• 한국문화재보존과학회:학술대회논문집
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• 2005.11a
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• pp.55-60
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• 2005

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.5
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• pp.689-694
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• 1999

This experiment was carried out to isolate the partial bovine (Bos Taurus coreanae) myogenic factor encoding gene, MyoD, using the rat myogenic factor (MyoD) gene sequence and to compare the gene sequence between another myogenic factor (Myf 5) and MyoD gene of the bovine. To make the probe and isolate the MyoD gene, PCR was performed to amplify rat and bovine MyoD gene including exon I, II and intron I. The homology between mouse and bovine MyoD is high; bovine MyoD gene shows 17 different gene sequence region compared to rat MyoD. Among those, two regions have significant differences; one is the exon I part between 2834 and 2850 bp, the other is intron part between 3274 and 3303 bp of the mouse. At this region homology was 40% in the former and 50% in the latter. Homology between bovine MyoD and Myf5 was 83% in the exon 1. Especially exon I in the Myf5 602-617 bp and 651-683 bp have significant differences. These results suggest that MyoD gene have a similar gene structure in mouse and bovine and MyoD and Myf5 of the bovine, at least in part, have a similar expression and activity.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.6
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• pp.739-747
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• 2000

This experiment was conducted to evaluate the influence of dimethyl-sulfoxise (DMSO, 0, 5, 50, 100 and $500{\mu}M$) on the motility and acrosome reaction of the frozen-thawed spermatozoa from 3 different bulls (Bull A, Band C). Also we evaluated the developmental capacity of bovine embryos fertilized in a medium containing DMSO at various concentrations. DMSO had negligible effects on the sperm motility and acrosome reaction in all three bulls. However, the development rates from 2 to 16 cells stage on the 3rd day after insemination with 50, 100 and $500{\mu}M$ DMSO in Bull-B, and up to the blastocyst stage fertilized with 5, 50, 100 and $500{\mu}M$ in Bull-A were significantly higher (p<0.05) than those of control ($0{\mu}M$ DMSO) group from each bull. Furthermore, the rates of blastocysts per cleaved embryos of 5 to $500{\mu}M$ DMSO group in Bull-A and of 5 to $100{\mu}M$ DMSO in Bull-C were also significantly higher (p<0.05) than those for their $0{\mu}M$ groups, respectively. These results indicate that DMSO at micromol level used for in vitro fertilization might stimulate the development of embryos for some bulls.

• The Journal of Korean Academy of Prosthodontics
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• v.38 no.2
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• pp.191-199
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• 2000

Wettability of addition silicone impression material is very important property for making an accurate restoration. This study examined the impression quality in clinical condition and the wettability of impression and die material. Four commercially available addition silicone impression material (Express, Examix, Contrast, Perfect) and three die materials (Die-Keen, Vel-Mix, Fuji-Rock) were studied. A total of 50 putty/wash and heavy body/wash impressions of wet intact permanent molar teeth were examined for definition of the gingival sulcus reproduction and then classified in quality ranking. The percentage of the sulcus reproduction ability of each material was calculated from the sulcus depths of cross-sectioned epoxy resin casts from the impressions and clinically measured sulcus depths, The same impression materials were used to produce 3 groups of die stone casts form void entrapment die had been exposed to milk. Voids in the impression body and stone casts ere counted under a stereoscopic microscope. From the experiment, the following results were obtained : 1. Sulcus reproduction ability of additional silicone impression material were diminished in order of Express, Examix, Perfect, Contrast. The significant difference was found between Perfect and other material. Heavy body/wash combination was superior In putty/wash method n Perfect impression material. 2. In direct observation, Contrast showed least void in impression body but correlations ere not found between sulcus reproduction and void production. 3. In void entrapment laboratory test, wettability were diminished in order of Examix, Contrast, Express, Perfect. Clinical impression recording seems not to correlate with laboratory test. 4. The wettability of die material to impression material was not different in Express, Examix, Contrast. But, in Perfect, Die-Keen had superior wettability to others.

• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.191-196
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• 1996

The objective of this study were to investigate the effects of culture media and additives on the development of bovine in vitro matured(IVM) and in vitro fertilized(IVF) oocytes. In experiment 1, bovine oocytes were cultured in droplets of TC 199 supplemented with 10% fetal bovine serum(FBS) with or without hormones (5$\mu\textrm{g}$/ml FSH, 5$\mu\textrm{g}$/ml LH, 1$\mu\textrm{g}$/ml E2). Cleavage rates of embryos cultured for 40~44hrs after IVF were higher when embryos were cultured in TC 199 supplemented hormones (68.1%, 921/35) than without hormones (52.7%, 77/146), but the percentages of development beyond morulae stage were not difference (20.7%, 19.4%). In experiment 2, the effects of various media such as TC 199, synthetic oviduct fluid(SOF), CR1aa with different energy source (fatal bovine serum, FBS; bovine serum albumin, BSA) on developmental capacity of IVM/IVF bovine embryos were investigated. The developmental rates into morulae and blastocysts were 27.1, 10.7, 6.3 and 0%, respecitvely, in CR1aa plus 3mg/ml BSA, SOF plus 10% FBS, TC 199 plus 10% FBS, SOF plus 3mg/ml BSA. In experiment 3, the comparisons of bovine embryos developed to morulae and blastocysts in different culture media (TC 199, SOF, CR1aa, Menezo's B2) were investigated. The developmental capacity beyond morulae stage were 32.9, 26.6, 11.1 and 7.1%, respectively, in Menezo's B2 plus BSA, CR1aa plus BSA, SOF plus BSA, TC 199 plus FBS medium. The cell numbers of the blastocyst were not different in different cultrue media. In experiment 4, bovine embryos were co-cultured with vobine oviduct epithelial cells(BOEC) in TC 199 plus FBS, SOF plus BSA, CR1aa plus BSA, Menezo's B2 plus BSA. The morula and blastocyst rates were 44.7, 32.9, 26.0 and 23.3%, respectively, in CR1aa TC 199, SOF, and Menezo's B2 medium. The cell numbers of the blastocyst were similar to those of blastocyst developed in different culture media.

