• Title/Summary/Keyword: Representative Strain

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Genealogical Relationship between Pedigree and Microsatellite Information and Analysis of Genetic Structure of a Highly Inbred Japanese Black Cattle Strain

  • Sasazaki, S.;Honda, T.;Fukushima, M.;Oyama, K.;Mannen, H.;Mukai, F.;Tsuji, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.10
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    • pp.1355-1359
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    • 2004
  • Japanese Black cattle of Hyogo prefecture (Tajima strain) are famous for its ability to produce high-quality meat and have been maintained as a closed system for more than 80 years. In order to assess the usefulness of microsatellite markers in closed cattle populations, and evaluate the genetic structure of the Tajima strain, we analyzed representative dams of the Tajima strain comprised of the substrains Nakadoi and Kinosaki. Genetic variability analyses indicated low genetic diversity in the Tajima strain. In addition, a recent genetic bottleneck, which could be accounted for by the high level of inbreeding, was detected in both substrains. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using pedigree and microsatellite information. Two phylogenetic trees were constructed from microsatellite and pedigree information using the UPGMA method. Both trees illustrated that most individuals were distinguished clearly on the basis of the two substrains, although in the microsatellite tree some individuals appeared in clusters of different substrains. Comparing the two phylogenetic trees revealed good consistency between the microsatellite analysis tree and the pedigree information. The correlation coefficient between genetic distances derived from microsatellite and pedigree information was 0.686 with a high significance level (p<0.001). These results indicated that microsatellite information may provide data substantially equivalent to pedigree information even in unusually inbred herds of cattle, and suggested that microsatellite markers may be useful in revealing genetic structure without accurate or complete pedigree nformation. Japanese Black cattle of Hyogo prefecture (Tajima strain) are famous for its ability to produce high-quality meat and have been maintained as a closed system for more than 80 years. In order to assess the usefulness of microsatellite markers in closed cattle populations, and evaluate the genetic structure of the Tajima strain, we analyzed representative dams of the Tajima strain comprised of the substrains Nakadoi and Kinosaki. Genetic variability analyses indicated low genetic diversity in the Tajima strain. In addition, a recent genetic bottleneck, which could be accounted for by the high level of inbreeding, was detected in both substrains. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using pedigree and microsatellite information. Two phylogenetic trees were constructed from microsatellite and pedigree information using the UPGMA method. Both trees illustrated that most individuals were distinguished clearly on the basis of the two substrains, although in the microsatellite tree some individuals appeared in clusters of different substrains. Comparing the two phylogenetic trees revealed good consistency between the microsatellite analysis tree and the pedigree information. The correlation coefficient between genetic distances derived from microsatellite and pedigree information was 0.686 with a high significance level (p<0.001). These results indicated that microsatellite information may provide data substantially equivalent to pedigree information even in unusually inbred herds of cattle, and suggested that microsatellite markers may be useful in revealing genetic structure without accurate or complete pedigree information.

Evaluation of Dynamic Soil Properties Using Dynamic Tests (동적시험에 의한 동적지반특성 평가)

  • Lee, Myung Jae;Shin, Jong Ho;Kang, Ki Young;Chon, Chun Su
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.10 no.2
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    • pp.91-102
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    • 1990
  • The representative tests in this study are performed at a selected site which has the soil layers to analyze the safety and economy of the dynamic analysis for the variable soil conditions. Crosshole test and downhole test of small strain level tests and triaxial test of large strain level test are performed in the soil layers, and in the rock layers, crosshole test and downhole in-situ tests and laboratory sonic test are performed to measure the dynamic shear modulus, damping ratio, and Poisson$\acute{s}$ ratio of the soil and the rock. The correlations between the dynamic soil properties from the tests and the basic soil properties are determined through the regression analysis. The representative design value of the soil is determined by probability analysis of the test results. It is determined from the nonlinear stress-strain model in soils, and the value at small strain level is computed in rocks according to the distribution of the type of soils and the affecting variables. The constitutive value is systematized to be utilized in the analysis of the test results, and computation of the input soil data.

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Numerical Study of Interaction between Hydrogen and Hydrocarbon Flames (수소화염과 탄화수소화염의 상호작용에 관한 수치계산 연구)

  • Oh, Chang-Bo;Lee, Eui-Ju
    • Journal of the Korean Society of Safety
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    • v.25 no.2
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    • pp.12-17
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    • 2010
  • Numerical simulations were performed for the prediction of the flame structure during the interaction between hydrogen and hydrocarbon flames. A counterflow flow geometry was introduced to establish the interacting two flames. Methane was used as a representative hydrocarbon fuel in this study. A well-known numerical code for the counterflow flame, OPPDIF, was used for the simulations. The detailed chemistry was adopted to predict the flame structure reasonably. The interaction of two one-dimensional premixed flames established in counterflow burner was investigated with the global strain rate and velocity ratio. It was found that the maximum temperature located near the methane flame surface while the heat release rate of methane was lower than hydrogen flame. The flame thickness become narrow with increasing the velocity ratio while the global strain rate was fixed. The local strain rate and heat release rate at the methane flame surface were correlated with the global strain rate, while those at the hydrogen flame were not correlated with the global strain rate. However, the maximum temperature of the interacting flames was correlated with the global strain rate.

