and 1458.2~1818.6 mg $O_2$ $kg^{-1}$ $h^{-1}$, respectively. Under all water temperature conditions except $7^{\circ}C$ (P<0.001), mean OCRs of G. macrocephalus were the highest in continuous light (24L:0D) followed by 12L:12D and 0L:24D photoperiods. Mean OCRs of fish exposed to the 12L:12D photoperiod were significantly higher during the light phase than during the dark phase under all temperature conditions (P<0.001). $Q_{10}$ values ranged from 3.19~5.13 between 7 and $10^{\circ}C$, 1.41~1.74 between 10 and $13^{\circ}C$ and 1.15~1.35 between 13 and $16^{\circ}C$, respectively. Based on overall results, water temperature, photoperiod and their combinations exerted a significant influence on the metabolic rate of juvenile cod. This study provides empirical data for estimating the amount of oxygen demand and managing the culture of cod under the given water temperatures and photoperiods.

The Effect of Salt in Medicine for Salt Pharmacopuncture (염약침(鹽藥鍼)을 위한 소금의 의학적 효용)

  • Kim, Kee Hyun;Kim, Jee Hwa;Song, Ho Sueb
    • Journal of Acupuncture Research
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    • v.32 no.2
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    • pp.131-145
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    • 2015
  • Objectives : The purpose of this study was to demonstrate the effectiveness and availability of salt pharmacopuncture of Korean sun-dried salt and processing salt on the basis of bibliographical review. Methods : This study was done through a narrative review of related bibliography including medical books, journals, general bibliography, and web sites. Results : 1. Korean sun-dried salt is produced in the ground with soil, tiles, and pottery. Sun-dried, traditional, granulated, bamboo, and grilled salts are all appropriate for medical use. They are composed of a lower level of NaCl and unnecessary substances, and have a higher water and mineral content. 2. The 9~30 g of NaCl included in the physiological saline matches the ratio of a normal human body. However, if the saline is used for water supply, it should be improved because the recommended amount is much lower. 3. Medicine that includes NaCl is applied for lessening the congestion of the nasal cavity, alleviating cornea swelling, or for a lack of water. NaCl can also be used for washing contact lenses as well as cleaning the mucous membrane of the eyes and nose. 4. Salt relieves anger, detoxifies, induces vomiting, builds strong muscles and bones, gives energy, slows aging, and ultimately improves health. 5. Salt treats many diseases including: cardiovascular, hematosis, respirometer, obstetric, musculoskeletal, mineral supplement, eye, teeth and skin issues, etc.. However, salt is not used for treating asthma, cough, or other ailments mainly affecting the vascular system. 6. While NaCl in salt absorbs water and the vascular constriction results in higher blood pressure, the pressure induced from salt is actually a physiologically temporary rise. Rather, salt helps remove oil from the body and its potassium lowers blood pressure. Conclusions : It was suggested that salt pharmacopuncture of Korean sun-dried salt and processing salt should be made available for adjusting the physiological salt concentration and control of the Na side effects, therefore it would be useful in the treatment of diseases.

Microbial Activity of Gravel Intertidal Zone for Purification of Polluted Near Shore Water

  • Song, Young-Chae;Gu, Ja-Hwan;Park, In-Seok;Yoo, Jong-Su
    • Journal of Navigation and Port Research
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    • v.28 no.2
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    • pp.155-159
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    • 2004
  • Microbial activity of biofilm formed on the surface of gravels from intertidal zone was estimated using an aerobic respirometer system, and compared with that of suspended marine microorganisms contained in a near shore water, The maximum oxygen uptake rate of the suspended marine microorganisms was 0.15mg$O_2$/L/hr, indicating the potential of purification of polluted near shore water. For the gravels from the intertidal zone, the maximum uptake rate of oxygen was affected by the vertical positions, but their gross value was 0.77mg $O_2$/L/hr, which was around 5.1 times higher than the purification potential of polluted near shore water by the microorganisms contained in the near shore water. The nitrogen removed by the gravels from the intertidal zone and the marine microorganisms was about 1/20-1/39 times of the total consumption of oxygen, which was similar to that of the phosphate. The gravel intertidal zone contained lots of particulate organics, over than that in the near shore water, and this was confirmed from the large difference between total oxygen consumption and the removed soluble COD in the microbial activity test. This indicates that the gravel intertidal zone plays an important role in controlling the non-point source pollutants from land, as well as self-purification of polluted near shore water by trapping and degrading the particulate organics.

