Effects of Gossypol Injection into the Stroma of Testes on Spermatogenesis in Mouse

생쥐 정소 실질내 Gossypol 투여가 조정기능에 미치는 영향

This experiment was conducted to determine the effects of gossypol injection spermatogenesis of mice. Gossypol was injected into the stroma of testes(TS) and the doses of gossypol injected were 5, 10 and 15mg per kg of body weights, respectively. The number of sperm and the weight of testes were gradually reduced(P<0.01) from 2 to 4 weeks after gossypol treatment in all groups of mice treated with gossypol, compared with the control group. The rates of malformation(loss of proacrosome, damage of midpiece and breaking of tail) of sperm were significantly(P<0.01) increased at 2 and 3 weeks after the injection of 10 or 15mg of gossypol. However, the weight of testes and the number of normal sperm were gradually increased and the malformation rate of sperm was decreased between 4 and 6 weeks after injection of 5mg of gossypol. The results of this experiment indicated that probably ireeversible suppression of spermatogenesis could be brought about easily and immediately by the single injection of gossypol into TS.