• Clinical and Experimental Reproductive Medicine
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• 제47권4호
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• pp.277-283
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• 2020

Objective: The sperm DNA fragmentation index (DFI) guides the clinician's choice of an appropriate assisted reproductive technology (ART) procedure. The DFI can be determined using commercially available methodologies, including sperm chromatin dispersion (SCD) kits and sperm chromatin structure assay (SCSA). Currently, when DFI is evaluated using SCD kits, the result is analyzed in reference to the SCSA-derived threshold for the choice of an ART procedure. In this study, we compared DFI values obtained using SCSA with those obtained using SCD and determined whether the difference affects the choice of ART procedure. Methods: We compared SCSA to two SCD kits, CANfrag (n=36) and Halosperm (n=31), to assess the DFI values obtained, the correlations between tests, the technical repeatability, and the impact of DFI on the choice of ART. Results: We obtained higher median DFI values using SCD kits than when using SCSA, and this difference was significant for the CANfrag kit (p<0.001). The SCD kits had significantly higher coefficients of variation than SCSA (p<0.001). In vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) would be chosen for a significantly higher proportion of patients if a decision were made based on DFI derived from SCD rather than DFI determined using SCSA (p=0.003). Conclusion: Our results indicate that SCD kit-specific thresholds should be established in order to avoid the unnecessary use of IVF/ICSI based on sperm DNA damage for the management of infertility. Appropriate measures should be taken to mitigate the increased variability inherent to the methods used in these tests.

• 한국수정란이식학회지
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• 제21권4호
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• pp.331-338
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• 2006

이 연구는 한우의 사육이 집단화, 대형화 되어가고 사육 규모 및 형태가 달라지고 있는 환경에서 번식우 사육 농가를 대상으로 사육 규모와 사육 형태별, 산차별 번식 장애우의 발생 양상을 알아보고, 번식 장애의 발생 유형 및 그 비율을 확인하며, 한우 사육 농가에서 번식우의 영양 상태를 쉽게 판단할 수 있는 신체충실지수에 따른 번식 장애우의 발생 현황을 조사함으로써 이후 한우 번식우 사육을 위한 적정 사육 환경을 규명하고자 수행 하였다. 1. 한우 번식우의 사육 규모별 번식 장애질환의 발생율은 10두 미만 25.2%, 10$\sim$20두 9.4%, 20두 초과 8.3%를 나타내어 사육 규모가 커질수록 번식 장애의 발생율이 유의적으로 낮아졌다(P<0.05). 2. 축사의 형태별 번식 장애 발생율이 개방형 축사에서 8.1%인 반면, 계류형 축사는 14.9%를 나타내어 보행이 원활한 개방형 축사에서의 사육이 한우의 번식 장애 발생율을 유의적으로 낮추었다(P<0.05). 3. 한우 번식우 조사두수 843두 중 101두인 12.0%에서 번식 장애 질환이 발생하였는데, 이들 번식 장애 질환의 발생 유형은 난포 낭종과 지방 괴사가 각각 21.8%로 가장 높았고, 저수태 17.8%, 황체 낭종 9.9%, 미약 발정 7.9 %, 질탈 5.0%, 자궁 경관 협착 4.0%, 무발정 4.0%, 난소 위축 3.0%, 난소 기능 휴지 3.0%, 자궁 내막염 1.0%, 자궁 축농증 1.0%의 순으로 분포되었다. 4. 산차별 번식 장애 발생 비율은 2산차의 한우에서 27.4%로 가장 높은 비율을 나타낸 반면, 미경산우가 2.7%의 낮은 번식 장애율을 보였으며, 3산 12.3%, 4산 8.2%로 2산 이후 그 비율이 낮아지는 경향을 나타내었고, 기타 6산 이상(6산$\sim$12산)에서 37.0%의 비율로 분포하였다. 5. 신체 충실 지수에 따른 번식 장애우의 발생 비율은 1.5에서 80.0%, 2.0에서 8.8%, 2.5에서 9.4%, 3.0에서 10.0%, 3.5에서 33.3%, 4.0에서 57.1%를 나타내어 허약한 1.5 및 비만한 4.0에서 높게 나타난 반면 적정 수준인 2.5 및 3.0에서는 아주 낮게 나타났다.

