• Title/Summary/Keyword: Resistant variety

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Complete Genome Sequences of Two Clonal Complex 398 Methicillin-Resistant Staphylococcus aureus Strains Isolated from Patients in Korea

  • Gi Yong Lee;Ji Heon Park;Ji Hyun Lim;Soo-Jin Yang
    • Microbiology and Biotechnology Letters
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    • v.51 no.1
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    • pp.132-133
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    • 2023
  • Clonal complex (CC) 398 community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged worldwide in a variety of livestock animals and humans. We report complete genome sequences of Panton-Valentine leucocidin (PVL) and immune evasion cluster (IEC) gene-positive CC398 MRSA strains isolated from patients in Korea.

Studies on the Relationship Between Chemical Contents of Rice Plants and Resistance to Rice Blast Disease (수도체의 함유성분과 도열병 저항성에 관한 연구)

  • Kwon Shin Han;Oh Jeung Haing;Song Hi Sup
    • Korean journal of applied entomology
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    • v.13 no.1 s.18
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    • pp.33-39
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    • 1974
  • For the investigations on the mechanism of blast disease resistance in the rice variety 'Toilg-il', Ihe chemical contents such as silicate, nitrogen and flavonoid in the leaf blades of tile variety were determined as compared to moderately susceptible variety 'Pung-kwang' A correlation between disease severity of rice blast and the chemical contents in the variety 'Pung-kwang' were also studied. The results showed that silicate and flavonoid contents were decreased inversely with application of nitrogen fertilizer, and negative correlation between silicate and flavonoid contents and disease severity in the field were observed. In the resistant variety 'Tong-il', these chemical contents and silicified cells were significantly higher than in the variety 'Pung-kwang' From these results, it seemed that the high contents of silicate and flavonoid in the leaf blades were related closely to the resistance of the variety 'Tong-il'.

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Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties (주요 오이 품종의 노균병에 대한 저항성 검정)

  • Lee, Jung-Sup;Han, Kyung-Sook;Lee, Seong-Chan;Soh, Jae-Woo
    • Research in Plant Disease
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    • v.19 no.3
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    • pp.188-195
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    • 2013
  • This study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resistance to Pseudoperonospora cubensis, causal agent of cucumber downy mildew. The youngest fully expanded true leaves were found suitable for in vitro screening. Both leaf discs and full leaves could be kept fresh longer when applying 0.2 ${\mu}g/ml$ of gibberellin acid (GA). The incubation temperature of $20^{\circ}C$ was found to be the most suitable temperature for symptom development comparing with 15 and $25^{\circ}C$. Symptom development was faster when contact diseased leaf discs (2 weeks after inoculation) on to fresh leaf samples comparing with using conidia suspension ($10^5$ spores/ml). The numbers of spots in 'C-19' were lower than other varieties. 'C-19' variety was also showed the highest level of downy mildew resistant at $20^{\circ}C$ chamber in 6 days after inoculating with pathogen and displayed 0.90 (under 10%) of the infected rate. However, other varieties displayed susceptible in the pathogen sprayed plots. 'C-19' was the most resistant variety and no lesion was observed. Based on all data, 'C-19' can be a useful variety for the prevention of downy mildew.

Studies on the Nature of Resistance of Rice Plant to Bacterial Leaf Blight Caused by Xanthomonas oryzae (Uyeda et. Ishiyama) Dowson (벼 흰빛잎마름병에 대한 수도 품종의 저항성에 관한 연구)