Oxygen Consumption of Sea Squirt Halocynthia roretzi Depending on the Water Temperature and Body Size (수온과 크기에 따른 멍게(Halocynthia roretzi)의 산소 소비)

  • Kang, Pil Jun;Lee, Geun Su;Oh, Sung-Yong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.4
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    • pp.449-454
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    • 2022
  • The oxygen consumption rate (OCR) based on the water temperature and body size of the sea squirt Halocynthia roretzi was examined to provide quantitative information about the metabolic response of the species. OCRs were measured using a closed flow-through respirometer at four different water temperatures (10, 15, 20 and 25℃) and two different body sizes (21.4±1.1 g and 150.5±1.3 g, wet weight) with triplicates of each treatment. OCR increased as water temperature increased at both body sizes, but decreased as body size increased regardless of the water temperature (P<0.001). The effect of body size evaluated as a power function ranged from 0.8055 to 0.8884. The highest Q10 values in the small and large size groups ranged from 15 to 20℃ and 20 to 25℃, respectively. The metabolic daily energy loss rate via respiration at all tested temperatures ranged from 56.2 to 106.1 J g-1 d-1 in the small-size group and from 44.5 to 92.0 J g-1 d-1 in the large-size group. Our results indicate that the metabolic response of H. roretzi highly depends on fluctuating water temperature at a given life stage.

External Attachment of Pop-up Satellite Archival Tag (PSAT) and Water Temperature Affect Oxygen Consumption Rate of the Olive Flounder Paralichthys olivaceus (넙치(Paralichthys olivaceus) 산소 소비율에 미치는 Pop-up Satellite Archival Tag (PSAT) 체외 부착과 수온의 영향)

  • Geun Su Lee;Pil Jun Kang;Hye Mi Park;Sung-Yong Oh
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.5
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    • pp.660-666
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    • 2023
  • This study aimed to examine the effect of external pop-up satellite archival tags (PSATs) attachment and water temperature on the oxygen consumption rate (OCR) of the olive flounder (mean body weight 2281.7 g). The OCRs of fish were measured under conditions of three different water temperature conditions (15, 20, and 25℃) and two different tagging methods [non-tagging, control; bio-logger external attachment with a miniature PSAT (dummy mrPAT), BEA] using a closed flow-through respirometer. The OCRs of fish linearly increased with the increase in water temperature in both the control and BEA (P<0.001); however, the OCRs of BEA were approximately 1.8-1.9 times lower than those of the control at each water temperature (P<0.001). The Q10 values of the control and BEA were the highest in the water temperature range of 15 to 20℃, but sensitivity to water temperature changes was higher in BEA than in the control. The metabolic energy loss rate (MEL) of fish increased with increasing water temperature regardless of external tagging, but the MEL of the control was higher than that of BEA (P<0.001). These results demonstrate that OCR, thermal sensitivity, and energy expenditure are all affected in adult olive flounder with external PSAT attachment.

A Study of Organic Matter Fraction Method of the Wastewater by using Respirometry and Measurements of VFAs on the Filtered Wastewater and the Non-Filtered Wastewater (여과한 하수와 하수원액의 VFAs 측정과 미생물 호흡률 측정법을 이용한 하수의 유기물 분액 방법에 관한 연구)

  • Kang, Seong-wook;Cho, Wook-sang
    • Journal of the Korea Organic Resources Recycling Association
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    • v.17 no.1
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    • pp.58-72
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    • 2009
  • In this study, the organic matter and biomass was characterized by using respirometry based on ASM No.2d (Activated Sludge Model No.2d). The activated sludge models are based on the ASM No.2d model, published by the IAWQ(International Association on Water Quality) task group on mathematical modeling for design and operation of biological wastewater treatment processes. For this study, OUR(Oxygen Uptake Rate) measurements were made on filtered as well as non-filtered wastewater. Also, GC-FID and LC analysis were applied for the estimation of VFAs(Volatile Fatty Acids) COD(S_A) in slowly bio-degradable soluble substrates of the ASM No.2d. Therefore, this study was intended to clearly identify slowly bio-degradable dissolved materials(S_S) and particulate materials(X_I). In addition, a method capable of determining the accurate time to measure non-biodegradable COD(S_I), by the change of transition graphs in the process of measuring microbial OUR, was presented in this study. Influent fractionation is a critical step in the model calibrations. From the results of respirometry on filtered wastewater, the fraction of fermentable and readily biodegradable organic matter(S_F), fermentation products(S_A), inert soluble matter(S_I), slowly biodegradable matter(X_S) and inert particular matter(X_I) was 33.2%, 14.1%, 6.9%, 34.7%, 5.8%, respectively. The active heterotrophic biomass fraction(X_H) was about 5.3%.

Optimal DO Setpoint Decision and Electric Cost Saving in Aerobic Reactor Using Respirometer and Air Blower Control (호흡률 및 송풍기 제어 기반 포기조 최적 DO 농도 설정과 전력 비용 절감 연구)

  • Lee, Kwang Su;Kim, Minhan;Kim, Jongrack;Yoo, Changkyoo
    • Korean Chemical Engineering Research
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    • v.52 no.5
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    • pp.581-586
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    • 2014
  • Main objects for wastewater treatment operation are to maintain effluent water quality and minimize operation cost. However, the optimal operation is difficult because of the change of influent flow rate and concentrations, the nonlinear dynamics of microbiology growth rate and other environmental factors. Therefore, many wastewater treatment plants are operated for much more redundant oxygen or chemical dosing than the necessary. In this study, the optimal control scheme for dissolved oxygen (DO) is suggested to prevent over-aeration and the reduction of the electric cost in plant operation while maintaining the dissolved oxygen (DO) concentration for the metabolism of microorganisms in oxic reactor. The oxygen uptake rate (OUR) is real-time measured for the identification of influent characterization and the identification of microorganisms' oxygen requirement in oxic reactor. Optimal DO set-point needed for the micro-organism is suggested based on real-time measurement of oxygen uptake of micro-organism and the control of air blower. Therefore, both stable effluent quality and minimization of electric cost are satisfied with a suggested optimal set-point decision system by providing the necessary oxygen supply requirement to the micro-organisms coping with the variations of influent loading.