• Korean Journal of Animal Reproduction
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• v.18 no.2
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• pp.105-111
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• 1994

The effects of human or mouse leukemia inhibitory factor(hLIF or mLIF) were examined as a means of increasing the development of in vitro matured(IVM) and in vitro fertilized (IVF) oocytes into morulae or blastocysts. Cell numbers of blastocysts were also counted using Hochest dye staining. Two-to 8-cell embryos derived from bovine IVM/IVF oocytes were cultured 5 to 6 days in CRI aa with or without mLIF or hLIF. All culture media were contained 3mg/ml bovine serum albumin. In experiment 1, the proportion of embryos developed to morulae and blastocysts in CRI aa containing 5,000U/ml mLIF(37.8%) was slightly higher than those of CRIaa containing 1,000U/ml mLIF(34.6%) and 0 U/ml mLIF(27.4%; P>0.05). In experiment 2, 0, 1,000 and 5,000U/ml of hLIF added to CR1aa media yielded 27.6%, 43.0% and 35.5% morulae and blastocysts, respectively(p>0.05). These were no significant increases in cell number among treatments(p>0.05). These results were indicating that mLIF or hLF can increase the proportion of embryos that develop into morulae and blastocysts without and increase in the cell number.

• Korean Journal of Animal Reproduction
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• v.27 no.4
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• pp.281-285
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• 2003

This study was carried out to find out the changes on serum concentrations of estradiol-17$\beta$, progesterone in primiparous Duroc, Landrace and Yorkshire sows weaned at 7 or 21 days. Also, we compared the litter size at birth and weaning among the breeds weaned after lactation for 7 or 21 days. The estradiol-17$\beta$ concentrations among the breeds were 6.9∼8.8 pg/ml and 6.4∼8.8 pg/ml after lactation for 7 or 21 days, respectively. The progesterone concentrations ranged from 0.3 ng/ml to 1.6 ng/ml. Duroc sow showed higher progesterone concentration compared with Landrace and Yorkshire sows weaned after lactation for 7 or 21 days. Also, we found out that litter size at birth and weaning, respectively, did not show any differences between day 7 and day 21 of lactation. From the facts mentioned above, it was suggested that very early weaning systems could work with no apparent adverse effect on prolificacy.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.10
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• pp.1329-1334
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• 2011

The Hinai-dori is a breed of chicken native to Akita Prefecture, Japan. An $F_2$ resource population produced by crossing low- and high-growth lines of the Hinai-dori breed was analyzed to detect quantitative trait loci (QTL) for growth traits. Highly significant QTLs for body weight at 10 and 14 weeks of age and average daily gain between 4 and 10 weeks and between 10 and 14 weeks of age were accordingly mapped in a common region between ADL0198 and ABR0287 on chromosome 1 and between MCW0240 and ABR0622 on chromosome 4, respectively. A significant QTL for body weight at 4 weeks of age and a significant QTL for average daily gain between 0 and 4 weeks of age were mapped for the first time to the same region flanking ABR0204 and ABR0284 on chromosome 1. These QTLs are good candidates for application in the development of marker-assisted selection strategies for increasing growth efficiencies in the Hinai-dori breed and native breeds of chickens in Asia.

• Korean Journal of Animal Reproduction
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• v.17 no.1
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• pp.1-6
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• 1993

This experiment was conducted to determine the effects of gossypol injection spermatogenesis of mice. Gossypol was injected into the stroma of testes(TS) and the doses of gossypol injected were 5, 10 and 15mg per kg of body weights, respectively. The number of sperm and the weight of testes were gradually reduced(P<0.01) from 2 to 4 weeks after gossypol treatment in all groups of mice treated with gossypol, compared with the control group. The rates of malformation(loss of proacrosome, damage of midpiece and breaking of tail) of sperm were significantly(P<0.01) increased at 2 and 3 weeks after the injection of 10 or 15mg of gossypol. However, the weight of testes and the number of normal sperm were gradually increased and the malformation rate of sperm was decreased between 4 and 6 weeks after injection of 5mg of gossypol. The results of this experiment indicated that probably ireeversible suppression of spermatogenesis could be brought about easily and immediately by the single injection of gossypol into TS.

• Korean Journal of Animal Reproduction
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• v.22 no.1
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• pp.81-87
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• 1998

This experiment was carried out with 12 Korean native heifers(8~12month old, body weight, 160~240kg) raised at a farm of Chang-Soo Livestock Cooperatives to evaluate the effects of rGH(recombinant growth hormone) on serum concentrations of growth hormone, estrogen, and IGF-I, weight gain, teat volume gain and processing enzyme activity of IGF-I, binding protein III at 28 day intervals. Animals used were injected with 250mg rGH at 14 day intervals from December to Ferbruary in 1994. The significant difference was found in the group of treatment on the 4th week in the endogenous GH(p<.01) and 8th week in estrogen and IGF-I(p<.05) after injectin of rGH in Korean native heifers. There were significant differences between control group and treatment group in weight and teat volume on 8th week after treatment(p<.05). Processing enzyme activity before injection of rGH were low. However, heifers injected with 250mg of rGH showed that processing enzyme activity of IGF binding protein was highly increased throughout the experiment. Present results suggest that injection of exogenous rGH to heifers can increase the growth performance and udder development of Korean native heifers by the endogenous hormonal changes.