• Asian Journal of Turfgrass Science
• /
• v.17 no.2_3
• /
• pp.87-97
• /
• 2003

The growth characteristics of creeping bentgrass seeded in the seaside landfill golf course are as follows. 1. As a result of analysis on the chemical characteristics of soil mixture used for turfgrass ground in this experiment, soil pH was 7.9, which is somewhat higher than the creeping bentgrass reference value of pH 5.5∼6.5; T-N(%) was 0.02, which is proper for the reference value, and trace element too lacked. 2. The cultivar with the fastest water infiltration was Seaside II recorded as 226.2cm/sec, while that with the slowest water infiltration was Pennlinks recorded as 141.1 cm/hr which was too faster than the USGA water infiltration reference value of 15∼30cm/hr. For the surface hardness of turfgrass ground with different cultivars, no statistically significant variation was found between the Penncross grass and the Pennlinks recorded as 18.6mm and 19.1 mm, respectively. The soil penetration was the highest in Pennlinks recorded as 7.6kg/$\textrm{cm}^2$ and lowest in Penn A-1 as 6.1kg/$\textrm{cm}^2$. 3. As a result of evaluation on visual quality at the early stage of growth, Penncross showed the most excellent visual quality compared to the others. However, Penn A-1 showed the most excellent visual quality at a late stage of growth around Sep. 17, 2003, and it was also excellent in the evaluation of visual color. Seaside II showed higher density around the root and the longest root length and was highly resistant to salt compared to others, but the initial sprouting rate was not satisfied, and the visual quality in the summer season was inferior to others. 4. As a result of measurement of the traffic injury, Penncross showed the most tolerant to the traffic stress and Pennlinks showed the most susceptible.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.2
• /
• pp.178-184
• /
• 2015

This work aims to utilize wastes from the potato starch industry to produce single-cell protein (SCP) with high lysine content as animal feed. In this work, S-(2-aminoethyl)-_L-cysteine hydrochloride-resistant Bacillus pumilus E1 was used to produce SCP with high lysine content, whereas Aspergillus niger was used to degrade cellulose biomass and Candida utilis was used to improve the smell and palatability of the feed. An orthogonal design was used to optimize the process of fermentation for maximal lysine content. The optimum fermentation conditions were as follows: temperature of 40℃, substrate concentration of 3%, and natural pH of about 7.0. For unsterilized potato starch wastes, the microbial communities in the fermentation process were determined by terminal restriction fragment length polymorphism analysis of bacterial 16S rRNA genes. Results showed that the dominant population was Bacillus sp. The protein quality as well as the amino acid profile of the final product was found to be significantly higher compared with the untreated waste product at day 0. Additionally, acute toxicity test showed that the SCP product was non-toxic, indicating that it can be used for commercial processing.

• Korean Journal of Agricultural Science
• /
• v.42 no.4
• /
• pp.299-304
• /
• 2015

This study was conducted to investigate growth characteristics to develop the technique to select resistant cultivar by wet injury at an early stage through the automatic irrigation maintaining 30, 20, 10 kPa respectively using native variety, Chunpoong, Yunpoong, Gumpoong and Sunun. The aerial growth was decreased at 10 kPa compared to 30 kPa. In addition, the survival rate was decreased by 66.6%, 62.3%, 33.8% at 30, 20, 10 kPa, respectively. The survival rate of Chunpoong and Gumpoong were higher than others at 10 kPa. While root growth characteristics such as root length, root weight, number of lateral root and side root were tended to decrease, root diam was no significant or increased. And the more humid condition is, the more the incidence rate of rusty root and rough skin were tended to increase. The epidermal thickness of Chunpoong and Gumpoong was increased but the figures of native variety, Yunpoong and Sunun were decreased at 10 kPa compared to 30 kPa. But, the tissue stiffness of root was decreased at 10 kPa compared to 30 kPa.