• Clinical and Experimental Reproductive Medicine
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• 제30권3호
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• pp.249-254
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• 2003

Ectopic pregnancy is a common medical problem that is difficult to diagnose and potentially may lead to significant mortality or morbidity. The incidence of ectopic pregnancy is definitely increasing ue to the rise in pelvic inflammatory disease (PID), pelvic surgery, intrauterine device (IUD), and assisted reproductive technologies, such as in vitro fertilization and embryo transfer (IVF-ET). Combined ectopic gestations are much rare and their true incidence is unknown. Multiple ectopic gestations may occur in a variety of locations. The majority involve one or both fallopian tubes. We report a case of combined tubal and cervical pregnancies, and discuss their management.

• Clinical and Experimental Reproductive Medicine
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• 제39권2호
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• pp.46-51
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• 2012

Fertility preservation (FP) is an effort to retain the fertility of cancer patients, and as an emerging discipline, it plays a central role in cancer care. Because of improvement in diagnostic and therapeutic strategies, an increasingly large number of patients are surviving with cancer. FP specialists should make an effort to spread the significance of FP among reproductive women with cancer and provide appropriate education both for associated physicians and for cancer patients who wish to preserve their fertility. Physicians who take part in the initial diagnosis and management of cancer should consider the importance of early referral of young cancer patients to FP specialists and take care of those patients by providing timely information and appropriate counseling. Individualized treatment strategies should be delivered depending on the patient's situation with appropriate team approach.

• Asian-Australasian Journal of Animal Sciences
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• 제26권5호
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• pp.630-637
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• 2013

To determine the effects of dry period (DP) length on milk yield, milk composition, some blood metabolites, complete blood count (CBC), body weight and score and follicular status, twenty five primiparous and multiparous Holstein cows were assigned to a completely randomized design with DP-60 (n = 13) and DP-20 (n = 12) dry period lengths. Cows in the DP-60 produced more milk, protein, SNF, serum non-esterified fatty acids (NEFA) and beta hydroxyl butyrate acid (BHBA) compared with cows in DP-20 ($p{\leq}0.05$). Serum glucose, blood urea nitrogen (BUN), urea, and glutamic oxaloacetic transaminase (GOT) were all similar among the treatments. Body Condition Score (BCS), body weight (BW), complete blood count (CBC) and health problems were similar between the treatments. Diameter of the first dominant follicle and diameter of the dominant follicle on d 14 were different among the treatments. Thus, results of this study showed that reducing the dry period length to DP-20 had a negative effect on milk production, milk composition and reproductive performance in Holstein dairy cows.

• 한국작물학회지
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• 제45권5호
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• pp.282-287
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• 2000

Soybean [Glycine max (L.) Merrill] crops, grown in a rice soybean rotation, can suffer when grown in soil with excessive moisture. The objective of this work were to determine the reduction in growth and yield, responses of vegetative and reproductive growth of soybean to excessive soil moisture achieved by prolonged irrigation. Responses of different cultivars were determined at growth stages from V6 to R8 to clarify the sensitive growth stages or characteristics to excessive soil moisture. Cultivar differences in response to excessive soil moisture condition were conspicuous in seed dry weight and harvest index (HI) but not in the response of seed number or pod number per plant. The timing of irrigation causing the condition of excessive soil moisture influenced the vegetative or reproductive traits. Soybean plants were more affected by irrigation commencing at the pre-flowering than at the post-flowering stage. Post-flowering irrigation did not reduce growth of vegetative organs significantly; in fact the growth of stems and leaves was facilitated by the prolonged irrigation commencing at flowering. Differences between cultivar response to prolonged irrigation were assumed to relate to the reduced amount of assimilates translocated to the reproductive organ.

