• Title/Summary/Keyword: Resistant degree

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Effect of Heat Treatment on In Vitro Hydrolysis Index of Commercial Saengshik (시판 표준 생식의 전분 가수분해지수에 열처리가 미치는 영향)

  • Han, Sung-Hee;Han, Sang-Yoon;Rhee, Chul
    • The Korean Journal of Food And Nutrition
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    • v.21 no.4
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    • pp.470-476
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    • 2008
  • In this study, we assessed the effects of heat treatment on the in vitro hydrolysis indices of commercial Saengshik. Thermal treatment on grain flour and commercial Saengshik increased soluble dietary finer(SDF) and insoluble fiber(IDF), while total dietary fiber(TDF) content remained nearly constant regardless of thermal treatment. Among the samples, COS(Commercial Ohaeng Saengshik) showed the highest TDF and IDF content in raw and heated samples. Additionally, the resistant starch(RS) contents in unheated samples were shown to be high. After heating, the RS levels of all the samples were reduced significantly, by over 12%. The degree of gelatinization in the unheated samples was lower than that of the heated samples, whereas the degree of retrogradation in the unheated samples was higher than that of the heated samples. The hydrolysis indices(HI) of the unheated samples were relatively low, whereas the heated group evidenced high levels. The HI must be affected by content of RS and IDF in samples.

Intraarticular Finding and Clinical Result of Arthroscopic Diagnosis after Manual Manipulation in the Frozen Shoulder (동결견에서 수동 조작 후 관절경 검사시 보인 관절내 소견 및 임상 결과)

  • Moon Young Lae;Park Joon Kwang;Kim Chan Sang
    • Clinics in Shoulder and Elbow
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    • v.3 no.1
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    • pp.33-38
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    • 2000
  • Objectives: The purpose of this study was to grading the severity of intra-articular lesions and evaluate the effects of arthroscopic surgery after manipulation of the resistant frozen shoulder. Materials and Methods : Forty-eight cases from 44 subjects, median age of 53, who underwent arthroscopic surgery after manipulation with minimum follow-up of 12 months were chosen. The UCLA shoulder rating scale was applied, and average scale was 18.2 points. Results: Twelve cases out of our series showed as rotator cuff tear which could produce secondary frozen shoulder. In postoperative follow up, 34 subjects complained of no pain or noctalgia, 5 showed mild degree of pain, 8 with mild degree of remained limited range of motion, and only 1 with no improvement. When viewed with UCLA shoulder rating scale, the most improved aspect was pain, and satisfaction of patient was following. And final average scale was 31.9 points. Conclusion: Our study revealed that arthroscopic surgery after manipulation showed favorably high final scale and patient's satisfaction. Therefore, we recommend this modality for treatment of resistant frozen shoulder in a point of view that the diagnosis and treatment can be done simultaneously.

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Effect of Curcuma xanthorrhiza Gel on Methicillin-Resistant Staphylococcus aureus-Infected Second-Degree Burn Wound in Rats

  • Kesumayadi, Irfan;Almas, Ayyasi Izaz;Rambe, Ilham Nur Hakim;Hapsari, Rebriarina
    • Natural Product Sciences
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    • v.27 no.1
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    • pp.1-9
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    • 2021
  • Methicillin-resistant Staphylococcus aureus (MRSA) infection often complicates burn wounds. Mupirocin is the antibiotic of choice for superficial MRSA infection, and its resistance is on the rise due to its frequent and widespread use. This study aimed to develop and evaluate Curcuma xanthorriza extract (CXE)-containing gel as a topical agent against MRSA-infected second-degree burn wound in rats. CXE was obtained using maceration with 96% ethanol. Xanthorrhizol level, antibacterial, and antioxidant activity were evaluated using a standardized method. In vivo, the wound's healing and bacterial load were evaluated every three days, whereas the histopathology of the wound was examined on day 12 of treatment. One-Way ANOVA and Kruskal-Wallis test were used to analyze the data. In this study, 27.0% and 7.10% of the obtained CXE were xanthorrhizol and curcumin, respectively. Additionally, an IC50 of 64.27 ppm was shown in antioxidant activity measurement, and MIC against MRSA was 5 mg/ml. Treatment with CXE-containing gels showed a significant reduction in bacterial load and proliferation of connective tissue in a dose-dependent manner. In conclusion, CXE-containing gel showed a greater reduction of bacterial load and more advanced wound healing phase than mupirocin.

