• 한국식품위생안전성학회지
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• 제32권3호
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• pp.243-248
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• 2017

현재 식품공전에 식품원료로 사용이 가능한 것으로 등록된 국내 산림지역 자생 식물을 식품산업에 활용하고자 국내 자생식물 45종을 선별하였고, 본 연구팀의 선행연구에서 45종의 항산화 활성을 평가한 결과, 다른 종들과 비교하여 우수한 항산화 활성을 보인 밤나무 잎을 본 연구에서 사용하였다. 본 연구에서는 hydrogen peroxide로 산화적 스트레스를 유도한 상태에서 밤나무 잎 추출물의 세포 보호효과, 세포내 항산화 효과 및 항노화 효과를 관찰하였다. 세포 보호효과를 측정한 결과 밤나무 잎 추출물의 농도 유의적으로 세포를 보호하는 효과를 확인하였고, 특히 $100{\mu}g/mL$에서는 양성대조군으로 사용한 항산화 물질인 ascorbic acid 수준까지 세포를 보호하는 효과를 나타내었다. $H_2$-DCFDA 염색을 통한 세포내 항산화 효과를 확인한 결과, 형광현미경과 형광흡광도 측정에서 모두 밤나무 잎 추출물이 농도 유의적으로 세포내 ROS를 저감을 확인하였고, 세포 보호효과와 마찬가지로 농도 $100{\mu}g/mL$에서는 ascorbic acid와 비슷한 수준을 보였다. SA-${\beta}$-galactosidase 염색을 통하여 관찰한 세포노화 억제 효과는 ROS 생성 억제 효과와 유사한 경향으로 밤나무 잎 추출물의 농도 유의적으로 세포노화 억제를 관찰되었다. 이상의 결과를 종합하여 볼 때 밤나무 잎 추출물은 산화적 스트레스로 인한 세포노화를 억제하는 효능을 보였으며, 이는 천연물에서 유래한 항산화 및 항노화 활성 첨가제로 활용도가 매우 넓을 것으로 판단된다.

• 한국미생물·생명공학회지
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• 제45권2호
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• pp.168-177
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• 2017

Strain A28-5는대한민국 제주도 연안의 해수샘플로부터 고체배지내 xylan과 agar를 분해하는 균주로 분리되었다. Strain A28-5는그람 음성균으로 한 개의 polar flagella로 운동성을 갖는 $Na^+$ 이온 요구성 균주로 분석되었다. 또한 ampilcillin과 thiostreptone 등의 항생제에 내성을 보였다. Genome 내 G+C content는 43.96%이고, Menaquinone-7 (MK-7)을 predominant quinone으로 함유하고 있었다. Strain A28-5의 세포벽을 구성하는 주요 지방산은 $C_{16:1}$ ${\omega}7c/iso-C_{15:0}$ 2-OH (23.32%), $C_{16:0}$ (21.83%), $C_{18:1}$ ${\omega}7c$ (17.98%)였다. strain A28-5의 16S rRNA gene sequence는 Catenovulum agarivorans YM01와 가장 높은 상동성(98.94%)을 보였으며, Neighbor-Joining phylogenetic tree 제작을 통해서 Catenovulum agarivorans YM01와 가장 높은 근연관계를 보이는 것을 증명하였다. Catenovulum agarivorans YM01과의 DNA-DNA hybridization 분석을 통하여 A28-5을 Catenovulum 속의 신종으로 분류하여Catenovulum jejuensis A28-5로 명명하였다. 이 균주의 액체배양으로부터 준비된 두 종류의 조효소를 이용한 xylan 또는 agarose와의 효소반응액을 Thin layer chromatography로 분석하여 각각 tetramer와 hexamer의 xylooligosaccharides와 (neo)agarooligosacchardes가 생산되는 것을 확인하였다.

