• 농약과학회지
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• 제2권1호
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• pp.53-58
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• 1998

Dicamba의 부작용을 최소화하고, 약효지속효과를 증진하며, 유효성분의 용출속도를 조절할 수 있는 전분을 중합매체로 한 방출조절형 dicamba 입제를 제조하여 기존의 dicamba 액제와 용탈, 토양잔류 및 휘산을 비교하였다. 이들 방출조절형 dicamba 입제는 dicamba 액제에 비하여 약 50% 이상 용탈이 감소되었으며, 토양잔류는 양토에 처리된 dicamba 입제의 토양중 반감기가 $50{\sim}51$일 이었으며, 사양토에 처리하였을때는 $50{\sim}58$일 이었다. 한편 양토 및 사양토에 처리된 dicamba 액제는 각각 24일과 22일 이었다. 또한 dicamba의 휘산성은 방출조절형 dicamba 입제가 dicamba 액제에 비하여 약 10배의 휘산억제효과가 있었다.

• 한국환경농학회지
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• 제16권4호
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• pp.373-381
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• 1997

배추 재배시 살포된 농약의 비산 휘산정도를 알아보기 위해 유기인계인 chlorpyrifos, EPN, pirimiphos-methyl, phenthoate, 유기염소계인 tetradifon, dicofol, 피레스로이드계인 bifenthrin 농약을 이용하여 포장실험을 수행하였다. 이들의 잔류량 분석은 다성분 분석법을 이용하였는데 농약성분에 따라 다르나 회수율은 78${\sim}$92%로 우수하였으며 표준편차도 ${\pm}0.68{\sim}3.52%$로서 재현성이 있었다. 농약의 살포량은 농약사용지침서에서 권하는 량으로 하였으며, 1차실험시에는 바람이 불어오는 첫 구간에 농약을 살포하였고, 2차 실험시에는 풍향에 관계없이 정중앙에 살포하고 방사형으로 시료를 채취하였다. 실험결과 살포농약 대부분이 비산이나 휘산으로 인해 인접 작물에 전이 잔류하는 것을 확인하였다. 살포 중 비산되는 양은 농약성분과 풍향, 거리에 따라 달리 나타났으며 살포구간의 농작물 중 농약잔류량에 비하여 비살포구에서의 농작물 중 농약잔류량 비율은 3m거리에서 평균 0.66%, 6m 거리에서 0.10%, 9m 거리에서 0.05%이었고 근접지역에서는 최고 1.83%에 이르렀다.

• Animal Bioscience
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• 제34권2호
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• pp.265-275
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• 2021

Objective: The study developed mushroom stalk residues as feed additives in the broiler diet for improving the growth performance and immunity of broilers as well as to increase the value of mushroom stalk residues. Methods: In total, 300 ROSS 308 broilers were randomly allocated into fifteen pens with five dietary treatments: i) control, basal diet; ii) CMWM, supplemented with 1% Cordyceps militaris waster medium (CM); iii) CMPE, supplemented with 0.5% CM+0.5% Pleurotus eryngii stalk residue (PE); iv) CMPS, supplemented with 0.5% CM+0.5% Pleurotus sajorcaju stalk residue (PS); v) CMFV, supplemented with 0.5% CM+0.5% Fammulina velutipes stalk residue (FV). Results: The chemical analysis results showed that CM extracts, PE extracts, PS extracts, and FV extracts contain functional components such as polysaccharides and phenols and have both 2, 2-diphenyl-1-picryl-hydrazyl-hydrate scavenging and Ferrous scavenging capacities. The group CMWM saw increased body weight gain and feed conversion rate and the promotion of jejunum villus growth, but there is no significant difference in the intestinal bacteria phase. Antioxidant genes in the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)- antioxidant responsive element pathway among the groups are significantly higher than that of the control group, especially in group CMWM. Conclusion: The mushroom stalk residues have antioxidant functional components, can improve the intestinal health and body weight gain of chickens, and can activate the antioxidant pathway of Nrf2 to increase the heme oxygenase-1 expression. The treatment with 1% CM was the most promising as a feed additive.