  • Park Chang Seuk;Cho Yong Sup
    • Korean journal of applied entomology
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    • v.11 no.2
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    • pp.55-60
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    • 1972
  • The study has been carried to investigate the nature of resistance in rice varieties against bacterial leaf blight caused by Xanthomonas oryzae (Uyeda et. Ishiyama) Dowson. In this study, differences on several morphological histological and bacterial growth in rice leaf extract from different varieties were examined. Shirogane and Norm 6 as resistant varieties, Paldal as moderately resistant, and Jinheung, Kimmaze and Suwon 213 as susceptible varieties were used throughout the experiment. 1. Susceptible varieties Jinheung, Kimmaze have more hydathods in flag leaf than resistant varieties but there were no difference between resistant varieties and moderatly resistant varieties. 2. Average length of xylem vessel elements were 50r longer in leaf veins of susceptible varieties Jinheung, Kimnaze and Suwon 213 than those of resistant varieties, but there were no difference among Paldal, Norm 6 and Shirogane. 3. Kimmaze and Suwon 213 have larger diameter of xylem vessel elements than those of other varieties examined. However, it did not differ significantly between resistant varieries and susceptible varieties statistically. 4. Jinheung and Kimmaze have more secondary xylem vessels in root of 6-7th leaf stage than those of Shirogane and Norm 6. Suwon 213, however, showed least number of secondary xylem vessels exceptionally. 5. Leaf extract from resistnat variety Shirogane, suppressed bacterial growth significantly when compared with those from other varieties. 6. Bacterial growth in autoclaved leaf extract from resistant varieties and susceptible varieties did not show any noticeable difference. In general, fresh extract maintained more bacteria than in autoclaved extract after 48 hours of incubation period.

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Changes in the Fitness of Brown Planthopper, Nilapawata lugens Stal (Homoptera: Delphacidae) to Several Resistant Rice Varieties after Multi-generational Selection (누대도태에 의한 벼멸구의 품종적응성의 변화)

  • 송유한;황인철;김진호
    • Korean journal of applied entomology
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    • v.41 no.2
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    • pp.113-121
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    • 2002
  • This study investigated the changes in the fitness of brown planthopper, NilapaHata lugens, to several rice varieties with different resistance background, after multi-generational selection on a resistant rice variety. A susceptible strain of brown planthopper (Dongjin-5) had been reared on the Chungchungbyeo with Bph1 resistance gene for three generations (Chungchung-G3) and six generations (Chungchung-G6), then the fitness change was evaluated by measuring their longevity, fecundity, preferences, and survivorships on the varieties with various background of resistance. After being selected three to six generations on Chungchungbyeo, feeding preference, adult longevity, and fecundity increased, where as nymphal period reduced when they were reared on various varieties with Bph1 gene. The egg Periods were not much different among the varieties fed on, except for the Chungchung-G6 on the rice varieties of Milyang63 (bph2 gene) and Gayabyeo (Bph1+bph2 gene). These results suggest that the susecptible Donajin-5 (Biotype-1) can be easily converted to a resistant biotype-2 capable of overcoming the resistant varieties with Bph 1 gene.

Development of Herbicide Resistant Plant Through Plant Tissue Culture (제초제(除草劑) Butachlor 및 Simetryne에 저항성(抵抗性)인 식물체(植物體) 선발육성(選拔育成))

  • Kim, K.U.;Kim, S.H.
    • Korean Journal of Weed Science
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    • v.7 no.2
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    • pp.200-207
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    • 1987
  • This study was conducted to select and develop herbicide resistant plant through tissue culture. Growth response of seedlings and callis of various rice varieties with Echinochloa species was assessed under the treatment of various rates of butachlor [N-(butoxy methyl)-2-chloro-2', 6'-diethyl acetamide] and simetryne [2,4-bis(ethyl amino)-6-methyl thio-1,3,5-triazine]. Further, succinate dehydrogenase activity was determined in herbicide treated callus to characterize different response of plants to herbicide. Rice variety like Sangpung showed relative resistance in both callus and seedling states against butachlor, indicating maintenance of resistance. However, in the simetryne treatment, the similar response was not observed in callus and seedling state, although there was a great different response among plant materials against simetryne. Rice variety which exhibited resistance in callus and seedling states showed low succinate dehydrogenase inhibition index. Succinate dehydrogenase inhibition index can be used as an important marker characters to differentiate varietal response of plant to herbicide. Rice plant was differentiated from butachlor and simetryne tolerant callus treated at $2.5{\times}10^{-5}$ M is growing under the growth chamber and can be used for resistant source.