Preliminary data analysis of surrogate fuel-loaded road transportation tests under normal conditions of transport

  • JaeHoon Lim;Woo-seok Choi
    • Nuclear Engineering and Technology
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    • v.54 no.11
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    • pp.4030-4048
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    • 2022
  • In this study, road transportation tests were conducted with surrogate fuel assemblies under normal conditions of transport to evaluate the vibration and shock load characteristics of spent nuclear fuel (SNF). The overall test data analysis was conducted based on the measured acceleration and strain data obtained from the speed bump, lane-change, deceleration, obstacle avoidance, and circular tests. Furthermore, representative shock response spectrums and power spectral densities of each test mode were acquired. Amplification or attenuation characteristics were investigated according to the load transfer path. The load attenuated significantly as it transferred from the trailer to the cask. By contrast, the load amplified as it transferred from the cask to the surrogate SNF assembly. The fuel loading location on the cask disk assembly did not exhibit a significant influence on the strain measured from the fuel rods. The principal strain was in the vertical direction, and relatively large strain values were obtained in spans with large spacing between spacer grids. The influence of the lateral location of fuel rods was also investigated. The fuel rods located at the side exhibited relatively large strain values than those located at the center. Based on the strain data obtained from the test results, a hypothetical road transportation scenario was established. A fatigue evaluation of the SNF rod was performed based on this scenario. The evaluation results indicate that no fatigue damage occurred on the fuel rods.

Differences in electric potential of meridian system -Comparing electrical potentials of patients with Lumbago due to strain and contusion- (좌섬요통 환자의 12경맥 전위측정 연구)

  • Choi, Hwan-soo;Nam, Bong-hyun
    • Journal of Acupuncture Research
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    • v.21 no.5
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    • pp.101-111
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    • 2004
  • Objective : Assuming that the characteristic of meridian system has been similar to this of electric potentials in human body and that measurements of electric potential at well(井穴) and sea (合穴) points in branches of the twelve meridians(WSBTM) will be representative of measurements of the twelve meridians, to measure the electric potentials of 13 patients with Lumbago due to strain and contusion(좌섬요통<挫閃腰痛>, LSC), to find out the characteristic of meridian system in patients with LSC. Methods : Electric potentials of well and sea points in the meridians in twenty one patients with the pain in the lion diagnosed as LSC were repeatedly measured by physiograph(PowerLab). Measurements of those electrical potentials were analyzed by factor analysis. Results : The electric potentials of WSBTM at the left side were divided into four factors. On the other hand those at the right side Were divided into four factors. Conclusion : In conclusion, their electrical potentials at the left and right side were factors which are different from each side. Thus electrical potentials of well and sea points might be the representative meridian to show their characteristics.

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Prediction of the Plastic Strain Ratio Evolution of a Dual-phase Steel (3차원 미세조직에 기반한 잔류응력 하의 이상 조직강의 소성변형률비 예측)

  • Ha, J.;Lee, J.W.;Lee, M. G.;Barlat, F.;Kim, J. H.
    • Transactions of Materials Processing
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    • v.24 no.6
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    • pp.395-399
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    • 2015
  • A microstructure-based finite element simulation was conducted to predict the plastic strain ratio (R-value) of a dual-phase (DP) steel. The representative volume elements (RVEs) concept was adopted for the image-based FE modeling and a 3D model was constructed using sequential 2D images. Each phase was considered with the von-Mises yield criterion and the Swift model. The Swift parameters were defined by the empirical equations based on the chemical composition. The developed model was applied to analyze the effect of residual stress on the R-value and stress distribution. In order to consider the residual stress development after cold rolling, 10 % compression was applied in the thickness direction and unloaded before the tensile stress was applied in the rolling direction. The results showed a reasonable prediction for the R-value evolution: a sharp increase at small strains was well described and a transition followed in the downward direction. The R-value evolution was analyzed using the stress distribution change on the π-plane

Adaptive mesh generation for plane stress problems using error based on element′s representative strain value (요소의 대표 변형률 값에 근거한 에러평가를 이용한 평면응력문제의 적응적 요소망 형성)