• The korean journal of orthodontics
• /
• v.48 no.5
• /
• pp.339-345
• /
• 2018

Objective: The purpose of this study was to clarify the effects of continuous force application for extrusive tipping movement and occlusal interference on periapical root resorption in the rat mandibular first molar. Methods: We constructed an appliance comprising a titanium screw implant with a cobalt-chromium post as the anchorage unit and a nickel-titanium closed coil spring (50 cN) as the active unit. Force was applied on the mandibular left first molar of rats for 8 (n = 10) and 15 days (n = 10; experimental groups), with the tooth in occlusion. Five rats were included as a non-treated control group to examine the body effect of the appliance. Active root resorption lacunae, identified using tartrate-resistant acid phosphatase, were evaluated in terms of the length, depth, and area. Results: The rat mandibular first molars were mesially tipped and extruded in the occlusal direction. This mesio-occlusal tipping movement and occlusion resulted in the formation of a compression zone and active root resorption lacunae in the distoapical third of the distal roots. However, there was no significant difference in the amount of root resorption between the two experimental groups. The control group did not exhibit any active root resorption lacunae. Conclusions: Periapical root resorption was induced by continuous extrusive tipping force and occlusal interference in rat mandibular molars. These data suggest that we orthodontists had better take care not to induce occlusal interference during our orthodontic treatment.

• Biomedical Science Letters
• /
• v.15 no.4
• /
• pp.301-307
• /
• 2009

Whole-body insulin resistance results largely from impaired insulin-stimulated glucose disposal in skeletal muscle. Our previous studies using differential display and quantitative real-time RT-PCR have shown that a novel cDNA band (DD23) had a higher level of expression in insulin resistant skeletal muscle and it was correlated with whole-body insulin action, independent of age, sex, and percent body fat. In this study, we cloned and characterized DD23. The DD23 sequence is part of the 3'UTR region of the RNA binding motif, single stranded interacting protein (RBMS3). We have cloned the full length cDNA for RBMS3 and identified two splice variants. These variants named DD23-L and DD23-S have 15 and 14 exons respectively and differ from RBMS3 in the 3'UTR significantly. Northern blot analyses showed that an ~8.8 kb mRNA transcript of DD23 was predominantly expressed in skeletal muscle and to a lesser extent in placenta, but not in heart, brain, lung, liver, or kidney, unlike RBMS3. Elevated expression levels of these novel alternatively spliced variants of RBMS3 in skeletal muscle may play a role in whole body insulin resistance.

• Molecules and Cells
• /
• v.25 no.2
• /
• pp.205-210
• /
• 2008

To develop molecular markers linked to the $L^4$ locus conferring resistance to tobamovirus pathotypes in pepper plants, we performed AFLP with 512 primer combinations for susceptible (S pool) and resistant (R pool) DNA bulks against pathotype 1.2 of pepper mild mottle virus. Each bulk was made by pooling the DNA of five homozygous individuals from a T10 population, which was a near-isogenic $BC_4F_2$ generation for the $L^4$ locus. A total of 19 primer pairs produced scorable bands in the R pool. Further screening with these primer pairs was done on DNA bulks from T102, a $BC_{10}F_2$ derived from T10 by back crossing. Three AFLP markers were finally selected and designated L4-a, L4-b and L4-c. L4-a and L4-c each underwent one recombination event, whereas no recombination for L4-b was seen in 20 individuals of each DNA bulk. Linkage analysis of these markers in 112 $F_2$ T102 individuals showed that they were each within 2.5 cM of the $L^4$ locus. L4-b was successfully converted into a simple 340-bp SCAR marker, designated L4SC340, which mapped 1.8 cM from the $L^4$ locus in T102 and 0.9 cM in another $BC_{10}F_2$ population, T101. We believe that this newly characterized marker will improve selection of tobamovirus resistance in pepper plants by reducing breeding cost and time.

• BMB Reports
• /
• v.34 no.4
• /
• pp.293-298
• /
• 2001

Tryptic activation of the 130-kDa Bacillus thuringiensis Cry4B $\delta$-endotoxin produced protease-resistant products of ca. 47 kDa and ca. 21 kDa. The 21-kDa fragment was identified as the N-terminal five-helix bundle (${\alpha}1-{\alpha}5$,) which is a potential candidate for membrane insertion and pore formation. In this study, we constructed the recombinant clone over-expressing this putative pore-forming (PPF) fragment as inclusion bodies in Escherichia coli. The partially purified inclusions were composed of a 23-kDa protein, which cross-reacted with Cry4B antibodies, and whose N-terminus was identical to that of the 130-kDa protein. Dissimilar to protoxin inclusions, the PPF inclusions were only soluble when the carbonate buffer, pH 9.0, was supplemented with 6 M urea. After renaturation via a stepwise dialysis, the refolded PPF protein appeared to exist as an oligomer and was structurally stable upon trypsin treatment. Unlike the 130kDa protoxin, the refolded protein was able to release entrapped glucose from liposomes, and showed comparable activity to the full-length activated toxin, although it lacks larvicidal activity These results, therefore, support the notion that the PPF fragment that consists of ${\alpha}1-{\alpha}5$ of the activated Cry4B toxin is involved in membrane pore-formation.

