• 제목/요약/키워드: Reproductive biology

검색결과 1,527건 처리시간 0.024초

Efficiency of Transgenesis by Using Sperm Mediated Gene Transfer on the Cultured Prepubertal Mouse Testicular Cells

  • Song, Sang-Jin;Cho, Jae-Won;Jun, Jin-Hyun;Byun, Hye-Kyung;Park, Yong-Seog;Chung, Kil-Saeng;Lee, Hoon-Taek
    • 한국동물번식학회:학술대회논문집
    • /
    • 한국동물번식학회 2004년도 춘계학술발표대회
    • /
    • pp.213-213
    • /
    • 2004

  • Exogeneous DNA can reproducibly be delivered by co-injected spermatozoa and this transgenesis method is very efficient protocol. But, mosaic patterns of transgenic embryos and offspring were shown frequently. Whole blastomere integration is important in transgenic animal economics. (omitted)

  • PDF

The effects of sesame oil and different doses of estradiol on testicular structure, sperm parameters, and chromatin integrity in old mice

  • Mohammadzadeh, Masoomeh;Pourentezari, Majid;Zare-Zardini, Hadi;Nabi, Ali;Esmailabad, Saeed Ghasemi;Khodadadian, Ali;Talebi, Ali Reza
    • Clinical and Experimental Reproductive Medicine
    • /
    • 제48권1호
    • /
    • pp.34-42
    • /
    • 2021

  • Objective: Studies of the effects of estrogens on the male reproductive system have emphasized the role of these hormones in male fertility. Sesame oil has many phytoestrogenic compounds and may improve male fertility. This study investigated the effects of sesame oil and different concentrations of estrogen on sperm parameters and DNA integrity in male mice. Methods: Twenty old NMRI (The Naval Medical Research Institute) male mice (40 weeks; weight, 30-35 g) were treated with sesame oil or different concentrations of estrogen (estradiol, 1 and 10 μL/kg/day) or received no treatment (controls). After 35 days, sperm parameters and DNA integrity were assessed and analyzed. Results: Sperm count, progressive motility, and morphology were decreased in the group that received 10 μL/kg of estradiol. A remarkably lower percentage of DNA fragmentation and protamine deficiency were detected in the group that received 1 μL/kg of estradiol. In the groups that received sesame oil and 1 μL/kg of estradiol, the numbers of spermatogonia and Leydig cells were higher than in controls. The combination of sesame oil and 1 μL/kg of estradiol led to improved sperm parameters and chromatin and testicular structure. Conclusion: Based on this study, consumption of sesame oil and a low concentration of estradiol may improve testicular function in older mice.

  • https://doi.org/10.5653/cerm.2020.03524 인용 PDF KSCI