  • 정요찬;윤종열;홍승표
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 2003.10a
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    • pp.403-409
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    • 2003
  • The finite element method is one of the most widely used method of structural analysis that has wide applications in diverse fields of engineering and science. The method has been proven effective and reliable in many practical problems. One of the reasons for the methods' popularity is its ease of use, but still the user has to input the finite element mesh which affects the accuracy of the results. The knowledge required to form an effective mesh for a given problem is somewhat complex and for sometime there has been research effort to automate the generation of the mesh and this is called the adaptive mesh generation scheme. A good adaptive mesh scheme seemed to require an accurate assessment of error and generally this requires some additional computation. This paper looks into the possibility of generating adaptive meshes based on representative strain values in each finite element method. The proposed adaptive scheme does not require additional computations other that looking up the data values already computed as finite element analysis results and simple manipulations of these data. Two plane stress problems, a plate with a hole and a deep beam with a concentrated load at the end are considered to show the progress of the improved generation of adaptive meshes using the scheme.

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Calculations of Surface Stresses in Metals Under Mechanical Strains (기계적 변형하에서 금속재료의 표면응력 계산)

  • Kim, Sung-Youb;Earmme, Youn-Young
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.32 no.3
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    • pp.250-257
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    • 2008
  • We calculate the variation of the surface stresses according to uniaxial and biaxial strains in face-centered cubic (FCC) metals. In our study, three mainly observed free surfaces of seven representative FCC metals are considered. Employed method is molecular mechanics, in which the interaction of atoms is described by empirical interatomic potentials. As uniaxial strain increases to tensile direction, the surface stresses on {100} and {110} free surfaces decrease monotonously, while those on {111} surface increase. These tendencies are the same regardless of the species of metals and interatomic potentials employed. However, when the system is under biaxial strain, surface stresses change different according to the surface directions, the species of metals, and even interatomic potentials. On {100} and {111} surfaces, heavy metals (Pt, Au) show the opposite variation to light metals (Ni, Cu). In the cases of Pd and Ag, the surface stresses reveal the opposite tendency, depending on interatomic potentials used.

Probiotic Property and Anti-Obesity Effect of Lactiplantibacillus plantarum KC3

  • Kim, Seulki;Huang, Eunchong;Ji, Yosep;Holzapfel, Wilhelm Helnrich;Lim, Sang-Dong
    • Food Science of Animal Resources
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    • v.42 no.6
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    • pp.996-1008
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    • 2022
  • Lactic acid bacteria are representative probiotics that have beneficial effects on humans. Nineteen strains among the 167 single strains from kimchi was selected and their physiological features were investigated. The selection of a strain was based on strong enzyme (lipase, α-amylase, and α-glucosidase) inhibitory activities and anti-obesity effects in the adipocytes. For the final selection, the strain Lactiplantibacillus plantarum KC3 was tested for its potential as a starter. To assess its functionality, a freeze-dried culture of L. plantarum KC3 was administered to a diet-induced obese mouse model receiving a high-fat diet. The animal group administered with L. plantarum KC3 showed significant body weight loss during the 12-week feeding period compared to the high-fat control group. This study investigated the physiological characteristics of selected strain and evaluated its potential as an anti-obesity probiotic in mice.

Chitinimonas naiadis sp. nov., Isolated from a Freshwater River

  • Padakandla, Shalem Raj;Chae, Jong-Chan
    • Journal of Microbiology and Biotechnology
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    • v.27 no.7
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    • pp.1300-1305
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    • 2017
  • A rod shaped, aerobic, Gram-stain-negative, and motile bacterium, strain $AR2^T$, was isolated from a water sample of Yeongsan river, Republic of Korea. Strain $AR2^T$ clustered closely with the members of the genus Chitinimonas and showed the highest 16S rRNA gene sequence similarity with Chitinimonas prasina $LY03^T$ (96.4%), Chitinimonas viridis $HMD2169^T$ (96.4%), Chitinimonas taiwanensis $cf^T$ (96.2%), and Chitinimonas koreensis $R2A43-10^T$ (94.2%). The predominant fatty acids of strain $AR2^T$ were identified to be summed feature 3 (comprising $C_{16:1}{\omega}7c$ and/or $C_{16:1}{\omega}6c$), $C_{16:0}$, and $C_{10:0}3-OH$. Diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine were found to be the major polar lipids. The genomic DNA G+C content was 60.4 mol%. Based on the polyphasic characterization, the isolated strain $AR2^T$ is described as a representative of a novel species in the genus Chitinimonas, for which the name Chitinimonas naiadis sp. nov. (type strain =$AR2^T$ =KCTC $42755^T$ =JCM $31504^T$) is proposed.