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Sequence variation of necdin gene in Bovidae

  • Peters, Sunday O.;Donato, Marcos De;Hussain, Tanveer;Rodulfo, Hectorina;Babar, Masroor E.;Imumorin, Ikhide G.
    • Journal of Animal Science and Technology
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    • v.60 no.12
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    • pp.32.1-32.10
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    • 2018
  • Background: Necdin (NDN), a member of the melanoma antigen family showing imprinted pattern of expression, has been implicated as causing Prader-Willi symptoms, and known to participate in cellular growth, cellular migration and differentiation. The region where NDN is located has been associated to QTLs affecting reproduction and early growth in cattle, but location and functional analysis of the molecular mechanisms have not been established. Methods: Here we report the sequence variation of the entire coding sequence from 72 samples of cattle, yak, buffalo, goat and sheep, and discuss its variation in Bovidae. Median-joining network analysis was used to analyze the variation found in the species. Synonymous and non-synonymous substitution rates were determined for the analysis of all the polymorphic sites. Phylogenetic analysis were carried out among the species of Bovidae to reconstruct their relationships. Results: From the phylogenetic analysis with the consensus sequences of the studied Bovidae species, we found that only 11 of the 26 nucleotide changes that differentiate them produced amino acid changes. All the SNPs found in the cattle breeds were novel and showed similar percentages of nucleotides with non-synonymous substitutions at the N-terminal, MHD and C-terminal (12.3, 12.8 and 12.5%, respectively), and were much higher than the percentage of synonymous substitutions (2.5, 2.6 and 4.9%, respectively). Three mutations in cattle and one in sheep, detected in heterozygous individuals were predicted to be deleterious. Additionally, the analysis of the biochemical characteristics in the most common form of the proteins in each species show very little difference in molecular weight, pI, net charge, instability index, aliphatic index and GRAVY (Table 4) in the Bovidae species, except for sheep, which had a higher molecular weight, instability index and GRAVY. Conclusions: There is sufficient variation in this gene within and among the studied species, and because NDN carry key functions in the organism, it can have effects in economically important traits in the production of these species. NDN sequence is phylogenetically informative in this group, thus we propose this gene as a phylogenetic marker to study the evolution and conservation in Bovidae.

Performance Indicator and its Application for Evaluation of Construction Infrastructure in North Korea (북한 건설 인프라의 적정 수준 평가를 위한 성능지표 개발 및 적용 연구)

  • Kim, Minju;Kim, Sangyup;Oh, Sangmin;Cho, Hunhee;Kang, Kyung-In
    • Korean Journal of Construction Engineering and Management
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    • v.20 no.1
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    • pp.62-72
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    • 2019
  • Future prospects for the North Korean construction market created by reunification of the Korean Peninsula has consistently been recognized as highly promising. Although rehabilitation projects of the deteriorating construction facilities in North Korea are expected to serve as new source of growth engine to South Korea in future, the status quo of each infrastructure in the North are described verbosely with signs of subjective translation. There has been limited approach on depicting the condition and performance of the North Korean facilities based on quantitative and systematic approach. Thus, this study has been conducted under a twofold purpose. First, this study proposes a performance indicator for identifying the status quo of the major construction facilities in North Korea based on a quantitative approach. Second, this study applies the developed indicator to visualize the results on the conditions of each facility in the form of a report card. This expected to serve as a basis on determining the appropriate level of the construction infrastructure in North Korea and estimating the range of rehabilitation projects along with the investment cost.

Text Mining Analysis Technique on ECDIS Accident Report (텍스트 마이닝 기법을 활용한 ECDIS 사고보고서 분석)

  • Lee, Jeong-Seok;Lee, Bo-Kyeong;Cho, Ik-Soon
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.4
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    • pp.405-412
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    • 2019
  • SOLAS requires that ECDIS be installed on ships of more than 500 gross tonnage engaged in international navigation until the first inspection arriving after July 1, 2018. Several accidents related to the use of ECDIS have occurred with its installation as a new major navigation instrument. The 12 incident reports issued by MAIB, BSU, BEAmer, DMAIB, and DSB were analyzed, and the cause of accident was determined to be related to the operation of the navigator and the ECDIS system. The text was analyzed using the R-program to quantitatively analyze words related to the cause of the accident. We used text mining techniques such as Wordcloud, Wordnetwork and Wordweight to represent the importance of words according to their frequency of derivation. Wordcloud uses the N-gram model as a way of expressing the frequency of used words in cloud form. As a result of the uni-gram analysis of the N-gram model, ECDIS words were obtained the most, and the bi-gram analysis results showed that the word "Safety Contour" was used most frequently. Based on the bi-gram analysis, the causative words are classified into the officer and the ECDIS system, and the related words are represented by Wordnetwork. Finally, the related words with the of icer and the ECDIS system were composed of word corpus, and Wordweight was applied to analyze the change in corpus frequency by year. As a result of analyzing the tendency of corpus variation with the trend line graph, more recently, the corpus of the officer has decreased, and conversely, the corpus of the ECDIS system is gradually increasing.