• Steel and Composite Structures
• /
• v.35 no.3
• /
• pp.305-325
• /
• 2020

Beams of steel frame-tube structures (SFTSs) typically have span-to-depth ratios of less than five. This makes a flexural beam unsuitable for such an application because the plastic hinges at the beam-ends cannot be adequately developed. This leads to lower ductility and energy dissipation capacities of SFTSs. To address this, SFTSs with bolted web-connected replaceable shear links (SFTS-BWSLs) are proposed. In this structural system, a web-connected replaceable shear link with a back-to-back double channel section is placed at the mid-length of the deep beam to act as a ductile fuse. This allows energy from earthquakes to be dissipated through link shear deformation. SFTS and SFTS-BWSL buildings were examined in this study. Several sub-structures were selected from each designed building and finite element models were established to study their respective hysteretic performance. The seismic behavior of each designed building was observed through static and dynamic analyses. The results indicate that the SFTS-BWSL and SFTS have similar initial lateral stiffness and shear leg properties. The SFTS-BWSL had lower strength, but higher ductility and energy dissipation capacities. Compared to the SFTS, the SFTS-BWSL had lower interstory drift, base shear force, and story shear force during earthquakes. This design approach could concentrate plasticity on the shear link while maintaining the residual interstory drift at less than 0.5%. The SFTS-BWSL is a reliable resistant system that can be repaired by replacing shear links damaged due to earthquakes.

• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.4
• /
• pp.564-570
• /
• 2016

The Sal-like 1 gene (Sall1) is essential for kidney development, and mutations in this gene result in abnormalities in the kidneys. Mice lacking Sall1 show agenesis or severe dysgenesis of the kidneys. In a recent study, blastocyst complementation was used to develop mice and pigs with exogenic organs. In the present study, transcription activator-like effector nuclease (TALEN)-mediated homologous recombination was used to produce Sall1-knockout porcine fibroblasts for developing knockout pigs. The vector targeting the Sall1 locus included a 5.5-kb 5' arm, 1.8-kb 3' arm, and a neomycin resistance gene as a positive selection marker. The knockout vector and TALEN were introduced into porcine fibroblasts by electroporation. Antibiotic selection was performed over 11 days by using $300{\mu}g/mL$ G418. DNA of cells from G418-resistant colonies was amplified using polymerase chain reaction (PCR) to confirm the presence of fragments corresponding to the 3' and 5' arms of Sall1. Further, mono- and bi-allelic knockout cells were isolated and analyzed using PCR-restriction fragment length polymorphism. The results of our study indicated that TALEN-mediated homologous recombination induced bi-allelic knockout of the endogenous gene.

• Journal of Nutrition and Health
• /
• v.34 no.5
• /
• pp.541-546
• /
• 2001

This study examined the effect of various types of exercise on bone formation and resorption in rat. Five-week-old male Sprague-Daweley rats were randomly assigned to one of four groups with 10 animals in each; Control, Treadmill, Swimming, Resistance. The exercise regimen consisted of treadmill running at 25m/min, 1 hr per day, 5 days a week, and swimming for 1 hr per day, 5 days a week. Resistant exercise type with weight-bearing was designed to extend lower and upper extremities in order to feed the diet and water. Food intake showed no significant difference among groups but body weight gain and food efficiency were significantly increased in Control group as compared with exercise groups. Femur and tibia length and weight were higher in Control group and the density of therm tended to be higher in exercise groups than Control group, but this difference was not statistically significant. The breaking force of femur was the highest in Swimming group and tibia was the highest in Resistance group among groups, while there was no signigicant difference among the exercise groups. The calcium content of femur was significantly increased in Resistance group than the other groups. Calcium intake and urinary calcium showed no significant difference among groups, while calcium absorption and retention were significantly higher in exercise group than Control group. In conclusion, exercise training enhanced bone formation due to the positive effect on metabolism of calcium and bone which were different according to the types of exercise. (Korean J Nutrition 34(5) : 541∼546, 2001)