• 한국식품영양학회지
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• 제28권6호
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• pp.1098-1106
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• 2015

This study aimed to investigate antioxidant activities from 11 forest plants, and determine their total phenolics, flavonoids and proantocyanidins contents. In addition, the antioxidant activities were correlated with antioxidant compounds. Among the samples, Cornus officinalis, Castanea crenata, Lindera erythrocarpa, Carpinus laxiflora and Pourthiaea villosa showed significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) ($IC_{50}=21.12{\sim}28.93{\mu}g/mL$) and 2,2'-azino-bis(3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) ($IC_{50}=28.18{\sim}52.55{\mu}g/mL$) radical scavenging ability with reducing power ($IC_{50}=59.91{\sim}93.64{\mu}g/mL$) than other plants; and C. crenata, L. erythrocarpa and Rubus coreanus showed strong nitric oxide (NO) inhibition activity (${\geq}60%$). In addition, L. erythrocarpa, C. laxiflora and P. villosa showed higher oxygen radical absorbance capacity (ORAC) values (${\geq}1,100{\mu}M$ TE/g sample) than other samples. High total phenolic contents were observed in C. crenata (429.11 mg GAE/g), L. erythrocarpa (437.11 mg GAE/g), C. laxiflora (408.67 mg GAE/g) and P. villosa (404.11 mg GAE/g). The DPPH and ABTS radical scavenging activity with reducing power were significantly correlated with total phenolic contents ($R^2=0.71{\sim}0.79$), but total phenolic contents were not correlated with NO inhibition and ORAC ($R^2=0.35{\sim}0.43$). Therefore, these results suggested that C. officinalis, C. crenata, L. erythrocarpa, C. laxiflora and P. villosa are potential natural antioxidative candidate ingredients.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2005년도 학술발표회(2)
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• pp.997-998
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• 2005

• 수산경영론집
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• 제22권2호
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• pp.53-74
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• 1991

Interest on ocean environment has increased with the development of industrialized activities. Public marine resorces are defined broadly to include fish stocks, beaches, marine waters, recreational fishing, biota, waterfowls, shorebirds, seabirds and marine mammals But, it is not easy to analyze compensation for injury to publicly owned marine resources because the claimants do not exist clearly and the economic methodology of damage on public goods is not developed fully. This paper introduces basic idea of welfare economic theory and environmental legislation to the research question : How the economics and law can be applied to the case of damage on publicly owned marine resource. The paper discusses the concepts of willingness to pay (WTP) and willingness to accept (WTA). It is accepted generally that WTA is correct concept of welfare change in the case of damaged public goods. Four methods (compensating variation, equivalent variation, compensating surplus, equivalent surplus of measuring welfare changes are compared. Compensating variation(CV) is the best measure of welfare changes are compared. Compensating variation(CV) is the best measure of welfare changes caused by environmental damage. Vartia (1983) showed CV could be measured from the ordinary demand function using the differential equations. This paper also provides an overview of the emerging U.S. and Korea legal system for compensation for natural resource damages, with particular emphasis on U.S. legal system under Comprehensive Environmen-tal Response Compensation and Liability Act (CERCLA). These regulations are to include two different types of standardized procedures for assessing natural resources injury : Type A or simplified assessment techniques for small releases ; and Type B protocols that would include detailed and extensive assessment methodologies for major releases. Type A procedures are specified by Natural Resources Damage Assessment Model for Coastal and Marine Environment (NRDAM/CME) of the U.S. CERCLA provides a legal 'legitimization for the use of economic-based nonmarket valuation in the courts and have introduced appropriate and accurate nonmarket valuation methods based on willingness to-pay for damage assessment. By briefly reviewing economic theory and environmental legislation, we hope to help provide a better understanding of the compensation process and the economics of publicly owned marine resources in the U.S. and to integrate the economics and law of natural resources valuation into a single comprehensive package in Korea.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2005년도 학술발표회(2)
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• pp.1199-1200
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• 2005

• 한국환경보건학회지
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• 제34권1호
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• pp.62-69
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• 2008