Decay Resistance of Fire-Retardant Treated Wood

  • Lee, Hyun-Mi;Yang, Jae-Kyung;Kim, Jong-Man
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.6
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    • pp.7-13
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    • 2004
  • In this study, the Korean pine wood (Pinus densiflora Sieb. et Zucc) and Italian poplar wood (Populus euramericana Guinier) was treated with a mixture of monoammonium phosphate (MAP) and boric acid. Their usability as fire retardant and as decay-resistant construction and interior materials were evaluated by testing of chemicals, corrosion rate and absorption rate, weight loss and chemical contents. An experiment was performed to compare treated pine wood and Italian poplar wood. According to the results, Italian poplar wood had higher specific gravity and retention of chemicals than pine wood, and treated wood showed higher decay-resistance than untreated one. Weight loss was less in treated wood than untreated one because the degree of decay was lower in the former than the latter. Corrosion rate and absorption rate met the KS standard for wood preservative performance. The chemical contents analysis was carried out to determine the degree of decay and it was found that the preservative effect of chemical treatment was lower in Italian poplar wood than in pine wood.

RAPID RECOVERY OF PHOTOSYNTHESIS FROM PHOTOINHIBITION IS RELATED TO FATTY ACID UNSATURATION OF CHLOROPLAST MEMBRANE LIPIDS IN CHILLING-RESISTANT PLANTS

  • Moon, Byoung-Yong;Kang, In-Soon;Lee, Chin-Bum
    • Journal of Photoscience
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    • v.5 no.1
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    • pp.1-10
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    • 1998
  • The susceptibility of chilling-resistant spinach plants. and of chilling-sensitive squash plants to photoinhibition was compared in terms of the activity of photosystem II, in relation to the deuce of fatty acid unsaturation of chloroplast membrane lipids. From thylakoid membranes of the plants. monogalactosyl diacylgtycerol, digalactosyl diacylglycerol. sulfoquinovosyt diacylglycerol, and phosphatidylglycerol were seperated as major lipid classes. It was found that the content of cis-unsaturated fatty acids of phosphatidylglycerol was greater by 32% in spinach than that in squash. When leaf disks were exposed to light at 5$\circ$C, 15$\circ$C and 25$\circ$C, photochemical efficiency of photosystem II. measured as the ratio of the variable to the maximum fluorescence of chlorophyll, declined markedly in squash plants, as compared to spinach plants. When leaf disks were exposed to strong light in the presence of lincomycin, an inhibitor of protein synthesis in chloroplasts, photoinhibition was accelerated in the two types of plants. Moreover, lincomycin treatment abolished the differences in the degree of susceptibility to strong light, which had been observed between the two types of plants. When the extent of photoinhibition of photosystem II-mediated electron transport was compared in thylakoid membranes isolated from the two types of plants, there were no differences in the degree of inactivation of photosystem II activity. However, when intact leaf disks were exposed to strong light either at 10$\circ$C or at 25$\circ$C, and then were allowed to recover either at 17$\circ$C or at 25$\circ$C in dim light. chilling-resistant plants such as spinach and pea showed marked recovery from photoinhibition, in contrast to chilling-sensitive plants, such as squash and sweet potato. whose recovery was strongly dependent on the temperature. These findings are discussed in relation to the unsaturation of fatty acids in membrane phosphatidylglycerol. It appears that fatty acid unsaturation of membrane lipids accelerates the recovery of photosystem H from photoinhibition, without affecting the photo-induced inactivation process of photosystem II associated with photoinhibition.

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Resistance of 'Tong-il' variety to the smaller brown planthopper, Laodelphax striatellus Fallen (애멸구에 대한 '통일' 벼의 저항성)