• Journal of Veterinary Science
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• 제23권1호
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• pp.14.1-14.11
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• 2022

Background: Carnivore protoparvovirus 1, also known as canine parvovirus type 2 (CPV-2), is the main pathogen in hemorrhagic gastroenteritis in dogs, with a high mortality rate. Three subtypes (a, b, c) have been described based on VP2 residue 426, where 2a, 2b, and 2c have asparagine, aspartic acid, and glutamic acid, respectively. Objectives: This study examined the presence of CPV-2 variants in the fecal samples of dogs diagnosed with canine parvovirus in Bogotá. Methods: Fecal samples were collected from 54 puppies and young dogs (< 1 year) that tested positive for the CPV through rapid antigen test detection between 2014-2018. Molecular screening was developed for VP1 because primers 555 for VP2 do not amplify, it was necessary to design a primer set for VP2 amplification of 982 nt. All samples that were amplified were sequenced by Sanger. Phylogenetics and structural analysis was carried out, focusing on residue 426. Results: As a result 47 out of 54 samples tested positive for VP1 screening, and 34/47 samples tested positive for VP2 980 primers as subtype 2a (n = 30) or 2b (n = 4); subtype 2c was not detected. All VP2 sequences had the amino acid, T, at 440, and most Colombian sequences showed an S514A substitution, which in the structural modeling is located in an antigenic region, together with the 426 residue. Conclusions: The 2c variant was not detected, and these findings suggest that Colombian strains of CPV-2 might be under an antigenic drift.

• 자원리싸이클링
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• 제4권4호
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• pp.12-15
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• 1995

In this paper, some representative waste tire pyrolysis were investigated together with the analysis of the problems associated with the commercialization of various waste tire treatment technologies. Also, R & D results on recovering the oil from the pyrolysis of waste tires, when waste oil was used as a heating medium, were summarized in this study. Experimental results show that the present pyrolysis process has both lower pyrolytic temperature and higher pyrolysis rate than usual one and that the quality of the product oil and residue obtained was relatively even with large availability.

• 한국환경농학회지
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• 제30권1호
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• pp.52-59
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• 2011

본 연구는 취나물 재배 중 chlorfenapyr와 fenarimol을 살포 후 0, 1, 2, 3, 5, 7, 10일에 취나물 시료를 채취하고 각각의 농약을 분석하고 그것의 생물학적 반감기를 산출한 다음에 생산단계 농약 잔류허용기준(PHRLs)을 설정하였다. 취나물 중 chlorfenapyr와 fenarimol은 acetonitrile과 dichloromethane으로 추출과 분배를 거쳐 GC-ECD를 이용하여 분석하였다. Chlorfenapyr와 fenarimol의 정량분석한계(MQL)은 chlorfenapyr는 0.10 mg/kg이었고, fenarimol은 0.02 mg/kg이었다. 그리고 chlorfenapyr의 회수율은 1.0과 5.0 mg/kg의 두 처리수준에서 각각 $94.2{\pm}1.70%$과 $99.0{\pm}1.61%$이었다. Fenarimol의 회수율은 0.2과 1.0 mg/kg에서 각각 $92.1{\pm}2.14%$과 $83.1{\pm}1.98%$이었다. 취나물에서 chlorfenapyr의 생물학적 반감기는 기준량 살포시 3.5일, 배량 살포시에는 3.4일이었다. Fenarimol의 생물학적 반감기는 기준량 살포시 6.0일, 배량 살포시에는 5.9일이었다. 잔류감소 회귀식을 이용하여 계산한 생산단계 농약 잔류허용기준(PHRLs)은 chlorfenapyr가 각각 수확 10일전 13.02 mg/kg이었고, 수확 5일 전에는 6.25 mg/kg으로 제안하였다. 그리고 fenarimol은 각각 수확 10일전에 2.80 mg/kg으로, 수확 5일전에는 1.67 mg/kg으로 제안하였다.