Prediction of Chemical Organic Composition of Manure by Near Infrared Reflectance Spectroscopy

  • Amari, Masahiro;Fukumoto, Yasuyuki;Takada, Ryozo
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1265-1265
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    • 2001
  • The organic materials included in excreta of livestock are important resources for organic manure and for improving soil quality, although there is still far from effective using. One reason for this is still unclearly standard of quality for evaluation of manure made from excreta of livestock. Therefore, the objective of this study is to develop rapid and accurate analytical method for analyzing organic compositions of manure made from excreta of livestock, and to establish quality evaluation method based on the compositions predicted by near infrared reflectance spectroscopy (NIRS). Sixteen samples of manure, each eight samples prepared from two treatments, were used in this study. The manure samples were prepared by mixing 560 kg feces of swine,60 kg sawdust with moisture content was adjusted to be 65%. The mixture was then keep under two kinds of shelter, black and clear sheets, as a treatment on the effect of sunlight. Samples were taken in every week (form week-0 to 7) during the process of manure making. Samples were analyzed to determine neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) by detergent methods, and organic cell wall (OCW) and fibrous content of low digestibility in OCW (Ob) by enzymatic methods. Biological oxygen demand (BOD) was analyzed by coulometric respirometer method. These compositions were carbohydrateds and lignin that were hardly digested. Spectra of samples were scanned by NIR instrument model 6500 (Pacific Scientific) and read over the range of wavelength between 400 and 2500nm. Calibration equations were developed using eight manure samples collected from black sheet shelter, while prediction was conducted to the other eight samples from clear sheet shelter. Accuracy of NTRS prediction was evaluated by correlation coefficients (r), standard error of prediction (SEP) and ration of standard deviation of reference data in prediction sample set to SEP (RPD). The r, SEP and RPD value of forage were 0.99, 0.69 and 7.6 for ADL, 0.96, 1.03 and 4.1 for NDF, 0.98, 0.60 and 4.9 for ADF, 0.92, 1.24 and 2.6 for Ob, and 0.91, 1.02 and 7.3 for BOD, respectively. The results indicated that NIRS could be used to measure the organic composition of forage used in manure samples.

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Effects of Water Temperature Changes on the Oxygen Consumption Rhythm in the Japanese eel, Anguilla japonica

  • Kim, Jong-Wook;Lee, Tae-Won;Noh, Il;Kim, Wan-Soo
    • Journal of Environmental Science International
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    • v.20 no.8
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    • pp.943-951
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    • 2011
  • We investigated the effects of temperature changes on the oxygen consumption rhythm in Japanese eels, Anguilla japonica, using an automatic intermittent flow respirometer (AIFR). The endogenous rhythm of the oxygen consumption rate (OCR) in the eels (n = 18; 44-74 cm, 145-690 g), freshly collected by bag net from estuaries, was nearly synchronous with the tidal pattern of the estuarine collection site. The magnitude of mean OCR (mOCR) of eels showed variable range of 82.2 - 116.5 ml $O_2\;kg^{-1}ww \;h^{-1}$ under constant conditions. In case of increasing temperature from 25 to $38^{\circ}C$, the OCR of eels exhibited a gradually increasing trend with a rhythmic pattern until $36^{\circ}C$. Above $36^{\circ}C$ the rhythms of the OCR dampened and the OCR decreased rapidly at around $36-37^{\circ}C$. The OCR of the eels exhibited the maximum value at $38^{\circ}C$, and then it sharply decreased. The results suggested that the critical thermal maximum (CTM) regarding the endogenous rhythms of the eels was at around $36-37^{\circ}C$ when water temperature increased at $0.5^{\circ}C$/14 h following the acclimation at $25^{\circ}C$. In case of decreasing temperature ($0.5^{\circ}C$/14 h) from 25 to $0^{\circ}C$, the OCR of the eels displayed a abrupt decrease up to $23^{\circ}C$, and between at 23 and $20^{\circ}C$, there was an agitation which showed a slight increase in the OCR with a duration of 1-2 days. Below $9^{\circ}C$, the OCR rhythm of the eels showed a constant state regardless of temperature decreasing. These results suggest that the Japanese eel has an upper incipient lethal temperature at $36^{\circ}C$, with a lower thermal limit at $9^{\circ}C$. The biochemical aspects of the eels influenced by water temperature need to be further studied.