Topographical Analysis of the Potholes in Jakgwaecheon Stream in Ulsan (울산 작괘천의 포트홀에 관한 지형분석)

  • Kim, Tae-hyeong;Kong, Dal-yong;Lim, Jong-deock;Jung, Seung-ho;Yu, Yeong-wan
    • Korean Journal of Heritage: History & Science
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    • v.46 no.3
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    • pp.68-77
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    • 2013
  • This report is based on the investigation of potholes which are formed by fluvial erosion. A pothole is called so because it is a hole that looks like a coffeepot. The results of previous studies are applied to the 'Jakgwaecheon Porthole' of this study. The study is focused on the dimension and morphology of the Pothole and investigates the effects of stream sediments, river flow, geological structural lines, etc. on the formation of potholes. As a result of measuring 61 potholes in this area, we recognized that the elliptical dish-shaped cross sections are dominant and inferred that their longitudinal direction on the plain is affected by the direction of the stream flow. Also, 'Jakgwaecheon Pothole' is very characterized in terms of scale and morphology. Furthermore, it is harmonious with the beautiful landscape, humanity, and historical values and it can be suggested that it is qualified to be registered as a geoheritage structure.

The Research report of ethnic customs in Dong-shan(東山) Yao(瑤) family (중국(中國) 광서성(廣西省) 전주현(全州縣) 동산요족(東山瑤族) 민속문화(民俗文化) 조사(調査) 약보고(略報告))

  • Park, dae-nam;Hyun, chang-ju
    • Korean Journal of Heritage: History & Science
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    • v.37
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    • pp.169-211
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    • 2004
  • As for the "Guang-Xi(廣西)", "Dong-Shan(東山)", "Yao-Ju(瑤族)", folklore culture to achieve the substratum (New Year manners and customs, a passage rites, folk belief, dwelling folklore, agriculture and a farming machine) generally very received an influence of "Han-zu(漢族)" for the reason that an interchange was active early with "Han-zu(漢族)". However, a traditional form of "Yao-Ju(瑤族)" is covered the base with. Even if it is a national holiday commemorateing the birth of the "Pan-Gu(盤古)" which is ancestors of all "Yao-zu(瑤族)" during New Year manners and customs, songs as "ku-jia(哭嫁)" consisting at the time of marriage during a lot of ritual, "zhaoxu-hun (招婿婚)" and the "liangtou-che(兩頭扯)" marriages which are a classic marriage of "Yao-zu(瑤族)", a ritual format, master "Shi-Gong(師公)" of faith of "Yao-zu(瑤族)" are. Also, a difference is in dwelling folklore related to construction or this very much in "Han-zu(漢族)" and the various sides. It is the part where toilets to use are quite different from the Korean race in a tool, the outside written with the dwelling formal characteristics that are structure, "the ceremony of putting up the ridge beam" in, for example, two folds. Agriculture and a farming machine are basically similar to it of the Korean race, but it is a degree with some transformation by environment and the local cause.

An Analysis of Newspaper Coverage of Korean Movie Stars : Focusing on the Image of Movie Stars and Reporting Trend (신문의 한국 영화스타 보도 내용분석 : 영화스타의 이미지와 보도 경향 중심으로)

  • Tae, Bo-Ra
    • The Journal of the Korea Contents Association
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    • v.19 no.9
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    • pp.535-549
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    • 2019
  • The purpose of this study was to examine what kind of images were presented on movie stars in the newspaper. For the purpose, we classified the time period according to the movie industry and media trend, selected representative stars by period, and collected 798 related articles reported in newspapers. As a result of analyzing the reporting trend, domestic and foreign topics, news format, and gender difference in collected movie star articles, it was found that the image of movie stars reproduced in newspaper articles had mostly neutral images that do not represent specific gender. Since the 2000s, news coverage was changed to reproduce various images rather than being fixed to particular images, and the subject of report became more diversified through comparison of domestic and foreign topics. In addition, articles in the form of book review decreased and the interview-type articles increased in number, and in the case of male movie stars, the proportion of articles based on works was high in comparison to female movie stars. This study has significance in that it explored the changes in the process of reproducing star images diachronically from the initial stage of stars to the modern times. And it is hoped that this study will serve as basic data for the follow-up studies on the process of reproducing various images in the multi-media era.