The life cycle assessment method for environmental impact assessment was used, in this study, to assess the production process of wheat flour which is the most important material in the food industry. Environmental impact assessments were compared between that of the Ministry of Environment, Republic of Korea (method I) with that of the Ministry of Commerce, Industry and Energy (method II). Life cycle inventories (LCI) was performed using internal and external databases and the production statistics database of company S. The procedure of life cycle impact assessment (LCIA) was followed in terms of classification, characterization, normalization and weighting to identify the key issues. The impact categories of method I were divided into 8 categories with consideration of : abiotic resources depletion, global warming, ozone depletion, photochemical oxidant creation, acidification and eutrophication. The impact categories of method II were divided into 10 categories with consideration of: abiotic resources depletion, global warming, ozone depletion, photochemical oxidant creation, acidification, eutrophication, human toxicity, freshwater aquatic ecotoxicity, marine aquatic ecotoxicity and terrestrial ecotoxicity.

• 보건행정학회지
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• 제13권3호
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• pp.35-51
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• 2003

This study purports to develop a quality assessment tool for the process of health promotion programs at public health centers(PHC). The draft of the assessment tool developed by the literature was distributed to 242 staffs who were in charge of the health promotion programs at PHCs for evaluating the feasibility of the tool on September and October 2002. The major results of the study were as follows; The quality assessment tool developed in the study consisted of four domains: strategic planning, program management, monitoring and evaluation, and resources and information. The strategic planning dealt with the function of the planning staff and committees, community data analysis, the feasibility of the program, and the approach methods for attaining the goal of the program. The program management included the items on the qualification and power of the program staff. The monitoring and evaluation included the items on the reporting and communication among program units, and feed back after monitoring. Finally, the resources and information dealt with community networking, clients' response, and consulting activity of the staff. The validity of the tools was tested and partly supported by both formative and criterion-related methods. The assessment tools developed in this study could be used by health promotion workers in the self-evaluation of the program quality. In conclusion, the quality assessment tool developed in the study will be a good safeguard for assuring the quality of the process of health promotion programs.

• International Journal of Advanced Culture Technology
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• 제6권3호
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• pp.178-186
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• 2018

Generally, in terms of the development of irrigation scheme, the efficient water resource management that supplies the irrigation water in consideration of the required time and accurate quantity to grow the crop should be conducted. The water resource assessment should precede to supply the irrigation water efficiently. The water resources assessment is divided into the water requirement analysis and the water availability assessment. In case of Korea, the major crop is paddy rice unlike crops of Africa, such as sugarcane, maize, and cassava, etc. Because it is not familiar with the method for upland irrigation development in tropical area, it needs to know the water resources assessment for irrigation scheme development about these crops. The Natama Scheme in Chiradzulu District of the Southern Malawi was selected as study area, which has tropical climate. From the collected meteorological data, the evapotranspiration was analyzed by Penman-Monteith Method and the effective rainfall was analyzed by USDA Soil Conservation Service Method. This study displays the results that for study area, the evapotranspiration varies from 2.80 mm/day to 5.51 mm/day and the effective rainfall varied from 2.1mm to 149.0mm. According to the selected crop (Green Maize, Dry Maize), the unit water requirement (UWR) and water demand (WD) considering the irrigation efficiency, irrigation time and irrigation area were estimated to be $0.00122m^3/s/ha$ and $0.0122m^3/s$ respectively. For the water availability assessment, the runoff of Natama scheme was calculated by specific yield method. The water availability was evaluated through reviewed differences of discharge between $Q80_{intake}$ and Total WD, and the irrigation water can be supplied sufficiently in the existing 10ha of Natama scheme. As a result of reviewing the extensibility of irrigable area, total WD of scheme is $0.02313m^3/s$, and $Q80_{intake}$ is $0.02387m^3/s$ ($Q80_{intake}$ > Total WD). Therefore, Natama scheme can be extended from 10 ha to 17 ha in the dry season in consideration of the $Q80_{intake}$.

• 물과 미래
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• 제45권8호
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• pp.71-77
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• 2012