  • Choi S. Y.;Song Y. H.
    • Korean journal of applied entomology
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    • v.13 no.2 s.19
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    • pp.77-82
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    • 1974
  • The variety Tong-il from the IR667 lines have been reported as a variety resistant to the smaller brown planthopper, Laodelphax striatellus Fallen, in Korea. The resistance of Tong-il to the insect, however, have been mainly evaluated from a viewpoint of plant reaction of rice at the seedling stage. In this study, the nature of resistance of Tong-il to the insect was furthermore evaluated from the standpoints of feeding preference, antibiosis and plant tolerance in comparison with the resistant varieties Karsamba Red ASD-7 and Vellailangalayan. and the susceptible variety Jinheung which have been reported to the insect. The smaller brown planthopper much more prefered Tong-il for feeding than the resistant varieties ASD-7 and Vellailangalayan. The degree of antibiosis of Tong-il to the smaller brown planthopper was significantly lower than ASD-7 and Vellailangalayan. The results showed significantly lower nymphal mortality and higher adult emergence in Tong-il and Jinheung than other two resistant varieties. The degree o( tolerance of Tong-il to the insect was also lower than ASD-T and Vellailangalayan. Only. Tong-il seemed to be relatively more tolerable than Jinheung. The reason of showing resistant reaction of Tong-il in the mass screening test might be related with relatively lower feeding preference and more tolerance than Jinheung and other susceptible varieties.

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Stable Expression of TMV Resistance and Responses to Major Tobacco Diseases in the Fifth Generation of TMV CP Transgenic Tobacco

  • Park, Seong-Weon;Lee, Ki-Won;Lee, Cheong-Ho;Kim, Sang-Seock;Park, Eun-Kyung;Choi, Soon-Yong
    • Journal of the Korean Society of Tobacco Science
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    • v.20 no.1
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    • pp.66-70
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    • 1998
  • TMV resistant lines (TRLs) originated from the Blo plant of Nicotiana tabacum cv. NC82 transformed with TMV coat protein cDNA which initially showed delayed disease symptom were selected for increased resistance in each subsequent generation. The result of field experiment of the transgenic tobacco lines in the fifth generation for TMV resistance and their response to other tobacco diseases (black shank, bacterial wilt, and powdery mildew) is described in this report. When fifteen TRLs of the fifth generation were tested for TMV resistance by mechanically inoculating the individual plants, over 95 percent of the plants of 6 lines showed complete resistance even 8 weeks after the inoculation. Average frequency of the resistant plants in TRLs of the fifth generation 8 weeks after the inoculation was 87%. Stable insertion and expression of TMV coat protein cDNA in the fifth generation of the transgenic tobacco plant were confirmed by PCR and immunoblot hybridization, respectively. All TRLs were resistant to the black shank but were susceptible to the bacterial wilt disease and the powdery mildew to the same degree as non-transgenic NC82 was. Therefore, it was indicated that the phenotypes related at least to disease resistance were not changed in the transgenic tobacco. Key words : TMV CP cDNA, TMV resistant tobacco plant, transformation.

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Evaluation of Resistance to Ralstonia solanacearum in Tomato Genetic Resources at Seedling Stage

  • Kim, Sang Gyu;Hur, On-Sook;Ro, Na-Young;Ko, Ho-Cheol;Rhee, Ju-Hee;Sung, Jung Sook;Ryu, Kyoung-Yul;Lee, Sok-Young;Baek, Hyung Jin
    • The Plant Pathology Journal
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    • v.32 no.1
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    • pp.58-64
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    • 2016
  • Bacterial wilt of tomatoes caused by Ralstonia solanacearum is a devastating disease that limits the production of tomato in Korea. The best way to control this disease is using genetically resistant tomato plant. The resistance degree to R. solanacearum was evaluated for 285 tomato accessions conserved in the National Agrobiodiversity Center of Rural Development Administration. These accessions of tomato were originated from 23 countries. Disease severity of tomato accessions was investigated from 7 days to 14 days at an interval of 7 days after inoculation of R. solanacearum under greenhouse conditions. A total of 279 accessions of tomato germplasm were susceptible to R. solanacearum, resulting in wilt and death in 70 to 90% of these plants. Two tomato accessions were moderately resistant to R. solanacearum. Only four accessions showed high resistance against R. solanacearum. No distinct symptom of bacterial wilt appeared on the resistant tomato germplasms for up to 14 days after inoculation of R. solanacearum. Microscopy of resistant tomato stems infected with R. solanacearum revealed limited bacterial spread with thickening of pit membrane and gum production. Therefore, these four resistant tomato germplasms could be used in tomato breeding program against bacterial wilt.