Survey on the Perception of Stakeholders on the EIA System in Korea (한국 환경영향평가 제도에 대한 이해관계자의 인식 조사)

  • Kim, Minkyung;Lee, Sangdon
    • Journal of Environmental Impact Assessment
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    • v.30 no.1
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    • pp.49-60
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    • 2021
  • Environmental impact assessment (EIA) practitioners play a very pivotal role in establishing EIA policies, and when implementing EIA environmental conflicts can be prevented and resolved by sharing information with stakeholders and coordinating opinions. For this reason, grasping the perceptions of stakeholders including practitioners about the overall system, such as EIA policies and implementation, can be helpful in setting improvement directions and policy directions for EIA. However, there is an insufficient information on the perception and understanding of stakeholders about the EIA system and operation currently in effect in Korea. Therefore, this study diagnoses operational and procedural problems for the EIA system, which is a decision-making tool and a precautionary technique that can minimize adverse effects through the environmental information analysis method, and improvement points and systems of the EIA system in the future. We tried to find a complement of an online survey of 37 questions,responses from 95 responses from stakeholders of EIA were summarized. Stakeholders were aware of the problems of the operation of the current system and the preparation of the evaluation form, and this was reflected. Period and cost of preparation of EIS (49%), the introduction of a new method (26%) and the items of collecting opinions and conflict management (41%), which showed high negative response rates (dissatisfied and very dissatisfied), are considered to be areas that we need to supplement further in the future. As society develops rapidly, the system needs to be supplemented accordingly, and policy improvement efforts are needed for items with high negative responses as a result of the survey.

Current Status of X-ray CT Based Non Destructive Characterization of Bentonite as an Engineered Barrier Material (공학적방벽재로서 벤토나이트 거동의 X선 단층촬영 기반 비파괴 특성화 현황)

  • Diaz, Melvin B.;Kim, Joo Yeon;Kim, Kwang Yeom;Lee, Changsoo;Kim, Jin-Seop
    • Tunnel and Underground Space
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    • v.31 no.6
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    • pp.400-414
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    • 2021
  • Under high-level radioactive waste repository conditions, bentonite as an engineered barrier material undergoes thermal, hydrological, mechanical, and chemical processes. We report the applications of X-ray Computed Tomography (CT) imaging technique on the characterization and analysis of bentonite over the past decade to provide a reference of the utilization of this technique and the recent research trends. This overview of the X-ray CT technique applications includes the characterization of the bentonite either in pellets or powder form. X-ray imaging has provided a means to extract grain information at the microscale and identify crack networks responsible for the pellets' heterogeneity. Regarding samples of pellets-powder mixtures under hydration, X-ray CT allowed the identification and monitoring of heterogeneous zones throughout the test. Some results showed how zones with pellets only swell faster compared to others composed of pellets and powder. Moreover, the behavior of fissures between grains and bentonite matrix was observed to change under drying and hydrating conditions, tending to close during the former and open during the latter. The development of specializing software has allowed obtaining strain fields from a sequence of images. In more recent works, X-ray CT technique has served to estimate the dry density, water content, and particle displacement at different testing times. Also, when temperature was added to the hydration process of a sample, CT technology offered a way to observe localized and global density changes over time.

Web viewer for sharing of prosthesis design between laboratory and clinic: Case report (웹뷰어를 이용한 기공실과 진료실 측 간의 보철물 설계 형태의 공유: 증례 보고)

  • Jang, Sung Won;Lee, Ho Jin;Kim, So-Yeun;Lee, Du-Hyeong
    • The Journal of Korean Academy of Prosthodontics
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    • v.60 no.3
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    • pp.276-282
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    • 2022
  • Close communication between clinicians and dental technicians is an important factor in providing successful prostheses. The exchange of opinions with laboratories has mainly been in the form of written prescriptions and a photos, but it has been reported that information transmission may be limited. Currently, as digital technology-based prosthesis fabrication is common, 3D image objects can be stored on the web and can be easily viewed through a mobile web browser. In this article, we introduce cases where the design of the prosthesis was improved by designing the prosthesis using CAD software and reviewing the prosthesis designed with the clinical side through a web viewer. Through this protocol, it was possible to improve the occlusal surface and crown contour, the opposing teeth condition, the size of the gingival embrasure, and the shape of pontic. The process of sharing, discussing, and modifying the prosthesis design with the clinician and technician through a web viewer contributes to reflecting the diversity of oral conditions and individualized needs, thereby helping to make functional and esthetic prostheses.

Maxillary overdenture restoration using functionally generated path technique: a case report (Functionally generated path technique을 이용한 편악 피개의치 수복 증례)

  • Jo, Yukyung;Lee, Younghoo;Hong, Seoung-Jin;Noh, Kwantae;Pae, Ahran;Kim, Hyeong-Seob;Kwon, Kung-Rock;Paek, Janghyun
    • The Journal of Korean Academy of Prosthodontics
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    • v.60 no.1
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    • pp.55-62
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    • 2022
  • The functionally generated path (FGP) technique, first described by Meyer in 1933, is a method in the oral cavity to use the movement pathway formed by the opposing cusps within the border movement of the mandible. Using this method, an appropriate occlusal shape can be given to the patient. In this case, the FGP technique was selected to provide a bilateral balanced occlusion when restoring the edentulous maxilla that opposes the natural mandibular teeth with irregular arrangement with overdentures. In addition, in order to precisely form the occlusal surface of the posterior region with the FGP technique and to reduce the attrition of denture teeth, zirconia denture teeth, not conventional resin artificial teeth, were individually manufactured. After treatment with these materials and methods, satisfactory results were obtained for both the operator and the patient.