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Deterioration of Agronomic Characteristics of Drought-Resistant GM Rice (CaMsrB2-8) (가뭄저항성 GM벼(CaMsrB2-8)의 농업적 특성의 퇴화 가능성 검정)

  • Kim, Bo-Ra;Son, Jin-Hwan;Kim, Hye-Ryun;Ham, Jung-Kwan;Dhungana, Sanjeev Kumar;Park, Soon-Ki;Shin, Dong-Hyun
    • Weed & Turfgrass Science
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    • v.2 no.2
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    • pp.159-163
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    • 2013
  • The purpose of this study is to observe the growth habit and investigate a possibility of cultivating the GM rice (CaMsrB2-8) as a rice cultivar having drought resistance. Germination viability test showed that there was no significant difference between the drought-resistant GM(CaMsrB2-8) and non-GM (Ilmi) rice which was the parent variety at the GM rice. All the seeds of CaMsrB2-8 and Ilmi germinated after 6 days. Viviparous germination was not found in CaMsrB2-8 and Ilmi that was grown in greenhouse at $23{\pm}2^{\circ}C$ with water spraying for 40 days. Ratooning of CaMsrB2-8 and Ilmi was observed in 7-14 days and found uniform in field condition. CaMsrB2-8 seemed to grow faster than Ilmi. But CaMsrB2-8 and Ilmi were similar in 14-21 days. Both CaMsrB2-8 and Ilmi showed low seed shattering and more than 90% grains were ripened. All the seeds scattered in the paddy soil surface were not germinated after passing the winter. This study suggests that the drought-resistant GM rice was not significantly different with the parent variety of Ilmi in many agronomic characteristics such as wildness traits.

A Screening Method on Resistance of Tobacco Plants to Bacterial Wilt (세균성마름병에 대한 담배의 저항성검정 방법)

  • 이영근
    • Journal of the Korean Society of Tobacco Science
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    • v.24 no.1
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    • pp.27-31
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    • 2002
  • Three kinds of inoculation methods, capillary, root cutting and dipping were compared for an efficient way to screening the resistant tobacco variety against bacterial wilt, Ralstonia solanacearum. The pricking a capillary tube contained the pathogenic bacterial suspension(10$^{7}$ cfu/$m\ell$) to an axillary bud of each tobacco plant showed different resistance well between varieties. The less period was required in inoculating work and in disease development for the inoculation method used with capillary tube than for two other inoculation methods tested also.

Breeding of mulberry variety "Shimgang" showing resistance to popcorn disease

  • Sung, Gyoo Byung;Seo, Sang Deog;Kim, Yong Soon;Ju, Wan Taek;Kim, Hyun Bok;Kim, Kee Young
    • International Journal of Industrial Entomology and Biomaterials
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    • v.33 no.2
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    • pp.41-49
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    • 2016
  • We bred a mulberry cultivar named Shimgang, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at four local places(Suwon, Chuncheon, Buan and Jinju) for five years from 2011. This variety 'Shimgang' belongs to Morus Microphylla Buckl. selected from seedlings imported from USA. Shimgang was resistant to popcorn disease and high yielding variety in fruit productivity by 111% compared to control cultivar 'Shimheung(Morus alba L.)' for three years. Although fruits of 'Shimgang' was smaller in size and lower in sugar content of mulberry fruits, it showed stronger resistance than that of 'Shimheung'. It is adaptable to every where except the places where cold and late frost damage happen frequently.

Structure Determination of Macrolactin Compounds with Antibacterial Activities Isolated from Bacillus polyfermenticus KJS-2 (Bacillus polyfermenticus KJS-2에서 분리된 항생물질 마크로락틴류의 구조결정)

  • Kim, Dong-Hee;Kang, Kyung-Ran;Kim, Hyun-Woo;Yoon, Si-Yeol;Kim, Chun-Gyu;Yamaguchi, Tokutaro;Sohng, Jae-Kyung;Kang, Jae-Seon
    • Journal of Life Science
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    • v.20 no.12
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    • pp.1792-1800
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    • 2010
  • In this study, we isolated five macrolactin compounds from a fermentation broth of Bacillus polyfermenticus KJS-2. The macrolactin compounds were structurally identified as macrolactin A (MA), 7-O-malonyl macrolactin A (MMA), 7-O-succinyl macrolactin A (SMA), macrolactin E (ME) and macrolactin F (MF) via a variety of NMR techniques, COZY, DEPT, HMQC and HMBC, and mass and specific rotation assays. The three macrolactin compounds, MA, MMA and SMA, profoundly inhibited the growth of both vancomycin-resistant Enterococci (VREs) and methicillin-resistant Staphylococcus aureus (MRSA), the inhibition of which were estimated via a disc agar diffusion bioassay. MA, MMA, and SMA exhibited antibacterial activities superior to those of vancomycin and teicoplanin.