• Clinical and Experimental Reproductive Medicine
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• 제49권2호
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• pp.101-109
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• 2022

Objective: Y chromosome microdeletions are the second most common genetic cause of male infertility after Klinefelter syndrome. The aim of this study was to determine the patterns of Y chromosome microdeletions among infertile Mongolian men. Methods: A descriptive study was performed on 75 infertile men from February 2017 to December 2018. Y chromosome microdeletions were identified by polymerase chain reaction. Semen parameters, hormonal levels, and testis biopsy samples were examined. Results: Among 75 infertile men, two cases of Y chromosome microdeletions were identified. The first case had an AZFa complete deletion and the other had an AZFc partial deletion. This study found that the proportion of Y chromosome microdeletions among infertile Mongolian men was 2.66%. Conclusion: The findings can be applied to in vitro fertilization and assisted reproductive technology, and our results will help clinicians improve treatment management for infertile Mongolian couples.

• Asian-Australasian Journal of Animal Sciences
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• 제28권3호
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• pp.451-455
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• 2015

The buffalo is an important livestock resource in several countries of South Asia and the Mediterranean regions. However, reproductive efficiency is compromised due to known problems of biological and management origins, such as lack of animal selection and poor nutrition. Under optimal conditions puberty is attained at 15 to 18 months in river buffalo, 21 to 24 months in swamp buffalo and is influenced by genotype, nutrition, management and climate. However, under field conditions these values deteriorate up to a significant extant. To improve reproductive efficiency, several protocols of oestrus and ovulation synchronization have been adopted from their use in commercial cattle production. These protocols yield encouraging pregnancy rates of (30% to 50%), which are comparable to those achieved in buffaloes bred at natural oestrus. The use of sexed semen in buffalo heifers also showed promising pregnancy rates (50%) when compared with conventional non-sexed semen. Assisted reproductive technologies have been transferred and adapted to buffalo but the efficiency of these technologies are low. However, these latest technologies offer the opportunity to accelerate the genetic gain in the buffalo industry after improving the technology and reducing its cost. Most buffaloes are kept under the small holder farming system in developing countries. Hence, future research should focus on simple, adoptable and impact-oriented approaches which identify the factors determining low fertility and oestrus behaviour in this species. Furthermore, role of kisspeptin needs to be explored in buffalo.

• Clinical and Experimental Reproductive Medicine
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• 제37권4호
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• pp.267-291
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• 2010

최근 동결기술이 발달하면서 다양한 목적에 따라 초기 발생단계, 특히 수정 전후의 난자나 수정란의 생명을 연장하는 것이 가능해졌다. 이러한 난자나 수정란의 보존기술은 인간의 수정능력을 배가시키거나 임신조절에서 응용되고 있으며, 동물에서는 우수한 유전자원의 보존과 운영, 저렴한 국제간 운송수단, 그리고 생식보조기술과 유전공학 등의 연구에 필요한 생식세포의 공급하는 데서도 중요하게 활용되고 있다. 최근 개발된 완만동결과 유리화 동결방법은 난자와 수정란을 장기간 동결하여 보존하는데 활용하는 주요 기술이다. 이러한 방법들은 각각 장점과 단점을 가지고 있지만, 상당한 수준의 효율성이 입증되어 실용화되어 있는 실정이다. 무엇보다도 유리화 방법은 완만동결 방법보다 13년이나 늦게 개발되었으나 보다 우수한 기술로 인정을 받고 있다. 비록 유리화 동결은 아직 대한 상반된 의견과 오염문제가 있지만 인간과 동물의 생식보조기술로 활용되는 빈도가 점차 많아지고 있는 실정이다. 따라서 본 원고에서는 먼저 난자와 수정란의 동결보존에 대한 기초적인 기술에 대해서 고찰한 다음, 유리화 동결에 관 한 최근의 연구동향에 대해서 종합적으로 검토하고자 한다.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2006년도 제51차 추계학술대회
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• pp.160-160
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• 2006