Screening assay for tomato plants resistant to Fusarium oxysporum f. sp. lycopersici race 2 using the expression of the avr2 gene as a selection marker

  • Kim, Mi-Reu;Lee, Jeong Jin;Min, Jiyoung;Kim, Sun Ha;Kim, Dae-Gyu;Oh, Sang-Keun
    • Korean Journal of Agricultural Science
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    • v.48 no.1
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    • pp.151-161
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    • 2021
  • Fusarium wilt disease of tomato plants caused by Fusarium oxysporum f.sp. lycopersici (FOL race2) is one of the most important diseases of tomatoes worldwide. In the competition between tomato and FOL, the FOL can win by overcoming the immune system of tomato plants. Resistant interaction between the FOL race2 and tomato plants is controlled by avirulence genes (AVR2) in FOL and the corresponding resistance genes (I2) in tomato plants. In this study, 7 FOL isolates (KACC) were used to test their pathogenicity, and FOL race2 was selected because it is a broad problem in Korea. The Fol40044 isolates showed the most severe pathogenicity, and the avr2 gene was also isolated and identified. Moreover, to select resistance, 20 tomato varieties were inoculated with the Fol40044, and the degree of pathogenicity was evaluated by analyzing the expression of the avr2 gene. As a result, three resistant tomato varieties (PCNUF73, PCNUF101, PCNUF113) were selected, and the expression of the avr2 gene was much lower than that of the control Heinz cultivar. This result shows that the screening assay is very efficient when the avr2 gene is used as a marker to evaluate the expression level when selecting varieties resistant to tomato wilt disease. Based on these results, it is possible to isolate the I2 gene, which exhibits resistance and molecular biological interactions with the AVR2 gene from the three tomato-resistant varieties. The I2 gene provides breeders more opportunities for Fusarium disease resistance and may contribute to our understanding of their interactions with the FOL and host plant.

AN EXPERIMENTAL STUDY OF THE BOND STRENGTH OF DENTURE TEETH BONDED TO DENTURE BASE MATERIALS (의치용 인공치아와 의치상용 레진간의 결합강도에 관한 실험적 연구)

  • Lee, Joo-Hee;Kim, Chang-Whe;Kim, Yung-Soo
    • The Journal of Korean Academy of Prosthodontics
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    • v.34 no.3
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    • pp.464-474
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    • 1996
  • A principal advantage of a plastic tooth over a porcelain tooth should be its ability to bond to the denture base material. But plastic teeth could craze and wear easily, so more abrasion resistant plastic denture teeth have been developed. To resist abrasion, the degree of cross-linking was increased, but bonding to denture base meterial became more difficult. The purpose of this study was to evaluate the bond strength of plastic teeth and abrasion resistant teeth bonded to heat-curing, self-curing and light-curing denture base material. Denture tooth molds were chosen that had a>8mm diameter. The denture teeth was bonded to three denture base materials and then machined to the same dimensions. Three denture base materials were used as control groups. Prior to tensile testing, the specimens were thermocycled between $5^{\circ}C\;and\;55^{\circ}C$ for 1000cycles. Tensile testing was performed on an Instron Universal testing mechine. Experimental group ; plastic teeth(Justi Imperial)+heat-curing resin(Lucitone 199) plastic teeth(Justi Imperial)+light-curing resin(Triad) plastic teeth(Justi Imperial)+self-curing resin(Vertex SC) abrasion resistant teeth(IPN)+heat-curing resin(Lucitone 199) abrasion resistant teeth(IPN)+light-curing resin(Triad) abrasion resistant teeth(IPN)+self-curing resin(Vertex SC) Control group ; heat-curing resin(Lucitone 199) light-curing resin (Triad) self-curing resin(Vertex SC). The results were as follows : 1. The denture teeth bonded to heat-curing resin showed the cohesive failure and those bonded to the other resins showed adhesive failure. 2. Tensile bond strength of the plastic teeth bonded to self-curing resin was not significantly greater than bonded to light-curing resin(p>0.05). 3. Tensile bond strength of the abrasion resistant teeth bonded to self-curing resin was not significantly greater than bonded to light-curing resin(p>0.05). 4. Tensile bond strength of the plastic teeth to self-curing resin was not significantly different from that of the abrasion-resistant teeth(p>0.05). 5. Tensile bond strength of the plastic teeth to light-curing resin was significantly greater than that of the abrasion resistant teeth(p<0.01).

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