• BMB Reports
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• 제32권3호
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• pp.294-298
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• 1999

Myo-inositol monophosphate phosphatase is a key enzyme in the phosphoinositide cell-signaling system. Incubation of myo-inositol monophosphate phosphatase from porcine brain with N-bromosuccinimide (NBS) resulted in a time-dependent loss of enzyme activity. The inactivation followed pseudo-first-order kinetics with the second-order rate constant of $3.8{\times}10^3\;M^{-1}min^{-1}$. The time course of the reaction was significantly affected by the substrate myo-inositol-1-phosphate, which afforded complete protection against the loss of catalytic activity. Spectrophotometric studies indicated that about one oxindole group per molecule of enzyme was formed following complete loss of enzymatic activity. It is suggested that the catalytic function of myo-inositol monophosphate phosphatase is modulated by the binding of NBS to a specific tryptophan residue at or near the substrate binding site of the enzyme.

• 대한전자공학회논문지
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• 제26권2호
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• pp.87-93
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• 1989

패턴인식과 영상처리 응용에 이용되는 자승오차 패턴 클러스터링 알고리듬은 특징벡터 행렬의 연산에 상당한 처리시간은 요구한다. 그러므로 본 논문은 병렬처리와 파이프라인 특성을 갖는 잉여수체계를 이용한 고속의 자승오차 패턴 클러스터링 프로세서를 제안한다. 제안된 자승오차 패턴 클러스터링 프로세서는 영상분할 실험으로부터 의미있는 영역으로 나눌 수 있는 클러스터의 수에 대하여 만족할 만한 오차를 보이며 80287 수치 연산용 프로세서보다 약 200배 빠름을 보인다. 그 결과 대규모의 데이타를 실시간으로 처리하여야 하는 응용분야에 효과적으로 이용할 수 있음을 확인하였다.

• 펄프종이기술
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• 제29권4호
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• pp.18-27
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• 1997

This study was performed to obtained the optimal delignified condition of exploded wood on the acid hydrolysis with sulfuric acid. Wood chips of pine wood(Pinus desiflora), oak wood(Quercus serrata) and birch wood (Betula platyphylla var. japonica) were treated with a high pressure steam (20-30kgf/$\textrm{cm}^2$, 2-6 minutes). The exploded wood was delignified with sodium hydroxide and sodium chlorite, and then hydrolyzed with sulfuric acid. The result can be summerized as follows ; In the exploded wood treated with sodium hydroxide, the optimal concentration of sodium hydroxide was 1% as content of lignin in the exploded wood. Lignin content of exploded wood treated with sodium chlorite was lower then that sodium hydroxide. The maximum reducing sugar yield of exploded wood treated with 1% sodium hydroxide was lower than non-treated exploded wood. In the case of sodium chlorite treated, the maximum reducing sugar yield was hgher than non-treated exploded wood. Sugar composition of acid hydrolysis solution was composed of xylose and glucose residue, and the rate of glucose residue was increased in high pressure condition.

• Molecules and Cells
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• 제23권2호
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• pp.252-257
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• 2007

Escherichia coli HslVU is an ATP-dependent protease consisting of two heat shock proteins, the HslU ATPase and HslV peptidase. In the reconstituted enzyme, HslU stimulates the proteolytic activity of HslV by one to two orders of magnitude, while HslV increases the rate of ATP hydrolysis by HslU several-fold. Here we show that HslV alone can efficiently degrade certain unfolded proteins, such as unfolded lactalbumin and lysozyme prepared by complete reduction of disulfide bonds, but not their native forms. Furthermore, HslV alone cleaved a lactalbumin fragment sandwiched by two thioredoxin molecules, indicating that it can hydrolyze the internal peptide bonds of lactalbumin. Surprisingly, ATP inhibited the degradation of unfolded proteins by HslV. This inhibitory effect of ATP was markedly diminished by substitution of the Arg86 residue located in the apical pore of HslV with Gly, suggesting that interaction of ATP with the Arg residue blocks access of unfolded proteins to the proteolytic chamber of HslV. These results suggest that uncomplexed HslV is inactive under normal conditions, but may can degrade unfolded proteins when the ATP level is low, as it is during carbon starvation.