Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2004.06a
- /
- Pages.213-213
- /
- 2004
Efficiency of Transgenesis by Using Sperm Mediated Gene Transfer on the Cultured Prepubertal Mouse Testicular Cells
- Song, Sang-Jin (Laboratory of Animal Reproduction, ARRC, Kon-kuk University, Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital) ;
- Cho, Jae-Won (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospita) ;
- Jun, Jin-Hyun (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospita) ;
- Byun, Hye-Kyung (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospita) ;
- Park, Yong-Seog (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospita) ;
- Chung, Kil-Saeng (Laboratory of Animal Reproduction, ARRC, Kon-kuk University) ;
- Lee, Hoon-Taek (Laboratory of Animal Reproduction, ARRC, Kon-kuk University)
- Published : 2004.06.01
Abstract
Exogeneous DNA can reproducibly be delivered by co-injected spermatozoa and this transgenesis method is very efficient protocol. But, mosaic patterns of transgenic embryos and offspring were shown frequently. Whole blastomere integration is important in transgenic animal economics. (omitted)
Keywords
- Sperm mediated gene transfer;
- Mosaic patterns;
- Spermatogenic cell culture;
- Single blastomere RT-PCR;
- FISH